3'-Phosphoadenosine 5'-phosphosulfate binding site of flavonol 3-sulfotransferase studied by affinity chromatography and 31P NMR.

abstract：:The occurrence of 8-oxo-7,8-dihydroguanine (OG) in the genome, as one of the major DNA oxidative damages, has been implicated in an array of biological processes, ranging from mutagenesis to transcriptional regulation. Genome-wide mapping of oxidative damages could shed light on the underlying cellular mechanism. In t...

journal_title：Biochemistry

authors： Fang Y,Zou P

更新日期：2020-01-14 00:00:00

abstract：:Polylysine promoted extensive membrane mixing of liposomes only if the buffer pH was below the pKa of the lysyl residues. This observation suggested that fusion could be regulated in a physiological pH range if the homopolymer of L-histidine was substituted as fusogen. Microgram quantities of polyhistidine were added ...

journal_title：Biochemistry

authors： Uster PS,Deamer DW

更新日期：1985-01-01 00:00:00

abstract：:Static FTIR light-induced difference spectra have been recorded for reaction centers from Rhodopseudomonas viridis in the following charge-separated states: P+QA(-)-PQA, P+QB(-)-PQB, I(-)-I, I-QA(-)-IQA, and I-QA(2-)-IQA. A comparison of the I(-)-I difference spectra with the I-QA(-)-IQA difference spectra reveals new...

journal_title：Biochemistry

authors： Buchanan S,Michel H,Gerwert K

更新日期：1992-02-11 00:00:00

abstract：:The photosynthetic antenna of Chloroflexus aurantiacus includes bacteriochlorophyll (BChl) C740 and BChl a792, both of which occur in chlorosomes, and B808-866 (containing BChl a808 and BChl a866), which is membrane-located (subscripts refer to near-infrared absorption maxima in vivo). BChl a792 is thought to mediate ...

journal_title：Biochemistry

authors： Brune DC,King GH,Infosino A,Steiner T,Thewalt ML,Blankenship RE

更新日期：1987-12-29 00:00:00

abstract：:The photoautotrophic freshwater cyanobacterium Synechococcus elongatus is widely used as a chassis for biotechnological applications as well as a photosynthetic bacterial model. In this study, a method for expanding the genetic code of this cyanobacterium has been established, thereby allowing the incorporation of unn...

journal_title：Biochemistry

authors： Chemla Y,Friedman M,Heltberg M,Bakhrat A,Nagar E,Schwarz R,Jensen MH,Alfonta L

更新日期：2017-04-25 00:00:00

abstract：:The class C serine beta-lactamase of Enterobacter cloacae P99 was inhibited by a series of aryl methylphosphonate monoester monoanions. The effectiveness of these inhibitors was promoted by an acylamido substituent on the methyl group and a good leaving group at phosphorus. The former preference suggests that noncoval...

journal_title：Biochemistry

authors： Rahil J,Pratt RF

更新日期：1992-06-30 00:00:00

abstract：:Insulin-stimulated glucose disposal in skeletal muscle proceeds predominantly through a nonoxidative pathway with glycogen synthase as a rate-limiting enzyme, yet the mechanisms for insulin activation of glycogen synthase are not understood despite years of investigation. Isolation of putative insulin second messenger...

journal_title：Biochemistry

authors： Brautigan DL,Brown M,Grindrod S,Chinigo G,Kruszewski A,Lukasik SM,Bushweller JH,Horal M,Keller S,Tamura S,Heimark DB,Price J,Larner AN,Larner J

更新日期：2005-08-23 00:00:00

abstract：:Annexin V (AxV) is a member of a family of proteins that exhibit functionally relevant Ca2+-dependent binding to anionic phospholipid membranes. Protein structure and stability as a function of Ca2+ and phospholipids was studied by bulk phase infrared (IR) spectroscopy and by IR reflection-absorption spectroscopy (IRR...

journal_title：Biochemistry

authors： Wu F,Flach CR,Seaton BA,Mealy TR,Mendelsohn R

更新日期：1999-01-12 00:00:00

abstract：:The observed regioselectivity of the homolytic cleavage of S-adenosylmethionine (SAM) by the radical SAM enzymes is modeled by free radical displacement reactions at sulfoxide centers. These displacements are also regioselective, in direct consequence of the reaction mechanism. The selectivity in the radical SAM react...

journal_title：Biochemistry

authors： Kampmeier JA

更新日期：2010-12-28 00:00:00

abstract：:Gap junctions, transmembrane protein channels that directly connect the cytoplasm of neighboring cells and enable the exchange of molecules between cells, are a promising new frontier for therapeutic delivery. Specifically, cell-derived lipid vesicles that contain functional gap junction channels, termed Connectosomes...

journal_title：Biochemistry

authors： Gadok AK,Zhao C,Meriwether AI,Ferrati S,Rowley TG,Zoldan J,Smyth HDC,Stachowiak JC

