Abstract：
:In spite of a nuclear targeting sequence, a substantial amount of dual-specificity tyrosine phosphorylation-regulated kinase (Dyrk1A) is located within the cytoplasm of neurons. Analysis of fractionated rat brains revealed that the majority of Dyrk1A was in the postnuclear precipitate. The kinase in this fraction was resistant to high salt and Triton X-100 extraction at pH 6.5. Hypothesizing that Dyrk1A binds tightly with cell constituents, we searched for Dyrk1A binding proteins in the Triton X-100-insoluble fraction extracted with urea and fractionated by column chromatography. An overlay assay using the recombinant kinase revealed that multiple proteins are capable of binding to Dyrk1A. Among them, we identified clathrin heavy chain and dynamin 1 as potential candidates. An overlay assay using purified and partially purified proteins showed the binding of Dyrk1A with both proteins. Under native conditions, Dyrk1A precipitated with newly formed clathrin cages and with dynamin via the GST-amphiphysin SH3 domain. We also identified another endocytic protein, endophilin 1, as an additional Dyrk1A binding protein. We then tested whether the clathrin-coated vesicle (CCV)-associated proteins could be phosphorylated by Dyrk1A. Multiple proteins apparently distinctive from the known substrates were phosphorylated in the brain CCV. Our findings suggest a role for Dyrk1A in controlling synaptic vesicle recycling processes.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Murakami N,Bolton D,Hwang YWdoi
10.1021/bi9010557subject
Has Abstractpub_date
2009-10-06 00:00:00pages
9297-305issue
39eissn
0006-2960issn
1520-4995journal_volume
48pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The dynamics of the single-stranded, helix--coil transition of poly(adenylic acid) have been investigated by using the Raman laser temperature jump technique. The driving forces of this conformational transition have been probed by varying the cosolvent in mixed aqueous solutions. The rate of helix formation correlate...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00549a025
更新日期：1980-04-15 00:00:00
abstract：:Putative domain--domain interactions of the monomeric bifunctional enzyme indoleglycerol phosphate synthase:phosphoribosyl anthranilate isomerase from Escherichia coli were probed by separating the domains on the gene level and expressing them as monofunctional proteins. The engineered monofunctional enzymes were foun...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00016a013
更新日期：1995-04-25 00:00:00
abstract：:Nitroalkane oxidase (NAO) from Fusarium oxysporum catalyzes the oxidation of neutral nitroalkanes to the corresponding aldehydes or ketones with the production of H(2)O(2) and nitrite. The flavoenzyme is a new member of the acyl-CoA dehydrogenase (ACAD) family, but it does not react with acyl-CoA substrates. We presen...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi051966w
更新日期：2006-01-31 00:00:00
abstract：:A fluorescence quenching method was developed for determining partition coefficients and diffusional rates of small molecules in cell membranes. This method involves quenching the fluorescence of carbazole-labeled membranes by hydrophobic molecules that partition into membranes. Cell membrane phospholipids of mouse LM...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00316a021
更新日期：1984-10-09 00:00:00
abstract：:The stereochemistry of proton transfer in the isomerization of [4 beta-2H]-5-androstene-3,17- dione (1d) to 4-androstene-3,17-dione (3) catalyzed by 3-oxo-delta 5-steroid isomerase (KSI) has been reinvestigated. In H2O, approximately 65% of the label is retained in the product (3); of this, one-third is at C-4 and two...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi953025x
更新日期：1996-05-21 00:00:00
abstract：:The biophysical analysis of multidomain proteins often is difficult because of overlapping signals from the individual domains. Previously, the fluorescent unnatural amino acid p-cyanophenylalanine has been used to study the folding of small single-domain proteins. Here we extend its use to a two-domain protein to sel...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00898
更新日期：2016-12-13 00:00:00
abstract：:A peptide corresponding to a portion of the amphipathic alpha-helical region of CTP:phosphocholine cytidylyltransferase was synthesized. This region of the enzyme was proposed to be the membrane-binding domain [Kalmar, G.B., Kay, R.J., Lachance, A., Aebersold, R., & Cornell, R.B. (1990) Proc. Natl. Acad. Sci. U.S.A. 8...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00180a029
更新日期：1994-04-12 00:00:00
