Identification of the serine residue phosphorylated by protein kinase C in vertebrate nonmuscle myosin heavy chains.


:Two-dimensional mapping of the tryptic phosphopeptides generated following in vitro protein kinase C phosphorylation of the myosin heavy chain isolated from human platelets and chicken intestinal epithelial cells shows a single radioactive peptide. These peptides were found to comigrate, suggesting that they were identical, and amino acid sequence analysis of the human platelet tryptic peptide yielded the sequence -Glu-Val-Ser-Ser(PO4)-Leu-Lys-. Inspection of the amino acid sequence for the chicken intestinal epithelial cell myosin heavy chain (196 kDa) derived from cDNA cloning showed that this peptide was identical with a tryptic peptide present near the carboxyl terminal of the predicted alpha-helix of the myosin rod. Although other vertebrate nonmuscle myosin heavy chains retain neighboring amino acid sequences as well as the serine residue phosphorylated by protein kinase C, this residue is notably absent in all vertebrate smooth muscle myosin heavy chains (both 204 and 200 kDa) sequenced to date.






Conti MA,Sellers JR,Adelstein RS,Elzinga M




Has Abstract


1991-01-29 00:00:00












  • Selective cytotoxicity of rhodium metalloinsertors in mismatch repair-deficient cells.

    abstract::Mismatches in DNA occur naturally during replication and as a result of endogenous DNA damaging agents, but the mismatch repair (MMR) pathway acts to correct mismatches before subsequent rounds of replication. Rhodium metalloinsertors bind to DNA mismatches with high affinity and specificity and represent a promising ...


    pub_type: 杂志文章


    authors: Ernst RJ,Komor AC,Barton JK

    更新日期:2011-12-20 00:00:00

  • Membrane trafficking of aquaporin 1 is mediated by protein kinase C via microtubules and regulated by tonicity.

    abstract::It is well-known that the rapid flow of water into and out of cells is controlled by membrane proteins called aquaporins (AQPs). However, the mechanisms that allow cells to quickly respond to a changing osmotic environment are less well established. Using GFP-AQP fusion proteins expressed in HEK293 cells, we demonstra...


    pub_type: 杂志文章


    authors: Conner MT,Conner AC,Brown JE,Bill RM

    更新日期:2010-02-09 00:00:00

  • Bidirectional Transformation of a Metamorphic Protein between the Water-Soluble and Transmembrane Native States.

    abstract::The bidirectional transformation of a protein between its native water-soluble and integral transmembrane conformations is demonstrated for FraC, a hemolytic protein of the family of pore-forming toxins. In the presence of biological membranes, the water-soluble conformation of FraC undergoes a remarkable structural r...


    pub_type: 杂志文章


    authors: Tanaka K,Caaveiro JM,Tsumoto K

    更新日期:2015-11-24 00:00:00

  • New protein cross-linking reagents that are cleaved by mild acid.

    abstract::New homo- and heterobifunctional cross-linking reagents have been synthesized. These reagents are based on ortho ester, acetal, and ketal functionalities that undergo acid-catalyzed dissociation but are base stable. The protein-reactive group in all the homobifunctional reagents is a maleimide group; the heterobifunct...


    pub_type: 杂志文章


    authors: Srinivasachar K,Neville DM Jr

    更新日期:1989-03-21 00:00:00

  • Steric hindrance as a basis for structure-based design of selective inhibitors of protein-tyrosine phosphatases.

    abstract::Utilizing structure-based design, we have previously demonstrated that it is possible to obtain selective inhibitors of protein-tyrosine phosphatase 1B (PTP1B). A basic nitrogen was introduced into a general PTP inhibitor to form a salt bridge to Asp48 in PTP1B and simultaneously cause repulsion in PTPs containing an ...


    pub_type: 杂志文章


    authors: Iversen LF,Andersen HS,Møller KB,Olsen OH,Peters GH,Branner S,Mortensen SB,Hansen TK,Lau J,Ge Y,Holsworth DD,Newman MJ,Hundahl Møller NP

    更新日期:2001-12-11 00:00:00

  • Effects of mutations in M4 of the gastric H+,K+-ATPase on inhibition kinetics of SCH28080.

    abstract::The effects of site-directed mutagenesis were used to explore the role of residues in M4 on the apparent Ki of a selective, K+-competitive inhibitor of the gastric H+,K+ ATPase, SCH28080. A double transfection expression system is described, utilizing HEK293 cells and separate plasmids encoding the alpha and beta subu...


    pub_type: 杂志文章


    authors: Munson KB,Lambrecht N,Sachs G

    更新日期:2000-03-21 00:00:00

  • Dynamic structure of a highly ordered beta-sheet molten globule: multiple conformations with a stable core.

