Stimulation of transcript elongation requires both the zinc finger and RNA polymerase II binding domains of human TFIIS.

abstract：:The binding of bacteriophage T4 DNA polymerase (T4 pol) to primer-template DNA with 2-aminopurine (2AP) located at the primer terminus results in the formation of a hyperfluorescent 2AP state. Changes in this hyperfluorescent state were utilized to investigate the fractional concentration of primer-templates bound at ...

journal_title：Biochemistry

authors： Beechem JM,Otto MR,Bloom LB,Eritja R,Reha-Krantz LJ,Goodman MF

更新日期：1998-07-14 00:00:00

abstract：:Horseradish peroxidase (HRP) was oxidized by IrCl6(2-) to a mixture of compounds I and II, the rate of oxidation and the ratio of the mixture being greatly affected by pH (Hayashi & Yamazaki, 1979). Oxidation of HRP by IrCl6(2-) in the presence of fluoride was significantly accelerated. This resulted in the formation ...

journal_title：Biochemistry

authors： Farhangrazi ZS,Sinclair R,Powers L,Yamazaki I

更新日期：1995-11-21 00:00:00

abstract：:The role of acetylation on the conformation and association state of the inner core histone octamer isolated from HeLa cells was examined. Preparation of suitable quantities of pure acetylated and control inner core histones from HeLa cells required the development of a new preparative procedure. The results from size...

journal_title：Biochemistry

authors： Prevelige PE Jr,Fasman GD

更新日期：1987-05-19 00:00:00

abstract：:R67 dihydrofolate reductase (DHFR), which catalyzes the NADPH dependent reduction of dihydrofolate to tetrahydrofolate, belongs to a type II family of R-plasmid encoded DHFRs that confer resistance to the antibacterial drug trimethoprim. Crystal structure data reveals this enzyme is a homotetramer that possesses a sin...

journal_title：Biochemistry

authors： Hicks SN,Smiley RD,Hamilton JB,Howell EE

更新日期：2003-09-16 00:00:00

abstract：:HcbA1 is a unique flavoenzyme that catalyzes the first step in the bacterial hexachlorobenzene catabolic pathway. Here we report in vitro reconstitution of the HcbA1-catalyzed reaction. Detailed mechanistic studies provide evidence for nucleophilic aromatic substitution and flavin-N5-oxide formation. ...

journal_title：Biochemistry

authors： Adak S,Begley TP

更新日期：2019-03-05 00:00:00

abstract：:Familial British dementia (FBD) is a rare neurodegenerative disorder and shares features with Alzheimer's disease, including amyloid plaque deposits, neurofibrillary tangles, neuronal loss, and progressive dementia. Immunohistochemical and biochemical analysis of plaques and vascular amyloid of FBD brains revealed tha...

journal_title：Biochemistry

authors： El-Agnaf OM,Sheridan JM,Sidera C,Siligardi G,Hussain R,Haris PI,Austen BM

更新日期：2001-03-27 00:00:00

abstract：:The photoreceptor protein rhodopsin has been reconstituted with a single phospholipid species, dimyristoylphosphatidylcholine, at a range of different lipid/protein ratios, and the exchange rate at the lipid-protein interface has been determined from the electron spin resonance spectra of spin-labeled phosphatidylchol...

journal_title：Biochemistry

authors： Ryba NJ,Horváth LI,Watts A,Marsh D

更新日期：1987-06-02 00:00:00

abstract：:Control of protein phosphatases is now understood to depend on binding to a variety of regulatory or targeting subunits to form holoenzymes with restricted localization and substrate specificity. In addition, the catalytic subunits of both type-1 and type-2 phosphatases bind specific inhibitor proteins. Here, we repor...

journal_title：Biochemistry

authors： Eto M,Karginov A,Brautigan DL

更新日期：1999-12-21 00:00:00

abstract：:The BARD1 N-terminal RING domain binds BRCA1 while the BARD1 C-terminal ankyrin and tandem BRCT repeat domains bind CstF-50 to modulate mRNA processing and RNAP II stability in response to DNA damage. Here we characterize the BARD1 structural biochemistry responsible for CstF-50 binding. The crystal structure of the B...

journal_title：Biochemistry

authors： Edwards RA,Lee MS,Tsutakawa SE,Williams RS,Nazeer I,Kleiman FE,Tainer JA,Glover JN

更新日期：2008-11-04 00:00:00

abstract：:The full three-dimensional structure of the catalytic domain of human stromelysin-1 (SCD) complexed to a novel and potent, nonpeptidic inhibitor has been determined by nuclear magnetic resonance spectroscopy (NMR). To accurately mimic assay conditions, the structure was obtained in Tris buffer at pH 6.8 and without th...

