Abstract:
:When stoichiometric amounts of tropomyosin (TM) are bound to F-actin in the presence of 2 mM ATP, the MG2+-activated acto-heavy meromyosin (HMM) ATPase is inhibited by about 60% in 5 mM MgCl2-30 mM KCl. If the concentration of MgCl2 is reduced to 1 mM, the inhibition disappears because TM no longer binds to F-actin. Increasing the concentration of KCl to 100 mM restores both the binding and the inhibition. Thus, the binding of TM alone to F-actin causes significant inhibition of the ATPase provided that the HMM is saturated with ATP. (When the HMM is not saturated, TM activates the ATPase). When TM alone can bind stoichiometrically to F-actin, addition of troponin I (TN-I) increases the inhibition from 60% to about 85%, but the TM binding to F-actin is not affected. Under conditions such that TM alone neither inhibits the acto-HMM ATPase nor binds to F-actin, the inhibition caused by TN-I plus TM still approaches 100%. Direct binding studies under these conditions show that TN-I induces binding between TM and F-actin. A dual role for TN-I is proposed: first, TN-I can induce TM to bind to F-actin, causing inhibition of the ATPase; and second, TN-I can itself enhance the inhibition of the ATPase in a cooperative manner. The addition of TN-C in the absence of CA2+ has only a limited effect on the first role, but seems to be able to block completely the cooperative inhibition caused by TN-I such that the residual inhibition is a function only of the TM which remains bound.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Eaton BL,Kominz DR,Eisenberg Edoi
10.1021/bi00683a025subject
Has Abstractpub_date
1975-06-17 00:00:00pages
2718-25issue
12eissn
0006-2960issn
1520-4995journal_volume
14pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::Intrastrand cross-links (IaCL) connecting two purine nucleobases in DNA pose a challenge to high-fidelity replication in the cell. Various repair pathways or polymerase bypass can cope with these lesions. The influence of the phosphodiester linkage between two neighboring 2'-deoxyguanosine (dG) residues attached throu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.5b01078
更新日期:2015-12-29 00:00:00
abstract::The L-type Ca(2+) channel Ca(v)1.2 forms macromolecular signaling complexes that comprise the β(2) adrenergic receptor, trimeric G(s) protein, adenylyl cyclase, and cAMP-dependent protein kinase (PKA) for efficient signaling in heart and brain. The protein phosphatases PP2A and PP2B are part of this complex. PP2A coun...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi101018c
更新日期:2010-12-07 00:00:00
abstract::The solution of a primary 16S rRNA-binding ribosomal protein, S17, was investigated by two- and three-dimensional homonuclear and heteronuclear magnetic resonance spectroscopy. Almost complete chemical shift assignments for the 1H, 15N, and 13C resonances have been obtained. The NMR data have been rigorously analyzed ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi951062i
更新日期:1996-03-05 00:00:00
abstract::In mesophiles, triosephosphate isomerase (TIM) is an obligated homodimer. We have previously shown that monomeric folding intermediates are common in the chemical unfolding of TIM, where dissociation provides 75% of the overall conformational stability of the dimer. However, analysis of the crystallographic structure ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi801360k
更新日期:2008-11-04 00:00:00
abstract::Phototropin, a blue-light photoreceptor in plants, has two FMN-binding domains named LOV1 and LOV2. We previously observed temperature-dependent FTIR spectral changes in the C=O stretching region (amide-I vibrational region of the peptide backbone) for the LOV2 domain of Adiantum phytochrome3 (phy3-LOV2), suggesting p...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi061837v
更新日期:2006-12-26 00:00:00
abstract::In the present study hydrostatic pressure was applied upon both skeletal myosin rod molecules and rod minifilaments to learn more of the intra- and intermolecular interaction behavior of myosin. Applied pressure disassembled the rod minifilaments into individual rod molecules and dissociated each myosin rod molecule i...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00184a028
更新日期:1994-05-10 00:00:00
abstract::The synthesis of sphingomyelin was studied in baby hamster kidney cells and in subcellular fractions derived from rat liver. During pulse-chase experiments with [3H]choline in tissue culture cells, the specific radioactivity of sphingomyelin continued to increase after the specific activities of phosphocholine and cyt...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00540a027
更新日期:1982-05-25 00:00:00
