Uracil DNA-glycosylase/glyceraldehyde-3-phosphate dehydrogenase is an Ap4A binding protein.

abstract：:The structures of beta class carbonic anhydrases (beta-CAs) determined so far fall into two distinct subclasses based on the observed coordination of the catalytic zinc (Zn2+) ion. The subclass of beta-CAs that coordinate Zn2+ tetrahedrally with four protein-derived ligands is represented by the structures of ortholog...

journal_title：Biochemistry

authors： Cronk JD,Rowlett RS,Zhang KY,Tu C,Endrizzi JA,Lee J,Gareiss PC,Preiss JR

更新日期：2006-04-11 00:00:00

abstract：:Residual dipolar couplings (RDCs) were used as restraints in fully solvated molecular dynamics simulations of reduced substrate- and carbonmonoxy-bound cytochrome P450(cam) (CYP101A1), a 414-residue soluble monomeric heme-containing camphor monooxygenase from the soil bacterium Pseudomonas putida. The (1)D(NH) residua...

journal_title：Biochemistry

authors： Asciutto EK,Dang M,Pochapsky SS,Madura JD,Pochapsky TC

更新日期：2011-03-15 00:00:00

abstract：:We have isolated from rabbit liver three cDNA clones of 1400-1800 base pairs that hybridize selectively to RNA from animals treated with phenobarbital. The nucleotide sequences of the cDNAs have been determined. In the protein coding region the nucleotide sequences of two of the cDNAs are 88% homologous, and the third...

journal_title：Biochemistry

authors： Leighton JK,DeBrunner-Vossbrinck BA,Kemper B

更新日期：1984-01-17 00:00:00

abstract：:Kinetic studies of reactions between fluorescein and antifluorescein antibody produced during early, intermediate, and late stages of the immune response have been carried out utilizing both fluorescence intensity and polarization measurements in the static (time constant similar to 5 sec) and in the stopped-flow mode...

journal_title：Biochemistry

authors： Levison SA,Hicks AN,Portmann AJ,Dandliker WB

更新日期：1975-08-26 00:00:00

abstract：:Oxindolealanine-62 lysozyme, formed by reaction with N-bromosuccinimide and purified by using affinity chromatography, was examined in its binding of homologous oligosaccharides of N-acetylglucosamine and in its catalysis of hydrolysis and of transglycosylation of the hexasaccharide of N-acetylglucosamine. Equilibrium...

journal_title：Biochemistry

authors： Shrake A,Rupley JA

更新日期：1980-08-19 00:00:00

abstract：:The idea of establishing the amyloid-like fibrillation tendency of pro- and antisurvival proteins of human apoptotic pathways is relevant for delineating the conditions that lead to aberrant differentiation, development, and tissue homeostasis. As the first step in this direction, we report here that the caspase recru...

journal_title：Biochemistry

authors： Rao PN,Reddy KS,Bhuyan AK

更新日期：2009-08-18 00:00:00

abstract：:Human alpha-lactalbumin (alpha-LA), a 123-residue calcium-binding protein, has been studied using (15)N NMR relaxation methods in order to characterize backbone dynamics of the native state at the level of individual residues. Relaxation data were collected at three magnetic field strengths and analyzed using the mode...

journal_title：Biochemistry

authors： Bruylants G,Redfield C

更新日期：2009-05-19 00:00:00

abstract：:Isolated chloroplasts are capable of synthesizing chloroplast DNA in the presence of Mg2+ and deoxynucleoside triphosphates. The in vitro reaction proceeds for at least 60 min and is inhibited by KC1 and N-ethylmaleimide. Stretches of several hundred nucleotides in length are synthesized within an hour. Little or no i...

journal_title：Biochemistry

authors： Zimmermann W,Weissbach A

更新日期：1982-07-06 00:00:00

abstract：:Hemopexin is a plasma glycoprotein that has two structural domains (I and II) and binds and transports heme particularly to liver cells. Differential scanning calorimetry (DSC) studies show that hemopexin is largely stabilized by heme, which binds exclusively to domain I. The melting temperature (Tm) of heme-hemopexin...

journal_title：Biochemistry

authors： Wu ML,Morgan WT

更新日期：1993-07-20 00:00:00

abstract：:There are 10 transmembrane protein (TMEM) 16-family proteins in humans and mice. Among them, TMEM16F acts as a Ca(2+)-dependent phospholipid scramblase at the plasma membrane. However, how Ca(2+) activates TMEM16F's phospholipid-scramblase activity has not been elucidated. Here we found that in the presence of Ca(2+),...

