Abstract:
:The formation and excision of 313-nm light-induced cyclobutane-type pyrimidine photodimers were determined in confluent cultures of human fibroblasts. A new method was developed for the resolution and determination of cytosine-thymine (CT) and thymine-thymine dimers (TT) by using sodium borohydride reduction and high-pressure liquid chromatography. This assay can detect as little as 1.8 TT or 5.6 CT per 10(8) daltons, levels induced in monolayers of human skin fibroblasts by doses of 1 and 2 kJ m-2 of 313-nm light, respectively. CT formation was 20% more efficient than TT formation in the physiological dose range of 2.25-15 k m-2 at 37 degrees C. Normal fibroblasts removed 61% TT within the first 8 h of incubation following a dose of 5.5 kJ m-2. CT was removed approximately twice as efficiently as TT during the same time period following exposure to 10 kJ m-2. The lack of removal of CT as well as TT observed in xeroderma pigmentosum fibroblasts indicates that the repair deficiency in these cells affects the repair of both classes of dimers.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Niggli HJ,Cerutti PAdoi
10.1021/bi00275a011subject
Has Abstractpub_date
1983-03-15 00:00:00pages
1390-5issue
6eissn
0006-2960issn
1520-4995journal_volume
22pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::The stereochemical problem involving a pro-pro-prochiral phosphorus center, the hydrolysis of adenosine 5'-monophosphate to adenosine and inorganic phosphate catalyzed by the venom 5'-nucleotidase, has been studied by use of chiral [16O, 17O, 18O]thiophosphates (Psi). (Rp)- and (Sp)-[alpha-18O1]Adenosine 5'-thiophosph...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00564a025
更新日期:1980-11-11 00:00:00
abstract::S-(4-Bromo-2,3-dioxobutyl)glutathione (S-BDB-G), a reactive analogue of glutathione, has been synthesized and characterized by UV spectroscopy and thin-layer chromatography, as well as by bromide and primary amine analysis. Incubation of S-BDB-G (200 microM) with the 4-4 isoenzyme of rat liver glutathione S-transferas...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00111a006
更新日期:1991-11-26 00:00:00
abstract::Factor XIIIa (a2') is a homodimeric transglutaminase that is formed via limited alpha-thrombin-catalyzed proteolysis of the platelet (a2) or plasma (a2b2) factor XIII zymogen in a reaction that results in proteolytic removal of a 37-aminoacyl residue peptide from the N-terminus of the a chains and exposure of the acti...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00117a017
更新日期:1992-01-21 00:00:00
abstract::An important subject for elucidating membrane protein (MP) folding is how transmembrane helices (TMHs) insert into and dissociate from membranes. We investigated helix dissociation kinetics and insertion topology by means of intervesicular transfer of the fluorophore-labeled completely hydrophobic model transmembrane ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi026191v
更新日期:2002-10-15 00:00:00
abstract::While the critical role of the bacterial luciferase alpha subunit in catalysis has been amply documented, the beta subunit was only known to be involved in thermal stability and substrate binding. Two conserved histidyl residues at position 81 and 82 of the beta subunit of Vibrio harveyi luciferase were each mutated t...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00206a023
更新日期:1994-10-11 00:00:00
abstract::Kinetic study of a series of compounds containing the thrombin-directed peptide D-Phe-ProboroArg-OH had indicated that the structure of the N-terminal blocking group may be correlated with binding [Kettner, C., Mersinger, L., & Knabb, R. (1990) J. Biol. Chem. 265, 18289-18297]. In order to further study this phenomeno...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi952164b
更新日期:1996-07-30 00:00:00
abstract::Smooth muscle cells maintain filaments of actin and myosin in the presence of ATP, although dephosphorylated myosin filaments and actin-myosin interactions are unstable under those conditions in vitro. Several proteins that stabilize myosin filaments and that stabilize actin-myosin interactions have been identified. F...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi401013g
更新日期:2013-10-29 00:00:00
abstract::Continuum methods were used to calculate the electrostatic contributions of charged and polar side chains to the overall stability of a small 41-residue helical protein, the peripheral subunit-binding domain. The results of these calculations suggest several residues that are destabilizing, relative to hydrophobic iso...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi992091m