abstract::Prolyl oligopeptidase B from Galerina marginata (GmPOPB) has recently been discovered as a peptidase capable of breaking and forming peptide bonds to yield a cyclic peptide. Despite the relevance of prolyl oligopeptidases in human biology and disease, a kinetic analysis pinpointing rate-limiting steps for a member of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.7b00012
更新日期:2017-04-18 00:00:00
abstract::MantATP [2'(3')-O-(-N-methylanthraniloyl)-adenosine 5'-triphosphate] was employed as a fluorescence probe of the nucleotide-binding sites of dynein from sea urchin sperm flagella. MantATP binds specifically with enhanced fluorescence (approximately 2.2-fold), homogeneous lifetime (8.4 ns), and high anisotropy (r appro...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9730184
更新日期:1998-07-07 00:00:00
abstract::Common structural motifs, such as the cupin domains, are found in enzymes performing different biochemical functions while retaining a similar active site configuration and structural scaffold. The soil bacterium Bacillus subtilis has 20 cupin genes (0.5% of the total genome) with up to 14% of its genes in the form of...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0484421
更新日期:2005-01-11 00:00:00
abstract::Pump-1 cDNA has recently been isolated by screening a human tumor cDNA library with a transin (rat stromelysin) probe under low-stringency hybridization conditions. The cDNA codes for a potential protein with significant sequence similarity to the metalloproteinases collagenase and stromelysin, but which lacks the hem...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00439a004
更新日期:1989-06-27 00:00:00
abstract::Reconstitution of highly purified aquaporin CHIP (channel-forming integral protein) into proteoliposomes was previously shown to confer high osmotic water permeability (Pf) to the membranes [Zeidel et al. (1992) Biochemistry 31, 7436-7440]. Here we report detailed ultrastructural, pharmacologic, and transport studies ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00172a042
更新日期:1994-02-15 00:00:00
abstract::The effects of various ginsenosides on calmodulin-dependent phosphodiesterase isozymes have been investigated. Ginsenosides were found to be potent inhibitors of bovine heart calmodulin-dependent phosphodiesterase and the 60-kDa isozyme of bovine brain calmodulin-dependent phosphodiesterase but not of the 63-kDa isozy...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00070a001
更新日期:1993-05-18 00:00:00
abstract::The recA locus of pathogenic mycobacteria differs from that of nonpathogenic species because it contains large intervening sequences nested in the RecA homology region that are excised by an unusual protein-splicing reaction. In vivo assays indicated that Mycobacterium tuberculosis recA partially complemented Escheric...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9517751
更新日期:1996-02-13 00:00:00
abstract::The crystal structure of a ternary complex of the alcohol dehydrogenase from the archaeon Sulfolobus solfataricus (SsADH) has been determined at 2.3 A. The asymmetric unit contains a dimer with a NADH and a 2-ethoxyethanol molecule bound to each subunit. The comparison with the apo structure of the enzyme reveals that...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi035271b
更新日期:2003-12-16 00:00:00
abstract::The unfolding and dissociation of the tetrameric enzyme fructose-1,6-bisphosphatase from pig kidney by guanidine hydrochloride have been investigated at equilibrium by monitoring enzyme activity, ANS binding, intrinsic (tyrosine) protein fluorescence, exposure of thiol groups, fluorescence of extrinsic probes (AEDANS,...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi034203m
更新日期:2003-06-17 00:00:00
abstract::A chromatin-bound protease, active in 2 M NaCl-5 M urea or 5 M urea alone, was demonstrated in rat liver, kidney, testes, brain, rabbit bone marrow, chicken reticulocyte, and Ehrlich ascites chromatin. Chicken erythrocyte chromatin did not possess any detectable proteolytic activity in salt and urea. The proteolytic a...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00646a028
更新日期:1976-01-13 00:00:00
abstract::The 1.9 A X-ray crystal structure of human myeloperoxidase complexed with cyanide (R = 0.175, R(free) = 0.215) indicates that cyanide binds to the heme iron with a bent Fe-C-N angle of approximately 157 degrees, and binding is accompanied by movement of the iron atom by 0.2 A into the porphyrin plane. The bent orienta...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi0111808
更新日期:2001-11-20 00:00:00
abstract::The tyrosine kinase c-Abl is implicated in a variety of cellular processes that are tightly regulated by c-Abl kinase activity and/or by interactions between c-Abl and other signaling molecules. The interaction of c-Abl with the Abl interactor protein Abi2 is shown to be negatively regulated by phosphorylation of seri...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi701533j
