Structural, Functional, and Dynamical Responses of a Protein in a Restricted Environment Imposed by Macromolecular Crowding.

abstract：:The inhibitory effect of phosphorothioate residues, located within one strand of double-stranded DNA, on the hydrolytic activity of the restriction endonuclease EcoRV was investigated. Specific incorporation of a phosphorothioate group at the site of cleavage yielded the sequence 5'-GATsATC-3'. This modified sequence ...

journal_title：Biochemistry

authors： Olsen DB,Kotzorek G,Eckstein F

更新日期：1990-10-16 00:00:00

abstract：:Macromolecular protease inhibitors and camelid single-domain antibodies achieve their enzymic inhibition functions often through protruded structures that directly interact with catalytic centers of targeted proteases. Inspired by this phenomenon, we constructed synthetic human antibody libraries encoding long CDR-H3s...

journal_title：Biochemistry

authors： Nam DH,Lee KB,Kruchowy E,Pham H,Ge X

更新日期：2020-10-13 00:00:00

abstract：:The phytocyanins are a family of plant cupredoxins that have been subdivided into the stellacyanins, plantacyanins, and uclacyanins. All of these proteins possess the typical type 1 His(2)Cys equatorial ligand set at their mononuclear copper sites, but the stellacyanins have an axial Gln ligand in place of the weakly ...

journal_title：Biochemistry

authors： Harrison MD,Yanagisawa S,Dennison C

更新日期：2005-03-01 00:00:00

abstract：:The effect of anions on the stability of different functional conformations of Hb is examined through the determination of the dependence of O(2) affinity on water activity (a(w)). The control of a(w) is effected by varying the sucrose osmolal concentration in the bathing solution according to the "osmotic stress" met...

journal_title：Biochemistry

authors： Colombo MF,Seixas FA

更新日期：1999-09-07 00:00:00

abstract：:The relationship between steroid binding and protein subunit interactions of rabbit sex steroid binding protein (rSBP) has been studied by steady-state and time-resolved fluorescence spectroscopy. The high-affinity (Ka approximately 10(8) M-1 at 4 degrees C), fluorescent estrogen d-1,3,5(10),6,8-estrapentaene-3,17 bet...

journal_title：Biochemistry

authors： Casali E,Petra PH,Ross JB

更新日期：1990-10-09 00:00:00

abstract：:The kinetic mechanism of NADPH-cytochrome P450 oxidoreductase (P450R) has been determined for the model reaction with cytochrome c3+. Although initial velocity studies show parallel patterns, consistent with a classical (one-site) ping-pong mechanism that precludes the formation of a ternary NADPH-P450R-cytochrome c3+...

journal_title：Biochemistry

authors： Sem DS,Kasper CB

更新日期：1994-10-11 00:00:00

abstract：:Escherichia coli PurK, a dimeric N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) synthetase, catalyzes the conversion of 5-aminoimidazole ribonucleotide (AIR), ATP, and bicarbonate to N5-CAIR, ADP, and Pi. Crystallization of both a sulfate-liganded and the MgADP-liganded E. coli PurK has resulted in structures at 2....

journal_title：Biochemistry

authors： Thoden JB,Kappock TJ,Stubbe J,Holden HM

更新日期：1999-11-23 00:00:00

abstract：:The filamentous coliphage M13 possesses multiple copies of a 50-residue coat protein which is inserted into the inner membrane of Escherichia coli during infection. 13C nuclear magnetic resonance (NMR) spectroscopy has been used to probe the structure and dynamics of M13 coat protein solubilized in detergent micelles....

journal_title：Biochemistry

authors： Henry GD,Weiner JH,Sykes BD

更新日期：1986-02-11 00:00:00

abstract：:The CCA-adding enzymes [ATP(CTP):tRNA nucleotidyl transferases] catalyze synthesis of the conserved and essential CCA sequence to the tRNA 3' end. These enzymes are divided into two classes of distinct structures that differ in the overall orientation of the head to tail domains. However, the catalytic core of the two...

journal_title：Biochemistry

authors： Hou YM,Gu SQ,Zhou H,Ingerman L

更新日期：2005-09-27 00:00:00

abstract：:Triglyceride analogues were synthesized in which one of the primary acyl ester functions has been replaced by an alkyl group and the secondary acyl ester bond has been replaced by an acyl amino bond. The chain length at either position was varied, and both (R)- and (S)-enantiomers of each compound were synthesized. Th...

journal_title：Biochemistry

authors： Mannesse ML,Cox RC,Koops BC,Verheij HM,de Haas GH,Egmond MR,van der Hijden HT,de Vlieg J

更新日期：1995-05-16 00:00:00

abstract：:Adenylosuccinate synthetase catalyzes the first committed step in the de novo biosynthesis of AMP, coupling L-aspartate and IMP to form adenylosuccinate. Km values of IMP and 2'-deoxy-IMP are nearly identical with each substrate supporting comparable maximal velocities. Nonetheless, the Km value for L-aspartate and th...

