Ligand stabilization of the beta 2 adrenergic receptor: effect of DTT on receptor conformation monitored by circular dichroism and fluorescence spectroscopy.

abstract：:Escherichia coli DNA photolyase contains 1,5-dihydro-FAD (FADH2) plus 5,10-methenyl-tetrahydrofolate (5,10-CH+-H4folate). Both chromophores are fluorescent, and either can function as a sensitizer in catalysis. At 77 K separate fluorescence emission bands are observed for FADH2 (lambda max = 505 nm, shoulder at 540 nm...

journal_title：Biochemistry

authors： Jordan SP,Jorns MS

更新日期：1988-12-13 00:00:00

abstract：:A series of secreted proteases are included among the virulence factors documented for Staphylococcus aureus. In light of increasing antibiotic resistance of this dangerous human pathogen, these proteases are considered as suitable targets for the development of novel therapeutic strategies. The recent discovery of st...

journal_title：Biochemistry

authors： Dubin G,Krajewski M,Popowicz G,Stec-Niemczyk J,Bochtler M,Potempa J,Dubin A,Holak TA

更新日期：2003-11-25 00:00:00

abstract：:Ribonucleotide reductase (RDPR) from Escherichia coli is composed of two subunits, R1 and R2, both of which are required to catalyze the conversion of nucleotides to deoxynucleotides. This reduction process is accompanied by oxidation of two cysteines within the active site to a disulfide. One of these putative active...

journal_title：Biochemistry

authors： Mao SS,Holler TP,Bollinger JM Jr,Yu GX,Johnston MI,Stubbe J

更新日期：1992-10-13 00:00:00

abstract：:The proximity of skeletal myosin subfragment-1 (S1) to actin, and its orientation with respect to thin filaments of single muscle fibers, were compared in the presence and in the absence of ADP. The proximity was assessed by the efficiency of carbodiimide-induced cross-linking and the orientation by polarization of fl...

journal_title：Biochemistry

authors： Andreev OA,Ushakov DS,Borejdo J

更新日期：1998-12-22 00:00:00

abstract：:We have studied the effect of the binding of ribosomal protein S1 and initiation factor IF3 on the accessibility of nucleotide residues 584-1506 in the small subunit of the Escherichia coli ribosome. Protein S1 strongly decreases RNase V1 attack at G1164, in hairpin 40 of the 3' major domain, and weakly decreases DMS ...

journal_title：Biochemistry

authors： Laughrea M,Tam J

更新日期：1991-12-03 00:00:00

abstract：:Oligodeoxyribonucleotides cross-linked by reductively activated mitomycin C (MC) were prepared and purified for the first time. The cross-linked products were structurally characterized by nucleoside and MC-nucleoside adduct analysis. Optimal conditions were established for the cross-linking reaction, resulting in hig...

journal_title：Biochemistry

authors： Borowy-Borowski H,Lipman R,Chowdary D,Tomasz M

更新日期：1990-03-27 00:00:00

abstract：:The environmentally sensitive fluorescent probes 6-propionyl-2-(N,N-dimethylamino)naphthalene (PRODAN) and 2'-(N,N-dimethylamino)-6-naphthoyl-4-trans-cyclohexanioc acid (DANCA) form complexes with the heme binding site of apohorseradish peroxidase. The dissociation constants of the PRODAN and DANCA complexes were dete...

journal_title：Biochemistry

authors： Lasagna M,Vargas V,Jameson DM,Brunet JE

更新日期：1996-01-23 00:00:00

abstract：:Applications of sensitive new technologies, in particular, two-dimensional NMR spectroscopy, have allowed detection of folded structures in short peptide fragments of proteins in aqueous solution under conditions where native proteins fold. These structures are in rapid dynamic exchange with unfolded states. These obs...

journal_title：Biochemistry

authors： Wright PE,Dyson HJ,Lerner RA

更新日期：1988-09-20 00:00:00

abstract：:To study the role of the N-terminal region of the estrogen receptor (ER) in transcription activation and in DNA binding, we constructed a mutant of the Xenopus laevis ER which lacks amino acids 1-159 (XER160/586). In transient transfections, XER160/586 exhibited < 10% of the activity of wild-type XER on a synthetic pr...

journal_title：Biochemistry

authors： Xing H,Mattick S,Lew D,Shapiro DJ

更新日期：1995-03-28 00:00:00

abstract：:The 35000-Mr beta-adrenergic receptor mRNA binding protein (beta ARB) is induced by beta-adrenergic agonists and binds to G-protein-linked receptor mRNAs that exhibit agonist-induced destabilization. Recently, we identified a 20-nucleotide, AU-rich region in the 3'-untranslated region of the hamster beta 2-adrenergic ...