更新日期：2018-01-09 00:00:00

abstract：:In order to investigate the changes of protonation or environment of carboxylic residues occurring upon photoreduction of the secondary quinone acceptor (QB) in the reaction center (RC) of the photosynthetic bacteria Rhodobacter sphaeroides 2.4.1., we have performed light-induced Fourier transform infrared (FTIR) spec...

journal_title：Biochemistry

authors： Nabedryk E,Breton J,Hienerwadel R,Fogel C,Mäntele W,Paddock ML,Okamura MY

更新日期：1995-11-14 00:00:00

abstract：:Plexins are transmembrane proteins that serve as guidance receptors during angiogenesis, lymphangiogenesis, neuronal development, and zebrafish fin regeneration, with a putative role in cancer metastasis. Receptor dimerization or clustering, induced by extracellular ligand binding but modulated in part by the plexin t...

journal_title：Biochemistry

authors： Barton R,Khakbaz P,Bera I,Klauda JB,Iovine MK,Berger BW

更新日期：2016-09-06 00:00:00

abstract：:Glycopeptidolipids (GPLs) are specific constituents of mycobacteria known as opportunistic pathogens. The influence of the carbohydrate moiety on GPL-induced membrane alterations was examined with GPLs bearing 1-5 sugar residues (GPL-1 to GPL-5) and a sulfated GPL (S-GPL-2). GPLs decreased the ADP/O ratio and increase...

journal_title：Biochemistry

authors： Lopez-Marin LM,Quesada D,Lakhdar-Ghazal F,Tocanne JF,Lanéelle G

更新日期：1994-06-14 00:00:00

abstract：:Misfolded proteins in the endoplasmic reticulum (ER) are degraded by N-terminal threonine proteases within the 26S proteasome. Each protease is formed by an activated beta subunit, beta5/X, beta1/Y, or beta2/Z, that exhibits chymotrypsin-like, peptidylglutamyl-peptide hydrolyzing, or trypsin-like activity, respectivel...

journal_title：Biochemistry

authors： Oberdorf J,Carlson EJ,Skach WR

更新日期：2001-11-06 00:00:00

abstract：:DnaB helicase is responsible for unwinding duplex DNA during chromosomal DNA replication and is an essential component of the DNA replication apparatus in Escherichia coli. We have analyzed the mechanism of binding of single-stranded DNA (ssDNA) by the DnaB x DnaC complex and DnaB helicase. Binding of ssDNA to DnaB he...

journal_title：Biochemistry

authors： Biswas SB,Biswas-Fiss EE

更新日期：2006-09-26 00:00:00

abstract：:Prion diseases are associated with the conversion of the normal prion protein, PrP(C), to the infectious disease form PrP(Sc). Discrimination between these isoforms would significantly enhance diagnosis of these diseases, and it has recently been reported that PrP(Sc) is specifically recognized by the serine protease ...

journal_title：Biochemistry

authors： Ellis V,Daniels M,Misra R,Brown DR

更新日期：2002-06-04 00:00:00

abstract：:Ultraviolet absorption techniques were used to study the thermodynamics of duplex formation for a DNA decamer, d(GCGAAAAGCG).d(CGCTTTTCGC), and a series of related duplexes, each of which contains a bulged base centered in the A.T tract. Thermodynamic parameters were obtained from nonlinear least-squares fits of the m...

journal_title：Biochemistry

authors： LeBlanc DA,Morden KM

更新日期：1991-04-23 00:00:00

abstract：:Helix-helix interactions within membranes are dominated by van der Waals packing motifs and side chain-side chain hydrogen bond formation, which act in tandem to determine the residues that comprise the interface between two given helices. To explore in a systematic manner the tertiary contacts between transmembrane h...

journal_title：Biochemistry

authors： Johnson RM,Heslop CL,Deber CM

更新日期：2004-11-16 00:00:00

abstract：:Cytochrome bc(1), a key enzyme of biological energy conversion, generates or uses a proton motive force through the Q cycle that operates within the two chains of cofactors that embed two catalytic quinone oxidation/reduction sites, the Q(o) site and the Q(i) site. The Q(o) site relies on the joint action of two cofac...

journal_title：Biochemistry

authors： Borek A,Sarewicz M,Osyczka A

更新日期：2008-11-25 00:00:00

abstract：:The role of histidine in catalysis by prostaglandin H synthase has been investigated using chemical modification with diethyl pyrocarbonate (DEPC), an agent that has been found to rather selectively derivatize histidine residues in proteins under mild conditions. Incubation of the synthase apoprotein with DEPC at pH 7...

journal_title：Biochemistry

authors： Zhang X,Tsai AL,Kulmacz RJ

更新日期：1992-03-10 00:00:00

abstract：:In our previous papers, seven structural genes (NQO1-7) of the energy-transducing NADH-quinone (Q) oxidoreductase of Paracoccus denitrificans were characterized [Xu, X., Matsuno-Yagi, A., & Yagi, T. (1991a) Biochemistry 30, 8678-8684; (1991b) Biochemistry 30, 6422-6428; (1992a) Biochemistry 31, 6925-6932; (1992b) Arch...

