Nuclear magnetic resonance studies on transfer ribonucleic acid: assignment of AU tertiary resonances.

abstract：:The role of the "C-terminal tail" segment of long neurotoxins has been investigated. The C-terminal four to five residues of alpha-bungarotoxin and Laticauda colubrina b have been cleaved off by carboxypeptidase P. The effect of such deletion on the toxin conformation has been monitored in proton nuclear magnetic reso...

journal_title：Biochemistry

authors： Endo T,Oya M,Tamiya N,Hayashi K

更新日期：1987-07-14 00:00:00

abstract：:The sialic acid binding lectin, limulin, was isolated by gel filtration and ion-exchange chromatography from the hemolymph of Limulus polyphemus. When the purified protein was characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis in the presence of beta-mercaptoethanol, two major protein bands wer...

journal_title：Biochemistry

authors： Kaplan R,Li SS,Kehoe JM

更新日期：1977-09-20 00:00:00

abstract：:Tau is a microtubule associated protein implicated in the pathogenesis of several neurodegenerative diseases. Because of the channel forming properties of other amyloid peptides, we employed planar lipid bilayers and atomic force microscopy to test tau for its ability to form ion permeable channels. Our results demons...

journal_title：Biochemistry

authors： Patel N,Ramachandran S,Azimov R,Kagan BL,Lal R

更新日期：2015-12-22 00:00:00

abstract：:Cyanobactin heterocyclases share the same catalytic domain (YcaO) as heterocyclases/cyclodehydratases from other ribosomal peptide (RiPPs) biosynthetic pathways. These enzymes process multiple residues (Cys/Thr/Ser) within the same substrate. The processing of cysteine residues proceeds with a known order. We show the...

journal_title：Biochemistry

authors： Ge Y,Czekster CM,Miller OK,Botting CH,Schwarz-Linek U,Naismith JH

更新日期：2019-04-23 00:00:00

abstract：:Four murine myeloma immunoglobulins, A4, A47N, U61, and E109, have been studied for their binding affinities with inulin and a series of oligosaccharides derived from inulin. The results indicate that the combining site of these immunoglobulins shows highest complementarity for a trifructofuranosyl sequence (A4 and A4...

journal_title：Biochemistry

authors： Streefkerk DG,Glaudemans CP

更新日期：1977-08-23 00:00:00

abstract：:The tiny picoalga, Ostreococcus tauri, originating from the Thau Lagoon is a member of the marine phytoplankton. Because of its highly reduced genome and small cell size, while retaining the fundamental requirements of a eukaryotic photosynthetic cell, it became a popular model organism for studying photosynthesis or ...

journal_title：Biochemistry

authors： Luck M,Velázquez Escobar F,Glass K,Sabotke MI,Hagedorn R,Corellou F,Siebert F,Hildebrandt P,Hegemann P

更新日期：2019-04-09 00:00:00

abstract：:FixL is a sensor histidine kinase having a heme-containing domain as an O(2) sensing site. In the study presented here, Ile209 and Ile210 located near the heme iron of the heme domain of Rhizobium meliloti FixL (RmFixL) were mutated, and the mutational effects on the regulation of the kinase activity and the heme pock...

journal_title：Biochemistry

authors： Mukai M,Nakamura K,Nakamura H,Iizuka T,Shiro Y

更新日期：2000-11-14 00:00:00

abstract：:Cibacron Blue F3GA dye has been used to probe subtle conformational changes in protein structure associated with the conversion of Escherichia coli glutamine synthetase (GS) between relaxed, taut, oxidized, and dissociated forms. Binding of the dye to each form of the enzyme elicits a different spectral perturbation o...

journal_title：Biochemistry

authors： Federici MM,Chock PB,Stadtman ER

更新日期：1985-01-29 00:00:00

abstract：:It is known that the proapoptotic protein Bax facilitates the formation of pores in bilayers, resulting in the release of proteins from the intermitochondrial space. We demonstrate that another consequence of the interaction of Bax with membranes is an increase in the rate of lipid transbilayer diffusion. We use two i...

journal_title：Biochemistry

authors： Epand RF,Martinou JC,Montessuit S,Epand RM

更新日期：2003-12-16 00:00:00

abstract：:The peptide hormone bradykinin exerts important biological functions by binding to and activating bradykinin B2 receptors. B2 receptors belong to the seven transmembrane domain (7TM) receptor family. Cloning of the cDNA sequences for the rat, human, and mouse bradykinin B2 receptor revealed several in-frame AUG triple...

journal_title：Biochemistry

authors： AbdAlla S,Godovac-Zimmermann J,Braun A,Roscher AA,Müller-Esterl W,Quitterer U

