当前位置： SCI文献检索 > BIOCHEMISTRY期刊下所有文献 > Evidence that the principal CoII-binding site in human serum albumin is not at the N-terminus: implication on the albumin cobalt binding test for detecting myocardial ischemia.

Evidence that the principal CoII-binding site in human serum albumin is not at the N-terminus: implication on the albumin cobalt binding test for detecting myocardial ischemia.

Abstract：

:Human serum albumin (HSA) is the most abundant protein in the blood plasma and is involved in the transport of metal ions. Four metal-binding sites with different specificities have been described in HSA: (i) the N-terminal site provided by Asp1, Ala2, and His3, (ii) the site at the reduced Cys34, (iii) site A, including His67 as a ligand, and (iv) the nonlocalized site B. HSA can bind CoII, and HSA was proposed to be involved in CoII transport. Recently, binding of CoII to HSA has attracted much interest due to the so-called albumin cobalt binding (ACB) test approved by the Food and Drug Administration for evaluation of myocardial ischemia. Although the binding of CoII to HSA is important, the binding of CoII to HSA is not well-characterized. Here the binding of CoII to HSA was studied under anaerobic conditions to prevent CoII oxidation. Electronic absorption, EPR, and NMR spectroscopies indicate three specific and well-separated binding sites for CoII in HSA. CoII ions in all three sites are in a high-spin state and coordinated in a distorted octahedral geometry. Competition experiments with CdII (known to bind to sites A and B) and CuII (known to bind to the N-terminal site) were used to identify the sites of binding of CoII to HSA. They revealed that the first two equivalents of CoII bind to sites A and B. Only the third may be bound to the N-terminal site. The repercussions of these results on the understanding of the ACB test and hence the myocardial ischemia are discussed.

journal_name

Biochemistry

journal_title

Biochemistry

authors

Mothes E,Faller P

doi

10.1021/bi061783p

subject

Has Abstract

pub_date

2007-02-27 00:00:00

pages

2267-74

issue

8

eissn

0006-2960

issn

1520-4995

journal_volume

46

pub_type

杂志文章

相关文献

BIOCHEMISTRY文献大全
  • 1H NMR assignment and global fold of napin BnIb, a representative 2S albumin seed protein.

    abstract：:Napin BnIb is a representative member of the 2S albumin seed proteins, which consists of two polypeptide chains of 3.8 and 8.4 kDa linked by two disulfide bridges. In this work, a complete assignment of the 1H spectra of napin BnIb has been carried out by two-dimensional NMR sequence-specific methods and its secondary...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi961748q

    authors： Rico M,Bruix M,González C,Monsalve RI,Rodríguez R

    更新日期：1996-12-10 00:00:00

  • Composition and structure of zinc-deficient Euglena gracilis chromatin.

    abstract：:The histone content of zinc-deficient (-Zn) Euglena gracilis decreases while, concomitantly, DNA content increases and the transcription rate is reduced markedly [Mazus, B., Falchuk, K. H., & Vallee, B. L. (1983) Biochemistry (in press); Falchuk, K. H., Fawcett, D. W., & Vallee, B. L. (1975) J. Cell Sci. 17, 57-78]. T...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00291a014

    authors： Stankiewicz AJ,Falchuk KH,Vallee BL

    更新日期：1983-10-25 00:00:00

  • In vitro analysis of carboxyacyl substrate tolerance in the loading and first extension modules of borrelidin polyketide synthase.

    abstract：:The borrelidin polyketide synthase (PKS) begins with a carboxylated substrate and, unlike typical decarboxylative loading PKSs, retains the carboxy group in the final product. The specificity and tolerance of incorporation of carboxyacyl substrate into type I PKSs have not been explored. Here, we show that the first e...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi500951c

    authors： Hagen A,Poust S,de Rond T,Yuzawa S,Katz L,Adams PD,Petzold CJ,Keasling JD

    更新日期：2014-09-30 00:00:00

  • Interfacial catalysis by phospholipase A2: the rate-limiting step for enzymatic turnover.

