Chloroacetaldehyde-treated ribo- and deoxyribopolynucleotides. 1. Reaction products.

abstract：:Transmembrane chemoreceptors, also known as methyl-accepting chemotaxis proteins (MCPs), translate extracellular signals into intracellular responses in the bacterial chemotaxis system. MCP ligand binding domains control the activity of the CheA kinase, situated approximately 200 A away, across the cytoplasmic membran...

journal_title：Biochemistry

authors： Pollard AM,Bilwes AM,Crane BR

更新日期：2009-03-10 00:00:00

abstract：:The beta- and delta-subunits of the nicotinic acetylcholine receptor from Torpedo californica were covalently photolabeled at the synaptic surface with the ATP photoaffinity analogue [alpha-32P]-8-azido-ATP. The specificity of labeling for nucleotide binding sites was demonstrated by the saturation of labeling with in...

journal_title：Biochemistry

authors： Carlson BJ,Raftery MA

更新日期：1993-07-27 00:00:00

abstract：:A series of secreted proteases are included among the virulence factors documented for Staphylococcus aureus. In light of increasing antibiotic resistance of this dangerous human pathogen, these proteases are considered as suitable targets for the development of novel therapeutic strategies. The recent discovery of st...

journal_title：Biochemistry

authors： Dubin G,Krajewski M,Popowicz G,Stec-Niemczyk J,Bochtler M,Potempa J,Dubin A,Holak TA

更新日期：2003-11-25 00:00:00

abstract：:The carboxy-terminal domain of the alpha subunit of Escherichia coli RNA polymerase, which is connected with the core part of RNA polymerase through a long flexible linker, plays decisive roles in transcription activation by directly interacting with a large number of transcription factors and upstream (UP) element DN...

journal_title：Biochemistry

authors： Fujita N,Endo S,Ishihama A

更新日期：2000-05-23 00:00:00

abstract：:Cyanobacteriochromes (CBCRs) are photosensory proteins related to the red/far-red phytochromes. Like phytochromes, CBCRs use linear tetrapyrrole (bilin) chromophores covalently attached via a thioether linkage to a conserved Cys residue also found in plant and cyanobacterial phytochromes. Unlike almost all phytochrome...

journal_title：Biochemistry

authors： Rockwell NC,Martin SS,Lagarias JC

更新日期：2012-05-01 00:00:00

abstract：:Hypochlorous acid (HOCl) is a powerful oxidant generated from H(2)O(2) and Cl(-) by the heme enzyme myeloperoxidase, which is released from activated leukocytes. HOCl possesses potent antibacterial properties, but excessive production can lead to host tissue damage that is implicated in a wide range of human diseases ...

journal_title：Biochemistry

authors： Pattison DI,Davies MJ

更新日期：2006-07-04 00:00:00

abstract：:Steroid receptors define a family of ligand-activated transcription factors. Recent work has demonstrated that the receptors regulate distinct but overlapping gene networks, yet the mechanisms by which they do so remain unclear. We previously determined the microscopic binding energetics for progesterone receptor (PR)...

journal_title：Biochemistry

authors： Moody AD,Miura MT,Connaghan KD,Bain DL

更新日期：2012-01-24 00:00:00

abstract：:Nitric oxide is produced in mammals by the nitric oxide synthase (NOS) isoforms at a catalytic site comprising a heme associated with a biopterin cofactor. Through genome sequencing, proteins that are highly homologous to the oxygenase domain of NOSs have been identified, in particular in bacteria. The active site is ...

journal_title：Biochemistry

authors： Weisslocker-Schaetzel M,Lembrouk M,Santolini J,Dorlet P

更新日期：2017-02-07 00:00:00

abstract：:A simple and rapid procedure is described for purification of P-glycoprotein (Pgp) from a multidrug-resistant Chinese hamster ovary cell line (CR1R12) in which the plasma membranes are highly enriched in Pgp (Al-Shawi, M.K., Senior A.E. (1993) J. Biol. Chem, 268, 4197-4206). The procedure consisted of octylglucoside s...

journal_title：Biochemistry

authors： Urbatsch IL,al-Shawi MK,Senior AE

更新日期：1994-06-14 00:00:00

abstract：:The conformational states of N-acylalanine dithio esters, involving rotational isomers about the RC(=O)NH--CH(CH3) and NHCH(CH3)--C(=S) bonds, are defined and compared to those of N-acylglycine dithio esters. The structure of N-(p-nitrobenzoyl)-DL-alanine ethyl dithio ester has been determined by X-ray crystallographi...

journal_title：Biochemistry

authors： Lee H,Angus RH,Storer AC,Varughese KI,Carey PR

更新日期：1988-01-12 00:00:00

abstract：:p-(Chloromercuri)benzoic acid inhibited the catalytic activity of Bacillus stearothermophilus farnesyl diphosphate synthase (FPP synthase), which contains only two cysteine residues at positions 73 and 289. In order to explore the role of the cysteine residues, either or both of them were replaced with phenylalanine o...

