Covalent structure of collagen: amino acid sequence of alpha 1(III)-CB9 from type III collagen of human liver.

abstract：:Purine C-8 tritium-labeling rates have been measured at specific sites in Escherichia coli tRNAIle and tRNA2Tyr. The results are compared with those obtained for yeast tRNAPhe (preceding paper(Gamble et al., 1976)). The tRNAIle and tRNAPhe fall into the same general class of tRNA structures, while tRNA2Tyr is in a dif...

journal_title：Biochemistry

authors： Schoemaker HJ,Gamble RC

更新日期：1976-06-29 00:00:00

abstract：:The roles of four of the active center groups with potential acid-base properties in the region of pH optimum of pyruvate decarboxylase from Saccharomyces cerevisiae have been studied with the substitutions Asp28Ala, His114Phe, His115Phe, and Glu477Gln, introduced by site-directed mutagenesis methods. The steady-state...

journal_title：Biochemistry

authors： Liu M,Sergienko EA,Guo F,Wang J,Tittmann K,Hübner G,Furey W,Jordan F

更新日期：2001-06-26 00:00:00

abstract：:We have used nucleoside phosphorothioates (NTP alpha S) and a substitution-interference method to identify phosphate oxygens that appear to be important to guanosine cofactor addition in the self-splicing group I intron from Tetrahymena thermophila. For the majority of these phosphate oxygens, however, the effect of N...

journal_title：Biochemistry

authors： Christian EL,Yarus M

更新日期：1993-05-04 00:00:00

abstract：:A dipeptide containing the binding site for retinal in bovine rhodopsin has been isolated and its sequence determined. Rhodopsin containing [11-3H]retinal was prepared in chromatographically pure form, and the [3H]retinal was reductively linked to its binding site on opsin by using borane--dimethylamine. The [3H]retin...

journal_title：Biochemistry

authors： Wang JK,McDowell JH,Hargrave PA

更新日期：1980-10-28 00:00:00

abstract：:Apolipoprotein E (apoE)-knockout mice develop severe atherosclerosis associated with high levels of very low-density lipoprotein (VLDL) and intermediate-density lipoprotein (IDL) in plasma. To investigate the atherogenic role of VLDL and IDL, the lipoprotein fraction containing both VLDL and IDL (apoEko-VLDL/IDL) was ...

journal_title：Biochemistry

authors： Hakamata H,Sakaguchi H,Zhang C,Sakashita N,Suzuki H,Miyazaki A,Takeya M,Takahashi K,Kitamura N,Horiuchi S

更新日期：1998-09-29 00:00:00

abstract：:RalA and RalB are members of the Ras family of small G proteins and are activated downstream of Ras via RalGEFs. The RalGEF-Ral axis represents one of the major effector pathways controlled by Ras and as such is an important pharmacological target. RalA and RalB are approximately 80% identical at the amino acid level;...

journal_title：Biochemistry

authors： Campbell LJ,Peppa M,Crabtree MD,Shafiq A,McGough NF,Mott HR,Owen D

更新日期：2015-02-17 00:00:00

abstract：:Ethylene and its analogues acetylene, carbon monoxide, and propylene inhibited the rate of oxidation of indole-3-acetic acid by peroxidase. Annulment of this effect by addition of superoxide dismutase showed that inhibition occurred only in the presence of the superoxide anion radical (O2-.). Kinetic and spectral data...

journal_title：Biochemistry

authors： Smith AM,Morrison WL,Milham PJ

更新日期：1983-03-29 00:00:00

abstract：:The solution secondary structure of the highly active Y55F/Y88F "Tyr-14-only" mutant of delta 5-3-ketosteroid isomerase complexed with 19-nortestosterone hemisuccinate has been shown to consist of three helices, a six-stranded mixed beta-sheet, and five turns. The steroid binds near the general acid, Tyr-14, on helix ...

journal_title：Biochemistry

authors： Zhao Q,Abeygunawardana C,Gittis AG,Mildvan AS

更新日期：1997-12-02 00:00:00

abstract：:Papain-solubilized human class II (HLA-DR) antigens have been purified from cadaveric spleens by ion-exchange chromatography, gel chromatography, and immunosorbent purification. The isolated papain-solubilized antigens comprised two subunits with apparent molecular weights of 23 000 and 30 000, respectively. The circu...

journal_title：Biochemistry

authors： Wiman K,Claesson L,Rask L,Trägårdh L,Peterson PA

更新日期：1982-10-12 00:00:00

abstract：:The specific deamidation of asparagine-71 of triosephosphate isomerase increases upon substrate binding and catalysis. This deamidation at the dimer interface initiates subunit dissociation, unfolding, and protein degradation. The apparent connection between catalysis and terminal marking supports the concept of "mole...