    abstract：:The kinetics of the phospholipase A2-catalyzed hydrolysis of bilayer vesicles and mixed micelles of several oxyglycero and thioglycero analogues of phospholipids have been studied. The results with vesicles show that, depending on the source of the enzyme, the rates of hydrolysis of the oxy-containing long-chain phosp...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00149a014

    authors： Jain MK,Yu BZ,Rogers J,Gelb MH,Tsai MD,Hendrickson EK,Hendrickson HS

    更新日期：1992-09-01 00:00:00

  • Abortive initiation and long ribonucleic acid synthesis.

    abstract：:In vitro transcription assays have been used to study the rate of ribonucleic acid (RNA) synthesis from the Escherichia coli lactose promotor mutant lacL8UV5 contained on a 203-bp (base pair) restriction fragment. The half-life of long (63-base) RNA production from heparin-resistant RNA polymerase-promotor complexes w...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00511a003

    authors： Munson LM,Reznikoff WS

    更新日期：1981-04-14 00:00:00

  • Rapid Oxidation Following Photoreduction in the Avian Cryptochrome4 Photocycle.

    abstract：:Avian magnetoreception is assumed to occur in the retina. Although its molecular mechanism is unclear, magnetic field-dependent formation and the stability of radical-containing photointermediate(s) are suggested to play key roles in a hypothesis called the radical pair mechanism. Chicken cryptochrome4 (cCRY4) has bee...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.0c00495

    authors： Otsuka H,Mitsui H,Miura K,Okano K,Imamoto Y,Okano T

    更新日期：2020-09-29 00:00:00

  • Site-directed mutagenesis and 1H NMR spectroscopy of an interdomain segment in the pyruvate dehydrogenase multienzyme complex of Escherichia coli.

    abstract：:Deletion of two of the three homologous lipoyl domains that form the N-terminal half of each dihydrolipoamide acetyltransferase (E2p) polypeptide chain of the Escherichia coli pyruvate dehydrogenase complex can be achieved by in vitro deletion in the structural gene aceF. A site-directed mutagenesis of this shortened ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00401a044

    authors： Texter FL,Radford SE,Laue ED,Perham RN,Miles JS,Guest JR

    更新日期：1988-01-12 00:00:00

  • The intrinsic protein flexibility of endogenous protease inhibitor TIMP-1 controls its binding interface and affects its function.

    abstract：:Protein flexibility is thought to play key roles in numerous biological processes, including antibody affinity maturation, signal transduction, and enzyme catalysis, yet only limited information is available regarding the molecular details linking protein dynamics with function. A single point mutation at the distal s...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi902141x

    authors： Grossman M,Tworowski D,Dym O,Lee MH,Levy Y,Murphy G,Sagi I

    更新日期：2010-07-27 00:00:00

  • Structure-activity relationships of piscidin 4, a piscine antimicrobial peptide.

    abstract：:Piscidin 4, an antimicrobial peptide recently isolated from mast cells of hybrid striped bass (Morone chrysops female × Morone saxatilis male), is unusual in that it is twice as long (44 amino acids) as the typical members of the piscidin family. We previously showed that native piscidin 4 had a modified amino acid at...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi101395j

    authors： Park NG,Silphaduang U,Moon HS,Seo JK,Corrales J,Noga EJ

    更新日期：2011-04-26 00:00:00

  • Mutational analysis of the roles in catalysis and substrate recognition of arginines 54 and 305, aspartic acid 309, and tryptophan 317 located at subsites 1 and 2 in glucoamylase from Aspergillus niger.

    abstract：:The mutants Arg54-->Leu, Arg54-->Lys, Arg305-->Lys, Asp309-->Glu, and Trp317-->Phe, located at subsites 1 and 2 in glucoamylase from Aspergillus niger, provide insight into the importance of specific hydrogen bonds and hydrophobic interactions in substrate recognition, catalytic mechanism, and stability. As suggested ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00032a009

    authors： Frandsen TP,Christensen T,Stoffer B,Lehmbeck J,Dupont C,Honzatko RB,Svensson B