journal_title：Biochemistry

authors： Xu X,Matsuno-Yagi A,Yagi T

更新日期：1993-01-26 00:00:00

abstract：:Several strains of Sphingobium chlorophenolicum have been isolated from soil that was heavily contaminated with pentachlorophenol (PCP), a toxic pesticide introduced in the 1930s. S. chlorophenolicum appears to have assembled a poorly functioning pathway for degradation of PCP by patching enzymes recruited via two ind...

journal_title：Biochemistry

authors： Hlouchova K,Rudolph J,Pietari JM,Behlen LS,Copley SD

更新日期：2012-05-08 00:00:00

abstract：:Proteolytic processing of the transmembrane domain of the amyloid precursor protein (APP) is a key component of Alzheimer's disease pathogenesis. Using C-terminally tagged APP derivatives, we have identified by amino-terminal sequencing a novel cleavage site of APP, at Leu-49, distal to the gamma-secretase site. This ...

journal_title：Biochemistry

authors： Weidemann A,Eggert S,Reinhard FB,Vogel M,Paliga K,Baier G,Masters CL,Beyreuther K,Evin G

更新日期：2002-02-26 00:00:00

abstract：:To identify how many rhodopsin intermediates interact with retinal G-protein transducin, the photobleaching process of chicken rhodopsin has been investigated in the presence or absence of transducin by means of time-resolved low-temperature spectroscopy. Singular value decomposition (SVD) analysis of the spectral dat...

journal_title：Biochemistry

authors： Tachibanaki S,Imai H,Mizukami T,Okada T,Imamoto Y,Matsuda T,Fukada Y,Terakita A,Shichida Y

更新日期：1997-11-18 00:00:00

abstract：:The mutagenic and genotoxic properties of 1,N6-ethenoadenine (epsilon Ade), 3,N4-ethenocytosine (epsilon Cyt), and 4-amino-5-(imidazol-2-yl)imidazole (beta) were investigated in vivo. The former two modified bases are known DNA adducts formed by the human carcinogen vinyl chloride; beta is formed by pyrimidine ring-op...

journal_title：Biochemistry

authors： Basu AK,Wood ML,Niedernhofer LJ,Ramos LA,Essigmann JM

更新日期：1993-11-30 00:00:00

abstract：:Anatoxin-a and homoanatoxin-a are two potent cyanobacterial neurotoxins. We recently reported the identification of the gene cluster responsible for the biosynthesis of these toxins as well as the in-vitro reconstitution of the first steps of this biosynthesis. We now report experimental evidence supporting the propos...

journal_title：Biochemistry

authors： Mann S,Lombard B,Loew D,Méjean A,Ploux O

更新日期：2011-08-23 00:00:00

abstract：:The helical structure of double-stranded DNA is destabilized by increasing temperature. Above a critical temperature (the melting temperature), the two strands in duplex DNA become fully separated. Below this temperature, the structural effects are localized. Using tethered particle motion in a temperature-controlled ...

journal_title：Biochemistry

authors： Driessen RP,Sitters G,Laurens N,Moolenaar GF,Wuite GJ,Goosen N,Dame RT

更新日期：2014-10-21 00:00:00

abstract：:31P NMR spectra were obtained from poly(dA-dT) . poly(dA-dT) fibers which gave an X-ray diffraction pattern similar to that of the A form of natural deoxyribonucleic acid (DNA). The analysis of the line shape indicated that the A form of poly(dA-dT) . poly(dA-dT) has a single uniform backbone conformation; the orienta...

journal_title：Biochemistry

authors： Shindo H,Wooten JB,Zimmerman SB

更新日期：1981-02-17 00:00:00

abstract：:The lipid fatty acid composition of the cytoplasmic membranes of Escherichia coli can be varied by growing an unsaturated fatty acid auxotroph in the presence of different fatty acid supplements. Electron spin resonance (ESR) studies of spin-label partitioning into the cytoplasmic membranes of different lipid fatty ac...

journal_title：Biochemistry

authors： Linden CD,Blasie JK,Fox CF

更新日期：1977-04-19 00:00:00

abstract：:Arachidonic acid metabolism by lipoxygenases and cytochrome P450 monooxygenases produces regioisomeric hydroperoxyeicosatetraenoic acids (HPETEs), hydroxyeicosatetraenoic acids (HETEs), epoxyeicosatrienoic acids (EETs), and dihydroxyeicosatrienoic acids (DHETs), which serve as components of cell signaling cascades. In...

journal_title：Biochemistry

authors： Widstrom RL,Norris AW,Spector AA

更新日期：2001-01-30 00:00:00