HlyC, the internal protein acyltransferase that activates hemolysin toxin: role of conserved histidine, serine, and cysteine residues in enzymatic activity as probed by chemical modification and site-directed mutagenesis.

abstract：:Depletion of functional Ca2+ from photosystem (PS) II membranes impairs O2 evolution. Redox properties of the Mn cluster as probed by thermoluminescence were modified differently in Ca(2+)-depleted PSII depending on the procedure for Ca2+ extraction. Ca2+ depletion by low-pH treatment gave rise to an abnormally modifi...

journal_title：Biochemistry

authors： Ono T,Izawa S,Inoue Y

更新日期：1992-08-25 00:00:00

abstract：:The binding of Sp1 transcription factor to DNA is considered a potential target for small ligands designed to interfere with gene transcription. We attempted to distinguish the direct inhibition of the Sp1-binding to DNA in vivo (cell culture) from more indirect effects due to the network of pathways that modulate cel...

journal_title：Biochemistry

authors： Mansilla S,Priebe W,Portugal J

更新日期：2004-06-15 00:00:00

abstract：:A major challenge to the study of protein folding is the fact that intermediate states along the reaction pathway are generally unstable and thus difficult to observe. Recently developed NMR relaxation dispersion experiments present an avenue to accessing such states, providing kinetic, thermodynamic, and structural i...

journal_title：Biochemistry

authors： Mittermaier A,Korzhnev DM,Kay LE

更新日期：2005-11-29 00:00:00

abstract：:Many important human pathogens rely on one or more type three secretion systems (T3SSs) to inject bacterial effector proteins directly into the host cell cytoplasm. Secretion of protein through the needlelike type three secretion apparatus (T3SA) is essential for pathogen virulence and relies on a highly conserved ATP...

journal_title：Biochemistry

authors： Case HB,Mattock DS,Dickenson NE

更新日期：2018-12-18 00:00:00

abstract：:The sigmoidal dependence of activity on substrate concentration exhibited by the regulatory enzyme aspartate transcarbamoylase (ATCase) of Escherichia coli is generally attributed to a ligand-promoted change in the quaternary structure of the enzyme. Although a global conformational change in ATCase upon the binding o...

journal_title：Biochemistry

authors： Eisenstein E,Markby DW,Schachman HK

更新日期：1990-04-17 00:00:00

abstract：:The membrane topology of the human apical sodium-dependent bile acid transporter (hASBT) remains unresolved. Whereas N-glycosylation analysis favors a 7 transmembrane (TM) model, membrane insertion scanning supports a 9TM topology. In order to resolve this controversy, we used dual label epitope insertion to systemati...

journal_title：Biochemistry

authors： Banerjee A,Swaan PW

更新日期：2006-01-24 00:00:00

abstract：:The dynamic behavior of the rRNA A-site plays an important functional role. We have employed femtosecond time-resolved spectroscopy to investigate the nature of the conformational dynamics. In the drug-free state, the A-site samples multiple distinct conformations. Drug binding shifts the population distribution in a ...

journal_title：Biochemistry

authors： Lu J,Zhao L,Xia A,Xia T,Qi X

更新日期：2013-03-12 00:00:00

abstract：:Heme catabolism is an important biochemical process that many bacterial pathogens utilize to acquire iron. However, tetrapyrrole catabolites can be reactive and often require further processing for transport out of the cell or conversion to another useful cofactor. In previous work, we presented in vitro evidence of a...

journal_title：Biochemistry

authors： LaMattina JW,Delrossi M,Uy KG,Keul ND,Nix DB,Neelam AR,Lanzilotta WN

更新日期：2017-02-14 00:00:00

abstract：:In a previous report, the cDNA for human proteinase inhibitor 8 (PI8) was first identified, isolated, and subcloned into a mammalian expression vector and expressed in baby hamster kidney cells. Initial studies indicated that PI8 was able to inhibit the amidolytic activity of trypsin and form an SDS-stable approximate...

journal_title：Biochemistry

authors： Dahlen JR,Foster DC,Kisiel W

更新日期：1997-12-02 00:00:00

abstract：:Triglyceride analogues were synthesized in which one of the primary acyl ester functions has been replaced by an alkyl group and the secondary acyl ester bond has been replaced by an acyl amino bond. The chain length at either position was varied, and both (R)- and (S)-enantiomers of each compound were synthesized. Th...

journal_title：Biochemistry

authors： Mannesse ML,Cox RC,Koops BC,Verheij HM,de Haas GH,Egmond MR,van der Hijden HT,de Vlieg J