journal_title：Biochemistry

authors： Ishihara K,Suzuki J,Nagata S

更新日期：2016-06-14 00:00:00

abstract：:A heterologous expression system was used to evaluate activation of BlaR1, a sensor/signal transducer protein of Staphylococcus aureus with a central role in resistance to β-lactam antibiotics. In the absence of other S. aureus proteins that might respond to antibiotics and participate in signal transduction events, w...

journal_title：Biochemistry

authors： Llarrull LI,Mobashery S

更新日期：2012-06-12 00:00:00

abstract：:Interleukin-8 (IL-8), a member of the chemokine superfamily, exists as both monomers and dimers, and mediates its function by binding to neutrophil CXCR1 and CXCR2 receptors that belong to the G protein-coupled receptor class. It is now well established that the monomer functions as a high-affinity ligand, but the bin...

journal_title：Biochemistry

authors： Rajarathnam K,Prado GN,Fernando H,Clark-Lewis I,Navarro J

更新日期：2006-06-27 00:00:00

abstract：:AMSH, a deubiquitinating enzyme (DUB) with exquisite specificity for Lys63-linked polyubiquitin chains, is an endosome-associated DUB that regulates sorting of activated cell-surface signaling receptors to the lysosome, a process mediated by the members of the endosomal sorting complexes required for transport (ESCRT)...

journal_title：Biochemistry

authors： Davies CW,Paul LN,Das C

更新日期：2013-11-05 00:00:00

abstract：:We developed a general strategy for labeling expressed membrane proteins with a peptide epitope tag and detecting the tagged proteins in native cellular membranes. First, we genetically encoded the unnatural amino acid p-azido-L-phenylalanine (azF) at various specific sites in a G protein-coupled receptor (GPCR), C-C ...

journal_title：Biochemistry

authors： Naganathan S,Ye S,Sakmar TP,Huber T

更新日期：2013-02-12 00:00:00

abstract：:Phospholipids and sterols play multiple roles in cells. In addition to establishing barriers between compartments, they also provide the matrix for assembly and function of a large variety of catalytic processes. Lipid composition is a highly regulated feature of biological membranes, yet its implications for membrane...

journal_title：Biochemistry

authors： Autzen HE,Koldsø H,Stansfeld PJ,Gourdon P,Sansom MSP,Nissen P

更新日期：2018-07-17 00:00:00

abstract：:UDP-GlcNAc 2-epimerase/ManNAc kinase (GNE) catalyzes the first two committed steps in sialic acid synthesis. In addition to the three previously described human GNE isoforms (hGNE1-hGNE3), our database and polymerase chain reaction analysis yielded five additional human isoforms (hGNE4-hGNE8). hGNE1 is the ubiquitousl...

journal_title：Biochemistry

authors： Yardeni T,Choekyi T,Jacobs K,Ciccone C,Patzel K,Anikster Y,Gahl WA,Kurochkina N,Huizing M

更新日期：2011-10-18 00:00:00

abstract：:Flavo-diiron proteins (FDPs) function as anaerobic nitric oxide scavengers in some microorganisms, catalyzing reduction of nitric to nitrous oxide. The FDP from Thermotoga maritima can be prepared in a deflavinated form with an intact diferric site (deflavo-FDP). Hayashi et al. [(2010) Biochemistry 49, 7040-7049] repo...

journal_title：Biochemistry

authors： Caranto JD,Weitz A,Giri N,Hendrich MP,Kurtz DM Jr

更新日期：2014-09-09 00:00:00

abstract：:The iron K-edge X-ray absorption spectrum of Rhodococcus sp. R312 (formerly Brevibacterium sp. R312) nitrile hydratase in frozen solutions at pH 7 and 9 has been analyzed to determine details of the iron coordination. EXAFS analysis implies two or three sulfur ligands per iron and overall six coordination; together wi...

journal_title：Biochemistry

authors： Scarrow RC,Brennan BA,Cummings JG,Jin H,Duong DJ,Kindt JT,Nelson MJ

更新日期：1996-08-06 00:00:00

abstract：:Equine chorionic gonadotropin (eCG) and lutropin (eLH) are composed of alpha- and beta-subunits with an identical amino acid sequence but show different biological activities. To elucidate the molecular difference between these gonadotropins, the structure of the N-linked oligosaccharides of each beta-subunit was dete...

journal_title：Biochemistry

authors： Matsui T,Mizuochi T,Titani K,Okinaga T,Hoshi M,Bousfield GR,Sugino H,Ward DN

更新日期：1994-11-29 00:00:00

abstract：:The relaxin-like factor (RLF) is a circulating hormone that is synthesized in the gonads of mammals and released into the bloodstream. The distribution of its receptor and a trace of cross-reactivity to relaxin receptors implied that this relatively new factor is more relaxin- than insulin-like. The chemical synthesis...