journal_title：Biochemistry

authors： Garza-Ramos G,Tuena de Gomez-Puyou M,Gomez-Puyou A,Yüksel KU,Gracy RW

更新日期：1994-06-07 00:00:00

abstract：:Nitridomanganese(V) protoporphyrin IX was prepared by hypochlorite oxidation of the corresponding manganese(III) protoporphyrin IX derivative in the presence of ammonium ion and by photolysis of the corresponding azidomanganese(III) complex. Myoglobin and horseradish peroxidase containing this novel protoporphyrin der...

journal_title：Biochemistry

authors： Tsubaki M,Hori H,Hotta T,Hiwatashi A,Ichikawa Y,Yu NT

更新日期：1987-08-11 00:00:00

abstract：:3-Fluorooxalacetate is a substrate for malic dehydrogenase. When enzymatic reduction is slower than the rate of epimerization of the two enantiomers, only (2R,3R)-erythro-fluoromalate is formed. Conversely, when a high enzyme level and excess of NADH lead to reduction that is fast relative to the epimerization rate, e...

journal_title：Biochemistry

authors： Urbauer JL,Bradshaw DE,Cleland WW

更新日期：1998-12-22 00:00:00

abstract：:Guanidinium chloride- (GdmCl-) induced unfolding of beta-lactamase has been investigated by a combination of size-exclusion chromatography (SEC-FPLC) and usual optical methods. It has been shown that at low temperatures this protein unfolds through two equilibrium intermediates. The first of these intermediates is the...

journal_title：Biochemistry

authors： Uversky VN,Ptitsyn OB

更新日期：1994-03-15 00:00:00

abstract：:Recombinant human 3'-phosphoadenosine 5'-phosphosulfate (PAPS) synthetase, isoform 1 (brain), was purified to near-homogeneity from an Escherichia coli expression system and kinetically characterized. The native enzyme, a dimer with each 71 kDa subunit containing an adenosine triphosphate (ATP) sulfurylase and an aden...

journal_title：Biochemistry

authors： Lansdon EB,Fisher AJ,Segel IH

更新日期：2004-04-13 00:00:00

abstract：:The individual amide proton exchange rates in Tendamistat at pH 3.0 and 50 degrees C were measured by using two-dimensional 1H nuclear magnetic resonance. Overall, it was found that the distribution of exchange rates along the sequence is dominated by the interstrand hydrogen bonds of the beta-sheet structures. The sl...

journal_title：Biochemistry

authors： Wang QW,Kline AD,Wüthrich K

更新日期：1987-10-06 00:00:00

abstract：:Protein folding is a process in which an extended polypeptide chain acquires compact packing through the formation of specific secondary and tertiary structures and hydrophobic interactions. Although much attention has been paid to secondary and tertiary structures, there is no definitive view about the relationship b...

journal_title：Biochemistry

authors： Nishii I,Kataoka M,Tokunaga F,Goto Y

更新日期：1994-04-26 00:00:00

abstract：:Drug intervention that prevents reabsorption of circulating bile acids by the apical (ileal) sodium/bile acid cotransporter (ASBT) may be a promising new therapy for lowering of plasma cholesterol. 2164U90 is a benzothiazepine-based competitive inhibitor of bile acid transport with K(i) values of approximately 10 and ...

journal_title：Biochemistry

authors： Hallén S,Björquist A,Ostlund-Lindqvist AM,Sachs G

更新日期：2002-12-17 00:00:00

abstract：:C-type natriuretic peptide (CNP) binds the guanylyl cyclase-linked natriuretic peptide receptor B (NPR-B) and stimulates marked elevations of the intracellular signaling molecule, cGMP. Here, the essential role of phosphorylation in the hormonal activation and deactivation of this receptor is described. Exposure of NI...

journal_title：Biochemistry

authors： Potter LR

更新日期：1998-02-24 00:00:00

abstract：:A molecular model has been built of the equine infectious anemia virus (EIAV) proteinase on the basis of the crystal structures of the related Rous sarcoma virus (RSV) and human immunodeficiency virus (HIV) proteinases. The 104 residue long EIAV proteinase has 30 identical and 11 similar amino acids compared to those ...

journal_title：Biochemistry

authors： Weber IT,Tözsér J,Wu J,Friedman D,Oroszlan S

更新日期：1993-04-06 00:00:00

abstract：:Indolethylamine-N-methyltransferase (INMT) is a Class 1 transmethylation enzyme known for its production of N,N-dimethyltryptamine (DMT), a hallucinogen with affinity for various serotonergic, adrenergic, histaminergic, dopaminergic, and sigma-1 receptors. DMT is produced via the action of INMT on the endogenous subst...

journal_title：Biochemistry

authors： Chu UB,Vorperian SK,Satyshur K,Eickstaedt K,Cozzi NV,Mavlyutov T,Hajipour AR,Ruoho AE

