Structure of the major carbohydrate fragment of the Leishmania donovani lipophosphoglycan.

abstract：:Detection of specific reaction products is a powerful approach for dissecting out pathways that mediate oxidative damage in vivo. Eosinophil peroxidase (EPO), an abundant protein secreted from activated eosinophils, has been implicated in promoting oxidative tissue injury in conditions such as asthma, allergic inflamm...

journal_title：Biochemistry

authors： Wu W,Chen Y,d'Avignon A,Hazen SL

更新日期：1999-03-23 00:00:00

abstract：:Modulation of endogenous gene function, through sequence-specific recognition of double helical DNA via oligonucleotide-directed triplex formation, is a promising approach. Compared to the formation of pyrimidine motif triplexes, which require relatively low pH, purine motif appears to be the most gifted for their sta...

journal_title：Biochemistry

authors： Kaushik S,Kaushik M,Svinarchuk F,Malvy C,Fermandjian S,Kukreti S

更新日期：2011-05-17 00:00:00

abstract：:The roles of four of the active center groups with potential acid-base properties in the region of pH optimum of pyruvate decarboxylase from Saccharomyces cerevisiae have been studied with the substitutions Asp28Ala, His114Phe, His115Phe, and Glu477Gln, introduced by site-directed mutagenesis methods. The steady-state...

journal_title：Biochemistry

authors： Liu M,Sergienko EA,Guo F,Wang J,Tittmann K,Hübner G,Furey W,Jordan F

更新日期：2001-06-26 00:00:00

abstract：:The murine invariant chain (Ii) was found to incorporate radioactive palmitic acid. This binding of fatty acid inhibits the formation of interchain S-S bonds, probably because the cysteine residue in the transmembrane region of the Ii chain is palmitylated. The inhibition of fatty acylation by cerulenin blocks further...

journal_title：Biochemistry

authors： Koch N,Hämmerling GJ

更新日期：1985-10-22 00:00:00

abstract：:Time-resolved spectroscopic studies of unfolded horse iron(II) cytochrome c have suggested that the imidazole side chains of His26 and His33 bind transiently to the heme iron on microsecond time scales, after photodissociation of a carbon monoxide ligand from the heme. Our studies of four variants of cytochrome c (hor...

journal_title：Biochemistry

authors： Hagen SJ,Latypov RF,Dolgikh DA,Roder H

更新日期：2002-01-29 00:00:00

abstract：:The technique of quasi-elastic light scattering was used to measure the translational diffusion coefficient, D, of purified human prothrombin in buffered aqueous solutions and to monitor for the first time the fragmentation of this protein as it is converted to thrombin. The values of D20,w, measured at two different ...

journal_title：Biochemistry

authors： Agarwal GP,Gallagher JG,Aune KC,Armeniades CD

更新日期：1977-05-03 00:00:00

abstract：:Transport of hydrophilic di- and tripeptides into Lactococcus lactis is mediated by a proton motive force-driven peptide transport protein (DtpT) that shares similarity with eukaryotic peptide transporters, e.g., from kidney and small intestine of rabbit, man, and rat. Hydropathy profiling in combination with the "pos...

journal_title：Biochemistry

authors： Hagting A,vd Velde J,Poolman B,Konings WN

更新日期：1997-06-03 00:00:00

abstract：:Earlier thermodynamic studies of the intermolecular interactions between mature oxytocin and neurophysin, and of the effects of these interactions on neurophysin folding, raised questions about the intramolecular interactions of oxytocin with neurophysin within their common precursor. To address this issue, the disulf...

journal_title：Biochemistry

authors： Eubanks S,Lu M,Peyton D,Breslow E

更新日期：1999-10-12 00:00:00

abstract：:The effect of substituting Pro-L209 with Tyr, Phe, Glu, and Thr in photosynthetic reaction centers (RCs) from Rhodobacter sphaeroides was investigated by monitoring the light-induced FTIR absorption changes associated with the photoreduction of the secondary quinone Q(B). Pro-L209 is close to a chain of ordered water ...

journal_title：Biochemistry

authors： Nabedryk E,Breton J,Sebban P,Baciou L

更新日期：2003-05-20 00:00:00

abstract：:The 19-transmembrane multisubunit γ-secretase complex generates the amyloid β-peptide (Aβ) of Alzheimer's disease (AD) by intramembrane proteolysis of the β-amyloid precursor protein (APP). Despite substantial advances in elucidating how this protein complex functions, the effect of the local membrane lipid microenvir...