更新日期:2000-02-08 00:00:00
abstract::The ErbB family of tyrosine kinase receptors is a key element in preserving cell growth homeostasis. This family is comprised of four single-transmembrane domain proteins designated ErbB-1-4. Ligand binding initiates dimerization followed by tyrosine phosphorylation and signaling, which when uncontrolled lead to cance...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.6b00450
更新日期:2016-10-04 00:00:00
abstract::The electron spin resonance (ESR) spectra from spin-labeled phospholipids in recombinants of myelin proteolipid apoprotein with dimyristoylphosphatidylcholine have been simulated with the exchanged-coupled Bloch equations to obtain values for both the fraction of motionally restricted lipids and the exchange rate betw...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00401a009
更新日期:1988-01-12 00:00:00
abstract::To study fibronectin (FN) conformation and assembly, we generated several deletion mutants: FNΔI1-5, FNΔIII1-3, FNΔIII4-8, and FNΔIII11-14. A monomeric form, FNmono, which lacked the C-terminal dimerization region, was also created. FNtnA-D was generated by swapping FNIII domains 1-8 in FNΔIII11-14 with seven FNIII do...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00589
更新日期:2017-08-29 00:00:00
abstract::The hydrogen exchange behavior of the N-terminal helical segment in horse heart cytochrome c was studied in both the reduced and the oxidized forms by use of two-dimensional nuclear magnetic resonance methods. The amide protons of the first six residues are not H bonded and exchange rapidly with solvent protons. The m...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00353a025
更新日期:1986-03-11 00:00:00
abstract::Dihydrofolate and dihydropteroylpolyglutamates inhibit pig liver methylenetetrahydrofolate reductase. In all cases the inhibition is linearly competitive with respect to methylenetetrahydrofolate. The Ki values decrease with each additional glutamyl residue from one to six, from a value of 6.5 microM for dihydrofolate...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00551a005
更新日期:1980-05-13 00:00:00
abstract::Beta-amylase (EC 3.2.1.2) is starch-hydrolyzing exo-type enzyme that can catalyze the successive liberation of beta-maltose from the nonreducing ends of alpha-1,4-linked glucopyranosyl polymers. There is a well-known phenomenon called multiple or repetitive attack where the enzyme releases several maltose molecules in...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061605w
更新日期:2007-01-23 00:00:00
abstract::Stopped-flow experiments obtained in the pre-steady-state time scale of the NAD-malic enzyme reaction exhibit a lag prior to the attainment of steady state. Previous results from isotope effect studies in which the deuterium isotope effect on Vmax decreases to a value of 1 at low pH have been interpreted as suggesting...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00059a007
更新日期:1993-03-02 00:00:00
abstract::Hepatic microsomal cytochrome P-450 from phenobarbital-pretreated rats is destroyed by propyne in a reduced nicotinamide adenine dinucleotide dependent process which also results in vivo in the accumulation of an abnormal green porphyrin. The green porphyrin has been identified by its electronic absorption, mass spect...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00528a033
更新日期:1981-12-08 00:00:00
abstract::The electric organ of the ray, Torpedo ocellata, can serve as a source for both the acetylcholine (ACh) receptor and its ionic channel. The two entities were identified by their specific binding of [3H]ACh and [3H]perhydrohistrionicotoxin ([3H]H12-HTX), respectively. Binding of [3H]H12-HTX was inhibited by certain dru...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00618a023
更新日期:1978-12-12 00:00:00
abstract::Phe-tRNAPhe species modified on the 3'-terminal ribose residue were investigated for their ability to participate in individual steps of the elongation cycle using eukaryotic ribosomes from reticulocytes. None of the Phe-tRNAs used, namely Phe-tRNAPhe-C-C-3'dA, Phe-tRNAPhe-C-C-3'-NH2A, and Phe-tRNAPhe-C-C-Aoxi-red, ca...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00661a035
更新日期:1976-08-10 00:00:00
abstract::The zinc metalloenzyme protein farnesyltransferase (FTase) catalyzes the alkylation of a cysteine residue of protein substrates with a 15 carbon farnesyl group. We have developed fluorescence assays to directly measure the affinity of the enzyme for peptide and protein (Ras) substrates. A peptide corresponding to the ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi981525v