journal_title：Biochemistry

authors： Iancu CV,Zhou Y,Borza T,Fromm HJ,Honzatko RB

更新日期：2006-09-26 00:00:00

abstract：:The aldehyde oxidoreductase from Desulfovibrio gigas belongs to the family of molybdenum hydroxylases. Besides a molybdenum cofactor which constitutes their active site, these enzymes contain two [2Fe-2S](2+,1+) clusters which are believed to transfer the electrons provided by the substrate to an acceptor which is eit...

journal_title：Biochemistry

authors： More C,Asso M,Roger G,Guigliarelli B,Caldeira J,Moura J,Bertrand P

更新日期：2005-08-30 00:00:00

abstract：:To better understand the mechanism of nitric oxide (NO) synthesis, we studied conversion of N-hydroxy-L-arginine (NOHA) or L-arginine (Arg) to citrulline and NO under single-turnover conditions using the oxygenase domain of neuronal nitric oxide synthase (nNOSoxy) and rapid scanning stopped-flow spectroscopy. When ana...

journal_title：Biochemistry

authors： Boggs S,Huang L,Stuehr DJ

更新日期：2000-03-07 00:00:00

abstract：:In an effort to better understand protein-protein photoaffinity cross-linking using aryl azides, we have tested a number of factors influencing the cross-linking of the sigma 70 subunit of Escherichia coli RNA polymerase to core RNA polymerase. These factors include the effect of the incubations necessary for the deri...

journal_title：Biochemistry

authors： McMahan SA,Burgess RR

更新日期：1994-10-11 00:00:00

abstract：:The class I human liver alcohol dehydrogenases (ADHs) catalyze the interconversion of the intermediary alcohols and aldehydes of dopamine metabolism in vitro, whereas those of the class II and class III do not. The individual, homogeneous class I isozymes oxidize (3,4-dihydroxyphenyl)ethanol and (4-hydroxy-3-methoxyph...

journal_title：Biochemistry

authors： Mårdh G,Vallee BL

更新日期：1986-11-18 00:00:00

abstract：:The inhibitory potency of 3'-azido-3'-deoxythymidine 5'-triphosphate (AZTTP) against HIV-1 reverse transcriptase (HIV-1 RT) has been further evaluated. The results indicate that the previously reported low Ki values for AZTTP against HIV-1 RT (2.35 nM) are due neither to the to the direct tight binding of AZTTP to HIV...

journal_title：Biochemistry

authors： Ma QF,Bathurst IC,Barr PJ,Kenyon GL

更新日期：1992-02-11 00:00:00

abstract：:The photoreceptor protein rhodopsin has been reconstituted with a single phospholipid species, dimyristoylphosphatidylcholine, at a range of different lipid/protein ratios, and the exchange rate at the lipid-protein interface has been determined from the electron spin resonance spectra of spin-labeled phosphatidylchol...

journal_title：Biochemistry

authors： Ryba NJ,Horváth LI,Watts A,Marsh D

更新日期：1987-06-02 00:00:00

abstract：:In the globin family, similarities in the folding mechanism have been found among different mammalian apomyoglobins (apoMb). The best-characterized intermediate of sperm whale apoMb, called I(AGH), is mainly stabilized by nativelike contacts among the A, G, and H helices involving a cluster of hydrophobic residues tha...

journal_title：Biochemistry

authors： Musto R,Bigotti MG,Travaglini-Allocatelli C,Brunori M,Cutruzzolà F

更新日期：2004-01-13 00:00:00

abstract：:The interaction of fluorescent ATP analog 2'(3')-O-[N-[2-[3-(5-fluoresceinyl)thioureido]-ethyl]carbamoyl]adenosine 5'-triphosphate (FEDA-ATP) with rabbit skeletal myosin subfragment 1 (S1) and acto-S1 was studied. This and related ATP analogs are potentially useful for determination of the ATPase activity of single my...

journal_title：Biochemistry

authors： Conibear PB,Jeffreys DS,Seehra CK,Eaton RJ,Bagshaw CR

更新日期：1996-02-20 00:00:00

abstract：:Several diseases, such as cancer, are characterized by acidification of the extracellular environment. Acidosis can be employed as a target to specifically direct therapies to the diseased tissue. We have used first principles to design an acidity-triggered rational membrane (ATRAM) peptide with high solubility in sol...

journal_title：Biochemistry

authors： Nguyen VP,Alves DS,Scott HL,Davis FL,Barrera FN

更新日期：2015-11-03 00:00:00

abstract：:Ion channel peptides have been prepared by solid-phase peptide synthesis, labeled with 15N at selected sites, and reconstituted into oriented lipid bilayers. The (Leu-Ser-Ser-Leu-Leu-Ser-Leu)3-CONH2 peptide has previously been shown to exhibit well-defined and discrete ionic conductances when investigated by single-ch...

