当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Heme and cysteine microenvironments of tuna apomyoglobin. Evidence of two independent unfolding regions.

Heme and cysteine microenvironments of tuna apomyoglobin. Evidence of two independent unfolding regions.

Abstract：

:The heme and cysteine microenvironments of bluefin tuna apomyoglobin have been investigated by examining the fluorescence properties of two extrinsic chromophores, i.e., ANS and 1,5-AEDANS. 1,5-AEDANS was covalently bound to the single cysteine residue found in the primary structure of tuna apomyoglobin. Recombination experiments with hemin showed that tuna apomyoglobin does not bind 1,5-AEDANS in the same binding site of the heme, although the fluorescence properties of the covalently bound 1,5-AEDANS strongly suggest that the dye is embedded in a rather nonpolar microenvironment. ANS was selected because of its ability to bind the apomyoglobin in the same nonpolar moiety of the heme. Acidification of apoMb--AEDANS to pH 3.0 produced an increase of 1,5-AEDANS fluorescence intensity and a shift of its emission maximum from 475 to 470 nm. In the same pH range apomyoglobin lost its ability to bind ANS. Two independent transitions were observed with increasing concentrations of guanidine. Low guanidine concentration (less than 1.0 M) unfolded the heme binding site as indicated by the disappearance of ANS fluorescence, whereas higher denaturant concentration was required to produce full normalization of 1,5-AEDANS emission spectrum.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Colonna G,Balestrieri C,Bismuto E,Servillo L,Irace G

doi

10.1021/bi00531a002

subject

Has Abstract

pub_date

1982-01-19 00:00:00

pages

212-5

issue

2

eissn

0006-2960

issn

1520-4995

journal_volume

21

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • Structural Control of Nonnative Ligand Binding in Engineered Mutants of Phosphoenolpyruvate Carboxykinase.

    abstract：:Protein engineering to alter recognition underlying ligand binding and activity has enormous potential. Here, ligand binding for Escherichia coli phosphoenolpyruvate carboxykinase (PEPCK), which converts oxaloacetate into CO2 and phosphoenolpyruvate as the first committed step in gluconeogenesis, was engineered to acc...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.8b00963

    authors： Tang HYH,Shin DS,Hura GL,Yang Y,Hu X,Lightstone FC,McGee MD,Padgett HS,Yannone SM,Tainer JA

    更新日期：2018-12-04 00:00:00

  • Beta-tubulin isotypes purified from bovine brain have different relative stabilities.

    abstract：:The highly conserved nature and tissue specificity of the seven vertebrate beta-tubulin isotypes provide circumstantial evidence that functional differences among isotypes may exist in vivo. Compelling evidence from studies of bovine brain beta-isotypes indicated significant conformational and functional differences i...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi972763d

    authors： Schwarz PM,Liggins JR,Ludueña RF

    更新日期：1998-03-31 00:00:00

  • Selective radiolabeling and isolation of the hydrophobic membrane-binding domain of human erythrocyte acetylcholinesterase.

    abstract：:The hydrophobic, membrane-binding domain of purified human erythrocyte acetylcholinesterase was labeled with the photoactivated reagent 3-(trifluoromethyl)-3-(m-[125I]iodophenyl)diazirine. The radiolabel was incorporated when the enzyme was prepared in detergent-free aggregates, in detergent micelles, or in phospholip...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00359a004

    authors： Roberts WL,Rosenberry TL

    更新日期：1986-06-03 00:00:00

  • 24-homologated 1,25-dihydroxyvitamin D3 compounds: separation of calcium and cell differentiation activities.