更新日期：1996-06-11 00:00:00

abstract：:An alamethicin, secreted by the fungus Trichoderma viride and containing a glutamine at position 18 instead of the usual glutamic acid, has been uniformly labeled with 15N and purified by HPLC. The extent of 15N incorporation at individual backbone and side-chain sites was found to vary from 85% to 92%, as measured by...

journal_title：Biochemistry

authors： Yee AA,O'Neil JD

更新日期：1992-03-31 00:00:00

abstract：:6-Hydroxymethyl-7,8-dihydropterin pyrophosphokinase (HPPK) catalyzes the pyrophosphoryl transfer from ATP to 6-hydroxymethyl-7,8-dihydropterin (HP), the first reaction in the folate biosynthetic pathway. Arginine residues 82 and 92, strictly conserved in 35 HPPK sequences, play dynamic roles in the catalytic cycle of ...

journal_title：Biochemistry

authors： Blaszczyk J,Li Y,Shi G,Yan H,Ji X

更新日期：2003-02-18 00:00:00

abstract：:New isoforms of CYP51 (sterol 14alpha-demethylase), an essential enzyme in sterol biosynthesis and primary target of azole antimycotic drugs, are found in pathogenic protists, Trypanosoma brucei(TB), T. vivax, T. cruzi, and Leishmania major. The sequences share approximately 80% amino acid identity and are approximate...

journal_title：Biochemistry

authors： Lepesheva GI,Nes WD,Zhou W,Hill GC,Waterman MR

更新日期：2004-08-24 00:00:00

abstract：:Drosophila Rrp1 includes a carboxy-terminal region homologous to Escherichia coli exonuclease III which is sufficient to repair both oxidative and alkylation damage to DNA. An apurinic/apyrimidinic endonuclease activity intrinsic to Rrp1 was characterized previously. In this work, the 3'-phosphodiesterase and 3'-phosp...

journal_title：Biochemistry

authors： Sander M,Huang SM

更新日期：1995-01-31 00:00:00

abstract：:The catalysis of the chemical exchange between acetaldehyde and its hydrate by bovine carbonic anhydrase B was investigated by the NMR line broadening and saturation transfer techniques. While both chemical exchange and binding to the enzyme had an effect on the line broadening of acetaldehyde and its hydrate, the sat...

journal_title：Biochemistry

authors： Cheshnovsky D,Navon G

更新日期：1980-04-29 00:00:00

abstract：:Three distinct fibrinolytic enzymes have been purified from the venom of Crotalus basiliscus basiliscus (the Mexican west coast rattlesnake). The high-performance liquid chromatography-based purification comprised the following steps: (a) hydrophobic interaction chromatography; (b) hydroxylapatite chromatography; (c) ...

journal_title：Biochemistry

authors： Retzios AD,Markland FS Jr

更新日期：1992-05-19 00:00:00

abstract：:The reaction between chymotrypsin and N-acetyl-L-phenylalanine p-nitroanilide has been studied at subzero temperatures in fluid aqueous dimethyl sulfoxide solvent. Following initiation of the reaction at temperatures as low as -90 degrees C, a series of four reactions prior to the normal rate-limiting step (acylation)...

journal_title：Biochemistry

authors： Fink AL

更新日期：1976-04-06 00:00:00

abstract：:Ceruloplasmin is unique among the multicopper oxidases in that in addition to the usual copper stoichiometry of one Type 1 copper site and a Type 2/Type 3 trinuclear copper cluster, it contains two other Type 1 sites. This assignment of copper sites, based on copper quantitation, sequence alignment, and crystallograph...

journal_title：Biochemistry

authors： Machonkin TE,Zhang HH,Hedman B,Hodgson KO,Solomon EI

更新日期：1998-06-30 00:00:00

abstract：:Calpain-catalyzed proteolysis of II-spectrin is a regulated event associated with neuronal long-term potentiation, platelet and leukocyte activation, and other processes. Calpain proteolysis is also linked to apoptotic and nonapoptotic cell death following excessive glutamate exposure, hypoxia, HIV-gp120/160 exposure,...

journal_title：Biochemistry

authors： Glantz SB,Cianci CD,Iyer R,Pradhan D,Wang KK,Morrow JS

更新日期：2007-01-16 00:00:00

abstract：:The cyclic antimicrobial lipopeptide daptomycin is now frequently used as a first-line therapy in serious infections caused by multidrug-resistant Enterococcus faecium. Resistance to daptomycin in E. faecium is mediated by activation of the LiaFSR membrane stress response pathway. Deletion of liaR, encoding the respon...

journal_title：Biochemistry

authors： Davlieva M,Wu C,Zhou Y,Arias CA,Shamoo Y

更新日期：2018-12-11 00:00:00

abstract：:Although host protein synthesis is preferentially inhibited, there is a steady decline in the ability of Chinese hamster ovary (CHO) cells infected with vesicular stomatitis virus (VSV) to synthesize both host and viral proteins. We previously reported finding an mRNA-ribonucleoprotein particle (mRNP) that contained a...

