Abstract：
:A series of promoters with nine base-pair substitutions in the spacer DNA separating the -10 and -35 regions was used to demonstrate that Escherichia coli RNA polymerase is sensitive to events affecting the spacer DNA--a region not directly contacted by the enzyme. The drugs distamycin A and netropsin specifically enhanced the rate of functional complex formation at a promoter bearing a substitution of nonalternating A-T base pairs. The effect is exerted at an early step in the RNA polymerase-promoter interaction. We hypothesize that a drug-induced structural alteration in the spacer DNA occurs, similar to that normally resulting from RNA polymerase binding. These findings are relevant to an understanding of potential mechanisms of transcription activation.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Bruzik JP,Auble DT,deHaseth PLdoi
10.1021/bi00377a040subject
Has Abstractpub_date
1987-02-10 00:00:00pages
950-6issue
3eissn
0006-2960issn
1520-4995journal_volume
26pub_type
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