journal_title：Biochemistry

authors： Koyama T,Obata S,Saito K,Takeshita-Koike A,Ogura K

更新日期：1994-10-25 00:00:00

abstract：:CaATPase from rabbit sarcoplasmic reticulum (SR) has been isolated, purified, and reconstituted into unilamellar vesicles with 1,2-dipentadecanoylphosphatidylcholine (DPePC) and acyl-chain-perdeuterated 1,2-dipalmitoylphosphatidylcholine (DPPC-d62). For total lipid:protein mole ratios between 25 and 70, a constant "ha...

journal_title：Biochemistry

authors： Senak L,Mendelsohn R

更新日期：1993-06-22 00:00:00

abstract：:Among the molecular strategies bacteria have set up to quickly match their transcriptional program to new environments, changes in sequence-mediated DNA curvature play a crucial role. Bacterial promoters, especially those of mesophilic bacteria, are in general preceded by a curved region. The marked thermosensitivity ...

journal_title：Biochemistry

authors： Prosseda G,Mazzola A,Di Martino ML,Tielker D,Micheli G,Colonna B

更新日期：2010-04-06 00:00:00

abstract：:A likely entry/exit path for nitrogenase substrates, products, and/or protons involves residues α277(Arg), α192(Ser), and α356(Gly), all of which are highly conserved among MoFe proteins from different organisms. The α192(Ser) and α277(Arg) residues form part of a hydrogen-bonded network that also involves α195(His), ...

journal_title：Biochemistry

authors： Fisher K,Hare ND,Newton WE

更新日期：2014-10-07 00:00:00

abstract：:The value of reducing the activity of ornithine decarboxylase (ODC), a key enzyme in the biosynthesis of polyamines, is well-appreciated. Polyamines are necessary components for cell growth, and manipulation of polyamine homeostasis may be an effective strategy for the treatment of a number of disorders, including neo...

journal_title：Biochemistry

authors： Ackermann JM,Kanugula S,Pegg AE

更新日期：2005-02-15 00:00:00

abstract：:Complex type N-glycosides of commercial bovine fetuin preparations from pooled fetal calf serum have been shown to contain comparable amounts of Gal4,4,4TRI (see structure A below) and Gal4,4,3TRI (structure B) as major asialo-structures. To investigate whether there is a clear genetic specificity for synthesis of the...

journal_title：Biochemistry

authors： Hayase T,Rice KG,Dziegielewska KM,Kuhlenschmidt M,Reilly T,Lee YC

更新日期：1992-05-26 00:00:00

abstract：:L-Aspartate oxidase (Laspo) catalyzes the conversion of L-Asp to iminoaspartate, the first step in the de novo biosynthesis of NAD(+). This bacterial pathway represents a potential drug target since it is absent in mammals. The Laspo R386L mutant was crystallized in the FAD-bound catalytically competent form and its t...

journal_title：Biochemistry

authors： Bossi RT,Negri A,Tedeschi G,Mattevi A

更新日期：2002-03-05 00:00:00

abstract：:Thermodynamic and structural evidence indicates that the DNA binding domains of lac repressor (lacI) exhibit significant conformational adaptability in operator binding, and that the marginally stable helix-turn-helix (HTH) recognition element is greatly stabilized by operator binding. Here we use circular dichroism a...

journal_title：Biochemistry

authors： Felitsky DJ,Record MT Jr

更新日期：2003-02-25 00:00:00

abstract：:The conversion of prion protein (PrP) to the pathogenic PrPSc conformation is central to prion disease. Previous studies revealed that PrP interacts with lipids and the interaction induces PrP conformational changes, yet it remains unclear whether in the absence of any denaturing treatment, PrP-lipid interaction is su...

journal_title：Biochemistry

authors： Wang F,Yang F,Hu Y,Wang X,Wang X,Jin C,Ma J

更新日期：2007-06-12 00:00:00

abstract：:Polyhydroxybutyrate (PHB) synthases catalyze the conversion of beta-hydroxybutyryl coenzyme A (HBCoA) to PHB. These enzymes require an active site cysteine nucleophile for covalent catalysis. A protein BLASTp search using the Class III Chromatium vinosum synthase sequence reveals high homology to prokaryotic lipases w...

journal_title：Biochemistry

authors： Jia Y,Kappock TJ,Frick T,Sinskey AJ,Stubbe J

更新日期：2000-04-11 00:00:00

abstract：:Zn2+ is tetrahedrally bonded to the 4 nonadjacent thiols of each regulatory chain (Mr 17,000) near r-c contacts between catalytic (c) and regulatory chains (r) in aspartate transcarbamoylase (ATCase; c6r6). This paper reports on Zn2+ interactions with r dimer in the absence of stabilizing r-c contacts. After r2 and c3...

