Abstract：
:Choline acetyltransferase (EC 2.3.1.6) catalyzes the biosynthesis of acetylcholine according to the following chemical equation: acetyl-CoA + choline in equilibrium to acetylcholine + CoA. In addition to nervous tissue, primate placenta is the only other animal source which contains appreciable acetylcholine and its biosynthetic enzyme. Human brain caudate nucleus and human placental choline acetyltransferase were purified to electrophoretic homogeneity using ion-exchange and blue dextran-Sepharose affinity chromatography. The molecular weights determined by Sephadex G-150 gel filtration and sodium dodecyl sulfate gel electrophoresis are 67000 plus or minus 3000. N-Ethylmaleimide, p-chloromercuribenzoate, and dithiobis(2-nitrobenzoic acid) inhibit the enzyme. Dithiothreitol reverses the inhibition produced by the latter two reagents. The pKa of the group associated with N-ethylmaleimide inhibition is 8.6 plus or minus 0.3. A chemically competent acetyl-thioenzyme is isolable by Sephadex gel filtration. The enzymes from the brain and placenta are thus far physically and biochemically indistinguishable.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Roskoski R Jr,Lim CT,Roskoski LMdoi
10.1021/bi00694a013subject
Has Abstractpub_date
1975-11-18 00:00:00pages
5105-10issue
23eissn
0006-2960issn
1520-4995journal_volume
14pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:In this study, we prepared several shortened and full-length insulin analogues with substitutions at position B26. We compared the binding affinities of the analogues for rat adipose membranes with their ability to lower the plasma glucose level in nondiabetic Wistar rats in vivo after subcutaneous administration, and...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi702086w
更新日期：2008-05-27 00:00:00
abstract：:In this paper, we report kinetic studies for the chymotryptic activity of the 20S proteasome. Major observations include the following: (1) Reaction progress curves that are recorded at concentrations of Suc-Leu-Leu-Val-Tyr-AMC greater than about 40 microM are biphasic and characterized by initial velocities that deca...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi952262x
更新日期：1996-04-02 00:00:00
abstract：:The bovine prothrombin gene was characterized by Southern blot analysis of bovine genomic DNA using bovine prothrombin cDNA fragments as hybridization probes. These analyses suggested that the bovine genome contains a single prothrombin gene that is at least 10 kilobase pairs (kbp) in size. To characterize the gene mo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00345a018
更新日期：1985-11-19 00:00:00
abstract：:Earlier work indicates that the major DNA repair phosphodiesterase (PDE) in yeast cells is the well-characterized Apn1 protein. Apn1 demonstrates both Mg2+-independent PDE activity and Mg2+-independent class II apurinic/apyrimidinic (AP) endonuclease activity and represents greater than 90% of the activity detected in...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi970048y
更新日期：1997-05-20 00:00:00
abstract：:Tubulin polymerization promoting proteins (TPPPs) constitute a eukaryotic protein family. There are three TPPP paralogs in the human genome, denoted as TPPP1-TPPP3. TPPP1 and TPPP3 are intrinsically unstructured proteins (IUPs) that bind and polymerize tubulin and stabilize microtubules, but TPPP2 does not. Vertebrate...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00902
更新日期：2017-02-21 00:00:00
abstract：:The heme and cysteine microenvironments of bluefin tuna apomyoglobin have been investigated by examining the fluorescence properties of two extrinsic chromophores, i.e., ANS and 1,5-AEDANS. 1,5-AEDANS was covalently bound to the single cysteine residue found in the primary structure of tuna apomyoglobin. Recombination...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00531a002
更新日期：1982-01-19 00:00:00
abstract：:X-ray crystal structures of enzyme-ligand complexes are widely believed to mimic states in the catalytic cycle, but this presumption has seldom been carefully scrutinized. In the case of Tritrichomonas foetus inosine 5'-monophosphate dehydrogenase (IMPDH), 10 structures of various enzyme-substrate-inhibitor complexes ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi101590c
更新日期：2010-12-21 00:00:00
abstract：:Aromatic amino acid aminotransferase (ArAT) from Escherichia coli was overexpressed in E. coli cells, purified, and characterized. The enzyme was similar to aspartate aminotransferase (AspAT) of E. coli in many aspects, such as gross protein structure and spectroscopic properties. The reactions of pyridoxal 5'-phospha...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00096a036
