Abstract：
:The technique of fluorescence recovery after photobleaching is used to perform an extensive study of the lateral diffusion of a phospholipid probe in the binary mixture dimyristoylphosphatidylcholine/cholesterol, above the melting temperature of the phospholipid. In the regions of the phase diagram where a single liquid phase exists, diffusion can be quantitatively described by free volume theory, using a modified Macedo-Litovitz hybrid equation. In the liquid-liquid immiscibility region, the temperature dependence of the diffusion coefficient is in excellent agreement with current theories of generalized diffusivities in composite two-phase media. A consistent interpretation of the diffusion data can be provided based essentially on the idea that the primary effect of cholesterol addition to the bilayer is to occupy free volume. On this basis, a general interpretation of the phase behavior of this mixture is also proposed.
journal_name
Biochemistryjournal_title
Biochemistryauthors
Almeida PF,Vaz WL,Thompson TEdoi
10.1021/bi00144a013subject
Has Abstractpub_date
1992-07-28 00:00:00pages
6739-47issue
29eissn
0006-2960issn
1520-4995journal_volume
31pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The association of ferredoxin with ferredoxin:NADP+ reductase (both proteins from spinach chloroplasts) was characterized by isothermal titration calorimetry and fluorescence quenching titration. The formation of the complex is mainly driven by a positive entropy change (delta S = 125 +/- 8 J mol-1 K-1). The calorimet...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00249a019
更新日期：1994-11-15 00:00:00
abstract：:A 35-amino acid peptide corresponding to the putative "zinc finger" sequence of primase was prepared to study its zinc binding properties. When zinc was added to the peptide, it was found that the fluorescence quantum yield of the single tyrosine increased by 46% and the average lifetime by 34%. The binding stoichiome...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9612778
更新日期：1997-01-21 00:00:00
abstract：:The epidermal growth factor receptor (EGFR) kinase is activated by a variety of mutations in human cancers. R776H is one such recurrent mutation (R752H in another numbering system) in the αC-β4 loop of the tyrosine kinase domain that activates EGFR in the absence of the activating EGF ligand. However, the mechanistic ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00444
更新日期：2015-07-14 00:00:00
abstract：:Complementary DNA clones coding for the human secreted carbonic anhydrase isozyme (CA VI) have been isolated and their nucleotide sequences determined. These clones identify a 1.45-kb mRNA that is present in high levels in parotid submandibular salivary glands but absent in other tissues such as the sublingual gland, ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00216a035
更新日期：1991-01-15 00:00:00
abstract：:The field of chemical site-selective modification of proteins has progressed extensively in recent decades to enable protein functionalization for imaging, drug delivery, and functional studies. In this Perspective, we provide detailed insight into an alternative use of site-selective protein chemistry to probe the ro...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b00639
更新日期：2018-10-30 00:00:00
abstract：:The dimeric two-component system transmitter protein NRII (NtrB) of Escherichia coli, product of glnL (ntrB), controls transcription of nitrogen-regulated genes by catalyzing the phosphorylation and dephosphorylation of the transcription factor NRI (NtrC). Previous studies showed that the PII signal transduction prote...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi000794u
更新日期：2000-11-07 00:00:00
abstract：:3-Fluorooxalacetate is a substrate for malic dehydrogenase. When enzymatic reduction is slower than the rate of epimerization of the two enantiomers, only (2R,3R)-erythro-fluoromalate is formed. Conversely, when a high enzyme level and excess of NADH lead to reduction that is fast relative to the epimerization rate, e...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi981819g
更新日期：1998-12-22 00:00:00
abstract：:Purified subunits of intermediate filaments obtained from a variety of tissues and cell types contain O-phosphoserine and, in some cases, smaller amounts of O-phosphothreonine. The O-phosphoserine content was estimated by reaction of performic acid oxidized subunits with methylamine in NaOH. Decamin of BHK-21 and CHO ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00530a030
更新日期：1982-01-05 00:00:00