    abstract：:A series of 24-homologated 1,25-dihydroxyvitamin D3 compounds have been chemically synthesized and studied with regard to their activity in inducing differentiation of human promyelocyte HL-60 cells to monocytes and in calcium mobilizing activity in vitamin D deficient rats. Homologation of 1,25-dihydroxyvitamin D3 or...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00453a026

    authors： Perlman K,Kutner A,Prahl J,Smith C,Inaba M,Schnoes HK,DeLuca HF

    更新日期：1990-01-09 00:00:00

  • Cross-linking of a monomeric 39/34-kDa dispase fragment of von Willebrand factor (Leu-480/Val-481-Gly-718) to the N-terminal region of the alpha-chain of membrane glycoprotein Ib on intact platelets with bis(sulfosuccinimidyl) suberate.

    abstract：:A 39/34-kilodalton (kDa) monomeric dispase fragment of von Willebrand factor (vWF) has been purified by heparin affinity chromatography. Detailed structural analysis of the individual 39- and 34-kDa fragments indicated that they had identical amino acid sequences extending from Leu-480/Val-481 to Gly-718 with an intra...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00447a010

    authors： Andrews RK,Gorman JJ,Booth WJ,Corino GL,Castaldi PA,Berndt MC

    更新日期：1989-10-17 00:00:00

  • Evidence for the amphipathic nature and tilted topology of helices 4 and 5 in the closed state of the colicin E1 channel.

    abstract：:The membrane-bound closed channel structure of helices 4 and 5 (Lys-406-Asp-446) of the colicin E1 channel domain was investigated by using a site-directed fluorescence labeling technique. A bimane probe was covalently attached to a cysteine residue in a series of single-cysteine mutant proteins to scan each helix in ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi801906v

    authors： Ho D,Merrill AR

    更新日期：2009-02-17 00:00:00

  • N-218 MLN64, a protein with StAR-like steroidogenic activity, is folded and cleaved similarly to StAR.

    abstract：:The steroidogenic acute regulatory protein (StAR) facilitates the movement of cholesterol from the outer to inner mitochondrial membrane in adrenal and gonadal cells, fostering steroid biosynthesis. MLN64 is a 445-amino acid protein of unknown function. When 218 amino-terminal residues of MLN-64 are deleted, the resul...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi000911l

    authors： Bose HS,Whittal RM,Huang MC,Baldwin MA,Miller WL

    更新日期：2000-09-26 00:00:00

  • High affinity of the cell-penetrating peptide HIV-1 Tat-PTD for DNA.

    abstract：:During cellular uptake of fluorescently labeled cell-penetrating peptides (CPPs), intense fluorescent signals are commonly observed in the nucleus of the cell, suggesting intracellular CPP relocation and potential binding to the genome of the host. We therefore investigated the interaction of the CPP HIV-1 Tat(47-57) ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi700416h

    authors： Ziegler A,Seelig J

    更新日期：2007-07-10 00:00:00

  • Identification of Filamin A Mechanobinding Partner I: Smoothelin Specifically Interacts with the Filamin A Mechanosensitive Domain 21.

    abstract：:Filamin A (FLNA) is a ubiquitously expressed actin cross-linking protein and a scaffold of numerous binding partners to regulate cell proliferation, migration, and survival. FLNA is a homodimer, and each subunit has an N-terminal actin-binding domain followed by 24 immunoglobulin-like repeats (R). FLNA mediates mechan...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.9b00100

    authors： Wang L,Nakamura F

    更新日期：2019-11-26 00:00:00

  • On the intermediacy of carboxyphosphate in biotin-dependent carboxylations.

    abstract：:In the ATP-dependent carboxylation of biotin that is catalyzed by most biotin-dependent carboxylases, a fundamental mechanistic question is whether the ATP activates bicarbonate (via the formation of carboxyphosphate as an intermediate) or whether the ATP activates biotin (via the formation of O-phosphobiotin). We hav...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00421a009

    authors： Ogita T,Knowles JR

    更新日期：1988-10-18 00:00:00

  • Phase transitions of the purple membranes of Halobacterium halobium.

