Stimulation of transcript elongation requires both the zinc finger and RNA polymerase II binding domains of human TFIIS.


:The eukaryotic transcriptional factor TFIIS enhances transcript elongation by RNA polymerase II. Here we describe two functional domains in the 280 amino acid human TFIIS protein: residues within positions 100-230 are required for binding to polymerase, and residues 230-280, which form a zinc finger, are required in conjunction with the polymerase binding region for transcriptional stimulation. Interestingly, a mutant TFIIS with only the polymerase binding domain actually inhibits transcription, whereas a mutant in which the polymerase binding and zinc finger domains are separated by an octapeptide is only weakly active. The zinc finger itself has no effect on transcription, but in contrast to the wild-type protein, it binds to oligonucleotides. These findings suggest that TFIIS may interact with RNA polymerase II such that the normally masked zinc finger can specifically contact nucleotides in the transcription elongation zone at a position juxtaposed to the polymerization site.






Agarwal K,Baek KH,Jeon CJ,Miyamoto K,Ueno A,Yoon HS




Has Abstract


1991-08-06 00:00:00












  • Light inhibition of bovine retinal rod guanylyl cyclase mediated by beta gamma-transducin.

    abstract::Photoreceptor guanylyl cyclase (ROS-GC), converting GTP into cGMP and pyrophosphate, is a key enzyme in the regulation of the visual transduction cascade. ROS-GC requires GC-activating proteins (GCAPs) and low free [Ca] for full activity. We found that when choline or potassium were the major cations present, light ca...


    pub_type: 杂志文章


    authors: Wolbring G,Baehr W,Palczewski K,Schnetkamp PP

    更新日期:1999-03-02 00:00:00

  • The Delta 14 mutation of human cardiac troponin T enhances ATPase activity and alters the cooperative binding of S1-ADP to regulated actin.

    abstract::The complex of tropomyosin and troponin binds to actin and inhibits activation of myosin ATPase activity and force production of striated muscles at low free Ca(2+) concentrations. Ca(2+) stimulates ATP activity, and at subsaturating actin concentrations, the binding of NEM-modified S1 to actin-tropomyosin-troponin in...


    pub_type: 杂志文章


    authors: Gafurov B,Fredricksen S,Cai A,Brenner B,Chase PB,Chalovich JM

    更新日期:2004-12-07 00:00:00

  • The adhesive specificity of the soluble human lectin, IgE-binding protein, toward lipid-linked oligosaccharides. Presence of the blood group A, B, B-like, and H monosaccharides confers a binding activity to tetrasaccharide (lacto-N-tetraose and lacto-N-ne

    abstract::The immunoglobulin E-binding protein, epsilon BP (also known as CBP35, Mac-2, L-34, and L-29), is a beta-galactoside-binding protein of approximately 30 kDa and a member of the animal lectin family termed S-type or S-Lac. Multiple biological activities have been attributed to this lectin such as mediation of IgE bindi...


    pub_type: 杂志文章


    authors: Feizi T,Solomon JC,Yuen CT,Jeng KC,Frigeri LG,Hsu DK,Liu FT

    更新日期:1994-05-24 00:00:00

  • Functional and structural characterization of an unusual cofactor-independent oxygenase.

    abstract::The vast majority of characterized oxygenases use bound cofactors to activate molecular oxygen to carry out oxidation chemistry. Here, we show that an enzyme of unknown activity, RhCC from Rhodococcus jostii RHA1, functions as an oxygenase, using 4-hydroxyphenylenolpyruvate as a substrate. This unique and complex reac...


    pub_type: 杂志文章


    authors: Baas BJ,Poddar H,Geertsema EM,Rozeboom HJ,de Vries MP,Permentier HP,Thunnissen AM,Poelarends GJ

    更新日期:2015-02-10 00:00:00

  • Thermodynamic investigation into the mechanisms of proton-coupled electron transfer events in heme protein maquettes.