journal_title：Biochemistry

authors： Holmes O,Paturi S,Ye W,Wolfe MS,Selkoe DJ

更新日期：2012-05-01 00:00:00

abstract：:The nature of the active site cofactor and the amino acid sequence flanking this structure have been determined in a range of copper amine oxidases. For enzymes from porcine plasma, porcine kidney, and pea seedlings, proteolytic digestion was performed on phenylhydrazone or p-nitrophenylhydrazone derivatives. Thermoly...

journal_title：Biochemistry

authors： Janes SM,Palcic MM,Scaman CH,Smith AJ,Brown DE,Dooley DM,Mure M,Klinman JP

更新日期：1992-12-08 00:00:00

abstract：:Several models for how amino acid substitutions in the Plasmodium falciparum chloroquine resistance transporter (PfCRT) confer resistance to chloroquine (CQ) and other antimalarial drugs have been proposed. Distinguishing between these models requires detailed analysis of high-resolution CQ transport data that is unfo...

journal_title：Biochemistry

authors： Cabrera M,Natarajan J,Paguio MF,Wolf C,Urbach JS,Roepe PD

更新日期：2009-10-13 00:00:00

abstract：:Although there is considerable evidence that signaling by the erythropoietin (EPO) receptor is initiated when it is dimerized by binding EPO, it has been previously reported that the soluble extracellular domains of the EPO receptor (sEPOR) are not dimerized in the presence of EPO and are able to form only 1:1 complex...

journal_title：Biochemistry

authors： Philo JS,Aoki KH,Arakawa T,Narhi LO,Wen J

更新日期：1996-02-06 00:00:00

abstract：:In an effort to gain greater insight into the molecular mechanism of the electron-transfer reactions of cytochrome b(5), the bovine cytochrome b(5)-horse cytochrome c complex has been investigated by high-resolution multidimensional NMR spectroscopy using (13)C, (15)N-labeled cytochrome b(5) expressed from a synthetic...

journal_title：Biochemistry

authors： Hom K,Ma QF,Wolfe G,Zhang H,Storch EM,Daggett V,Basus VJ,Waskell L

更新日期：2000-11-21 00:00:00

abstract：:Detailed analysis of factors governing high affinity antibody-antigen interactions yields important insight into molecular recognition and facilitates the design of functional antibody libraries. Here we describe comprehensive mutagenesis of the light chain complementarity determining regions (CDRs) of HIV-1 antibody ...

journal_title：Biochemistry

authors： Da Silva GF,Harrison JS,Lai JR

更新日期：2010-07-06 00:00:00

abstract：:Cytochrome b(561) in adrenal chromaffin vesicle membranes conveys electron equivalents from extravesicular ascorbate to the intravesicular monodehydroascorbate radical. We conducted a stopped-flow study on the reaction of ascorbate with purified cytochrome b(561) in the detergent-solubilized state for the first time. ...

journal_title：Biochemistry

authors： Takigami T,Takeuchi F,Nakagawa M,Hase T,Tsubaki M

更新日期：2003-07-15 00:00:00

abstract：:cis peptide bonds in proteins are often rate-limiting steps in protein folding or conformational change and are frequently stabilized by metal ions. In the collagen-binding domain of Clostridium histolyticum collagenase, the binding of calcium ions triggers the formation of a cis peptide bond. We present free energy s...

journal_title：Biochemistry

authors： Spiriti J,van der Vaart A

更新日期：2010-06-29 00:00:00

abstract：:The technique of fluorescence recovery after photobleaching is used to perform an extensive study of the lateral diffusion of a phospholipid probe in the binary mixture dimyristoylphosphatidylcholine/cholesterol, above the melting temperature of the phospholipid. In the regions of the phase diagram where a single liqu...

journal_title：Biochemistry

authors： Almeida PF,Vaz WL,Thompson TE

更新日期：1992-07-28 00:00:00

abstract：:Sphingosine, sphinganine, and other long-chain (sphingoid) bases inhibit protein kinase C in vitro and block cellular responses to agonists that are thought to act via this enzyme. To gain further insight into the mechanism of this inhibition, a series of long-chain analogues differing in alkyl chain length (11-20 car...

journal_title：Biochemistry

authors： Merrill AH Jr,Nimkar S,Menaldino D,Hannun YA,Loomis C,Bell RM,Tyagi SR,Lambeth JD,Stevens VL,Hunter R

更新日期：1989-04-18 00:00:00

abstract：:Cytochrome c binds to cardiolipin (CL) on the inner mitochondrial membrane during the initial stages of apoptosis where it oxidizes CL, promoting its release into the cytoplasm where it initiates apoptosis. Previous work has identified interaction sites on cytochrome c involved in the cytochrome c-CL interaction. The ...