journal_title：Biochemistry

authors： Tholanikunnel BG,Raymond JR,Malbon CC

更新日期：1999-11-23 00:00:00

abstract：:An endo-beta-galactosidase, which hydrolyzes the internal beta-galactosidic linkages of R----GlcNAc (or GalNAc) beta 1----3Gal beta 1----4GlcNAc (or Glc), was isolated from the culture supernatant of Diplococcus pneumoniae. The enzyme, named endo-beta-galactosidase DII, hydrolyzed linear N-acetyllactosamine repeating ...

journal_title：Biochemistry

authors： Fukuda MN

更新日期：1985-04-23 00:00:00

abstract：:Tularemia is a severe infectious disease in humans caused by the Gram-negative bacterium Francisella tularensis (Ft). Because of its low infectious dose, high mortality rate, and the threat of its large-scale dissemination in weaponized form, development of vaccines and immunotherapeutics against Ft is essential. Ft l...

journal_title：Biochemistry

authors： Wang Q,Shi X,Leymarie N,Madico G,Sharon J,Costello CE,Zaia J

更新日期：2011-12-20 00:00:00

abstract：:The stereochemistry of proton transfer in the isomerization of [4 beta-2H]-5-androstene-3,17- dione (1d) to 4-androstene-3,17-dione (3) catalyzed by 3-oxo-delta 5-steroid isomerase (KSI) has been reinvestigated. In H2O, approximately 65% of the label is retained in the product (3); of this, one-third is at C-4 and two...

journal_title：Biochemistry

authors： Zawrotny ME,Hawkinson DC,Blotny G,Pollack RM

更新日期：1996-05-21 00:00:00

abstract：:Suberyldicholine-induced ion translocation in the millisecond time region in acetylcholine receptor rich membrane vesicles prepared from the electric organ of Electrophorus electricus was investigated in eel Ringer's solution, pH 7.0, 1 degree C. A quench-flow technique with a time resolution of 5 ms was used to measu...

journal_title：Biochemistry

authors： Pasquale EB,Takeyasu K,Udgaonkar JB,Cash DJ,Severski MC,Hess GP

更新日期：1983-12-06 00:00:00

abstract：:The interactions of Pr(III) and Eu(III) with specifically nitrated derivatives of the basic bovine pancreatic trypsin inhibitor have been studied using optical spectroscopy and nuclear magnetic resonance (NMR) at 250 and 270 MHz. Stability constants for proton and metal binding to nitrotyrosines 10 and 21 determined o...

journal_title：Biochemistry

authors： Marinetti TD,Snyder GH,Sykes BD

更新日期：1977-02-22 00:00:00

abstract：:Biogenesis of pyrroloquinoline quinone (PQQ) in Klebsiella pneumoniae requires the expression of six genes (pqqA-F). One of these genes (pqqE) encodes a 43 kDa protein (PqqE) that plays a role in the initial steps in PQQ formation [Veletrop, J. S., et al. (1995) J. Bacteriol. 177, 5088-5098]. PqqE contains two highly ...

journal_title：Biochemistry

authors： Wecksler SR,Stoll S,Tran H,Magnusson OT,Wu SP,King D,Britt RD,Klinman JP

更新日期：2009-10-27 00:00:00

abstract：:Elastin binding proteins from plasma membranes of elastin-producing cells were isolated by affinity chromatography on immobilized elastin peptides. Three proteins of 67, 61, and 55 kDa were released from the elastin resin by guanidine/detergent, soluble elastin peptides, synthetic peptide VGVAPG, or galactoside sugars...

journal_title：Biochemistry

authors： Mecham RP,Hinek A,Entwistle R,Wrenn DS,Griffin GL,Senior RM

更新日期：1989-05-02 00:00:00

abstract：:Vibrational circular dichroism (VCD) and Fourier transform IR (FTIR) were measured for a series of short alanine-based peptides having the general formula Ac-(AAKAA)n-GY-NH2 (n = 1-4) from 5 to 50 degrees C in D2O and at room temperature in both TFE and H2O. In both of these latter solvents, the dominant structural fo...

journal_title：Biochemistry

authors： Yoder G,Pancoska P,Keiderling TA

更新日期：1997-12-09 00:00:00

abstract：:We use laser flash photolysis and time-resolved Raman spectroscopy of CO-bound H93G myoglobin (Mb) mutants to study the influence of the proximal ligand on the CO rebinding kinetics. In H93G mutants, where the proximal linkage with the protein is eliminated and the heme can bind exogenous ligands (e.g., imidazole, 4-b...

journal_title：Biochemistry

authors： Cao W,Ye X,Sjodin T,Christian JF,Demidov AA,Berezhna S,Wang W,Barrick D,Sage JT,Champion PM

更新日期：2004-08-31 00:00:00

abstract：:The individual amide proton exchange rates in Tendamistat at pH 3.0 and 50 degrees C were measured by using two-dimensional 1H nuclear magnetic resonance. Overall, it was found that the distribution of exchange rates along the sequence is dominated by the interstrand hydrogen bonds of the beta-sheet structures. The sl...

