Dynamics of light-induced conformational changes of the phoborhodopsin/transducer complex formed in the n-dodecyl beta-D-maltoside micelle.

abstract：:To understand the relationship between the chemical structure of polar molecules and their membrane location, the behavior of dansyl (dimethylaminonaphthalenesulfonyl) and related polar fluorescent probes was examined. The depth of these probes in lipid bilayers was determined by parallax analysis of fluorescence quen...

journal_title：Biochemistry

authors： Asuncion-Punzalan E,Kachel K,London E

更新日期：1998-03-31 00:00:00

abstract：:In bacteria, two-component systems act as signaling systems to respond to environmental stimuli. Two-component systems generally consist of a sensor histidine kinase and a response regulator, which work together through histidyl-aspartyl phosphorelay to result in gene regulation. One of the two-component systems in Es...

journal_title：Biochemistry

authors： Affandi T,Issaian AV,McEvoy MM

更新日期：2016-09-20 00:00:00

abstract：:DNA ring closure experiments on short restriction fragments ( approximately 160 bp) bound by the TATA box binding protein (TBP) have demonstrated the formation of negative topoisomers, consistent with crystallographically observed TBP-induced DNA untwisting but in contrast to most previous results on topological effec...

journal_title：Biochemistry

authors： Kahn JD

更新日期：2000-04-04 00:00:00

abstract：:Horseradish peroxidase C binds a wide variety of small H-donor compounds such as benzohydroxamic acid (BHA) and 2-naphthohydroxamic acid (NHA). In this work, we use the Mg(II)-mesoporphyrin prosthetic group derivative as a spectroscopic probe of the active site and of the interaction with the substrates. We report on ...

journal_title：Biochemistry

authors： Laberge M,Osvath S,Fidy J

更新日期：2001-08-07 00:00:00

abstract：:Human heart short chain L-3-hydroxyacyl-CoA dehydrogenase (SCHAD) catalyzes the oxidation of the hydroxyl group of L-3-hydroxyacyl-CoA to a keto group, concomitant with the reduction of NAD+ to NADH, as part of the beta-oxidation pathway. The homodimeric enzyme has been overexpressed in Escherichia coli, purified to h...

journal_title：Biochemistry

authors： Barycki JJ,O'Brien LK,Bratt JM,Zhang R,Sanishvili R,Strauss AW,Banaszak LJ

更新日期：1999-05-04 00:00:00

abstract：:We studied hyperosmolarity-induced changes in cell volume and cytoplasmic pH in PS120 cells expressing Na(+)/H(+) exchanger (NHE) isoforms and their mutants. Change in cell volume was estimated by measuring change in cell height by means of confocal microscopy. Regulatory volume increase (RVI) and cytoplasmic alkalini...

journal_title：Biochemistry

authors： Su X,Pang T,Wakabayashi S,Shigekawa M

更新日期：2003-02-04 00:00:00

abstract：:The folding of the peptide chain of the bovine heart oxoglutarate carrier in the inner mitochondrial membrane and in the membrane of reconstituted proteoliposomes has been investigated by enzymatic and immunochemical approaches using proteinase K and polyclonal site-directed antibodies, respectively. Two peptides corr...

journal_title：Biochemistry

authors： Bisaccia F,Capobianco L,Brandolin G,Palmieri F

更新日期：1994-03-29 00:00:00

abstract：:Structural features of a 19-nucleotide RNA hairpin loop (5'-GGCGUACGUUUCGUACGCC-3'), a loop motif which occurs in eukaryotic 18S rRNA, have been derived using multidimensional heteronuclear NMR spectroscopy in combination with local conformational analysis and torsion angle distance geometry followed by restrained ene...

journal_title：Biochemistry

authors： Sich C,Ohlenschläger O,Ramachandran R,Görlach M,Brown LR

更新日期：1997-11-18 00:00:00

abstract：:The effect of normo (NTG)- and hypertriglyceridemic (HTG)-VLDL on cultured human umbilical vein endothelial cell (HUVEC) surface-localized fibrinolysis was examined following pre-incubation with NTG-, HTG-VLDL, LDL (1-20 micrograms/mL) or buffer (control). Ligand binding assays, using 125I-labeled tcu-PA, t-PA, or Glu...

journal_title：Biochemistry

authors： Li XN,Koons JC,Benza RL,Parks JM,Varma VK,Bradley WA,Gianturco SH,Taylor KB,Grammer JR,Tabengwa EM,Booyse FM

更新日期：1996-05-14 00:00:00

abstract：:C-type natriuretic peptide (CNP) binds the guanylyl cyclase-linked natriuretic peptide receptor B (NPR-B) and stimulates marked elevations of the intracellular signaling molecule, cGMP. Here, the essential role of phosphorylation in the hormonal activation and deactivation of this receptor is described. Exposure of NI...