    更新日期：1995-08-15 00:00:00

  • Identification of protein side chains near the membrane-aqueous interface: a site-directed spin labeling study of KcsA.

    abstract：:This study presents an approach to identifying surface residues on membrane proteins that are exposed toward the membrane-aqueous interface. The method employs a lipid Ni(II) chelate that localizes the metal ion to a region near the membrane-aqueous interface. Lateral diffusion of the lipid chelate results in Heisenbe...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi015828s

    authors： Gross A,Hubbell WL

    更新日期：2002-01-29 00:00:00

  • The p73 DNA binding domain displays enhanced stability relative to its homologue, the tumor suppressor p53, and exhibits cooperative DNA binding.

    abstract：:The p53 protein family is involved in the control of an intricate network of genes implicated in cell cycle, through to germ line integrity and development. Although the role of p53 is well-established, the intrinsic nature of its homologue p73 has yet to be fully elucidated. Here, the biochemical characterization and...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi7023207

    authors： Patel S,Bui TT,Drake AF,Fraternali F,Nikolova PV

    更新日期：2008-03-11 00:00:00

  • Mapping the interfacial binding surface of human secretory group IIa phospholipase A2.

    abstract：:Human secretory group IIa phospholipase A2 (hIIa-PLA2) contains a large number of prominent cationic patches on its molecular surface and has exceptionally high affinity for anionic surfaces, including anionic membranes. To identify the cationic amino acid residues that support binding of hIIa-PLA2 to anionic membrane...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi971200z

    authors： Snitko Y,Koduri RS,Han SK,Othman R,Baker SF,Molini BJ,Wilton DC,Gelb MH,Cho W

    更新日期：1997-11-25 00:00:00

  • Structural insight into how Streptomyces coelicolor maltosyl transferase GlgE binds α-maltose 1-phosphate and forms a maltosyl-enzyme intermediate.

    abstract：:GlgE (EC 2.4.99.16) is an α-maltose 1-phosphate:(1→4)-α-d-glucan 4-α-d-maltosyltransferase of the CAZy glycoside hydrolase 13_3 family. It is the defining enzyme of a bacterial α-glucan biosynthetic pathway and is a genetically validated anti-tuberculosis target. It catalyzes the α-retaining transfer of maltosyl units...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi500183c

    authors： Syson K,Stevenson CE,Rashid AM,Saalbach G,Tang M,Tuukkanen A,Svergun DI,Withers SG,Lawson DM,Bornemann S

    更新日期：2014-04-22 00:00:00

  • High-resolution crystal structures of the lectin-like xylan binding domain from Streptomyces lividans xylanase 10A with bound substrates reveal a novel mode of xylan binding.

    abstract：:Carbohydrate-binding module (CBM) family 13 includes the "R-type" or "ricin superfamily" beta-trefoil lectins. The C-terminal CBM, CBM13, of xylanase 10A from Streptomyces lividans is a family 13 CBM that is not only structurally similar to the "R-type" lectins but also somewhat functionally similar. The primary funct...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi015865j

    authors： Notenboom V,Boraston AB,Williams SJ,Kilburn DG,Rose DR

    更新日期：2002-04-02 00:00:00

  • How does a DNA interacting enzyme change its specificity during molecular evolution? A site-directed mutagenesis study at the DNA binding site of the DNA-(adenine-N6)-methyltransferase EcoRV.

    abstract：:The EcoRV DNA-(adenine-N6)-methyltransferase (MTase) recognizes GATATC sequences and modifies the first adenine residue within this site. Parts of its DNA interface show high sequence homology to DNA MTases of the dam family which recognize and modify GATC sequences. A phylogenetic analysis of M.EcoRV and dam-MTases s...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0155450

    authors： Beck C,Cranz S,Solmaz M,Roth M,Jeltsch A

    更新日期：2001-09-18 00:00:00

  • Heparin prevents the binding of phospholipase A2 to phospholipid micelles: importance of the amino-terminus.

