Transcriptional regulation of the nuclear gene encoding the alpha-subunit of the mammalian mitochondrial F1F0 ATP synthase complex: role for the orphan nuclear receptor, COUP-TFII/ARP-1.

Abstract:

:Our laboratory has been studying the transcriptional regulation of the nuclear gene (ATPA) that encodes the alpha-subunit of the mammalian mitochondrial F1F0 ATP synthase complex. We have previously determined that the regulatory factor, upstream stimulatory factor 2 (USF2), can stimulate transcription of the ATPA gene through the cis-acting regulatory element 1 in the upstream promoter of this gene. In this study, we used the yeast one-hybrid screening method to identify another factor, COUP-TFII/ARP-1, which also binds to the ATPA cis-acting regulatory element 1. Binding of the orphan nuclear receptor, COUP-TFII/ARP-1, to the ATPA regulatory element 1 was confirmed using electrophoretic mobility shift experiments, and COUP-TFII/ARP-1-containing complexes were detected in HeLa cell nuclear extracts. A mutational analysis indicated that the binding site for COUP-TFII/ARP-1 in the ATPA regulatory element 1 is an imperfect direct repeat of a nuclear receptor response element (A/GGGTCA) with a spacer of three nucleotides. Functional assays in HeLa cells showed that COUP-TFII/ARP-1 represses the ATPA promoter activity in a dose- and sequence-dependent manner. Furthermore, cotransfection assays demonstrated that COUP-TFII/ARP-1 inhibits the USF2-mediated activation of the wild-type ATPA gene promoter but not a mutant promoter that is defective in COUP-TFII/ARP-1-binding. Overexpression of USF2 reversed the COUP-TFII/ARP-1-mediated repression of the ATPA promoter. Mobility shift assays revealed that COUP-TFII/ARP-1 and USF2 compete for binding to the ATPA regulatory element 1. Thus, the ATPA gene is regulated by a multifunctional binding site through which the transcription factors, COUP-TFII/ARP-1 and USF2, bind and exert their antagonistic effects.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Jordan EM,Worley T,Breen GA

doi

10.1021/bi0268347

subject

Has Abstract

pub_date

2003-03-11 00:00:00

pages

2656-63

issue

9

eissn

0006-2960

issn

1520-4995

journal_volume

42

pub_type

杂志文章
  • Characterization of interactions between a two-component response regulator, Spo0F, and its phosphatase, RapB.

    abstract::The phosphorelay signal transduction pathway controls sporulation initiation in Bacillus subtilis. Transfer of a phosphoryl group from multiple kinases (KinA and KinB) through a single domain response regulator homologue (Spo0F), a phosphotransferase (Spo0B), and ultimately to a transcriptional regulator, (Spo0A) acti...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi981340o

    authors: Tzeng YL,Feher VA,Cavanagh J,Perego M,Hoch JA

    更新日期:1998-11-24 00:00:00

  • Structural determinants for plant annexin-membrane interactions.

    abstract::The interactions of two plant annexins, annexin 24(Ca32) from Capsicum annuum and annexin Gh1 from Gossypium hirsutum, with phospholipid membranes have been characterized using liposome-based assays and adsorption to monolayers. These two plant annexins show a preference for phosphatidylserine-containing membranes and...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi0516226

    authors: Dabitz N,Hu NJ,Yusof AM,Tranter N,Winter A,Daley M,Zschörnig O,Brisson A,Hofmann A

    更新日期:2005-12-13 00:00:00

  • Linkage between operator binding and dimer to octamer self-assembly of bacteriophage lambda cI repressor.

    abstract::Cooperative binding of the bacteriophage lambda cI repressor dimer to specific sites of the phage operators OR and OL controls the developmental state of the phage. Cooperativity has long been thought to be mediated by self-assembly of repressor dimers to form tetramers which can bind simultaneously to adjacent operat...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi971368k

    authors: Rusinova E,Ross JB,Laue TM,Sowers LC,Senear DF

    更新日期:1997-10-21 00:00:00

  • Chimeric human calcitonin and glucagon receptors reveal two dissociable calcitonin interaction sites.