journal_title：Biochemistry

authors： Elmer-Dixon MM,Bowler BE

更新日期：2018-10-02 00:00:00

abstract：:Light-induced activation of the LOV2-Jα domain of the photoreceptor phototropin from oat is believed to involve the detachment of the Jα helix from the central β-sheet and its subsequent unfolding. The dynamics of these conformational changes were monitored by time-resolved emission spectroscopy with 100 ns time resol...

journal_title：Biochemistry

authors： Hoersch D,Bolourchian F,Otto H,Heyn MP,Bogomolni RA

更新日期：2010-12-28 00:00:00

abstract：:We have measured the base-pair lifetimes in oligodeoxynucleotides containing tracts of A.T base pairs using imino proton magnetic resonance. When the tract contains more than four consecutive A.T base pairs, possibly including a 5'-AT step but not a 5'-TA step, anomalously long lifetimes are observed. For example, the...

journal_title：Biochemistry

authors： Leroy JL,Charretier E,Kochoyan M,Guéron M

更新日期：1988-12-13 00:00:00

abstract：:Chemical modification and proteolytic digestion studies have identified a transmembrane glutamic acid residue (E953) of the alpha subunit of the pig kidney Na, K-ATPase as a possible cation binding site [Goldshleger et al. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 6911-6915]. In addition, an adjacent glutamate (E954) i...

journal_title：Biochemistry

authors： Van Huysse JW,Jewell EA,Lingrel JB

更新日期：1993-01-26 00:00:00

abstract：:Tissue factor pathway inhibitor (TFPI) from different cell lines shows up to 15-fold differences in the ratio of anticoagulant to chromogenic activity. The anticoagulant activity was dependent on the purification procedure used and it was possible to isolate two fractions of recombinant TFPI. Only one of these fractio...

journal_title：Biochemistry

authors： Nordfang O,Bjørn SE,Valentin S,Nielsen LS,Wildgoose P,Beck TC,Hedner U

更新日期：1991-10-29 00:00:00

abstract：:The dynamic equilibrium of a catalytic site between active and inactive conformations, the missing link between the structure and function of allosteric enzymes, was identified using protein engineering and NMR techniques. Kinetic analyses of the wild-type and three mutants of Thermus L-lactate dehydrogenase establish...

journal_title：Biochemistry

authors： Koide S,Yokoyama S,Matsuzawa H,Miyazawa T,Ohta T

更新日期：1992-06-16 00:00:00

abstract：:Lactose repressor protein, regulator of lac enzyme expression in Escherichia coli, maintains its structure and function at extremely low protein concentrations (<10(-)12 M). To examine the unfolding and dissociation of this tetrameric protein, structural transitions in the presence of varying concentrations of urea we...

journal_title：Biochemistry

authors： Barry JK,Matthews KS

更新日期：1999-05-18 00:00:00

abstract：:In the Arc repressor dimer, the side chains of Ile37 and Val41 in alpha-helix B pack against each other and against the symmetry-related side chains of Ile37' and Val41' in alpha-helix B' to form part of the hydrophobic core and the dimer interface. Following combinatorial mutagenesis of these positions, only the wild...

journal_title：Biochemistry

authors： Milla ME,Sauer RT

更新日期：1995-03-14 00:00:00

abstract：:RecA-catalyzed DNA recombination is initiated by a mandatory, high-energy form of DNA in RecA-nucleoprotein filaments, where bases are highly unstacked and the backbone is highly unwound. Interestingly, only the energetics consequent to adenosine triphosphate (ATP) binding, rather than its hydrolysis, seems sufficient...

journal_title：Biochemistry

authors： Ramreddy T,Sen S,Rao BJ,Krishnamoorthy G

更新日期：2003-10-21 00:00:00

abstract：:A detailed structural study of the prokaryotic sodium/galactose transporter (vSGLT) from Vibrio parahaemolyticus using attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy reveals stepwise increases in alpha-helicity upon binding of sodium and D-galactose. These increases in helicity correlat...

journal_title：Biochemistry

authors： le Coutre J,Turk E,Kaback HR,Wright EM

更新日期：2002-06-25 00:00:00

abstract：:The ability of cell-penetrating peptides (CPPs) to cross cell membranes has found numerous applications in the delivery of bioactive compounds to the cytosol of living cells. Their internalization mechanisms have been questioned many times, and after 20 years of intense debate, it is now widely accepted that both ener...

journal_title：Biochemistry

authors： Di Pisa M,Chassaing G,Swiecicki JM

更新日期：2015-01-20 00:00:00