    abstract::To study the engineering requirements for proton pumping in energy-converting enzymes such as cytochrome c oxidase, the thermodynamics and mechanisms of proton-coupled electron transfer in two designed heme proteins are elucidated. Both heme protein maquettes chosen, heme b-[H10A24]2 and heme b-[delta7-His]2, are four...


    pub_type: 杂志文章


    authors: Reddi AR,Reedy CJ,Mui S,Gibney BR

    更新日期:2007-01-09 00:00:00

  • Formation and function of the Manganese(IV)/Iron(III) cofactor in Chlamydia trachomatis ribonucleotide reductase.

    abstract::The beta(2) subunit of a class Ia or Ib ribonucleotide reductase (RNR) is activated when its carboxylate-bridged Fe(2)(II/II) cluster reacts with O(2) to oxidize a nearby tyrosine (Y) residue to a stable radical (Y(*)). During turnover, the Y(*) in beta(2) is thought to reversibly oxidize a cysteine (C) in the alpha(2...


    pub_type: 杂志文章,评审


    authors: Jiang W,Yun D,Saleh L,Bollinger JM Jr,Krebs C

    更新日期:2008-12-30 00:00:00

  • Functional characterization of an NADPH dependent 2-alkyl-3-ketoalkanoic acid reductase involved in olefin biosynthesis in Stenotrophomonas maltophilia.

    abstract::OleD is shown to play a key reductive role in the generation of alkenes (olefins) from acyl thioesters in Stenotrophomonas maltophilia. The gene coding for OleD clusters with three other genes, oleABC, and all appear to be transcribed in the same direction as an operon in various olefin producing bacteria. In this stu...


    pub_type: 杂志文章


    authors: Bonnett SA,Papireddy K,Higgins S,del Cardayre S,Reynolds KA

    更新日期:2011-11-08 00:00:00

  • Isolation and characterization of somatolactin, a new protein related to growth hormone and prolactin from Atlantic cod (Gadus morhua) pituitary glands.

    abstract::The characterization of cod somatolactin (SL), a new pituitary protein belonging to the growth hormone/prolactin family, is described. Cod SL has a molecular weight of 26 kDa and consists of 209 amino acids, of which eight are Cys. The protein has three disulfide bonds between residues Cys5-Cys15, Cys65-Cys181, and Cy...


    pub_type: 杂志文章


    authors: Rand-Weaver M,Noso T,Muramoto K,Kawauchi H

    更新日期:1991-02-12 00:00:00

  • Tropomyosin stabilizes the pointed end of actin filaments by slowing depolymerization.

    abstract::Tropomyosin is postulated to confer stability to actin filaments in nonmuscle cells. We have found that a nonmuscle tropomyosin isolated from the intestinal epithelium can directly stabilize actin filaments by slowing depolymerization from the pointed, or slow-growing, filament end. Kinetics of elongation and depolyme...


    pub_type: 杂志文章


    authors: Broschat KO,Weber A,Burgess DR

    更新日期:1989-10-17 00:00:00

  • An extracellular loop region of the serotonin transporter may be involved in the translocation mechanism.

    abstract::The serotonin transporter (SERT) is a member of a highly homologous family of proteins responsible for the reuptake of biogenic amines from the synaptic cleft. We took advantage of native restriction sites in SERT to construct a chimeric transporter containing a small (34 amino acid) region of the norepinephrine trans...


    pub_type: 杂志文章


    authors: Stephan MM,Chen MA,Penado KM,Rudnick G

    更新日期:1997-02-11 00:00:00

  • Inhibitory copper binding site on the spinach cytochrome b6f complex: implications for Qo site catalysis.

    abstract::The isolated cytochrome (cyt) b(6)f complex from spinach is inhibited by Cu(2+) with a K(D) of about 1 microM at pH 7.6 in the presence of 1.6 microM decyl-plastoquinol (C(10)-PQH(2)) as a substrate. Inhibition was competitive with respect to C(10)-PQH(2) but noncompetitive with respect to horse heart cyt c or plastoc...