    abstract::Two chimeric receptors were constructed by transposing the coding regions for the putative N-terminal domains of the human calcitonin (hCTR) and glucagon (hGGR) receptors. These receptors were stably expressed as glycosylated proteins with molecular masses of 80 kDa for the calcitonin receptor N-terminus chimera (NtCT...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00003a040

    authors: Stroop SD,Kuestner RE,Serwold TF,Chen L,Moore EE

    更新日期:1995-01-24 00:00:00

  • Identification of determinants for inhibitor binding within the RNA active site of human telomerase using PNA scanning.

    abstract::Telomerase is a ribonucleoprotein that participates in the maintenance of telomere length. Its activity is up-regulated in many tumor types, suggesting that it may be a novel target for chemotherapy. The RNA component of telomerase contains an active site that plays at least two roles&sbd;binding telomere ends and tem...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi970438k

    authors: Hamilton SE,Pitts AE,Katipally RR,Jia X,Rutter JP,Davies BA,Shay JW,Wright WE,Corey DR

    更新日期:1997-09-30 00:00:00

  • Binding of polyaminocarboxylate chelators to the active-site copper inhibits the GSNO-reductase activity but not the superoxide dismutase activity of Cu,Zn-superoxide dismutase.

    abstract::In addition to its superoxide dismutase (SOD) activity, Cu,Zn-superoxide dismutase (CuZnSOD) catalyzes the reductive decomposition of S-nitroso-L-glutathione (GSNO) in the presence of thiols such as L-glutathione (GSH). The GSNO-reductase activity but not the superoxide dismutase (SOD) activity of CuZnSOD is inhibited...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi061077o

    authors: Ye M,English AM

    更新日期:2006-10-24 00:00:00

  • An unusual pattern of CytR and CRP binding energetics at Escherichia coli cddP suggests a unique blend of class I and class II mediated activation.

    abstract::Two transcription factors, CRP and CytR, mediate positive and negative control of nine cistrons involved in nucleoside catabolism and recycling in Escherichia coli. The ability of multiple transcription factors to combine in different ways to confer differential gene regulation is of significant interest in both proka...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi901583n

    authors: Holt AK,Senear DF

    更新日期:2010-01-26 00:00:00

  • Structural and thermodynamic consequences of burying a charged residue within the hydrophobic core of T4 lysozyme.

    abstract::To determine the energetic and structural consequences of placing a charged group within the core of a protein, two "buried charge" mutants, Met 102----Lys (M102K) and Leu 133----Asp (L133D) were constructed in phage T4 lysozyme. Both proteins fold at neutral pH, although they are substantially less stable than wild t...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00113a006

    authors: Dao-pin S,Anderson DE,Baase WA,Dahlquist FW,Matthews BW

    更新日期:1991-12-10 00:00:00

  • Influence of base stacking and hydrogen bonding on the fluorescence of 2-aminopurine and pyrrolocytosine in nucleic acids.

    abstract::Fluorescent nucleobase analogues are used extensively to probe the structure and dynamics of nucleic acids. The fluorescence of the adenine analogue 2-aminopurine and the cytosine analogue pyrrolocytosine is significantly quenched when the bases are located in regions of double-stranded nucleic acids. To allow more de...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi060479t

    authors: Hardman SJ,Thompson KC

    更新日期:2006-08-01 00:00:00

  • Pinpoint Chemical Modification of the Quinone-Access Channel of Mitochondrial Complex I via a Two-Step Conjugation Reaction.