    abstract：:Purple membranes of Halobacterium halobium were studied by differential scanning calorimetry. No transition was detected at temperatures below 70 degrees C. A small endothermic transition was seen at about 80 degrees C and a larger one at 100 degrees C. The larger transition is the irreversible denaturation of bacteri...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00598a026

    authors： Jackson MB,Sturtevant JM

    更新日期：1978-03-07 00:00:00

  • Dimerization of the extracellular domain of granuloycte-colony stimulating factor receptor by ligand binding: a monovalent ligand induces 2:2 complexes.

    abstract：:Granulocyte-colony stimulating factor (G-CSF) binds to a specific cell surface receptor and induces signals for growth and differentiation in cells of granulocyte hematopoietic lineage. In order to understand how G-CSF binding initiates signals into these cells, we have studied its interactions with the entire extrace...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9525841

    authors： Horan T,Wen J,Narhi L,Parker V,Garcia A,Arakawa T,Philo J

    更新日期：1996-04-16 00:00:00

  • Structure of pseudobactin A214, a siderophore from a bean-deleterious Pseudomonas.

    abstract：:Bean-deleterious Pseudomonas A214 produced the extracellular yellow-green, fluorescent siderophore [microbial iron(III) transport agent] pseudobactin A214 under iron-limiting conditions. Pseudobactin A214 has a molecular formula of C46H64N13O22 and a molecular mass of 1151 g/mol. Pseudobactin A214 contained an N-block...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00367a022

    authors： Buyer JS,Wright JM,Leong J

    更新日期：1986-09-23 00:00:00

  • The axial tyrosinate Fe3+ ligand in protocatechuate 3,4-dioxygenase influences substrate binding and product release: evidence for new reaction cycle intermediates.

    abstract：:The essential active site Fe3+ of protocatechuate 3,4-dioxygenase [3, 4-PCD, subunit structure (alphabetaFe3+)12] is bound by axial ligands, Tyr447 (147beta) and His462 (162beta), and equatorial ligands, Tyr408 (108beta), His460 (160beta), and a solvent OH- (Wat827). Recent X-ray crystallographic studies have shown th...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi972047b

    authors： Frazee RW,Orville AM,Dolbeare KB,Yu H,Ohlendorf DH,Lipscomb JD

    更新日期：1998-02-24 00:00:00

  • Heparin enhances the inhibition of factor Xa by protein C inhibitor in the presence but not in the absence of Ca2+.

    abstract：:Protein C inhibitor (PCI) is a versatile serine protease inhibitor with both pro- and anticoagulant and other properties. Interactions of certain ligands with PCI, including heparin, affect its specificity for proteases. In this study, heparin was found to enhance PCI inhibition of factor Xa up to 42-fold in the prese...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi802138m

    authors： Sun W,Eriksson AS,Schedin-Weiss S

    更新日期：2009-02-10 00:00:00

  • Fusion of phospholipid vesicles induced by muscle glyceraldehyde-3-phosphate dehydrogenase in the absence of calcium.

    abstract：:Ca2+-induced fusion of phospholipid vesicles (phosphatidylcholine/phosphatidic acid, 9:1 mol/mol) prepared by ethanolic injection was followed by five different procedures: resonance energy transfer, light scattering, electron microscopy, intermixing of aqueous content, and gel filtration through Sepharose 4-B. The fi...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00329a015

    authors： Morero RD,Viñals AL,Bloj B,Farías RN

    更新日期：1985-04-09 00:00:00

  • Raman spectroscopy of synthetic antimicrobial frog peptides magainin 2a and PGLa.

    abstract：:Magainin and PGLa are 23- and 21-residue peptides isolated from the skin of the African clawed frog Xenopus laevis. They protect the frog from infection and exhibit a broad-spectrum antimicrobial activity in vitro. The mechanism of this activity involves the interaction of magainin with microbial membranes. We have me...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00470a031

    authors： Williams RW,Starman R,Taylor KM,Gable K,Beeler T,Zasloff M,Covell D

    更新日期：1990-05-08 00:00:00

  • Monoclonal antibody to microtubule-associated STOP protein: affinity purification of neuronal STOP activity and comparison of antigen with activity in neuronal and nonneuronal cell extracts.