更新日期:1998-11-03 00:00:00
abstract::In this report we provide biochemical evidence that a highly purified synaptic plasma membrane fraction derived from rat brain, after intraventricular injection of 35S-labeled sodium sulfate, is enriched in a number of large sulfated glycoproteins compared with a purified myelin fraction studied concurrently. A fracti...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00670a005
更新日期:1976-12-14 00:00:00
abstract::G-Actin is a globular protein (Mr 42 300) known to have three cysteine residues that are at least partially exposed and chemically reactive (Cys-10, -284, and -374). When G-actin was reacted with 3-bromo-1,1,1-trifluoropropanone, three resolvable 19F resonances were observed in the 19F NMR spectrum. This fluorinated G...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00356a050
更新日期:1986-04-22 00:00:00
abstract::The (1)H NMR resonances of the heme substituents of the low-spin Fe(III) form of nitrophorin 2, as its complexes with N-methylimidazole (NP2-NMeIm) and imidazole (NP2-ImH), have been assigned by a combination of (1)H homonuclear two-dimensional NMR techniques and (1)H-(13)C HMQC. Complete assignment of the proton and ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi026765w
更新日期:2003-01-28 00:00:00
abstract::Endogenous protein kinase inhibitors are essential for a wide range of physiological functions. These endogenous inhibitors may mimic peptide substrates as in the case of the heat-stable protein kinase inhibitor (PKI), or they may mimic nucleotide triphosphates. Natural product inhibitors, endogenous to the unique org...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9820659
更新日期:1999-02-23 00:00:00
abstract::The thermal unfolding of rabbit skeletal heavy meromyosin (HMM), myosin subfragment 1, and subfragment 2 has been studied by differential scanning calorimetry (DSC). Two distinct endotherms are observed in the DSC scan of heavy meromyosin. The first endotherm, with a Tm of 41 degrees C at pH 7.9 in 0.1 M KCl, is assig...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00462a018
更新日期:1990-03-13 00:00:00
abstract::Metal homeostasis and resistance in bacteria is maintained by a panel of metal-sensing transcriptional regulators that collectively control transition metal availability and mediate resistance to heavy metal xenobiotics, including As(III), Cd(II), Pb(II), and Hg(II). The ArsR family constitutes a superfamily of metal ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi100490u
更新日期:2010-08-10 00:00:00
abstract::Limited digestion of polysomal RNA with pancreatic ribonuclease releases a structure consisting of poly(A) associated with other polyribonucleotide sequences. This complex can be purified by oligo(dT)-cellulose chromatography. Heating for formamide treatment causes the dissociation of fragments free of poly(A) from th...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00686a024
更新日期:1975-07-29 00:00:00
abstract::The neonatal Fc receptor (FcRn) binds maternal immunoglobulin G (IgG) during the acquisition of passive immunity by the fetus or newborn. In adult mammals, FcRn also binds IgG and returns it to the bloodstream, thus protecting IgG from a default degradative pathway. Biosensor assays have been used to characterize the ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi970841r
更新日期:1997-08-05 00:00:00
abstract::One- and two-dimensional NMR experiments were carried out on a decamer, d-(CGCTTTTCGC).d(GCGAAAAGCG), and on the same sequence with the addition of an unpaired thymidine, d(CGCTTTTCGC).d(GCGAATAAGCG), which will be referred to as the T-bulge decamer. Evidence from one-dimensional NOE experiments on the exchangeable pr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00489a047
更新日期:1990-09-18 00:00:00
abstract::The effects of urea on apomyoglobin solubility have been investigated. Apomyoglobin precipitation was found to be a thermodynamically reversible process independent of the pathway of aggregation. A liquid-solid phase diagram was constructed for the precipitation of apomyoglobin as a function of urea and protein concen...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00066a006
更新日期:1993-04-20 00:00:00
abstract::The solution structure of an alpha-insect toxin from Buthus martensii Karsch, BmKalphaIT01, has been determined by two-dimensional NMR spectroscopy and molecular modeling techniques. Combining the sequence homology comparison and toxicity bioassays, BmKalphaIT01 has been suggested to be a natural mutant of alpha-insec...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi7006788
更新日期:2007-10-09 00:00:00