journal_title：Biochemistry

authors： Li YC,Zhang X,Melton R,Ganu V,Gonnella NC

更新日期：1998-10-06 00:00:00

abstract：:The Rieske protein from Thermus thermophilus (TtRp) and a truncated version of the protein (truncTtRp), produced to achieve a low-pH crystallization condition, have been characterized using UV-visible and circular dichroism spectroscopies. TtRp and truncTtRp undergo a change in the UV-visible spectra with increasing p...

journal_title：Biochemistry

authors： Konkle ME,Muellner SK,Schwander AL,Dicus MM,Pokhrel R,Britt RD,Taylor AB,Hunsicker-Wang LM

更新日期：2009-10-20 00:00:00

abstract：:When Pseudomonas putida ATCC 39167 and plant-deleterious Pseudomonas fluorescens A225 were grown in an iron-deficient culture medium, they each produced two different novel yellow-green fluorescent pseudobactins: P39167-I, II and PA225-I, II. Pseudobactin P39167-I has a molecular formula of C46H65O23N13 and is monoani...

journal_title：Biochemistry

authors： Khalil-Rizvi S,Toth SI,van der Helm D,Vidavsky I,Gross ML

更新日期：1997-04-08 00:00:00

abstract：:Thioredoxin reductase (TrxR) catalyzes the reduction of thioredoxin (Trx) by NADPH. A unique gene organization of TrxR and Trx has been found in Mycobacterium leprae, where TrxR and Trx are encoded by a single gene and, therefore, are expressed as a fusion protein (MlTrxR-Trx). This fusion enzyme is able to catalyze t...

journal_title：Biochemistry

authors： Wang PF,Marcinkeviciene J,Williams CH Jr,Blanchard JS

更新日期：1998-11-17 00:00:00

abstract：:A gentle and rapid isolation procedure is described yielding fractions containing better than 95% pure OmpF porin of Escherichia coli BE with different amounts of bound lipopolysaccharide (LPS). The procedure employs continuous free-flow electrophoresis (FFE) in the presence of detergent above its critical micelle con...

journal_title：Biochemistry

authors： Holzenburg A,Engel A,Kessler R,Manz HJ,Lustig A,Aebi U

更新日期：1989-05-16 00:00:00

abstract：:Calcium-saturated calmodulin (CaM) binds and influences the activity of a varied collection of target proteins in most cells. This promiscuity underlies the role of CaM as a shared participant in calcium-dependent signal transduction pathways but imposes a handicap on popular CaM-based calcium biosensors, which displa...

journal_title：Biochemistry

authors： Green DF,Dennis AT,Fam PS,Tidor B,Jasanoff A

更新日期：2006-10-17 00:00:00

abstract：:The trans-aconitate methyltransferase from the bacterium Escherichia coli catalyzes the monomethyl esterification of trans-aconitate and related compounds. Using two-dimensional (1)H/(13)C nuclear magnetic resonance spectroscopy, we show that the methylation is specific to one of the three carboxyl groups and further ...

journal_title：Biochemistry

authors： Cai H,Strouse J,Dumlao D,Jung ME,Clarke S

更新日期：2001-02-20 00:00:00

abstract：:We previously showed that a bulky ring-strained cycloalkyne possessing a rhodamine fluorophore directly reacts (via strain-promoted click chemistry) with the azido group incorporated (via ligand-directed tosyl chemistry) into Asp160 in the 49 kDa subunit of complex I in bovine heart submitochondrial particles [Masuya,...

journal_title：Biochemistry

authors： Masuya T,Murai M,Ito T,Aburaya S,Aoki W,Miyoshi H

更新日期：2017-08-15 00:00:00

abstract：:The interfacial interactions of cholesterol with sphingomyelins (SMs) containing various homogeneous acyl chains have been investigated by Langmuir film balance approaches. Low in-plane elasticity among the packed lipids was identified as an important physical feature of the cholesterol-sphingomyelin liquid-ordered ph...

journal_title：Biochemistry

authors： Li XM,Momsen MM,Smaby JM,Brockman HL,Brown RE

更新日期：2001-05-22 00:00:00

abstract：:Two glutathione S-transferase (GST) activities have been identified and purified from etiolated corn tissue. The first, designated GST I enzyme, is constitutively present in corn tissue, and the second, designated GST II enzyme, is present only in tissue which has been treated with chemical antidotes which protect cor...

journal_title：Biochemistry

authors： Mozer TJ,Tiemeier DC,Jaworski EG

更新日期：1983-03-01 00:00:00

abstract：:The growth and shortening of microtubules in guanosine triphosphate-(GTP-) mediated dynamic instability has previously been observed to occur at rates which are remarkably variable (Gildersleeve et al., 1992, Chrétien et al., 1995). Neighboring microtubules observed simultaneously can grow or shorten at different rate...