journal_title：Biochemistry

authors： Sudheendra US,Bechinger B

更新日期：2005-09-13 00:00:00

abstract：:Light-harvesting complex II (LHC-II) regulates the light energy distribution between photosystem I and II in plants. This process is mediated by phosphorylation of the LHC-II protein, which depends on the oxidation state of photosynthetic electron carriers. In addition to this regulatory mechanism, it has recently bee...

journal_title：Biochemistry

authors： Rogl H,Kühlbrandt W,Barth A

更新日期：2003-09-02 00:00:00

abstract：:On the basis of the available high-resolution structures of mandelate racemase (MR) from Pseudomonas putida [Landro, J. A., Gerlt, J. A., Kozarich, J. W., Koo, C. W., Shah, V. J., Kenyon, G. L., Neidhart, D. J., Fujita, J., & Petsko, G. A. (1994) Biochemistry 33, 635-643], Lys 166 and His 297 are positioned appropriat...

journal_title：Biochemistry

authors： Kallarakal AT,Mitra B,Kozarich JW,Gerlt JA,Clifton JG,Petsko GA,Kenyon GL

更新日期：1995-03-07 00:00:00

abstract：:Pig kidney general acyl-CoA dehydrogenases forms the blue neutral radical on dithionite or photochemical reduction (Thorpe, C., Matthews, R. G., & Williams, C. H. (1979) Biochemistry 18, 331-337] in accord with its classification as a flavoprotein dehydrogenase. However, dithionite reduction of the enzyme in the prese...

journal_title：Biochemistry

authors： Mizzer JP,Thorpe C

更新日期：1981-08-18 00:00:00

abstract：:Numerous studies have concluded that the interaction of the amyloid β-peptide (Aβ) and cellular membranes contributes to the toxicity and cell death observed in the progression of Alzheimer's disease. Aggregated Aβ species disrupt membranes, leading to physical instability and ion leakage. Further, the presence of Aβ ...

journal_title：Biochemistry

authors： Lemkul JA,Bevan DR

更新日期：2013-07-23 00:00:00

abstract：:The tyrosine kinase c-Abl is implicated in a variety of cellular processes that are tightly regulated by c-Abl kinase activity and/or by interactions between c-Abl and other signaling molecules. The interaction of c-Abl with the Abl interactor protein Abi2 is shown to be negatively regulated by phosphorylation of seri...

journal_title：Biochemistry

authors： Jung JH,Pendergast AM,Zipfel PA,Traugh JA

更新日期：2008-01-22 00:00:00

abstract：:A high degree of selectivity toward the target site of the pest organism is a desirable attribute for new safer agrochemicals. To assist in the design of novel herbicides, we determined the crystal structures of the herbicidal target enzyme 4-hydroxyphenylpyruvate dioxygenase (HPPD; EC 1.13.11.27) from the plant Arabi...

journal_title：Biochemistry

authors： Yang C,Pflugrath JW,Camper DL,Foster ML,Pernich DJ,Walsh TA

更新日期：2004-08-17 00:00:00

abstract：:DREAM (also known as K(+) channel interacting protein 3 and calsenilin) is a calcium binding protein and an active modulator of KV4 channels in neuronal cells as well as a novel Ca(2+)-regulated transcriptional modulator. DREAM has also been associated with the regulation of Alzheimer's disease through the prevention ...

journal_title：Biochemistry

authors： Gonzalez WG,Ramos V,Diaz M,Garabedian A,Molano-Arevalo JC,Fernandez-Lima F,Miksovska J

更新日期：2016-03-29 00:00:00

abstract：:Messenger RNA degradation is an important component of overall gene expression. During the final step of eukaryotic mRNA degradation, exoribonuclease 1 (Xrn1) carries out 5' → 3' processive, hydrolytic degradation of RNA molecules using divalent metal ion catalysis. To initiate studies of the 5' → 3' RNA decay machine...

journal_title：Biochemistry

authors： Langeberg CJ,Welch WRW,McGuire JV,Ashby A,Jackson AD,Chapman EG

更新日期：2020-04-21 00:00:00

abstract：:We have used limited proteolysis of affinity-labeled estrogen receptors (ER), coupled with antireceptor antibody immunoreactivity, to assess structural features of ER and the relatedness of ER from MCF-7 human breast cancer and rat uterine cells. MCF-7 ER preparations covalently labeled with [3H]tamoxifen aziridine [(...

journal_title：Biochemistry

authors： Katzenellenbogen BS,Elliston JF,Monsma FJ Jr,Springer PA,Ziegler YS,Greene GL

更新日期：1987-04-21 00:00:00