    abstract：:Microtubules, ordinarily cold-labile structures, are made entirely resistant to cold temperature by the presence of substoichiometric amounts of STOP (stable tubule only polypeptide), a microtubule-associated protein. We have produced a monoclonal antibody which specifically recognizes a 145-kDa protein previously imp...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00428a064

    authors： Pirollet F,Rauch CT,Job D,Margolis RL

    更新日期：1989-01-24 00:00:00

  • Lipoprotein(a) binds to human platelets and attenuates plasminogen binding and activation.

    abstract：:Lipoprotein(a) [Lp(a)] is a unique lipoprotein consisting of a low-density lipoprotein moiety (LDL) covalently linked to apoprotein(a). Previous work has demonstrated that Lp(a) can compete with plasminogen (PGN) for binding to endothelial and mononuclear cells and can inhibit PGN activation in cell-free systems. We h...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00068a021

    authors： Ezratty A,Simon DI,Loscalzo J

    更新日期：1993-05-04 00:00:00

  • Copper binding to beta-2-microglobulin and its pre-amyloid oligomers.

    abstract：:Beta-2-microglobulin (beta2m) deposits as amyloid fibrils in the musculoskeletal system of patients undergoing long-term dialysis treatment as a result of kidney failure. Previous work has shown that Cu(II) binding causes beta2m to organize into nativelike dimers and tetramers that precede amyloid formation. Cu(II) is...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi901172y

    authors： Srikanth R,Mendoza VL,Bridgewater JD,Zhang G,Vachet RW

    更新日期：2009-10-20 00:00:00

  • Picosecond tryptophan fluorescence of thioredoxin: evidence for discrete species in slow exchange.

    abstract：:The steady-state tryptophan fluorescence and time-resolved tryptophan fluorescence of Escherichia coli thioredoxin, calf thymus thioredoxin, and yeast thioredoxin have been studied. In all proteins, the tryptophan residues undergo strong static and dynamic quenching, probably due to charge-transfer interactions with t...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00434a038

    authors： Mérola F,Rigler R,Holmgren A,Brochon JC

    更新日期：1989-04-18 00:00:00

  • O6-methylguanine in DNA inhibits replication in vitro by human cell extracts.

    abstract：:To study the effects of methylation damage on DNA replication in vitro, the plasmid pSVori containing the SV40 origin of replication was reacted with N-methyl-N-nitrosourea and used as a substrate for SV40 T antigen dependent replication by HeLa cell extracts. The plasmid was methylated with a range of N-methyl-N-nitr...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00212a035

    authors： Ceccotti S,Dogliotti E,Gannon J,Karran P,Bignami M

    更新日期：1993-12-14 00:00:00

  • Binding of Escherichia coli ribonucleic acid polymerase holoenzyme to a bacteriophage T7 promoter-containing fragment: selectivity exists over a wide range of solution conditions.

    abstract：:The selectivity of binding of Escherichia coli RNA polymerase holoenzyme to a promoter-containing fragment of T7 DNA has been investigated over a range of solution conditions by using a double-label nitrocellulose filter binding assay. A 32P-labeled HaeIII restriction fragment of T7 D111 DNA containing the A1 and D pr...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00556a014

    authors： Strauss HS,Burgess RR,Record MT Jr

    更新日期：1980-07-22 00:00:00

  • Stepwise modulation of ATPase activity, nucleotide trapping, and sliding motility of myosin S1 by modification of the thiol region with residues of increasing size.

    abstract：:Rabbit muscle myosin S1 was modified either at SH1 alone or at both SH1 and SH2, using a series of alkylthiolating reagents of increasing size, designed for correlating gradually changing structural disturbances in the thiol region with functional impairments in the myosin head. The reagents were of the type H(CH(2))(...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi991199z

    authors： Tiepold M,Kliche W,Pfannstiel J,Faulstich H

    更新日期：2000-02-15 00:00:00

  • Beta-hydroxydecanoyl thio ester dehydrase does not catalyze a rate-limiting step in Escherichia coli unsaturated fatty acid synthesis.