    pub_type: 杂志文章


    authors: Rao BK,Tyryshkin AM,Roberts AG,Bowman MK,Kramer DM

    更新日期:2000-03-28 00:00:00

  • Solution structure and cell selectivity of piscidin 1 and its analogues.

    abstract::Piscidin 1 (Pis-1) is a novel cytotoxic peptide with a cationic alpha-helical structure that was isolated from the mast cells of hybrid striped bass [Silphaduang, U., and Noga, E. J. (2001) Nature 414, 268-269]. Pis-1 is not selective for bacterial versus mammalian cells. In the present study, to develop novel antibio...


    pub_type: 杂志文章


    authors: Lee SA,Kim YK,Lim SS,Zhu WL,Ko H,Shin SY,Hahm KS,Kim Y

    更新日期:2007-03-27 00:00:00

  • Fidelity of the RNA-dependent DNA synthesis exhibited by the reverse transcriptases of human immunodeficiency virus types 1 and 2 and of murine leukemia virus: mispair extension frequencies.

    abstract::Human immunodeficiency viruses type 1 and 2 (HIV-1 and HIV-2, respectively) exhibit extensive genetic variations. It was postulated that much of this genetic variability stems from the low fidelity of the reverse transcription step. Both HIV reverse transcriptases (RTs) were shown to be particularly error-prone during...


    pub_type: 杂志文章


    authors: Bakhanashvili M,Hizi A

    更新日期:1992-10-06 00:00:00

  • NSC 622124 inhibits human Eg5 and other kinesins via interaction with the conserved microtubule-binding site.

    abstract::Kinesin-5 proteins are essential for formation of a bipolar mitotic spindle in most and, perhaps all, eukaryotic cells. Several Kinesin-5 proteins, notably the human version, HsEg5, are targets of a constantly expanding group of small-molecule inhibitors, which hold promise both as tools for probing mechanochemical tr...


    pub_type: 杂志文章


    authors: Learman SS,Kim CD,Stevens NS,Kim S,Wojcik EJ,Walker RA

    更新日期:2009-03-03 00:00:00

  • Activation of GlcNAc-P-P-dolichol synthesis by mannosylphosphoryldolichol is stereospecific and requires a saturated alpha-isoprene unit.

    abstract::Exogenous mannosylphosphoryldolichol (Man-P-Dol) has previously been shown to stimulate UDP-GlcNAc:dolichyl phosphate N-acetylglucosamine 1-phosphate transferase (GPT1), the enzyme catalyzing the biosynthesis of N-acetylglucosaminylpyrophosphoryldolichol (GlcNAc-P-P-Dol). To define the structural specificity of the ma...


    pub_type: 杂志文章


    authors: Kean EL,Rush JS,Waechter CJ

    更新日期:1994-08-30 00:00:00

  • Partial denaturation of transthyretin is sufficient for amyloid fibril formation in vitro.

    abstract::Amyloid diseases are caused by the self-assembly of a given protein into an insoluble cross-beta-sheet quaternary structural form which is pathogenic. An understanding of the biochemical mechanism of amyloid fibril formation should prove useful in understanding amyloid disease. Toward this end, a procedure for the con...


    pub_type: 杂志文章


    authors: Colon W,Kelly JW

    更新日期:1992-09-15 00:00:00

  • Complex of human apolipoprotein C-1 with phospholipid: thermodynamic or kinetic stability?

    abstract::Thermal unfolding of discoidal complexes of apolipoprotein (apo) C-1 with dimyristoyl phosphatidylcholine (DMPC) reveals a novel mechanism of lipoprotein stabilization that is based on kinetics rather than thermodynamics. Far-UV CD melting curves recorded at several heating/cooling rates from 0.047 to 1.34 K/min show ...