    abstract::We previously showed that a bulky ring-strained cycloalkyne possessing a rhodamine fluorophore directly reacts (via strain-promoted click chemistry) with the azido group incorporated (via ligand-directed tosyl chemistry) into Asp160 in the 49 kDa subunit of complex I in bovine heart submitochondrial particles [Masuya,...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/acs.biochem.7b00612

    authors: Masuya T,Murai M,Ito T,Aburaya S,Aoki W,Miyoshi H

    更新日期:2017-08-15 00:00:00

  • Transient-state kinetics of apurinic/apyrimidinic (AP) endonuclease 1 acting on an authentic AP site and commonly used substrate analogs: the effect of diverse metal ions and base mismatches.

    abstract::Apurinic/apyrimidinic endonuclease 1 (APE1) is an Mg²⁺-dependent enzyme responsible for incising the DNA backbone 5' to an apurinic/apyrimidinic (AP) site. Here, we use rapid quench flow (RQF) techniques to provide a comprehensive kinetic analysis of the strand-incision activity (k(chemistry)) of APE1 acting on an aut...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi401218r

    authors: Schermerhorn KM,Delaney S

    更新日期:2013-10-29 00:00:00

  • Nucleotide- and substrate-induced conformational transitions in the CBS domain-containing pyrophosphatase of Moorella thermoacetica.

    abstract::In contrast to all other known pyrophosphatases, Moorella thermoacetica pyrophosphatase (mtCBS-PPase) contains nucleotide-binding CBS domains and is thus strongly regulated by adenine nucleotides. Stopped-flow measurements using a fluorescent AMP analogue, 2'(3')-O-(N-methylanthranoyl)-AMP (Mant-AMP), reveal that nucl...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi9019737

    authors: Jämsen J,Baykov AA,Lahti R

    更新日期:2010-02-09 00:00:00

  • Spin equilibrium and quaternary structure change in hemoglobin A. Experiments on a quantitative probe of the stereochemical mechanism of hemoglobin cooperativity.

    abstract::The molecular mechanism of hemoglobin cooperativity was studied kinetically by flash photolysis on mixed-state hemoglobins which consist of three ferrous carboxy subunits and one hybrid ferric subunit including fluoromet, azidomet, cyanatomet, and thiocyanatomet. The effects of conformational transitions on the hybrid...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00593a012

    authors: Cho KC,Hopfield JJ

    更新日期:1979-12-25 00:00:00

  • G-1:C73 recognition by an arginine cluster in the active site of Escherichia coli histidyl-tRNA synthetase.

    abstract::Aminoacylation of a transfer RNA (tRNA) by its cognate aminoacyl-tRNA synthetase relies upon the recognition of specific nucleotides as well as conformational features within the tRNA by the synthetase. In Escherichia coli, the aminoacylation of tRNA(His) by histidyl-tRNA synthetase (HisRS) is highly dependent upon th...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi035708f

    authors: Connolly SA,Rosen AE,Musier-Forsyth K,Francklyn CS

    更新日期:2004-02-03 00:00:00

  • Biochemical and Functional Evaluation of the Intramolecular Disulfide Bonds in the Zinc-Chelating Antimicrobial Protein Human S100A7 (Psoriasin).

    abstract::Human S100A7 (psoriasin) is a metal-chelating protein expressed by epithelial cells. It is a 22-kDa homodimer with two EF-hand domains per subunit and two transition-metal-binding His3Asp sites at the dimer interface. Each subunit contains two cysteine residues that can exist as free thiols (S100A7red) or as an intram...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/acs.biochem.7b00781

    authors: Cunden LS,Brophy MB,Rodriguez GE,Flaxman HA,Nolan EM

    更新日期:2017-10-31 00:00:00

  • Evidence from base-pair kinetics for two types of adenine tract structures in solution: their relation to DNA curvature.

    abstract::We have measured the base-pair lifetimes in oligodeoxynucleotides containing tracts of A.T base pairs using imino proton magnetic resonance. When the tract contains more than four consecutive A.T base pairs, possibly including a 5'-AT step but not a 5'-TA step, anomalously long lifetimes are observed. For example, the...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00425a004

    authors: Leroy JL,Charretier E,Kochoyan M,Guéron M

    更新日期:1988-12-13 00:00:00

  • Heme-CO religation in photolyzed hemoglobin: a time-resolved Raman study of the Fe-CO stretching mode.