abstract：:The trimeric main light-harvesting complex (LHC-II) is the only antenna complex of higher plants of which a high-resolution 3D structure has been obtained (Kühlbrandt, W., Wang, D., and Fujiyoshi, Y. (1994) Nature 367, 614-621) and which can be refolded in vitro from its components. Four different recombinant forms of...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi015875k
更新日期：2002-02-19 00:00:00
abstract：:FtmPT1 is a fungal indole prenyltransferase that catalyzes the reaction of tryptophan derivatives with dimethylallyl pyrophosphate to form various biologically active compounds. Herein, we describe detailed studies of FtmPT1 catalysis involving dimethylallyl pyrophosphate and Brevianamide F following the native pathwa...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi500747z
更新日期：2014-09-30 00:00:00
abstract：:The dark reaction of tris(hydroxymethyl)aminomethane (Tris) with the O2-evolving center of photosystem II (PSII) in the S1 state causes irreversible inhibition of O2 evolution. Similar inhibition is observed for several other amines: NH3, CH3NH2, (CH3)2NH, ethanolamine, and 2-amino-2-ethyl-1,3-propanediol. In PSII mem...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00246a003
更新日期：1991-08-13 00:00:00
abstract：:The active site topography of rabbit CYP4B1 has been studied relative to CYP2B1 and CYP102 using a variety of aromatic probe substrates. Oxidation of the prochiral substrate cumene by CYP4B1, but not CYP2B1 or CYP102, resulted in the formation of the thermodynamically disfavored omega-hydroxy metabolite, 2-phenyl-1-pr...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi010395e
更新日期：2001-07-24 00:00:00
abstract：:We found that peroxisomal lignoceroyl-CoA ligase, like palmitoyl-CoA ligase, is present in the peroxisomal membrane whereas the peroxisomal beta-oxidation enzyme system is localized in the matrix. To further define the role of peroxisomal acyl-CoA ligases (membrane component) in providing acyl-CoA for peroxisomal beta...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00468a027
更新日期：1990-04-24 00:00:00
abstract：:In this report we provide biochemical evidence that a highly purified synaptic plasma membrane fraction derived from rat brain, after intraventricular injection of 35S-labeled sodium sulfate, is enriched in a number of large sulfated glycoproteins compared with a purified myelin fraction studied concurrently. A fracti...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00670a005
更新日期：1976-12-14 00:00:00
abstract：:This paper presents the crystal structure of porcine pancreatic carboxypeptidase B (pp-CpB) in complex with a variety of thiol-based inhibitors that were developed as antagonists of activated thrombin-activatable fibrinolysis inhibitor (TAFIa). Recent studies have indicated that a selective inhibitor of TAFIa could en...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0501941
更新日期：2005-07-05 00:00:00
abstract：:NMR spectroscopy is particularly adept at site-specifically monitoring dynamic processes in proteins, such as protein folding, domain movements, ligand binding, and side-chain rotations. By coupling the favorable spectroscopic properties of highly dynamic side-chain methyl groups with transverse-relaxation-optimized s...
journal_title：Biochemistry
pub_type： 杂志文章,评审
doi：10.1021/acs.biochem.8b00953
更新日期：2019-01-22 00:00:00
abstract：:While the urea-mediated unfolding pathway of the Escherichia coli aspartate aminotransferase (eAATase) homodimer proceeds through a reversible three-state process with a partially folded dimeric intermediate, D D* 2U (E. Deu and J. F. Kirsch, accompanying paper), that of a cofactor-stabilized form differs. Pyridoxal p...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi602632d
更新日期：2007-05-15 00:00:00
abstract：:1-Aminocyclopropanephosphonate (ACPP) was synthesized, and its effects on the pyridoxal 5'-phosphate linked enzymes 1-aminocyclopropanecarboxylate (ACPC) deaminase from Pseudomonas sp. ACPC and alanine racemase from Bacillus stearothermophilus were studied. ACPP was found to be a potent inhibitor of both enzymes with ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00386a025
更新日期：1987-06-16 00:00:00
abstract：:Fragile X syndrome, the most common cause of inherited mental retardation, is caused by the absence of the fragile X mental retardation protein (FMRP). The emerging picture is that FMRP is involved in repression of translation through a complex network of protein-protein and protein-RNA interactions. Very little struc...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi034909g
更新日期：2003-09-09 00:00:00
