Abstract:
:GTPase-activating proteins (GAPs) enhance the intrinsic GTPase activity of small G proteins, such as Ras and Rho, by contributing a catalytic arginine to the active site. An intramolecular arginine plays a similar role in heterotrimeric G proteins. Aluminum fluoride activates the GDP form of heterotrimeric G proteins, and enhances binding of the GDP form of small G proteins to their GAPs. The resultant complexes have been interpreted as analogues of the transition state of the hydrolytic reaction. Here, equilibrium binding has been measured using scintillation proximity assays to provide quantitative information on the fluoride-mediated interaction of Ras and Rho proteins with their respective GAPs, neurofibromin (NF1) and RhoGAP. High-affinity fluoride-mediated complex formation between Rho.GDP and RhoGAP occurred in the absence of aluminum; however, under these conditions, magnesium was required. Additionally, the novel observation was made of magnesium-dependent, fluoride-mediated binding of Ras.GDP to NF1 in the absence of aluminum. Aluminum was required for complex formation when the concentration of magnesium was low. Thus, either aluminum fluoride or magnesium fluoride can mediate the high-affinity binding of Rho. GDP or Ras.GDP to GAPs. It has been reported that magnesium fluoride can activate heterotrimeric G proteins. Thus, magnesium-dependent fluoride effects might be a general phenomenon with G proteins. Moreover, these data suggest that some protein.nucleotide complexes previously reported to contain aluminum fluoride may in fact contain magnesium fluoride.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Graham DL,Eccleston JF,Chung CW,Lowe PNdoi
10.1021/bi991358esubject
Has Abstractpub_date
1999-11-09 00:00:00pages
14981-7issue
45eissn
0006-2960issn
1520-4995pii
bi991358ejournal_volume
38pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Studies were undertaken to assess the postulated involvement of subunit III in the proton-linked functions of cytochrome c oxidase. The effect of pH on the steady-state kinetic [corrected] parameters of subunit III containing and subunit III depleted cytochrome oxidase was determined by using beef heart and rat liver ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00417a016
更新日期:1988-08-23 00:00:00
abstract::The beta- and delta-subunits of the nicotinic acetylcholine receptor from Torpedo californica were covalently photolabeled at the synaptic surface with the ATP photoaffinity analogue [alpha-32P]-8-azido-ATP. The specificity of labeling for nucleotide binding sites was demonstrated by the saturation of labeling with in...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00080a002
更新日期:1993-07-27 00:00:00
abstract::Solvent proton nuclear magnetic dispersion studies at 25, 100, and 300 MHz have been performed on protocatechuate 3,4-dioxygenase (PCD) and its complexes with 3-chloro-4-hydroxybenzoate and 3-fluoro-4-hydroxybenzoate. Longitudinal and transverse relaxation rates were measured for these compounds and for the apoenzyme....
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00312a024
更新日期:1984-08-14 00:00:00
abstract::When stoichiometric amounts of tropomyosin (TM) are bound to F-actin in the presence of 2 mM ATP, the MG2+-activated acto-heavy meromyosin (HMM) ATPase is inhibited by about 60% in 5 mM MgCl2-30 mM KCl. If the concentration of MgCl2 is reduced to 1 mM, the inhibition disappears because TM no longer binds to F-actin. I...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00683a025
更新日期:1975-06-17 00:00:00
abstract::Redox transitions in the Rhodobacter capsulatus cytochrome bc(1) complex were investigated by perfusion-induced attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy combined with synchronous visible spectroscopy, in both the wild type and a cytochrome c(1) point mutant, M183K, in which the mi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi049211x
更新日期:2004-07-27 00:00:00
abstract::The membrane-bound closed channel structure of helices 4 and 5 (Lys-406-Asp-446) of the colicin E1 channel domain was investigated by using a site-directed fluorescence labeling technique. A bimane probe was covalently attached to a cysteine residue in a series of single-cysteine mutant proteins to scan each helix in ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi801906v
更新日期:2009-02-17 00:00:00
abstract::The complete amino acid sequence of the major component myoglobin from Amazon River dolphin, Inia geoffrensis, was determined by specific cleavage of the protein to obtain large peptides which are readily degraded by the automatic sequencer. Three easily separable peptides were obtained by cleaving the protein with cy...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00695a018
更新日期:1975-12-02 00:00:00