    abstract::The structure of [14-38]Abu, a variant of bovine pancreatic trypsin inhibitor (BPTI) with only the 14-38 disulfide bridge intact, has been analyzed by two-dimensional 1H and 1H-15N NMR. Except for the 18-24, 29-35 antiparallel beta-sheet, residues in all regions of the molecule give two exchange cross peaks for each 1...


    pub_type: 杂志文章


    authors: Barbar E,Barany G,Woodward C

    更新日期:1995-09-12 00:00:00

  • The amino- and carboxyl-terminal fragments of the Bacillus thuringensis Cyt1Aa toxin have differential roles in toxin oligomerization and pore formation.

    abstract::The Cyt toxins produced by the bacteria Bacillus thuringiensis show insecticidal activity against some insects, mainly dipteran larvae, being able to kill mosquitoes and black flies. However, they also possess a general cytolytic activity in vitro, showing hemolytic activity in red blood cells. These proteins are comp...


    pub_type: 杂志文章


    authors: Rodriguez-Almazan C,Ruiz de Escudero I,Cantón PE,Muñoz-Garay C,Pérez C,Gill SS,Soberón M,Bravo A

    更新日期:2011-01-25 00:00:00

  • Structure of bovine adrenal dopamine beta-monooxygenase, as deduced from cDNA and protein sequencing: evidence that the membrane-bound form of the enzyme is anchored by an uncleaved signal peptide.

    abstract::A full-length cDNA for dopamine beta-monooxygenase (D beta M) from bovine adrenal glands has been cloned and sequenced. The soluble and membrane-derived forms of D beta M have also been sequenced from their N-termini. While the observed sequences for the soluble protein correspond to those previously reported [Joh, T....


    pub_type: 杂志文章


    authors: Taljanidisz J,Stewart L,Smith AJ,Klinman JP

    更新日期:1989-12-26 00:00:00

  • Regioselectivity in the homolytic cleavage of S-adenosylmethionine.

    abstract::The observed regioselectivity of the homolytic cleavage of S-adenosylmethionine (SAM) by the radical SAM enzymes is modeled by free radical displacement reactions at sulfoxide centers. These displacements are also regioselective, in direct consequence of the reaction mechanism. The selectivity in the radical SAM react...


    pub_type: 杂志文章


    authors: Kampmeier JA

    更新日期:2010-12-28 00:00:00

  • Synthesis and spectroscopic studies of the aminoglycoside (neomycin)--perylene conjugate binding to human telomeric DNA.

    abstract::Synthesis of a novel perylene-neomycin conjugate (3) and the properties of its binding to human telomeric G-quadruplex DNA, 5'-d[AG3(T2AG3)3] (4), are reported. Various spectroscopic techniques were employed to characterize the binding of conjugate 3 to 4. A competition dialysis assay revealed that 3 preferentially bi...


    pub_type: 杂志文章


    authors: Xue L,Ranjan N,Arya DP

    更新日期:2011-04-12 00:00:00

  • Refolding of amphioxus insulin-like peptide: implications of a bifurcating evolution of the different folding behavior of insulin and insulin-like growth factor 1.

    abstract::Insulin and insulin-like growth factor 1 (IGF-1) share high sequence homology, but their folding behaviors are significantly different: insulin folds into one unique thermodynamically controlled structure, while IGF-1 folds into two thermodynamically controlled disulfide isomers. However, the origin of their different...


    pub_type: 杂志文章


    authors: Wang S,Guo ZY,Shen L,Zhang YJ,Feng YM

    更新日期:2003-08-19 00:00:00

  • Evidence for the kinetic partitioning of polymerase activity on G-quadruplex DNA.

    abstract::We have investigated the action of the human DNA polymerase ε (hpol ε) and η (hpol η) catalytic cores on G-quadruplex (G4) DNA substrates derived from the promoter of the c-MYC proto-oncogene. The translesion enzyme hpol η exhibits a 6.2-fold preference for binding to G4 DNA over non-G4 DNA, while hpol ε binds both G4...


    pub_type: 杂志文章


    authors: Eddy S,Maddukuri L,Ketkar A,Zafar MK,Henninger EE,Pursell ZF,Eoff RL

    更新日期:2015-05-26 00:00:00

  • Genetic identification of an autoinhibitor in CDPK, a protein kinase with a calmodulin-like domain.

    abstract::CDPKs are a family of calcium (Ca2+)-dependent protein kinases which are defined by a carboxyl-terminal calmodulin-like domain. Mutational analysis indicates that the junction domain, which joins the kinase and calmodulin-like domains, contains an autoinhibitor. CDPK isoform AK1 from Arabidopsis was expressed in Esche...