journal_title：Biochemistry

authors： Wang QW,Kline AD,Wüthrich K

更新日期：1987-10-06 00:00:00

abstract：:The solution structure of the C-terminal fragment 255-316 of thermolysin has been determined by two-dimensional proton NMR methods. For this disulfide-free fragment there was a previous proposal according to which it would fold into a stable helical structure forming a dimer at concentrations above 0.06 mM. A complete...

journal_title：Biochemistry

authors： Rico M,Jiménez MA,González C,De Filippis V,Fontana A

更新日期：1994-12-13 00:00:00

abstract：:The repair of the endogenous lesion 8-oxo-7,8-dihydrodeoxyguanosine (8-oxodG) was investigated in the nucleotide excision repair mutant xeroderma pigmentosum D (XPD), using human normal or transformed XPD fibroblasts and the Chinese hamster XPD cell line UV5. In vivo repair of 8-oxodG induced by hydrogen peroxide trea...

journal_title：Biochemistry

authors： Cappelli E,Degan P,Thompson LH,Frosina G

更新日期：2000-08-29 00:00:00

abstract：:Among the major obstacles to pharmacological and structural studies of integral membrane proteins (MPs) are their natural scarcity and the difficulty in overproducing them in their native form. MPs can be overexpressed in the non-native state as inclusion bodies, but inducing them to achieve their functional three-dim...

journal_title：Biochemistry

authors： Pocanschi CL,Dahmane T,Gohon Y,Rappaport F,Apell HJ,Kleinschmidt JH,Popot JL

更新日期：2006-11-28 00:00:00

abstract：:Bacterial NO synthase (NOS)-like proteins such as that from Bacillus subtilis (bsNOS) share a high degree of structural homology with the oxygenase domain of mammalian NOSs (mNOSs), but biochemical studies have yet failed to establish that they are specifically capable of producing NO. To better understand the actual ...

journal_title：Biochemistry

authors： Santolini J,Roman M,Stuehr DJ,Mattioli TA

更新日期：2006-02-07 00:00:00

abstract：:Two sites of ligand interaction in acetylcholinesterase (AChE) were first demonstrated in ligand binding studies and later confirmed by crystallography, site-specific mutagenesis, and molecular modeling: an acylation site at the base of the active site gorge and a peripheral site at its mouth. We recently introduced a...

journal_title：Biochemistry

authors： Szegletes T,Mallender WD,Thomas PJ,Rosenberry TL

更新日期：1999-01-05 00:00:00

abstract：:GMP and native DNA were reacted with 7-iodo and 7-fluoro derivatives of N-acetoxy-N-2-acetylaminofluorene. It was shown that the 7-halogeno derivatives react on C-8 of guanine. Furthermore the respective amount of arylamidation (covalent linkage on the C-8 of guanine) and arylation (covalent linkage on 2-NH2 groups of...

journal_title：Biochemistry

authors： Lefèvre JF,Fuchs RP,Daune MP

更新日期：1978-06-27 00:00:00

abstract：:We measure the effects of low concentrations of helix-stabilizing cosolvents, including 2,2,2-trifluoroethanol (TFE), on the thermodynamics and kinetics of folding of the dimeric alpha-helical coiled coil derived from the leucine zipper region of bZIP transcriptional activator GCN4. The change in kinetic behavior upon...

journal_title：Biochemistry

authors： Kentsis A,Sosnick TR

更新日期：1998-10-13 00:00:00

abstract：:G protein-coupled receptor (GPCR) kinases (GRKs) instigate the desensitization of activated GPCRs via phosphorylation that promotes interaction with arrestins, thereby preventing the interaction of GPCRs with heterotrimeric G proteins. A current proposed model of GRK1 activation involves the binding of activated rhodo...

journal_title：Biochemistry

authors： Orban T,Huang CC,Homan KT,Jastrzebska B,Tesmer JJ,Palczewski K

更新日期：2012-04-24 00:00:00

abstract：:MerR, the metalloregulator of the mercury resistance (mer) operon, binds the operator (merO)between -10 and -35 of the merTPCAD promoter (PT) and sequesters RNA polymerase (RNAP) in a closed complex. MerR represses PT until Hg(II) induces it to underwind merO DNA and thus facilitate open complex formation. We used cro...

journal_title：Biochemistry

authors： Kulkarni RD,Summers AO

更新日期：1999-03-16 00:00:00

abstract：:Dehydroquinate synthase has been purified 9000-fold from Escherichia coli K-12 (strain MM294). The synthase is encoded by the aroB gene, which is carried by plasmid pLC29-47 from the Carbon-Clarke library. Construction of an appropriate host bearing pLC29-47 results in a strain that produces 20 times more enzyme than ...

journal_title：Biochemistry

authors： Frost JW,Bender JL,Kadonaga JT,Knowles JR

更新日期：1984-09-11 00:00:00