journal_title：Biochemistry

authors： Rosen CA,Siekierka J,Ennis HL,Cohen PS

更新日期：1984-05-22 00:00:00

abstract：:The sialic acid N-acetylneuraminic acid (NANA) has a key role in the pathogenesis of a select number of neuroinvasive bacteria such as Neisseria meningitidis. These pathogens coat themselves with polysialic acids, mimicking the exterior surface of mammalian cells and consequentially concealing the bacteria from the ho...

journal_title：Biochemistry

authors： Joseph DD,Jiao W,Parker EJ

更新日期：2013-04-16 00:00:00

abstract：:Oxidation of the biological antioxidant alpha-tocopherol (vitamin E; TH) by peroxyl radicals yields 8a-(alkyldioxy)tocopherones, which either may hydrolyze to alpha-tocopheryl quinone (TQ) or may be reduced by ascorbic acid to regenerate TH. To define the chemistry of this putative two-electron TH redox cycle, we stud...

journal_title：Biochemistry

authors： Liebler DC,Kaysen KL,Kennedy TA

更新日期：1989-12-12 00:00:00

abstract：:A strategy to detect P-glycoprotein (P-gp) on cell membrane and quantify the cell number using electrochemical immunoassay was developed by effective surface immunoreactions and immobilization of cells on a highly hydrophilic interface, which was constructed by adsorption of colloidal gold nanoparticles on a methoxysi...

journal_title：Biochemistry

authors： Du D,Ju H,Zhang X,Chen J,Cai J,Chen H

更新日期：2005-08-30 00:00:00

abstract：:CD4 is expressed on the surface of specific leukocytes where it plays a key role in the activation of immunostimulatory T-cells and acts as a primary receptor for HIV-1 entry. CD4 has four ecto-domains (D1-D4) of which D1, D2, and D4 contain disulfide bonds. Although disulfide bonds commonly serve structural or cataly...

journal_title：Biochemistry

authors： Owen GR,Channell JA,Forsyth VT,Haertlein M,Mitchell EP,Capovilla A,Papathanasopoulos M,Cerutti NM

更新日期：2016-04-19 00:00:00

abstract：:Hemoglobins extracted from fishes that live in temperate waters show little or no dissociation even in the liganded form, unlike human hemoglobin (HbA). To establish whether cold adaptation influences the tendency to dissociate, the dimer-tetramer association constants (L(2,4)) of the carbonmonoxy derivatives of repre...

journal_title：Biochemistry

authors： Giangiacomo L,D'Avino R,di Prisco G,Chiancone E

更新日期：2001-03-13 00:00:00

abstract：:The structure of an active mutant of (S)-mandelate dehydrogenase (MDH-GOX2) from Pseudomonas putida has been determined at 2.15 A resolution. The membrane-associated flavoenzyme (S)-mandelate dehydrogenase (MDH) catalyzes the oxidation of (S)-mandelate to give a flavin hydroquinone intermediate which is subsequently r...

journal_title：Biochemistry

authors： Sukumar N,Xu Y,Gatti DL,Mitra B,Mathews FS

更新日期：2001-08-21 00:00:00

abstract：:We determined the magnitude of the bend induced in DNA by an adenine-thymine tract by measuring the rate of cyclization of DNA oligonucleotides containing phased A tracts. A series of linear multimers with 2-bp single-stranded ends, in which the (A.T)6 tracts are separated by CG2-3C sequences and are positioned 10 and...

journal_title：Biochemistry

authors： Koo HS,Drak J,Rice JA,Crothers DM

更新日期：1990-05-01 00:00:00

abstract：:Six affinity reagents containing chemically reactive groups, either on the phosphate residue at the 5'-end or on the 5'- or 3'-end internucleoside phosphate linkages of the oligothymidylate primers, were used to covalently modify the human immunodeficiency virus type 1 reverse transcriptase (HIV-1 RT). After covalent ...

journal_title：Biochemistry

authors： Andreola ML,Tarrago-Litvak L,Levina AS,Kolocheva TI,el Dirani-Diab R,Jamkovoy VI,Khalimskaya NL,Barr PJ,Litvak S,Nevinsky GA

更新日期：1993-04-13 00:00:00

abstract：:The photochemical intermediate metarhodopsin II (meta II; lambda max = 380 nm) is generally identified with rho*, the conformation of photolyzed rhodopsin which binds and activates the visual G-protein, Gt [Emeis, D., & Hoffman, K.P. (1981) FEBS Lett. 136, 201-207]. Purified bovine rhodopsin was incorporated into vesi...

journal_title：Biochemistry

authors： Mitchell DC,Kibelbek J,Litman BJ

更新日期：1991-01-08 00:00:00