    pub_type: 杂志文章


    authors: Gursky O,Ranjana,Gantz DL

    更新日期:2002-06-11 00:00:00

  • Fucoxanthin-chlorophyll a/c light-harvesting complexes of Laminaria saccharina: partial amino acid sequences and arrangement in thylakoid membranes.

    abstract::The N-terminus of the major polypeptide component of the light-harvesting complex (LHC) from the brown alga Laminaria saccharina is blocked. Two partial sequences, one near the N-terminus and the other near the C-terminus, have been obtained by chemical cleavage with acetic acid and N-chlorosuccinimide. Four peptides ...


    pub_type: 杂志文章


    authors: Douady D,Rousseau B,Caron L

    更新日期:1994-03-22 00:00:00

  • Complete amino acid sequence of the 4Fe-4S, thermostable ferredoxin from Clostridium thermoaceticum.

    abstract::The complete amino acid sequence of the 4Fe-4S ferredoxin from the thermophilic bacterium Clostridium thermoaceticum has been determined. The protein is extremely thermostable and is the only known clostridial ferredoxin to contain a single [4Fe-4S] cluster. The sequence totals 63 residues and includes the first trypt...


    pub_type: 杂志文章


    authors: Elliott JI,Yang SS,Ljungdahl LG,Travis J,Reilly CF

    更新日期:1982-07-06 00:00:00

  • Dimerization specificity of adult and neonatal chicken skeletal muscle myosin heavy chain rods.

    abstract::The dimerization specificity of the recombinantly expressed and purified rod domain of adult and neonatal chicken myosin heavy chain was analyzed using metal chelation chromatography. Our results indicate that full-length adult and neonatal rods preferentially formed homodimers when renatured from an equimolar mixture...


    pub_type: 杂志文章


    authors: Singh S,Bandman E

    更新日期:2006-04-18 00:00:00

  • Paramecium calmodulin mutants defective in ion channel regulation associate with melittin in the absence of calcium but require it for tertiary collapse.

    abstract::Calmodulin (CaM) is a small acidic protein essential to calcium-mediated signal transduction. Conformational change driven by calcium binding controls its selective activation of myriad target proteins. In most well characterized cases, both homologous domains of CaM interact with a target protein. However, physiologi...


    pub_type: 杂志文章


    authors: Sorensen BR,Eppel JT,Shea MA

    更新日期:2001-01-30 00:00:00

  • Energy landscape of the Michaelis complex of lactate dehydrogenase: relationship to catalytic mechanism.

    abstract::Lactate dehydrogenase (LDH) catalyzes the interconversion between pyruvate and lactate with nicotinamide adenine dinucleotide (NAD) as a cofactor. Using isotope-edited difference Fourier transform infrared spectroscopy on the "live" reaction mixture (LDH·NADH·pyruvate ⇌ LDH·NAD(+)·lactate) for the wild-type protein an...


    pub_type: 杂志文章


    authors: Peng HL,Deng H,Dyer RB,Callender R

    更新日期:2014-03-25 00:00:00

  • Fourier transforms infrared difference spectroscopy of secondary quinone acceptor photoreduction in proton transfer mutants of Rhodobacter sphaeroides.

    abstract::In order to investigate the changes of protonation or environment of carboxylic residues occurring upon photoreduction of the secondary quinone acceptor (QB) in the reaction center (RC) of the photosynthetic bacteria Rhodobacter sphaeroides 2.4.1., we have performed light-induced Fourier transform infrared (FTIR) spec...


    pub_type: 杂志文章


    authors: Nabedryk E,Breton J,Hienerwadel R,Fogel C,Mäntele W,Paddock ML,Okamura MY

    更新日期:1995-11-14 00:00:00

  • The tyrosine photophysics of a primase-derived peptide are sensitive to the peptide's zinc-bound state: proof that the bacterial primase hypothetical zinc finger sequence binds zinc.