journal_title：Biochemistry

authors： Potter LR

更新日期：1998-02-24 00:00:00

abstract：:The enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) catalyzes the reaction between shikimate 3-phosphate and phosphoenolpyruvate to form 5-enolpyruvylshikimate 3-phosphate, an intermediate in the shikimate pathway, which leads to the biosynthesis of aromatic amino acids. EPSPS exists in an open conformation...

journal_title：Biochemistry

authors： Marques MR,Vaso A,Neto JR,Fossey MA,Oliveira JS,Basso LA,dos Santos DS,de Azevedo Junior WF,Palma MS

更新日期：2008-07-15 00:00:00

abstract：:We have performed microsecond (μs) molecular dynamics simulation (MDS) to identify structural mechanisms for sarcolipin (SLN) uncoupling of Ca2+ transport from ATP hydrolysis for the sarcoplasmic reticulum Ca2+-ATPase (SERCA). SLN regulates muscle metabolism and energy expenditure to provide resistance against diet-in...

journal_title：Biochemistry

authors： Autry JM,Thomas DD,Espinoza-Fonseca LM

更新日期：2016-11-08 00:00:00

abstract：:N1-(5'-Phosphoribosyl)adenosine-5'-monophosphate cyclohydrolase (HisI, PR-AMP cyclohydrolase) is a central enzyme in histidine biosynthesis catalyzing the hydrolysis of the N1-C6 bond of the purine substrate, a reaction unique to this pathway. A source of the recombinant monofunctional Methanococcus vannielii PR-AMP c...

journal_title：Biochemistry

authors： D'Ordine RL,Klem TJ,Davisson VJ

更新日期：1999-02-02 00:00:00

abstract：:The inducible isoform of nitric oxide synthase (iNOS) and three zinc tetrathiolate mutants (C104A, C109A, and C104A/C109A) were expressed in Escherichia coli and purified. The mutants were found by ICP-AES and the zinc-specific PAR colorimetric assay to be zinc free, whereas the wild-type iNOS zinc content was 0.38 +/...

journal_title：Biochemistry

authors： Mitchell DA,Erwin PA,Michel T,Marletta MA

更新日期：2005-03-29 00:00:00

abstract：:The combination of UV/visible/near-IR variable-temperature magnetic circular dichroism (VTMCD) and EPR spectroscopies has been used to investigate the spin states and axial ligation of the heme group in oxidized, reduced, and CO-bound reduced forms of the Rhodospirillum rubrum CO oxidation transcriptional activator pr...

journal_title：Biochemistry

authors： Dhawan IK,Shelver D,Thorsteinsson MV,Roberts GP,Johnson MK

更新日期：1999-09-28 00:00:00

abstract：:The binding of bleomycin to DNA in the presence and absence of ferric iron was measured by fluorescence spectroscopy. In millimolar concentrations of tris(hydroxymethyl)aminomethane, pH 7.5, approximately 80% of the bleomycin binds to DNA. Ferric iron seems to have no significant effect on the binding of DNA to bleomy...

journal_title：Biochemistry

authors： Caspary WJ,Lanzo DA,Niziak C

更新日期：1982-01-19 00:00:00

abstract：:A full-length cDNA for dopamine beta-monooxygenase (D beta M) from bovine adrenal glands has been cloned and sequenced. The soluble and membrane-derived forms of D beta M have also been sequenced from their N-termini. While the observed sequences for the soluble protein correspond to those previously reported [Joh, T....

journal_title：Biochemistry

authors： Taljanidisz J,Stewart L,Smith AJ,Klinman JP

更新日期：1989-12-26 00:00:00

abstract：:The structural underpinnings of enzyme substrate specificity are investigated in a pair of copper amine oxidases (CAOs) from Hansenula polymorpha (HPAO-1 and HPAO-2). The X-ray crystal structure (to 2.0 A resolution) and steady state kinetic data of the second copper amine oxidase (HPAO-2) are presented for comparison...

journal_title：Biochemistry

authors： Chang CM,Klema VJ,Johnson BJ,Mure M,Klinman JP,Wilmot CM

更新日期：2010-03-23 00:00:00

abstract：:B₁₂-dependent enzymes employ radical species with exceptional prowess to catalyze some of the most chemically challenging, thermodynamically unfavorable reactions. However, dealing with highly reactive intermediates is an extremely demanding task, requiring sophisticated control strategies to prevent unwanted side rea...

journal_title：Biochemistry

authors： Brunk E,Kellett WF,Richards NG,Rothlisberger U

更新日期：2014-06-17 00:00:00

abstract：:A T203Y substitution in green fluorescent protein causes a red shift in emission to yield a class of mutants known as yellow fluorescent protein (YFP). Many of these YFP mutants bind halides with affinities in the millimolar range, which often results in the chromophore pK values being shifted into the physiological r...