更新日期：1995-05-16 00:00:00

abstract：:Lactose repressor protein, regulator of lac enzyme expression in Escherichia coli, maintains its structure and function at extremely low protein concentrations (<10(-)12 M). To examine the unfolding and dissociation of this tetrameric protein, structural transitions in the presence of varying concentrations of urea we...

journal_title：Biochemistry

authors： Barry JK,Matthews KS

更新日期：1999-05-18 00:00:00

abstract：:The Hsp70-class molecular chaperone HscA interacts specifically with a conserved (99)LPPVK(103) motif of the iron-sulfur cluster scaffold protein IscU. We used a cellulose-bound peptide array to perform single-site saturation substitution of peptide residues corresponding to Glu(98)-Ile(104) of IscU to determine posit...

journal_title：Biochemistry

authors： Tapley TL,Cupp-Vickery JR,Vickery LE

更新日期：2006-07-04 00:00:00

abstract：:DNA containing short sequences of the form (dA)n.(dT)n can exhibit pronounced degrees of stable curvature of the helix axis, provided that these homooligomeric stretches are approximately in phase with the helix repeat. However, the precise origin of this effect is unknown. We have observed that pyrimidine 5-methyl gr...

journal_title：Biochemistry

authors： Hagerman PJ

更新日期：1990-02-27 00:00:00

abstract：:Thiolases are CoA-dependent enzymes which catalyze the formation of a carbon-carbon bond in a Claisen condensation step and its reverse reaction via a thiolytic degradation mechanism. Mitochondrial acetoacetyl-coenzyme A (CoA) thiolase (T2) is important in the pathways for the synthesis and degradation of ketone bodie...

journal_title：Biochemistry

authors： Haapalainen AM,Meriläinen G,Pirilä PL,Kondo N,Fukao T,Wierenga RK

更新日期：2007-04-10 00:00:00

abstract：:Adduct-induced DNA damage can affect transcription efficiency and DNA replication and repair. We previously investigated the effects of the 3'-next flanking base (G*CT vs G*CA; G*, FABP, N-(2'-deoxyguanosin-8-yl)-4'-fluoro-4-aminobiphenyl; FAF, N-(2'-deoxyguanosin-8-yl)-7-fluoro-2-aminofluorene) on the conformation of...

journal_title：Biochemistry

authors： Jain V,Vaidyanathan VG,Patnaik S,Gopal S,Cho BP

更新日期：2014-06-24 00:00:00

abstract：:X-ray absorption spectra are reported for the multi-Cu oxidase Rhus vernicifera laccase in oxidized and fully reduced forms and for laccase from which the type 2 Cu has been depleted (T2D). The structure of the Cu K edge for both preparations shows the presence of CuII and CuI in the oxidized and reduced states, respe...

journal_title：Biochemistry

authors： Woolery GL,Powers L,Peisach J,Spiro TG

更新日期：1984-07-17 00:00:00

abstract：:Electronic absorption, EPR, and resonance Raman spectroscopies revealed that CooA, the CO-sensing transcriptional regulator from Rhodospirillum rubrum, reacts with NO to form a five-coordinate NO-heme. NO must therefore displace both of the heme ligands from six-coordinate, low-spin Fe(II)CooA in forming five-coordina...

journal_title：Biochemistry

authors： Reynolds MF,Parks RB,Burstyn JN,Shelver D,Thorsteinsson MV,Kerby RL,Roberts GP,Vogel KM,Spiro TG

更新日期：2000-01-18 00:00:00

abstract：:Quorum sensing, the ability of bacteria to sense their own population density through the synthesis and detection of small molecule signals, has received a great deal of attention in recent years. Acyl homoserine lactones (AHLs) are a major class of quorum sensing signaling molecules. In nature, some bacteria that do ...

journal_title：Biochemistry

authors： Chowdhary PK,Keshavan N,Nguyen HQ,Peterson JA,González JE,Haines DC

更新日期：2007-12-18 00:00:00

abstract：:The kinetics of ATP-induced Ca2+ uptake by vesicular dispersions of sarcoplasmic reticulum were determined with a time resolution of about 10 ms, depending on the temperature. Ca2+ uptake was initiated by the addition of ATP through the flash photolysis of P3-1-(2-nitrophenyl)-ethyl adenosine 5'-triphosphate utilizing...

journal_title：Biochemistry

authors： Pierce DH,Scarpa A,Topp MR,Blasie JK

更新日期：1983-11-08 00:00:00

abstract：:P700, the primary electron donor of photosystem I is an asymmetric dimer made of one molecule of chlorophyll a' (P(A)) and one of chlorophyll a (P(B)). While the carbonyl groups of P(A) are involved in hydrogen-bonding interactions with several surrounding amino acid side chains and a water molecule, P(B) does not eng...