    abstract::A 35-amino acid peptide corresponding to the putative "zinc finger" sequence of primase was prepared to study its zinc binding properties. When zinc was added to the peptide, it was found that the fluorescence quantum yield of the single tyrosine increased by 46% and the average lifetime by 34%. The binding stoichiome...


    pub_type: 杂志文章


    authors: Griep MA,Adkins BJ,Hromas D,Johnson S,Miller J

    更新日期:1997-01-21 00:00:00

  • Assembly of A beta amyloid protofibrils: an in vitro model for a possible early event in Alzheimer's disease.

    abstract::Amyloid fibrils comprising primarily the peptides A beta 40 and A beta 42 are a defining feature of the Alzheimer's disease (AD) brain, and convergent evidence suggests that the process of their formation plays a central role in the AD pathogenic pathway. Elucidation of fibril assembly is critical for the discovery of...


    pub_type: 杂志文章


    authors: Harper JD,Wong SS,Lieber CM,Lansbury PT Jr

    更新日期:1999-07-13 00:00:00

  • Deglycosylated human follitropin: characterization and effects on adenosine cyclic 3',5'-phosphate production in porcine granulosa cells.

    abstract::Chemical deglycosylation of gonadotropins with hydrogen fluoride (HF) has facilitated the investigation of the structure-function relationship of the individual peptide and oligosaccharide moieties in the mechanism of hormone action. These studies have dealt almost exclusively with lutropin or human choriogonadotropin...


    pub_type: 杂志文章


    authors: Calvo FO,Keutmann HT,Bergert ER,Ryan RJ

    更新日期:1986-07-01 00:00:00

  • Specific proteolysis of human plasminogen by a 24 kDa endopeptidase from a novel Chryseobacterium Sp.

    abstract::A novel single polypeptide endopeptidase of 24 kDa (24k-endopeptidase) was purified with a yield of 300-400 microg/L from conditioned medium of a bacterial strain which was identified as a new species in the genus Chryseobacterium Sp. on the basis of its 16S rDNA sequence and DNA:DNA hybridizations. The NH(2)-terminal...


    pub_type: 杂志文章


    authors: Lijnen HR,Van Hoef B,Ugwu F,Collen D,Roelants I

    更新日期:2000-01-18 00:00:00

  • Composition and structure of zinc-deficient Euglena gracilis chromatin.

    abstract::The histone content of zinc-deficient (-Zn) Euglena gracilis decreases while, concomitantly, DNA content increases and the transcription rate is reduced markedly [Mazus, B., Falchuk, K. H., & Vallee, B. L. (1983) Biochemistry (in press); Falchuk, K. H., Fawcett, D. W., & Vallee, B. L. (1975) J. Cell Sci. 17, 57-78]. T...


    pub_type: 杂志文章


    authors: Stankiewicz AJ,Falchuk KH,Vallee BL

    更新日期:1983-10-25 00:00:00

  • The limits of promiscuity: isoform-specific dimerization of filamins.

    abstract::Filamins are a family of actin cross-linking proteins that are primarily localized in the cortical cytoplasm of all mammalian cells. Until now, three major isoforms (filamins a, b, and c) have been identified, that were shown to be differentially expressed and/or localized in different tissues. An amino-terminal doubl...


    pub_type: 杂志文章


    authors: Himmel M,Van Der Ven PF,Stöcklein W,Fürst DO

    更新日期:2003-01-21 00:00:00

  • Methylselenocysteine treatment leads to diselenide formation in human cancer cells: evidence from X-ray absorption spectroscopy studies.

    abstract::The selenoamino acids methylselenocysteine (MeSeCys) and selenomethionine (SeMet) have disparate efficacies as anticancer agents. Herein, we use X-ray absorption spectroscopy to determine the chemical form of selenium in human neuroblastoma cells. Cells treated with MeSeCys contain a significant diselenide component, ...


    pub_type: 杂志文章


    authors: Weekley CM,Aitken JB,Musgrave IF,Harris HH

    更新日期:2012-01-24 00:00:00