abstract：:Inhibition of gamma-aminobutyric acid aminotransferase (GABA-AT) increases the concentration of GABA, an inhibitory neurotransmitter in human brain, which could have therapeutic applications for a variety of neurological diseases, including epilepsy. On the basis of studies of several previously synthesized conformati...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi061592m
更新日期：2006-12-05 00:00:00
abstract：:The complete amino acid sequence of the major component myoglobin from the dwarf sperm whale, Kogia simus, was determined by specific cleavage of the protein to obtain large peptides which are readily degraded by the automatic sequenator. Three easily separable peptides were obtained by cleaving the protein at its two...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00624a010
更新日期：1977-03-08 00:00:00
abstract：:X-ray crystallographic data have implicated Arg-292 as the residue responsible for the preferred side-chain substrate specificity of aspartate aminotransferase. It forms a salt bridge with the beta or gamma carboxylate group of the substrate [Kirsch, J. F., Eichele, G., Ford, G. C., Vincent, M. G., Jansonius, J. N., G...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00412a052
更新日期：1988-06-14 00:00:00
abstract：:The virally encoded integrase protein carries out retroviral integration, which requires specific interactions with the two ends of the viral DNA, and also with host DNA that is the target of integration. We attached a photo-cross-linking agent to specific viral and target DNA sites to identify regions of the integras...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972949c
更新日期：1998-05-12 00:00:00
abstract：:Cytochrome c binds to cardiolipin (CL) on the inner mitochondrial membrane during the initial stages of apoptosis where it oxidizes CL, promoting its release into the cytoplasm where it initiates apoptosis. Previous work has identified interaction sites on cytochrome c involved in the cytochrome c-CL interaction. The ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b00704
更新日期：2018-10-02 00:00:00
abstract：:Oligonucleotide-directed triple helix formation normally requires a long tract of oligopyrimidine.oligopurine sequence. This limitation can be partially overcome by alternate-strand triple helix (or switch triple helix) formation which enables recognition of alternating oligopurine/oligopyrimidine sequences. The prese...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi980618+
更新日期：1998-09-22 00:00:00
abstract：:Product distributions for the reaction of glycolaldehyde labeled with carbon-13 at the carbonyl carbon ([1-(13)C]-GA) catalyzed by triosephosphate isomerase (TIM) in D(2)O at pD 7.0 in the presence of phosphite dianion and in its absence were determined by (1)H NMR spectroscopy. We observe three products for the relat...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi900636c
更新日期：2009-06-23 00:00:00
abstract：:We have isolated from rabbit liver three cDNA clones of 1400-1800 base pairs that hybridize selectively to RNA from animals treated with phenobarbital. The nucleotide sequences of the cDNAs have been determined. In the protein coding region the nucleotide sequences of two of the cDNAs are 88% homologous, and the third...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00297a005
更新日期：1984-01-17 00:00:00
abstract：:The oxidation pathway of chlorophyll Z (ChlZ) in photosystem II (PSII) at cryogenic temperatures was studied by means of light-induced Fourier transform infrared (FTIR) difference spectroscopy. To examine the involvement of redox-active beta-carotene (Car) in the pathway, two Car molecules in Mn-depleted PSII membrane...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi052346y
更新日期：2006-02-14 00:00:00
abstract：:The oxidative stress products malondialdehyde and base propenal react with DNA bases forming the adduction products 3-(2'-deoxy-β-D-erythro-pentofuranosyl)pyrimido[1,2-a]purin-10(3H)-one (M1dG) and N(6)-(oxypropenyl)-2'-deoxyadenosine (OPdA). M1dG is mutagenic in vivo and miscodes in vitro, but little work has been do...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi401103k
更新日期：2013-12-03 00:00:00
abstract：:Structural changes during the photochemical reactions of unhydrated air-dried films of bovine rhodopsin in rod outer segments were examined by visible and Fourier transform infrared (FTIR) spectroscopy at 200, 240, and 280 K. These films exhibited conversion from a lumirhodopsin state to a metarhodopsin I state with a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00051a025
更新日期：1995-12-26 00:00:00
abstract：:The fluorescent nucleotide analogue of ADP, 1,N6-ethenoadenosine diphosphate (epsilon ADP), has been used to probe the active site of myosin subfragment 1 (SF1). The Mg complex of ADP was shown to be trapped stoichiometrically at the active site by a variety of thiol cross-linking agents having sulfur to sulfur spanni...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00312a029