更新日期:2008-01-22 00:00:00
abstract::The solution structure and dynamics of human insulin are investigated by 2D 1H NMR spectroscopy in reference to a previously analyzed analogue, des-pentapeptide(B26-B30) insulin (DPI; Hua, Q.X., & Weiss, M.A. (1990) Biochemistry 29, 10545-10555). This spectroscopic comparison is of interest since (i) the structure of ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00236a025
更新日期:1991-06-04 00:00:00
abstract::Assignments have been made for 11 methyl, one Gln-C gamma, one Thr-C beta, and all six Tyr-C zeta carbon resonances of ribonuclease A. These partially serve to delineate the binding sites for Cu2+, Mn2+, phosphate, cytidine and its 2'-, 3'-, and 5'-phosphates (Cyd and Cyd-2'-P, -3'-P, and -5'-P), and one or a few urea...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00310a020
更新日期:1984-07-17 00:00:00
abstract::NMR spectroscopy and simulated annealing calculations have been used to determine the three-dimensional structure of RK-1, an antimicrobial peptide from rabbit kidney recently discovered from homology screening based on the distinctive physicochemical properties of the corticostatins/defensins. RK-1 consists of 32 res...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi000457l
更新日期:2000-12-26 00:00:00
abstract::cAMP binding of the androgen receptor (AR) from murine skeletal muscle was studied. Testosterone affinity chromatography yielded androgen receptor with about 4000-fold purification. Determination of the cAMP binding in the affinity eluate, by adsorption of protein-cAMP complexes to cellulose ester filters or removal o...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00423a013
更新日期:1988-11-15 00:00:00
abstract::3-(Trifluoromethyl)-3-(m-iodophenyl)diazirine (TID) has recently been found to be a noncompetitive inhibitor of the nicotinic acetylcholine receptor (nAcChoR) by both photolabeling and flux assays (White et al., 1991). However, these experiments were done when TID was in equilibrium with the nAcChoR, and thus only its...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00255a018
更新日期:1994-12-27 00:00:00
abstract::An S3 electron paramagnetic resonance (EPR) signal is observed in a variety of photosystem 2 (PS2) samples in which the oxygen-evolving complex (OEC) has been inhibited. These signals have been proposed to be due to an interaction, S2X+, between the manganese cluster in an oxidation state equivalent to S2 and an organ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00088a032
更新日期:1993-09-21 00:00:00
abstract::Neutral solutes were used to investigate the effects of osmotic stress both on the ability of rhodopsin to undergo its activating conformation change and on acyl chain packing in the rod outer segment (ROS) disk membrane. The equilibrium concentration of metarhodopsin II (MII), the conformation of photoactivated rhodo...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi990634m
更新日期:1999-06-15 00:00:00
abstract::Glu58 is known to participate in phosphodiester transesterification catalyzed by the enzyme RNase T1. For Glu58 RNase T1, an altered mechanism has been proposed in which His40 replaces Glu58 as the base catalyst [Steyaert, J., Hallenga, K., Wyns, L., & Stanssens, P. (1990) Biochemistry 29, 9064-9072]. Glu58Ala Rnase T...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00173a006
更新日期:1994-02-22 00:00:00
abstract::The role of the 2'-hydroxyl group in RNA--protein interaction has been investigated using MS2 coat protein and its hairpin RNA operator as a model system. Derivatives of the MS2 translational operator were prepared where individual riboses were replaced by deoxyribose and their binding affinities to MS2 coat protein w...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00038a033
更新日期:1995-09-26 00:00:00
abstract::A cross-linked tripeptide has been isolated from alkaline hydrolysates of NaB3H4-reduced calf bone collagen. The peptide contains dihydroxylysinonorleucine, the most abundant cross-link in bone collagen, and it has a single N-terminal proline and a single C-terminal valine. These amino acids are in peptide linkage wit...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00691a011
更新日期:1975-10-07 00:00:00
abstract::A 15-residue fragment within the major continuous domain of gp120 from HIV-1 that can bind independently to the CD4 receptor has been shown to have the property of behaving as a solvent polarity-dependent conformational switch. The switch behavior (cooperative transition from beta-sheet to helical conformation as a fu...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi952045w
更新日期:1996-01-16 00:00:00