    abstract::Time-resolved resonance Raman spectroscopy has been employed to monitor geminate heme-CO rebinding in photolyzed HbCO. The excitation frequency was tuned to enhance the scattering from rebound heme sites 20-500 ns subsequent to CO photolysis. The behavior of vFe-C during ligand rebinding has important ramifications co...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00056a017

    authors: Schneebeck MC,Vigil LE,Friedman JM,Chavez MD,Ondrias MR

    更新日期:1993-02-09 00:00:00

  • Proteolysis of the cytochrome d complex with trypsin and chymotrypsin localizes a quinol oxidase domain.

    abstract::The cytochrome d complex is a two-subunit, membrane-bound terminal oxidase in the aerobic respiratory chain of Escherichia coli. The enzyme catalyzes the two-electron oxidation of ubiquinol and the four-electron reduction of oxygen to water. Previous work demonstrated that the site for ubiquinol oxidation was selectiv...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00228a007

    authors: Dueweke TJ,Gennis RB

    更新日期:1991-04-09 00:00:00

  • Laser temperature jump study of solvent effects of poly(adenylic acid) stacking.

    abstract::The dynamics of the single-stranded, helix--coil transition of poly(adenylic acid) have been investigated by using the Raman laser temperature jump technique. The driving forces of this conformational transition have been probed by varying the cosolvent in mixed aqueous solutions. The rate of helix formation correlate...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00549a025

    authors: Dewey TG,Turner DH

    更新日期:1980-04-15 00:00:00

  • Molecular structure of Escherichia coli PurT-encoded glycinamide ribonucleotide transformylase.

    abstract::In Escherichia coli, the PurT-encoded glycinamide ribonucleotide transformylase, or PurT transformylase, catalyzes an alternative formylation of glycinamide ribonucleotide (GAR) in the de novo pathway for purine biosynthesis. On the basis of amino acid sequence analyses, it is known that the PurT transformylase belong...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi000926j

    authors: Thoden JB,Firestine S,Nixon A,Benkovic SJ,Holden HM

    更新日期:2000-08-01 00:00:00

  • Translocase-bound SecA is largely shielded from the phospholipid acyl chains.

    abstract::Protein translocation in Escherichia coli is mediated by the SecA ATPase bound to the SecYEG membrane protein complex. SecA translocation ATPase activity as well as protein translocation is dependent on the presence of negatively charged lipids. By using a phospholipid with an acyl chain linked photoactivatable group,...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi9809021

    authors: van Voorst F,van der Does C,Brunner J,Driessen AJ,de Kruijff B

    更新日期:1998-09-01 00:00:00

  • Nuclear magnetic resonance and neutron diffraction studies of the complex of ribonuclease A with uridine vanadate, a transition-state analogue.

    abstract::The complex of ribonuclease A (RNase A) with uridine vanadate (U-V), a transition-state analogue, has been studied with 51V and proton NMR spectroscopy in solution and by neutron diffraction in the crystalline state. Upon the addition of aliquots of U-V at pH 6.6, the C epsilon-H resonances of the two active-site hist...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00329a038

    authors: Borah B,Chen CW,Egan W,Miller M,Wlodawer A,Cohen JS

    更新日期:1985-04-09 00:00:00

  • Activation of Bordetella pertussis adenylate cyclase by the carboxyl-terminal tryptic fragment of calmodulin.

    abstract::Highly purified tryptic fragments of calmodulin were tested for their ability to stimulate adenylate cyclase activity of Bordetella pertussis spheroplast membranes and were compared to their activities on brain Ca2+/calmodulin-dependent cyclic nucleotide phosphodiesterase. The C-terminal fragment, consisting of residu...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00372a025

    authors: Wolff J,Newton DL,Klee CB

    更新日期:1986-12-02 00:00:00

  • An allosteric intramolecular PDZ-PDZ interaction modulates PTP-BL PDZ2 binding specificity.