更新日期：1993-11-16 00:00:00
abstract：:The composition of the molybdate-stabilized glucocorticoid receptor (GR) complex has been investigated with a monoclonal antibody against the steroid-binding Mr 94 000 (94K) GR protein. It was concluded that one antibody molecule binds one 94K GR molecule. This finding constituted the basis for calculating the number ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00344a041
更新日期：1985-11-05 00:00:00
abstract：:The intracellular kinetic folding pathway of the human chorionic gonadotropin beta-subunit (hCG-beta) reveals the presence of a disulfide between Cys residues 38-57 that is not detected by X-ray analysis of secreted hCG-beta. This led us to propose that disulfide rearrangement is an essential feature of cystine knot f...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi049856x
更新日期：2004-05-04 00:00:00
abstract：:The (beta alpha)8 (or "TIM")-barrel protein phosphoribosylanthranilate isomerase from Saccharomyces cerevisiae was cleaved between the sixth and seventh beta alpha module to test the capacity of the resulting fragments to adopt native format autonomously. The fragments, which were expressed from separate coding sequen...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00129a010
更新日期：1992-04-14 00:00:00
abstract：:Kinetic studies of the effect of addition of Co2+ or Mn2+ to a highly purified dipeptidase from Ehrlich-Lettré mouse ascites tumor cells show that these metals specifically activate the hydrolyses of certain classes of dipeptides. This enzyme was previously (S. Hayman and E. K. Patterson, 1971, J. Biol. Chem. 246, 660...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00690a018
更新日期：1975-09-23 00:00:00
abstract：:The capsular polysaccharide (CPS) structure of Campylobacter jejuni contributes to its robust fitness. Many strains contain heptose moieties in their CPS units. The precursor heptose is GDP-d-glycero-α-d-manno-heptose; modifications to the stereochemistry at C3-C6 as well as additions of methyl and phosphoramidate gro...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.9b00548
更新日期：2019-09-17 00:00:00
abstract：:The proteins which have been left tightly bound to the tissue culture substrate after ethylenebis (oxyethyl-enenitrilo) tetraacetic acid (EGTA)-mediated removal of normal, virus-transformed, and revertant mouse cells and which have been implicated in the substrate adhesion process have been analyzed by slab sodium dod...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00663a028
更新日期：1976-09-07 00:00:00
abstract：:Lipoprotein lipase (LPL) is a dimeric enzyme that is responsible for clearing triglyceride-rich lipoproteins from the blood. Although LPL plays a key role in cardiovascular health, an experimentally derived three-dimensional structure has not been determined. Such a structure would aid in understanding mutations in LP...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.7b01009
更新日期：2018-01-16 00:00:00
abstract：:The envelope glycoprotein (G protein) of vesicular stomatitis virus probably exists in the viral envelope as a trimer of identical subunits. Depending on the conditions of solubilization, G protein may dissociate into monomers. G protein solubilized with the detergent octyl glucoside was shown to exist as oligomeric f...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00462a002
更新日期：1990-03-13 00:00:00
abstract：:The first positive evidence for the utilization of a direct C-6' ' oxidation/reduction mechanism by ADP-l-glycero-d-manno-heptose 6-epimerase is reported here. The epimerase (HldD or AGME, formerly RfaD) operates in the biosynthetic pathway of l-glycero-d-manno-heptose, which is a conserved sugar in the core region of...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi050106c
更新日期：2005-04-19 00:00:00
abstract：:Treatment of the beta 2 adrenergic receptor with the reducing agent dithiothreitol (DTT) is known to abolish ligand binding to the receptor. Interestingly, the loss of binding can be prevented by preoccupation of the receptor with ligand. It is unclear, however, whether the ligand blocks access of DTT to the receptor,...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961619+
更新日期：1996-11-19 00:00:00
abstract：:A method for the rapid and quantitative analysis of 5'-terminal oligonucleotides of RNAs made in vitro is described. The method involves synthesis of RNA in the presence of [gamma-32P]ATP or GTP, isolation of the RNA, and digestion with T1 or pancreatic ribonucleases to release labeled 5'-triphosphate termanated oligo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00604a005