abstract：:The equilibrium binding of hemoglobin to isolated band 3 protein exhibited positive cooperativity [Hill coefficient = 1.65 +/- 0.1; total number of binding sites at pH 6.6 in 5 mM sodium phosphate buffer = 32 500 +/- 940 pmol/mg; Ka = (3.0 +/- 0.5) X 10(5) M-1]. The binding was reversible and ionic in nature as the bo...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00359a054
更新日期：1986-06-03 00:00:00
abstract：:The alpha-helices of coiled-coil proteins are predominantly parallel, in contrast to the general preference for an antiparallel orientation of interacting alpha-helices found in globular proteins. One intriguing exception is the antiparallel, two-stranded coiled coil comprising the long helical arm of the bacterial se...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi962391t
更新日期：1997-03-04 00:00:00
abstract：:The cytochrome P450-dependent terminal desaturation of valproic acid (VPA) is of both toxicological and mechanistic interest because the product, 4-ene-VPA, is a more potent hepatotoxin than the parent compound and its generation represents a rather novel metabolic reaction for the cytochrome P450 system. In the prese...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00024a013
更新日期：1995-06-20 00:00:00
abstract：:Maltosyl isothiocyanate (MITC) has been synthesized from maltose with an overall yield of 88%. It has been found to be a potent irreversible inhibitor of zero trans influx of glucose with human erythrocytes. Kinetic analysis of glucose transport after treatment of erythrocytes with MITC revealed that VT was diminished...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00547a025
更新日期：1980-03-18 00:00:00
abstract：:We have studied alkylation of the acetylcholine receptor by N-[3H]ethylmaleimide ([3H]NEM) under various conditions. The radiolabeled preparations were submitted to sodium dodecyl sulfate-polyacrylamide gel electrophoresis to separate the receptor complex into subunits, and the incorporation of 3H into each type of ch...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00306a001
更新日期：1984-05-22 00:00:00
abstract：:Complete resonance assignments of nonexchangeable base protons and sugar protons in d-GGTACGCGTACC at 500 MHz have been obtained by two-dimensional correlated spectroscopy (COSY) and nuclear Overhauser enhancement spectroscopy (NOESY). The characteristic phase-sensitive multiplet patterns of the ntrasugar cross peaks ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00410a050
更新日期：1988-05-17 00:00:00
abstract：:The chromatin structure encompassing the lysozyme gene domain in hen oviduct nuclei was studied by measuring the partitioning of coding and flanking sequences during chromatin fractionation and by analyzing the nucleosome repeat in response to micrococcal nuclease digestion. Following micrococcal nuclease digestion, n...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00350a033
更新日期：1986-01-28 00:00:00
abstract：:A chromatin-bound protease, active in 2 M NaCl-5 M urea or 5 M urea alone, was demonstrated in rat liver, kidney, testes, brain, rabbit bone marrow, chicken reticulocyte, and Ehrlich ascites chromatin. Chicken erythrocyte chromatin did not possess any detectable proteolytic activity in salt and urea. The proteolytic a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00646a028
更新日期：1976-01-13 00:00:00
abstract：:DNA polymerase β (Pol β) is a key enzyme in mammalian base excision repair (BER), contributing stepwise 5'-deoxyribose phosphate (dRP) lyase and "gap-filling" DNA polymerase activities. The lyase reaction is believed to occur via a β-elimination reaction following the formation of a Schiff base between the dRP group a...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.6b00807
更新日期：2017-01-24 00:00:00
abstract：:Factor VIII (FVIII) is the nonproteolytic cofactor for FIXa in the tenase complex of blood coagulation. FVIII is proteolytically activated by thrombin and FXa in vitro to form a heterotrimer with full procoagulant activity. Activated protein C inactivates thrombin-activated FVIII through cleavage adjacent to position ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00125a022
更新日期：1992-03-17 00:00:00
abstract：:Rh(phen)2phi3+ (phen = 1,10-phenanthroline; phi = 9,10-phenanthrenequinone diimine) avidly binds to DNA via intercalation from the major groove and upon photoactivation produces strand scission with single base 5' asymmetry. Enantiomers of Rh(phen)2phi3+, which lack hydrogen-bonding substituents in ancillary positions...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00180a005
更新日期：1994-04-12 00:00:00