    pub_type: 杂志文章


    authors: Harper JF,Huang JF,Lloyd SJ

    更新日期:1994-06-14 00:00:00

  • Differential scanning calorimetry of the unfolding of myosin subfragment 1, subfragment 2, and heavy meromyosin.

    abstract::The thermal unfolding of rabbit skeletal heavy meromyosin (HMM), myosin subfragment 1, and subfragment 2 has been studied by differential scanning calorimetry (DSC). Two distinct endotherms are observed in the DSC scan of heavy meromyosin. The first endotherm, with a Tm of 41 degrees C at pH 7.9 in 0.1 M KCl, is assig...


    pub_type: 杂志文章


    authors: Shriver JW,Kamath U

    更新日期:1990-03-13 00:00:00

  • A photolabile oligodeoxyribonucleotide probe of the peptidyltransferase center: identification of neighboring ribosomal components.

    abstract::In this work we report the synthesis of a radioactive, photolabile oligodeoxyribonucleotide probe and its exploitation in identifying 50S ribosomal subunit components neighboring its target site in 23S rRNA. The probe is complementary to 23S rRNA nucleotides 2497-2505, a single-stranded sequence that has been shown to...


    pub_type: 杂志文章


    authors: Muralikrishna P,Cooperman BS

    更新日期:1991-06-04 00:00:00

  • Electronic spectroscopy of cobalt angiotensin converting enzyme and its inhibitor complexes.

    abstract::Zinc, the catalytically essential metal of angiotensin converting enzyme (ACE), has been replaced by cobalt(II) to give an active, chromophoric enzyme that is spectroscopically responsive to inhibitor binding. Visible absorption spectroscopy and magnetic circular dichroic spectropolarimetry have been used to character...


    pub_type: 杂志文章


    authors: Bicknell R,Holmquist B,Lee FS,Martin MT,Riordan JF

    更新日期:1987-11-17 00:00:00

  • Crystal structure of the petal death protein from carnation flower.

    abstract::Expression of the PSR132 protein from Dianthus caryophyllus (carnation, clover pink) is induced in response to ethylene production associated with petal senescence, and thus the protein is named petal death protein (PDP). Recent work has established that despite the annotation of PDP in sequence databases as carboxyph...


    pub_type: 杂志文章


    authors: Teplyakov A,Liu S,Lu Z,Howard A,Dunaway-Mariano D,Herzberg O

    更新日期:2005-12-20 00:00:00

  • Multivector fluorescence analysis of the xpt guanine riboswitch aptamer domain and the conformational role of guanine.

    abstract::Purine riboswitches are RNA regulatory elements that control purine metabolism in response to intracellular concentrations of the purine ligands. Conformational changes of the guanine riboswitch aptamer domain induced by guanine binding lead to transcriptional regulation of genes involved in guanine biosynthesis. The ...


    pub_type: 杂志文章


    authors: Brenner MD,Scanlan MS,Nahas MK,Ha T,Silverman SK

    更新日期:2010-03-02 00:00:00

  • DNA damage by the enediyne C-1027 results in the inhibition of DNA replication by loss of replication protein A function and activation of DNA-dependent protein kinase.

    abstract::Treatment of cells with the enediyne C-1027 is highly efficient at inducing single- and double-strand DNA breaks. This agent is highly cytotoxic when used at picomolar levels over a period of days. For this study, C-1027 has been used at higher levels for a much shorter time period to look at early cellular responses ...


    pub_type: 杂志文章


    authors: Liu JS,Kuo SR,Yin X,Beerman TA,Melendy T

    更新日期:2001-12-04 00:00:00

  • Influence of the extent of branching on solution conformations of complex oligosaccharides: a molecular dynamics and NMR study of a penta-antennary "bisected" N-glycan.

    abstract::The solution conformation of an agalactosyl penta-antennary "bisected" N-linked glycan from hen ovomucoid has been determined using a combination of 1H-NMR NOE measurements and restrained molecular dynamics (MD) simulations. The majority of glycosidic linkages exhibited restricted torsional fluctuations about the glob...


    pub_type: 杂志文章


    authors: Rutherford TJ,Neville DC,Homans SW

    更新日期:1995-10-31 00:00:00

  • Cadmium-113 nuclear magnetic resonance studies of cadmium-substituted derivatives of bovine superoxide dismutase.

    abstract::We have prepared the following cadmium-113-substituted derivatives of bovine superoxide dismutase and recorded the nuclear magnetic resonance (NMR) spectrum of the cadmium: 2Cd(II), in which Cd(II) is presumed to bind to the Zn(II) site and the copper site is unoccupied, and 2Cd(II)--2Cu(I), which is analogous to the ...


    pub_type: 杂志文章


    authors: Bailey DB,Ellis PD,Fee JA

    更新日期:1980-02-05 00:00:00

  • Redesign of the interior hydrophilic region of mitochondrial cytochrome c by site-directed mutagenesis.