journal_title：Biochemistry

authors： Seward HE,Basran J,Denton R,Pfuhl M,Muskett FW,Bagshaw CR

更新日期：2013-04-09 00:00:00

abstract：:Despite many studies suggesting the role of enolpyruvate as a bound intermediate in the pyruvate kinase reaction, direct evidence for it has been lacking. By use of a combination of chemical trapping and isolation of a derivative, significant amounts of enzyme-bound enolpyruvate have now been demonstrated. The method ...

journal_title：Biochemistry

authors： Seeholzer SH,Jaworowski A,Rose IA

更新日期：1991-01-22 00:00:00

abstract：:The bovine adrenodoxin gene gives rise to two species of mRNA differing only at their 5'-ends. The synthesis of these two types of mRNA in bovine adrenal cortical cells is regulated transcriptionally in part by ACTH via the action of cAMP. Examination of the 5'-end of the adrenodoxin gene revealed that each mRNA conta...

journal_title：Biochemistry

authors： Chen JY,Waterman MR

更新日期：1992-03-03 00:00:00

abstract：:The S-protein/vitronectin gene was isolated from a human genomic DNA library, and its sequence of about 5.3 kilobases including the adjacent 5' and 3' flanking regions was established. Alignment of the genomic DNA nucleotide sequence and the cDNA sequence indicated that the gene consisted of eight exons and seven intr...

journal_title：Biochemistry

authors： Jenne D,Stanley KK

更新日期：1987-10-20 00:00:00

abstract：:Bovine brain adenylate cyclase was solubilized with 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS), sodium cholate, sodium deoxycholate, or these detergents plus (NH4)2SO4. The specific activity of the extract obtained with 13 mM CHAPS alone was several times those of the other detergent extracts wi...

journal_title：Biochemistry

authors： Bitonti AJ,Moss J,Hjelmeland L,Vaughan M

更新日期：1982-07-20 00:00:00

abstract：:One- and two-dimensional NMR spectroscopy and energy minimization calculations were used to investigate the conformation of a 2-aminofluorene- (AF-) modified model human c-H-ras1 protooncogene codon 61 deoxyoligonucleotide duplex, d(C1-A2-C3-C4-A5- [AF-G6]-G7-A8-A9-C10).-d(G11-T12-T13-C14-C15-T16 -G17-G18-T19-G20), in...

journal_title：Biochemistry

authors： Eckel LM,Krugh TR

更新日期：1994-11-22 00:00:00

abstract：:The interaction of insulin with its receptor is complex. Kinetic and equilibrium binding studies suggest coexistence of high- and low-affinity binding sites or negative cooperativity. These phenomena and high-affinity interactions are dependent on the dimeric structure of the receptor. Structure-function studies of in...

journal_title：Biochemistry

authors： Whittaker L,Hao C,Fu W,Whittaker J

更新日期：2008-12-02 00:00:00

abstract：:To probe the motional and conformational properties of the choline head group of 1,2-dipalmitoyl-sn-glycero-3-thiophosphocholine (DPPsC), the Rp, Sp, and Rp + Sp isomers of [alpha-D2]DPPsC, [beta-D2]DPPsC, and [delta-D9]DPPsC in the subgel, gel, and liquid crystalline phases were investigated with deuterium NMR, and t...

journal_title：Biochemistry

authors： Loffredo WM,Jiang RT,Tsai MD

更新日期：1990-12-11 00:00:00

abstract：:The cytochrome aa3-type terminal quinol oxidase of Bacillus subtilis catalyzes the four-electron reduction of dioxygen to water. It resembles the aa3-type cytochrome-c oxidase in using heme A as its active-site chromophores but lacks the CuA center and the cytochrome-c oxidizing activity of the mitochondrial enzyme. W...

journal_title：Biochemistry

authors： Lauraeus M,Wikström M,Varotsis C,Tecklenburg MM,Babcock GT

更新日期：1992-10-20 00:00:00

abstract：:Homoserine kinase, an enzyme in the aspartate pathway of amino acid biosynthesis in Escherichia coli, catalyzes the conversion of L-homoserine to L-homoserine phosphate. This enzyme has been found to have broad substrate specificity, including the phosphorylation of L-homoserine analogs where the carboxyl functional g...

journal_title：Biochemistry

authors： Huo X,Viola RE

更新日期：1996-12-17 00:00:00

abstract：:FtmPT1 is a fungal indole prenyltransferase that catalyzes the reaction of tryptophan derivatives with dimethylallyl pyrophosphate to form various biologically active compounds. Herein, we describe detailed studies of FtmPT1 catalysis involving dimethylallyl pyrophosphate and Brevianamide F following the native pathwa...

journal_title：Biochemistry

authors： Pan LL,Yang Y,Merz KM Jr

更新日期：2014-09-30 00:00:00