journal_title：Biochemistry

authors： Pantelidou M,Chitnis PR,Breton J

更新日期：2004-07-06 00:00:00

abstract：:The solution structures of peptide antigens from the receptor binding domains of Pseudomonas aeruginosa strains PAO and KB7 have been determined using two-dimensional 1H NMR techniques. Ensembles of solution conformations for the trans forms of these 17-residue disulfide-bridged peptides have been generated using a si...

journal_title：Biochemistry

authors： Campbell AP,McInnes C,Hodges RS,Sykes BD

更新日期：1995-12-19 00:00:00

abstract：:Nitrophorins 1-4 (NP1-4) are ferriheme proteins from the blood-sucking insect Rhodnius prolixus that transport nitric oxide (NO) to the victim, sequester histamine, and inhibit blood coagulation. Here, we report kinetic and thermodynamic analyses for ligand binding by all four proteins and their reduction potentials. ...

journal_title：Biochemistry

authors： Andersen JF,Ding XD,Balfour C,Shokhireva TK,Champagne DE,Walker FA,Montfort WR

更新日期：2000-08-22 00:00:00

abstract：:The thermodynamic and activation parameters for the reduction of delta 1-pyrroline-2-carboxylic acid (an alpha-imino acid) by reduced nicotinamide adenine dinucleotide phosphate (NADPH) are compared with those for the reduction of the same imino acid by the glutamate dehydrogenase-NADPH complex. The enthalpies of acti...

journal_title：Biochemistry

authors： Srinivasan R,Fisher HF

更新日期：1985-09-24 00:00:00

abstract：:d-2-Hydroxyglutarate dehydrogenase from Pseudomonas aeruginosa PAO1 (PaD2HGDH) catalyzes the oxidation of d-2-hydroxyglutarate to 2-ketoglutarate, which is a necessary step in the serine biosynthetic pathway. The dependence of P. aeruginosa on PaD2HGDH makes the enzyme a potential therapeutic target against P. aerugin...

journal_title：Biochemistry

authors： Quaye JA,Gadda G

更新日期：2020-12-29 00:00:00

abstract：:The fluorescence characteristics of brevin and the effects of Ca2+ on the protein conformation were fully investigated. Brevin contains 18 tryptophans and 27 tyrosines. Analysis of the fluorescence spectra and the accessibility to quenching molecules indicate that the emitting tryptophans are located in a hydrophobic ...

journal_title：Biochemistry

authors： Kilhoffer MC,Gérard D

更新日期：1985-09-24 00:00:00

abstract：:The increase of the proton motive force mediated by bacteriorhodopsin is compared to the intracellular ATP concentration under steady-state illumination. The membrane potential was measured via the accumulation of the lipophilic ion [14C]triphenylmethylphosphonium and the pH gradient via the accumulation of the weak a...

journal_title：Biochemistry

authors： Michel H,Oesterhelt D

更新日期：1980-09-30 00:00:00

abstract：:A variety of chronic inflammatory conditions are associated with an increased risk for the development of cancer. Because of the numerous links between DNA oxidative damage and carcinogenesis, a potential role for leukocyte-generated oxidants in these processes has been suggested. In the present study, we demonstrate ...

journal_title：Biochemistry

authors： Shen Z,Wu W,Hazen SL

更新日期：2000-05-09 00:00:00

abstract：:Integrin αIIbβ3, a transmembrane heterodimer, mediates platelet aggregation when it switches from an inactive to an active ligand-binding conformation following platelet stimulation. Central to regulating αIIbβ3 activity is the interaction between the αIIb and β3 extracellular stalks, which form a tight heterodimer in...

journal_title：Biochemistry

authors： Tan SK,Fong KP,Polizzi NF,Sternisha A,Slusky JSG,Yoon K,DeGrado WF,Bennett JS

更新日期：2019-07-30 00:00:00

abstract：:The unfolding and refolding of alpha-helical proteins has been extensively studied, demonstrating formation of intermediate structures which retain the native-like alpha-helix but lack the tertiary structure. Studies on the folding of proteins consisting primarily of beta-sheet are interesting since, unlike the alpha-...

journal_title：Biochemistry

authors： Narhi LO,Philo JS,Li T,Zhang M,Samal B,Arakawa T

更新日期：1996-09-03 00:00:00

abstract：:Rat organic anion transporting protein 1a1 (oatp1a1), a hepatocyte basolateral plasma membrane protein, mediates transport of various amphipathic compounds. Our previous studies indicated that serine phosphorylation of a single tryptic peptide inhibits its transport activity without changing its cell surface content. ...

journal_title：Biochemistry

authors： Xiao Y,Nieves E,Angeletti RH,Orr GA,Wolkoff AW

更新日期：2006-03-14 00:00:00