    abstract::Heme propionate-7 in cytochrome c is an ionizable group located in a region of the protein that is inaccessible to bulk solvent. Electrostatic stabilization of this functional group appears to be achieved through interaction of heme propionate-7 with several amino acid residues that occur within hydrogen-bonding dista...


    pub_type: 杂志文章


    authors: Davies AM,Guillemette JG,Smith M,Greenwood C,Thurgood AG,Mauk AG,Moore GR

    更新日期:1993-05-25 00:00:00

  • Effects of guanidine hydrochloride on the proton inventory of proteins: implications on interpretations of protein stability.

    abstract::The DeltaG degrees (N)(-)(D) value obtained from extrapolation to zero denaturant concentration by the linear extrapolation method (LEM) is commonly interpreted to represent the Gibbs energy difference between native (N) and denatured (D) ensembles at the limit of zero denaturant concentration. For DeltaG degrees (N)(...


    pub_type: 杂志文章


    authors: Bolen DW,Yang M

    更新日期:2000-12-12 00:00:00

  • Effects of replacement of a double bond by a cyclopropane ring in phosphatidylethanolamines: a 2H NMR study of phase transitions and molecular organization.

    abstract::The thermotropic behavior and molecular properties of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) and 1-palmitoyl-2-dihydrosterculoyl-sn-glycero-3-phosphoethanolamine (PDSPE) have been investigated by 2H NMR spectroscopy using samples selectively labeled at the 5'-, 9'-, 10'-, and 16'-positions of the...


    pub_type: 杂志文章


    authors: Perly B,Smith IC,Jarrell HC

    更新日期:1985-02-12 00:00:00

  • Formation and rejoining of deoxyribonucleic acid double-strand breaks induced in isolated cell nuclei by antineoplastic intercalating agents.

    abstract::The biochemical characteristics of the formation and disappearance of intercalator-induced DNA double-strand breaks (DSB) were studied in nuclei from mouse leukemia L1210 cells by using filter elution methodology [Bradley, M. O., & Kohn, K.W. (1979) Nucleic Acids Res. 7, 793-804]. The three intercalators used were 4'-...


    pub_type: 杂志文章


    authors: Pommier Y,Schwartz RE,Kohn KW,Zwelling LA

    更新日期:1984-07-03 00:00:00

  • Three-dimensional structure of potato carboxypeptidase inhibitor in solution. A study using nuclear magnetic resonance, distance geometry, and restrained molecular dynamics.

    abstract::The solution conformation of potato carboxypeptidase inhibitor (CPI) has been investigated by 1H NMR spectroscopy. The spectrum is assigned in a sequential manner by using two-dimensional NMR techniques to identify through-bond and through-space (less than 5 A) connectivities. A set of 309 approximate interproton dist...


    pub_type: 杂志文章


    authors: Clore GM,Gronenborn AM,Nilges M,Ryan CA

    更新日期:1987-12-01 00:00:00

  • Analysis of substrate access to active sites in bacterial multicomponent monooxygenase hydroxylases: X-ray crystal structure of xenon-pressurized phenol hydroxylase from Pseudomonas sp. OX1.

    abstract::In all structurally characterized bacterial multicomponent monooxygenase (BMM) hydroxylase proteins, a series of hydrophobic cavities in the α-subunit trace a conserved path from the protein exterior to the carboxylate-bridged diiron active site. This study examines these cavities as a potential route for transport of...


    pub_type: 杂志文章


    authors: McCormick MS,Lippard SJ

    更新日期:2011-12-27 00:00:00

  • High-yield functional expression of human sodium/d-glucose cotransporter1 in Pichia pastoris and characterization of ligand-induced conformational changes as studied by tryptophan fluorescence.

    abstract::Studies on the structure-function relationship of transporters require the availability of sufficient amounts of the protein in a functional state. In this paper, we report the functional expression, purification, and reconstitution of the human sodium/d-glucose cotransporter1 (hSGLT1) in Pichia pastoris and ligand-in...


    pub_type: 杂志文章


    authors: Tyagi NK,Goyal P,Kumar A,Pandey D,Siess W,Kinne RK

    更新日期:2005-11-29 00:00:00

  • Thermodynamic Stability of DNA Duplexes Comprising the Simplest T → dU Substitutions.

    abstract::Members of the uracil-DNA glycosylase (UDG) enzyme family recognize and bind uracil, sequestering it within the binding site pocket and catalyzing the cleavage of the base from the deoxyribose, leaving an abasic site. The recognition and binding are passive and rely on innate dynamic motions of DNA wherein base pairs ...


    pub_type: 杂志文章


    authors: Carr CE,Khutsishvili I,Gold B,Marky LA

    更新日期:2018-10-02 00:00:00