    abstract：:The intracellular level of beta-hydroxydecanoyl thio ester dehydrase, the product of the fabA gene of Escherichia coli, was increased by isolation of a putative promotor mutant (termed fabAup) or by molecular cloning of the wild-type fabA gene into plasmid pBR322. The fabAup and plasmid-carrying strains overproduced d...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00294a032

    authors： Clark DP,DeMendoza D,Polacco ML,Cronan JE Jr

    更新日期：1983-12-06 00:00:00

  • HO. and DNase I probing of E sigma 70 RNA polymerase--lambda PR promoter open complexes: Mg2+ binding and its structural consequences at the transcription start site.

    abstract：:Chemical and enzymatic probing (footprinting) of the reactivity of the promoter DNA backbone is applied to characterize two binary open complexes RPo1 (-Mg2+) and RPo2 (+Mg2+), formed by Escherichia coli RNA polymerase (E sigma 70) at the lambda PR promoter. We report that HO. detects major differences in backbone rea...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00048a004

    authors： Craig ML,Suh WC,Record MT Jr

    更新日期：1995-12-05 00:00:00

  • 2-Hydroxy-2'-deoxyadenosine 5'-triphosphate enhances A.T --> C.G mutations caused by 8-hydroxy-2'-deoxyguanosine 5'-triphosphate by suppressing its degradation upon replication in a HeLa extract.

    abstract：:The coexistence effects of multiple kinds of oxidized deoxyribonucleotides were examined using an SV40 origin-dependent in vitro replication system with a HeLa extract. Oxidized dGTP and dATP, 8-hydroxy-2'-deoxyguanosine 5'-triphosphate (8-OH-dGTP) and 2-hydroxy-2'-deoxyadenosine 5'-triphosphate (2-OH-dATP), were used...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi062117r

    authors： Satou K,Kasai H,Masutani C,Hanaoka F,Harashima H,Kamiya H

    更新日期：2007-06-05 00:00:00

  • Mutations affecting transition-state stabilization by residues coordinating zinc at the active site of cytidine deaminase.

    abstract：:Cytidine deaminase from Escherichia coli contains 1 mol of tightly bound zinc per enzyme subunit (Yang, C., Carlow, D., Wolfenden, R., & Short, S.A. (1992) Biochemistry 31, 4168-4174). When the metal liganding residues Cys-129 and Cys-132 were replaced by Ala, and His-102 was replaced by Ala, Asn, or Gln, deaminase ac...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00187a012

    authors： Smith AA,Carlow DC,Wolfenden R,Short SA

    更新日期：1994-05-31 00:00:00

  • Kinetics of polymerization of a fluoresceinated derivative of complement protein C9 by the membrane-bound complex of complement proteins C5b-8.

    abstract：:The fluorescence self-quenching by energy transfer of FITC-C9, a fluoresceinated derivative of human complement protein C9 [Sims, P.J. (1984) Biochemistry (preceding paper in this issue)], has been used to monitor the kinetics of C9 polymerization induced by the membrane-associated complex of complement proteins C5b-8...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00309a021

    authors： Sims PJ,Wiedmer T

    更新日期：1984-07-03 00:00:00

  • Importance of loops of mitochondrial ADP/ATP carrier for its transport activity deduced from reactivities of its cysteine residues with the sulfhydryl reagent eosin-5-maleimide.

    abstract：:The effects of various compounds such as the transport substrate ADP and the transport inhibitors carboxyatractyloside (CATR) and bongkrekic acid (BKA) on the labeling of cysteine residues in the ADP/ATP carrier of bovine heart submitochondrial particles by the SH reagent eosin-5-maleimide (EMA) were studied. Of the f...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00198a019

    authors： Majima E,Shinohara Y,Yamaguchi N,Hong YM,Terada H

    更新日期：1994-08-16 00:00:00