journal_title：Biochemistry

authors： Dye RB,Williams RC Jr

更新日期：1996-11-12 00:00:00

abstract：:Conformational factors that predicate selectivity for valine or isoleucine binding to IlvN leading to the regulation of aceto hydroxy acid synthase I (AHAS I) of Escherichia coli have been determined for the first time from high-resolution (1.9-2.43 Å) crystal structures of IlvN·Val and IlvN·Ile complexes. The valine ...

journal_title：Biochemistry

authors： Bansal A,Karanth NM,Demeler B,Schindelin H,Sarma SP

更新日期：2019-04-16 00:00:00

abstract：:Anastellin is a small recombinant fragment derived from the extracellular matrix protein fibronectin; it comprises the first type III (FN3) domain without the two N-terminal β-strands. It inhibits angiogenesis, tumor growth, and metastasis in mouse models and requires endogenous fibronectin for its in vivo anti-angiog...

journal_title：Biochemistry

authors： Stine JM,Ahl GJH,Schlenker C,Rusnac DV,Briknarová K

更新日期：2017-09-05 00:00:00

abstract：:A methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase (MeTr) from Clostridium thermoaceticum catalyzes the transfer of the N5 methyl group from (6S)-methyltetrahydrofolate (CH3-H4folate) to the cobalt center of a corrinoid/iron-sulfur protein (C/Fe-SP). The methylcobamide product is the first in a s...

journal_title：Biochemistry

authors： Zhao S,Roberts DL,Ragsdale SW

更新日期：1995-11-21 00:00:00

abstract：:The initiation of Escherichia coli chromosomal replication by DnaA protein is strongly influenced by the tight binding of the nucleotides ATP and ADP. Anionic phospholipids in a fluid bilayer promote the conversion of inactive ADP-DnaA protein to replicatively active ATP-DnaA protein in vitro, and thus likely play a k...

journal_title：Biochemistry

authors： Kitchen JL,Li Z,Crooke E

更新日期：1999-05-11 00:00:00

abstract：:Light-driven outward sodium-pumping rhodopsin (NaR) was recently found in marine bacteria. Krokinobacter eikastus rhodopsin 2 (KR2) actively transports sodium and lithium ions in NaCl and LiCl, respectively, while it pumps protons in KCl. NaR has a conserved NDQ (N112, D116, and Q123 in KR2) motif, and previous studie...

journal_title：Biochemistry

authors： Abe-Yoshizumi R,Inoue K,Kato HE,Nureki O,Kandori H

更新日期：2016-10-18 00:00:00

abstract：:The solution secondary structure of the highly active Y55F/Y88F "Tyr-14-only" mutant of delta 5-3-ketosteroid isomerase complexed with 19-nortestosterone hemisuccinate has been shown to consist of three helices, a six-stranded mixed beta-sheet, and five turns. The steroid binds near the general acid, Tyr-14, on helix ...

journal_title：Biochemistry

authors： Zhao Q,Abeygunawardana C,Gittis AG,Mildvan AS

更新日期：1997-12-02 00:00:00

abstract：:In efforts to understand the mechanisms of many nucleic acid enzymes, the first site-directed mutations are made at conserved acidic active residues. Almost without exception, the low or null activities of the resulting variants are attributed to the importance of the acidic residue(s) to the ligation of required meta...

journal_title：Biochemistry

authors： Dupureur CM,Conlan LH

更新日期：2000-09-05 00:00:00

abstract：:Safe and effective algaecides are needed to control agriculturally and environmentally significant algal species. Four series (6, 10, 17, and 21) of 29 novel 4-aminopyrimidine derivatives were rationally designed and synthesized. A part of 10, 17, and 21 displayed potent inhibition of Escherichia coli pyruvate dehydro...

journal_title：Biochemistry

authors： Zhou Y,Feng J,He H,Hou L,Jiang W,Xie D,Feng L,Cai M,Peng H

更新日期：2017-12-12 00:00:00

abstract：:Extended-spectrum β-lactamases (ESBLs) pose a threat to public health because of their ability to confer resistance to extended-spectrum cephalosporins such as cefotaxime. The CTX-M β-lactamases are the most widespread ESBL enzymes among antibiotic resistant bacteria. Many of the active site residues are conserved bet...

journal_title：Biochemistry

authors： Adamski CJ,Cardenas AM,Brown NG,Horton LB,Sankaran B,Prasad BV,Gilbert HF,Palzkill T

更新日期：2015-01-20 00:00:00

abstract：:Neuronal specification is regulated by the activity of transcription factors containing the basic helix-loop-helix motif (bHLH); these regulating proteins include, among others, the neurogenin (Ngn) family, related to the atonal family of genes. Neurogenin 1 (NGN1) is a 237-residue protein that contains a bHLH domain ...

journal_title：Biochemistry

authors： Aguado-Llera D,Goormaghtigh E,de Geest N,Quan XJ,Prieto A,Hassan BA,Gómez J,Neira JL

更新日期：2010-03-02 00:00:00