abstract::Two sites of ligand interaction in acetylcholinesterase (AChE) were first demonstrated in ligand binding studies and later confirmed by crystallography, site-specific mutagenesis, and molecular modeling: an acylation site at the base of the active site gorge and a peripheral site at its mouth. We recently introduced a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9813577
更新日期:1999-01-05 00:00:00
abstract::Chemical deglycosylation of gonadotropins with hydrogen fluoride (HF) has facilitated the investigation of the structure-function relationship of the individual peptide and oligosaccharide moieties in the mechanism of hormone action. These studies have dealt almost exclusively with lutropin or human choriogonadotropin...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00361a030
更新日期:1986-07-01 00:00:00
abstract::Ornithine decarboxylase (ODC) catalyzes the first committed step in the biosynthesis of polyamines, and it has been identified as a drug target for the treatment of African sleeping sickness, caused by Trypanosoma brucei. ODC is a pyridoxal 5'-phosphate (PLP) dependent enzyme and an obligate homodimer. X-ray structura...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi001209s
更新日期:2000-09-19 00:00:00
abstract::Vitamin D receptor (VDR) is a member of the nuclear hormone receptor superfamily. When bound to a variety of vitamin D analogues, VDR manifests a wide diversity of physiological actions. The molecular mechanism by which different vitamin D analogues cause specific responses is not understood. The published crystallogr...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi201637p
更新日期:2011-12-27 00:00:00
abstract::Folylpolyglutamate synthetase was purified 30,000-150,000-fold from hog liver. Purification required the use of protease inhibitors, and the protein was purified to homogeneity in two forms. Both forms of the enzyme were monomers of Mr 62,000 and had similar specific activities. The specific activity of the homogeneou...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00376a024
更新日期:1987-01-27 00:00:00
abstract::We have measured the pH-dependent (1)H, (13)C, and (15)N NMR spectra of pyridoxal 5'-phosphate ((13)C(2)-PLP) mixed with equal amounts of either doubly (15)N-labeled diaminopropane, (15)N(α)-labeled l-lysine, or (15)N(ε)-labeled l-lysine as model systems for various intermediates of the transimination reaction in PLP-...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi101061m
更新日期:2010-12-28 00:00:00
abstract::The structure of the vanadate-trapped ADP complex of a truncated head of Dictyostelium myosin II consisting of residues Asp 2-Asn 762 has been determined by molecular replacement at 1.9 A resolution and refined to a crystallographic R-factor of 19.4%. The crystals belong to the orthorhombic space group C2221 where a =...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi952633+
更新日期:1996-04-30 00:00:00
abstract::The temporal evolution of the near-infrared stimulated emission band of the special pair excited state (P*) in the reaction center of Rhodobacter sphaeroides has been studied in intracytoplasmic membranes of the antenna-deficient RCO1 mutant at 10 K with a resolution of 30 fs. On the 100-fs time scale the emission ban...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00188a002
更新日期:1994-06-07 00:00:00
abstract::The water-oxidizing complex of photosystem II cycles through five oxidation states, denoted S(i)() (i = 0-4), during water oxidation to molecular oxygen, which appears at the (transient) S(4) state. The recent detection of bimodal EPR signals from the S(3) state [Matsukawa, T., Mino, H., Yoneda, D., Kawamori, A. (1999...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0159938
更新日期:2002-07-30 00:00:00
abstract::Nonsense mutations that result in premature stop codons in the HBB gene cause β-thalassemia. This disease is characterized by a reduced hemoglobin level due to the lack of β-globin. Compounds that induce translational readthrough across the thalassemia-causing premature stop codon will have therapeutic benefits. Curre...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.9b00761
更新日期:2020-01-14 00:00:00
abstract::A conserved, crystallographically defined bile acid binding site was originally identified in the membrane domain of mammalian and bacterial cytochrome c oxidase (CcO). Current studies show other amphipathic molecules including detergents, fatty acids, steroids, and porphyrins bind to this site and affect the already ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi3014505
更新日期:2013-02-26 00:00:00
abstract::Metabolomics is a rapidly growing field of research used in the identification and quantification of the small molecule metabolites within an organism, thereby providing insights into cell metabolism and bioenergetics as well as processes important in clinical medicine, such as disposition of pharmaceutical compounds....