journal_title：Biochemistry

authors： Jefferson JR,Hunt JB,Ginsburg A

更新日期：1990-07-17 00:00:00

abstract：:Prothrombin is the precursor of thrombin, a central enzyme in coagulation. Autoantibodies to prothrombin are associated with thromboembolism, but the mechanisms by which the antibodies modulate the coagulation processes are not understood. We screened a panel of 34 monoclonal antibody light chains isolated from patien...

journal_title：Biochemistry

authors： Thiagarajan P,Dannenbring R,Matsuura K,Tramontano A,Gololobov G,Paul S

更新日期：2000-05-30 00:00:00

abstract：:The fluorescence spectrum of C1 inhibitor (C1-Inh) in aqueous buffer has a maximum at 324 nm which shifts to 358 nm in 6.0 M guanidinium chloride (GdmC1), indicating that fluorescent tryptophans are buried in the native protein. When titrated with GdmC1, the fluorescence intensity, polarization, and emission maximum o...

journal_title：Biochemistry

authors： Lennick M,Brew SA,Ingham KC

更新日期：1985-05-07 00:00:00

abstract：:Natriuretic peptide receptor A (NPRA) is a noncovalent homodimeric receptor, composed of an extracellular domain (ECD) with a ligand-binding site, a single transmembrane domain (TM), and an intracellular domain (ICD) exhibiting guanylyl cyclase activity. NPRA activation by atrial natriuretic peptide (ANP) leads to cGM...

journal_title：Biochemistry

authors： Parat M,Blanchet J,De Léan A

更新日期：2010-06-08 00:00:00

abstract：:Fluorescence resonance energy transfer (FRET) has been used to investigate the conformation of the single stranded region for a series of fluorescent DNA template-primers bound to the Klenow fragment (KF) of Escherichia coli DNA polymerase I. Fluorescent derivatives of template-primer DNA, modified with tetramethylrho...

journal_title：Biochemistry

authors： Furey WS,Joyce CM,Osborne MA,Klenerman D,Peliska JA,Balasubramanian S

更新日期：1998-03-03 00:00:00

abstract：:βγ-Crystallins are important constituents of the vertebrate eye lens, whereas in microbes, they are prevalent as Ca2+-binding proteins. In archaea, βγ-crystallins are conspicuously confined to two methanogens, viz., Methanosaeta and Methanosarcina. One of these, i.e., M-crystallin from Methanosarcina acetivorans, has ...

journal_title：Biochemistry

authors： Srivastava SS,Jamkhindikar AA,Raman R,Jobby MK,Chadalawada S,Sankaranarayanan R,Sharma Y

更新日期：2017-03-07 00:00:00

abstract：:An amplified Okayama-Berg plasmid cDNA library was constructed from total poly(A)+ RNA isolated from the Madin-Darby bovine kidney cell line MDBK. This library was screened with a partial murine calpactin I heavy chain (p36) cDNA clone, the identification of which was based on bovine p36 tryptic peptide sequences gene...

journal_title：Biochemistry

authors： Kristensen T,Saris CJ,Hunter T,Hicks LJ,Noonan DJ,Glenney JR Jr,Tack BF

更新日期：1986-08-12 00:00:00

abstract：:Pseudomonas aeruginosa PAO1 encodes two outer membrane receptors, PhuR (Pseudomonas heme uptake) and HasR (heme assimilation system). The HasR receptor acquires heme through interaction with a secreted hemophore, HasAp. The non-hemophore-dependent PhuR is encoded along with proteins required for heme translocation int...

journal_title：Biochemistry

authors： Smith AD,Modi AR,Sun S,Dawson JH,Wilks A

更新日期：2015-04-28 00:00:00

abstract：:Taq and T7 DNA polymerases have become basic molecular biology "tools" for DNA sequence analysis. However, Taq, unlike T7 DNA polymerase, is strongly biased against the incorporation of 2',3'-dideoxynucleotide triphosphates (ddNTPs) indicating very different substrate selectivities. Equilibrium binding and rate consta...

journal_title：Biochemistry

authors： Brandis JW,Edwards SG,Johnson KA

更新日期：1996-02-20 00:00:00

abstract：:Molecules that bind DNA via threading intercalation show high binding affinity as well as slow dissociation kinetics, properties ideal for the development of anticancer drugs. To this end, it is critical to identify the specific molecular characteristics of threading intercalators that result in optimal DNA interactio...

journal_title：Biochemistry

authors： Clark AG,Naufer MN,Westerlund F,Lincoln P,Rouzina I,Paramanathan T,Williams MC

更新日期：2018-02-06 00:00:00