更新日期：1978-05-30 00:00:00
abstract：:We measure the effects of low concentrations of helix-stabilizing cosolvents, including 2,2,2-trifluoroethanol (TFE), on the thermodynamics and kinetics of folding of the dimeric alpha-helical coiled coil derived from the leucine zipper region of bZIP transcriptional activator GCN4. The change in kinetic behavior upon...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi981641y
更新日期：1998-10-13 00:00:00
abstract：:Photolyases (PHRs) repair the UV-induced photoproducts, cyclobutane pyrimidine dimer (CPD) or pyrimidine-pyrimidone (6-4) photoproduct [(6-4) PP], restoring normal bases to maintain genetic integrity. CPD and (6-4) PP are repaired by substrate-specific PHRs, CPD PHR and (6-4) PHR, respectively. Flavin adenine dinucleo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b01111
更新日期：2016-02-02 00:00:00
abstract：:This study was undertaken to identify the alpha-helical domains of human apoE that mediate cellular cholesterol efflux and HDL assembly via ATP-binding cassette transporter A1 (ABCA1). The C-terminal (CT) domain (residues 222-299) of apoE was found to stimulate ABCA1-dependent cholesterol efflux in a manner similar to...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi602407r
更新日期：2007-03-13 00:00:00
abstract：:Exoenzyme S (ExoS) is a mono-ADP-ribosyltransferase secreted by the opportunistic pathogen Pseudomonas aeruginosa. ExoS requires a eukaryotic factor, the 14-3-3 protein, for enzymatic activity. Here, two aspects of the activation of the ADP-ribosyltransferase activity of ExoS by 14-3-3 proteins are examined. Initial s...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi991019l
更新日期：1999-09-14 00:00:00
abstract：:Iron K-edge X-ray spectroscopy (XANES and EXAFS) was used to study iron coordination in frozen solutions of soybean lipoxygenase-1 (SLO). The intensity of the 1s-->3d pre-edge transition of native iron(II) lipoxygenase is greater than what was found for six-coordinate high-spin iron(II) model complexes, but comparable...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00254a011
更新日期：1994-12-20 00:00:00
abstract：:We have measured three aspects of FtsZ filament dynamics at steady state: rates of GTP hydrolysis, subunit exchange between protofilaments, and disassembly induced by dilution or excess GDP. All three reactions were slowed with an increase in the potassium concentration from 100 to 500 mM, via replacement of potassium...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi8022653
更新日期：2009-07-21 00:00:00
abstract：:A series of truncated forms of His(6)-tagged gp91phox were expressed, solubilized, and purified in the presence of 30 microM FAD. The truncated gp91phox with the longest sequence in the C-terminal region (221-570) (gp91C) showed the highest activity (turnover rate, 0.92) for NADPH diaphorase in the presence of either ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0400249
更新日期：2004-07-27 00:00:00
abstract：:NAD(P) aldehyde dehydrogenases (EC 1.2.1.3) are a family of enzymes that oxidize a wide variety of aldehydes into acid or activated acid compounds. Using site-directed mutagenesis, the essential nucleophilic Cys 149 in the NAD-dependent phosphorylating glyceraldehyde-3-phosphate dehydrogenase from Escherichia coli has...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00164a033
更新日期：1992-12-15 00:00:00
abstract：:Minor leucine tRNA species, tRNA(Leu)4 and tRNA(Leu)5, from Escherichia coli B have been reported to recognize leucine codons UUA and UUG [Goldman, E., Holmes, W. M., and Hatfield, G. W. (1979) J. Mol. Biol. 129, 567-585]. In the present study, these two tRNA(Leu) species were purified from E. coli A19, and the nucleo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi981865g
更新日期：1999-01-05 00:00:00
abstract：:The biosynthesis of osteocalcin (OC), a bone-specific, noncollagenous protein, is stringently regulated during differentiation of the osteoblast phenotype. Glucocorticoids, and also 1,25(OH)2D3, mediate the developmental regulation of OC gene transcription. In this study, we established that the -1097 to +23 promoter ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00093a022
更新日期：1993-10-26 00:00:00
abstract：:Structural analyses of bacterial thymidylate synthases (TSs) implicate a serine residue corresponding to Ser216 in human TS in hydrogen bond networks that are involved in binding of the nucleotide substrate, 2'-deoxyuridylate (dUMP), and that stabilize a beta-bulge in the protein. Utilizing site-directed mutagenesis, ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi972562+
更新日期：1998-05-19 00:00:00