journal_title:Biochemistry
pub_type: 杂志文章,评审
doi:10.1021/bi400060e
更新日期:2013-06-04 00:00:00
abstract::The side chains of histidine and aspartate residues form a hydrogen bond in the active sites of many enzymes. In serine proteases, the His...Asp hydrogen bond of the catalytic triad is known to contribute greatly to catalysis, perhaps via the formation of a low-barrier hydrogen bond. In bovine pancreatic ribonuclease ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi972766q
更新日期:1998-06-23 00:00:00
abstract::The specificity of subtilisin BPN' toward substrates with large hydrophobic P4 residues has been improved by single amino acid replacements at positions 104 and 107. Mutations were designed to (i) increase the size of the P4 binding pocket by replacing Ile107, which is at the bottom of the S4 pocket, by Val, Ala, and ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00056a001
更新日期:1993-02-09 00:00:00
abstract::UDP-GlcNAc 2-epimerase/ManNAc kinase (GNE) catalyzes the first two committed steps in sialic acid synthesis. In addition to the three previously described human GNE isoforms (hGNE1-hGNE3), our database and polymerase chain reaction analysis yielded five additional human isoforms (hGNE4-hGNE8). hGNE1 is the ubiquitousl...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi201050u
更新日期:2011-10-18 00:00:00
abstract::The continued rise of antibiotic-resistant infections coupled with the limited pipeline of new antimicrobials highlights the pressing need for the development of new antibacterial agents. One potential pathway for new agents is de novo purine biosynthesis as studies have shown that bacteria and lower eukaryotes synthe...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b00939
更新日期:2019-04-30 00:00:00
abstract::The relative roles of insulin and glucose in the regulation of hepatic glycogen synthase and phosphorylase were studied in hepatocytes from fed rats. Elevation of extra-cellular glucose led to a rapid decrease in phosphorylase a activity followed by a slower increase in glycogen synthase I activity. A reciprocal and c...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00596a004
更新日期:1978-02-07 00:00:00
abstract::In vitro transcription assays have been used to study the rate of ribonucleic acid (RNA) synthesis from the Escherichia coli lactose promotor mutant lacL8UV5 contained on a 203-bp (base pair) restriction fragment. The half-life of long (63-base) RNA production from heparin-resistant RNA polymerase-promotor complexes w...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00511a003
更新日期:1981-04-14 00:00:00
abstract::The effects of various ginsenosides on calmodulin-dependent phosphodiesterase isozymes have been investigated. Ginsenosides were found to be potent inhibitors of bovine heart calmodulin-dependent phosphodiesterase and the 60-kDa isozyme of bovine brain calmodulin-dependent phosphodiesterase but not of the 63-kDa isozy...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00070a001
更新日期:1993-05-18 00:00:00
abstract::The solution conformation of the ribonuclease barnase has been determined by using 1H nuclear magnetic resonance (NMR) spectroscopy. The 20 structures were calculated by using 853 interproton distance restraints obtained from analyses of two-dimensional nuclear Overhauser spectra, 72 phi and 53 chi 1 torsion angle res...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00099a030
更新日期:1991-09-03 00:00:00
abstract::Factor V and factor Va binding to single bilayer phospholipid vesicles was investigated by light-scattering intensity measurements. This technique allows the measurement of free and phospholipid-bound protein concentrations from which equilibrium constants can be obtained. As controls, the Ca2+-dependent phospholipid ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00587a023
更新日期:1979-10-02 00:00:00
abstract::Pancreatic and duodenal homeobox 1 (Pdx1) is a homeodomain transcription factor belonging to the ParaHox family. Pdx1 plays an essential role in pancreatic endocrine and exocrine cell development and maintenance of adult islet beta-cell function. Mutations in the human pdx1 gene are linked to an early onset form of no...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi060969l
更新日期:2007-03-20 00:00:00
abstract::The red pigments in meat products, including cooked cured ham, arise from the reaction of myoglobin with nitric oxide generated from exogenous nitrite. Since carcinogenic nitrosoamines may be generated by the treatment of meats with nitrite, the production of nitrite-free meat products is an attractive alternative. Ra...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi701460x
更新日期:2007-12-25 00:00:00