    abstract::PDZ (acronym of the synapse-associated protein PSD-95/SAP90, the septate junction protein Discs-large, and the tight junction protein ZO-1) domains are abundant small globular protein interaction domains that mainly recognize the carboxyl termini of their target proteins. Detailed knowledge on PDZ domain binding speci...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi700954e

    authors: van den Berk LC,Landi E,Walma T,Vuister GW,Dente L,Hendriks WJ

    更新日期:2007-11-27 00:00:00

  • Protoheme-carbon monoxide geminate kinetics.

    abstract::Recombination kinetics of CO to protoheme after laser photolysis have been measured vs. temperature and viscosity. A 25-ns laser pulse was focused on the sample to produce an excitation rate of 10(9)/s per heme. This temporarily populates the heme-CO state of dissociated pairs which either separate or recombine on a p...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00358a008

    authors: Marden MC,Hazard ES 3rd,Gibson QH

    更新日期:1986-05-20 00:00:00

  • Structural studies of acetylated and control inner core histones.

    abstract::The role of acetylation on the conformation and association state of the inner core histone octamer isolated from HeLa cells was examined. Preparation of suitable quantities of pure acetylated and control inner core histones from HeLa cells required the development of a new preparative procedure. The results from size...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi00384a041

    authors: Prevelige PE Jr,Fasman GD

    更新日期:1987-05-19 00:00:00

  • Importance of central alpha-helices of human apolipoprotein A-I in the maturation of high-density lipoproteins.

    abstract::We have studied the role of amphipathic alpha-helices in the ability of apoA-I to promote cholesterol efflux from human skin fibroblasts and activate lecithin:cholesterol acyltransferase (LCAT). Three apoA-I mutants were designed, each by deletion of a pair of predicted adjacent central alpha-helices [Delta(100-143), ...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi981205b

    authors: Frank PG,N'Guyen D,Franklin V,Neville T,Desforges M,Rassart E,Sparks DL,Marcel YL

    更新日期:1998-09-29 00:00:00

  • Metal-ion-dependent catalysis and specificity of CCA-adding enzymes: a comparison of two classes.

    abstract::The CCA-adding enzymes [ATP(CTP):tRNA nucleotidyl transferases] catalyze synthesis of the conserved and essential CCA sequence to the tRNA 3' end. These enzymes are divided into two classes of distinct structures that differ in the overall orientation of the head to tail domains. However, the catalytic core of the two...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi0509402

    authors: Hou YM,Gu SQ,Zhou H,Ingerman L

    更新日期:2005-09-27 00:00:00

  • One-Enzyme Reverse Transcription qPCR Using Taq DNA Polymerase.

    abstract::Taq DNA polymerase, one of the first thermostable DNA polymerases to be discovered, has been typecast as a DNA-dependent DNA polymerase commonly employed for PCR. However, Taq polymerase belongs to the same DNA polymerase superfamily as the Molony murine leukemia virus reverse transcriptase and has in the past been sh...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/acs.biochem.0c00778

    authors: Bhadra S,Maranhao AC,Paik I,Ellington AD

    更新日期:2020-12-15 00:00:00

  • The formylglycinamide ribonucleotide amidotransferase complex from Bacillus subtilis: metabolite-mediated complex formation.

    abstract::Formylglycinamide ribonucleotide amidotransferase (FGAR-AT) catalyzes the ATP- and glutamine-dependent formation of formylglycinamidine ribonucleotide, ADP, P(i), and glutamate in the fourth step of de novo purine biosynthesis. Like all amidotransferases (ATs), FGAR-AT is proposed to channel ammonia between a glutamin...

    journal_title:Biochemistry

    pub_type: 杂志文章

    doi:10.1021/bi049127h

    authors: Hoskins AA,Anand R,Ealick SE,Stubbe J

    更新日期:2004-08-17 00:00:00