更新日期：2014-05-13 00:00:00

abstract：:Nonenzymatic deamidation of asparagine and glutamine in peptides and proteins is a frequent modification both in vivo and in vitro. The biological effect is not completely understood, but it is often associated with protein degradation and loss of biological function. Here we describe the deamidation of CsgA, the majo...

journal_title：Biochemistry

authors： Wang H,Shu Q,Frieden C,Gross ML

更新日期：2017-06-13 00:00:00

abstract：:Mammalian type B (mitochondrial) b(5) cytochromes exhibit greater amino acid sequence diversity than their type A (microsomal) counterparts, as exemplified by the type B proteins from human (hCYB5B) and rat (rCYB5B). The comparison of X-ray crystal structures of hCYB5B and rCYB5B reported herein reveals a striking dif...

journal_title：Biochemistry

authors： Parthasarathy S,Altuve A,Terzyan S,Zhang X,Kuczera K,Rivera M,Benson DR

更新日期：2011-06-21 00:00:00

abstract：:Kynureninase is a pyridoxal 5'-phosphate-dependent enzyme that catalyzes the hydrolytic cleavage of l-kynurenine to give l-alanine and anthranilic acid. beta-Benzoyl-l-alanine, the analogue of l-kynurenine lacking the aromatic amino group, was shown to a good substrate for kynureninase from Pseudomonas fluorescens, an...

journal_title：Biochemistry

authors： Kumar S,Gawandi VB,Capito N,Phillips RS

更新日期：2010-09-14 00:00:00

abstract：:The effect of anions on the stability of different functional conformations of Hb is examined through the determination of the dependence of O(2) affinity on water activity (a(w)). The control of a(w) is effected by varying the sucrose osmolal concentration in the bathing solution according to the "osmotic stress" met...

journal_title：Biochemistry

authors： Colombo MF,Seixas FA

更新日期：1999-09-07 00:00:00

abstract：:The phosphatase activity of the homodimeric NRII protein of Escherichia coli is activated by the PII protein and requires all three domains of NRII. Mutations in the N-terminal domain (L16R), central domain (A129T), C-terminal domain PII-binding site (S227R), and C-terminal domain ATP-lid (Y302N) of NRII result in dim...

journal_title：Biochemistry

authors： Pioszak AA,Ninfa AJ

更新日期：2003-07-29 00:00:00

abstract：:Calcium and its receptor protein calmodulin function in the regulation of proliferation of mammalian cells. A 68 kDa calmodulin-specific binding protein was shown previously to be associated with growth factor-dependent progression of a variety of mammalian cells from G1 to S phase and to stimulate DNA synthesis in pe...

journal_title：Biochemistry

authors： Cao QP,McGrath CA,Baril EF,Quesenberry PJ,Reddy GP

更新日期：1995-03-28 00:00:00

abstract：:The Escherichia coli L-arabinose-binding protein (ABP) participates as a specific receptor in the transport of L-arabinose, D-fucose, and D-galactose through the periplasmic space. The wild-type protein binds L-arabinose about 40 times more strongly than D-fucose. A mutation of the protein at position 108 (Met-->Leu) ...

journal_title：Biochemistry

authors： Zacharias M,Straatsma TP,McCammon JA,Quiocho FA

更新日期：1993-07-27 00:00:00

abstract：:SHP-1, a protein tyrosine phosphatase containing two tandem SH2 domains, is autoinhibited at rest by its N-terminal SH2 (N-SH2) domain. Relief from autoinhibition and a subsequent 10-60-fold increase in V(max) have been observed upon N-SH2 domain engagement by a specific phosphotyrosyl ligand or upon deletion of the S...

journal_title：Biochemistry

authors： Wang J,Walsh CT

更新日期：1997-03-11 00:00:00

abstract：:We have determined the mechanism of DNA-dependent protein kinase (DNA-PK) inhibition by cis-diamminedichloroplatinum(II)-(cisplatin-) damaged DNA. We previously have demonstrated that Ku, the DNA binding subunit of DNA-PK, is capable of binding to DNA duplexes globally damaged with cisplatin but was unable to stimulat...

journal_title：Biochemistry

authors： Turchi JJ,Patrick SM,Henkels KM

更新日期：1997-06-17 00:00:00

abstract：:Lamin A protein, encoded by the LMNA gene, belongs to the type V intermediate filament protein family and is a major nuclear protein component of higher metazoan organisms, including humans. Lamin A along with B-type lamins impart structural rigidity to the nucleus by forming a lamina that is closely apposed to the in...

journal_title：Biochemistry

authors： Bhattacharjee P,Banerjee A,Banerjee A,Dasgupta D,Sengupta K

更新日期：2013-06-18 00:00:00