更新日期：1984-08-14 00:00:00
abstract：:The nuclear transport of the chromatin remodeling protein nucleoplasmin and chromatin building histones is mediated by importins. Nucleoplasmin (NP) contains a classical bipartite nuclear localization signal (NLS) that is recognized by the importin α/β heterodimer, while histones present multiple NLS-like motifs that ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi2008867
更新日期：2011-08-23 00:00:00
abstract：:We have investigated the role of the labile terminal domains of the core histones on the stability of the subunits of the protein core of the nucleosome by studying the thermodynamic behavior of the products of limited trypsin digestion of these subunits. The thermal stabilities of the truncated H2A-H2B dimer and the ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0110140
更新日期：2001-10-30 00:00:00
abstract：:The formation and role of arachidonic acid (AA) and its metabolites during gonadotropin releasing hormone- (GnRH-) induced gonadotropin secretion were investigated in primary cultures of rat pituitary cells. Prelabeled cells ([3H]AA) responded to GnRH challenge with increased formation (about 2-fold) of the leukotrien...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00139a004
更新日期：1992-06-23 00:00:00
abstract：:Large conductance Ca(2+)-activated K(+) channels (BK(Ca)) contain an intracellular binding site for bovine pancreatic trypsin inhibitor (BPTI), a well-known inhibitor of various serine proteinase (SerP) enzymes. To investigate the structural basis of this interaction, we examined the activity of 11 BPTI mutants using ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi992140v
更新日期：2000-02-29 00:00:00
abstract：:In reticulocytes, a single ribosomal protein, S13, has been shown to be phosphorylated by the cAMP-regulated protein kinases. The 40S ribosomal subunits were phosphorylated in vitro with [gamma-32P]ATP to facilitate the identification of S13 during the two-step purification procedure. Total ribosomal protein from the ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00604a003
更新日期：1978-05-30 00:00:00
abstract：:The folding of the peptide chain of the bovine heart oxoglutarate carrier in the inner mitochondrial membrane and in the membrane of reconstituted proteoliposomes has been investigated by enzymatic and immunochemical approaches using proteinase K and polyclonal site-directed antibodies, respectively. Two peptides corr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00178a030
更新日期：1994-03-29 00:00:00
abstract：:Tat, an essential human immunodeficiency virus type 1 protein interacts with the transactivation response element (TAR) and stimulates transcription from the viral long-terminal repeat (LTR). Blockage of Tat-TAR interaction halts viral transcription and hence replication. We have found that polyamide nucleic acid (PNA...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi000708q
更新日期：2000-09-26 00:00:00
abstract：:Previous work has shown that calmodulin (CaM) is constitutively phosphorylated in rat liver, probably by casein kinase II [Quadroni, M., James, P., and Carafoli, E. (1994) J. Biol. Chem. 269, 16116-16122]. A procedure is now described for the isolation of the phosphorylated forms of calmodulin (PCaM) free from CaM, si...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972930+
更新日期：1998-05-05 00:00:00
abstract：:Modification of the bacterial cell wall heteropolymer peptidoglycan by addition of an acetyl group to the C-6 hydroxyl group of N-acetylmuramoyl residues is known to inhibit the activity of muramidases (lysozymes) of innate immune systems. The O-acetylation of peptidoglycan also precludes the action of intrinsic lytic...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi051679s
更新日期：2006-01-24 00:00:00
abstract：:We have detected at least four forms of DNA-dependent ATPase in mouse FM3A cell extracts [Tawaragi, Y., Enomoto, T., Watanabe, Y., Hanaoka, F., & Yamada, M. (1984) Biochemistry 23, 529-533]. The purified fraction of one of the four forms, ATPase B, has been shown to have DNA helicase activity by using a DNA substrate ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00384a038
更新日期：1987-05-19 00:00:00
abstract：:Cytochrome b(561) in adrenal chromaffin vesicle membranes conveys electron equivalents from extravesicular ascorbate to the intravesicular monodehydroascorbate radical. We conducted a stopped-flow study on the reaction of ascorbate with purified cytochrome b(561) in the detergent-solubilized state for the first time. ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0267588
更新日期：2003-07-15 00:00:00