journal_title：Biochemistry

authors： Büllesbach EE,Schwabe C

更新日期：1999-03-09 00:00:00

abstract：:We studied the binding of peptides containing five basic residues to membranes containing acidic lipids. The peptides have five arginine or lysine residues and zero, one, or two alanines between the basic groups. The vesicles were formed from mixtures of a zwitterionic lipid, phosphatidylcholine, and an acidic lipid, ...

journal_title：Biochemistry

authors： Mosior M,McLaughlin S

更新日期：1992-02-18 00:00:00

abstract：:Aminoimidazole riobnucleotide carboxylase, the sixth step in the purine biosynthetic pathway, catalyzes the conversion of aminoimidazole ribonucleotide (AIR) to carboxyaminoimidazole ribonucleotide (CAIR). The gene products of the purE and purK genes (PurE and PurK, respectively) thought to be responsible for this act...

journal_title：Biochemistry

authors： Meyer E,Leonard NJ,Bhat B,Stubbe J,Smith JM

更新日期：1992-06-02 00:00:00

abstract：:The translation of a small number of mRNAs in mouse SC-1 fibroblasts can be stimulated by cycloheximide, under conditions where the synthesis of most proteins is inhibited. These mRNAs are ordinarily present in small polyribosomes or messenger ribonucleoprotein particles, although the addition of cycloheximide drives ...

journal_title：Biochemistry

authors： Walden WE,Thach RE

更新日期：1986-04-22 00:00:00

abstract：:Initiation of RNA synthesis by Bacillus subtilis RNA polymerase (sigma-43) has been examined at two early promoters of phage phi 29: the A2 promoter, which is a weak promoter, and the G2 promoter, which is a strong promoter. The delta subunit of the polymerase inhibits the rate of initiation at A2, but not G2. In addi...

journal_title：Biochemistry

authors： Dobinson KF,Spiegelman GB

更新日期：1987-12-15 00:00:00

abstract：:We have used DNase I footprinting to examine the binding of five different 17-mer oligonucleotides to a 53-base oligopurine tract containing four pyrimidine interruptions. Although all the expected triplexes formed with high affinity (K(d) approximately 10-50 nM), one oligonucleotide produced a footprint at a second s...

journal_title：Biochemistry

authors： Fox KR,Flashman E,Gowers D

更新日期：2000-06-06 00:00:00

abstract：:The physical origin of the extremely high thermal stability of tetraether liposomes composed of the polar lipid fraction E (PLFE) from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius has been investigated. The leakage rate of trapped 5, 6-carboxyfluorescein (5(6)CF) and the proton permeability in PLFE ...

journal_title：Biochemistry

authors： Komatsu H,Chong PL

更新日期：1998-01-06 00:00:00

abstract：:Membrane fusion is involved in many fundamental cellular processes and entry of enveloped viruses into host cells. Influenza type A virus HA has long served as a paradigm for mechanistic studies of protein-mediated membrane fusion via large-scale structural rearrangements induced by acidic pH. Here we report the newly...

journal_title：Biochemistry

authors： Ni F,Chen X,Shen J,Wang Q

更新日期：2014-02-11 00:00:00

abstract：:Mouse photoreceptor function and survival critically depend on Ca(2+)-regulated retinal membrane guanylyl cyclase (RetGC), comprised of two isozymes, RetGC1 and RetGC2. We characterized the content, catalytic constants, and regulation of native RetGC1 and RetGC2 isozymes using mice lacking guanylyl cyclase activating ...

journal_title：Biochemistry

authors： Peshenko IV,Olshevskaya EV,Savchenko AB,Karan S,Palczewski K,Baehr W,Dizhoor AM

更新日期：2011-06-28 00:00:00

abstract：:The pentraxin serum amyloid P component (SAP) is secreted by the liver and found in plasma at a concentration of approximately 30 mg/L. SAP is a 25 kDa homopentamer known to bind both protein and nonprotein ligands, all in a calcium-dependent manner. The function of SAP is unclear but likely involves the humoral innat...

journal_title：Biochemistry

authors： Poulsen ET,Pedersen KW,Marzeda AM,Enghild JJ

更新日期：2017-02-14 00:00:00

abstract：:M proteins and other members of the M protein family, expressed on the surface of Streptococcus pyogenes, bind host proteins such as immunoglobulins, albumin, and fibrinogen. Protein H and the M1 protein are expressed by adjacent genes and both belong to the M protein family. In this work, the structure and stability ...

journal_title：Biochemistry

authors： Nilson BH,Frick IM,Akesson P,Forsén S,Björck L,Akerström B,Wikström M

更新日期：1995-10-17 00:00:00