    abstract：:The activity of the major isoform of porcine pancreatic phospholipase A2 (PLA2), designated B-PLA2, against micellar substrates is inhibited by heparin. Inhibition is a consequence of binding of the enzyme to heparin, documented by a heparin-induced alteration in the intrinsic fluorescence of B-PLA2 and in the 8-anili...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00101a026

    authors： Diccianni MB,Lilly-Stauderman M,McLean LR,Balasubramaniam A,Harmony JA

    更新日期：1991-09-17 00:00:00

  • Inhibition of neuronal sodium and potassium ion activated adenosinetriphosphatase by pyrithiamin.

    abstract：:Since one of the electrophysiological effects of pyrithiamin, an antimetabolite of thiamin, suggested an interference with sodium pump mechanisms, the effect of pyrithiamin on Na+,K+-ATPase was investigated. We found that whereas preincubation of the antimetabolite with nonneuronal preparations of Na+,K+-ATPase produc...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00278a023

    authors： Matsuda T,Cooper JR

    更新日期：1983-04-26 00:00:00

  • Effect of Different Divalent Cations on the Kinetics and Fidelity of RB69 DNA Polymerase.

    abstract：:Although Mg(2+) is the cation that functions as the cofactor for the nucleotidyl transfer reaction for almost all DNA polymerases, Mn(2+) can also serve, but when it does, the degree of base discrimination exhibited by most DNA polymerases (pols) is diminished. Metal ions other than Mg(2+) or Mn(2+) can also act as co...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.5b01350

    authors： Vashishtha AK,Konigsberg WH

    更新日期：2016-05-10 00:00:00

  • Molecular dynamics simulations of the unfolding of barnase in water and 8 M aqueous urea.

    abstract：:Molecular dynamics simulations of barnase have been conducted both in water and in 8 M urea solution for 500 ps at 25 degrees C and for 2000 ps at 85 degrees C. The final structure of the aqueous simulation at room temperature matches closely the structure obtained by NMR and the experimentally observed protections fr...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi970096i

    authors： Tirado-Rives J,Orozco M,Jorgensen WL

    更新日期：1997-06-17 00:00:00

  • Rapid changes in the phosphoproteome show diverse cellular responses following stimulation of human lung fibroblasts with endothelin-1.

    abstract：:The rapid phosphorylation and dephosphorylation of a variety of proteins downstream of the endothelin receptors A and B was investigated following stimulation of human lung fibroblasts with endothelin-1. Changes in the phosphorylation of proteins involved in the cell cycle, cytoskeleton, membrane channels, transcripti...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi035414u

    authors： Stannard C,Soskic V,Godovac-Zimmermann J

    更新日期：2003-12-02 00:00:00

  • Substrate-Induced Facilitated Dissociation of the Competitive Inhibitor from the Active Site of O-Acetyl Serine Sulfhydrylase Reveals a Competitive-Allostery Mechanism.

    abstract：:By classical competitive antagonism, a substrate and competitive inhibitor must bind mutually exclusively to the active site. The competitive inhibition of O-acetyl serine sulfhydrylase (OASS) by the C-terminus of serine acetyltransferase (SAT) presents a paradox, because the C-terminus of SAT binds to the active site...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.7b00500

    authors： Singh AK,Ekka MK,Kaushik A,Pandya V,Singh RP,Banerjee S,Mittal M,Singh V,Kumaran S

    更新日期：2017-09-19 00:00:00

  • Isolation and characterization of papaya peptidase A from commercial chymopapain.

    abstract：:Chromatography on a column of SP-Sephadex shows that commercial chymopapain contains three components with proteolytic activity. Each behaves as a single protein upon rechromatography and electrophoresis on polyacrylamide gels. The major component, which represents 31% of the activity applied to the column and is the ...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00687a028

    authors： Robinson GW

    更新日期：1975-08-12 00:00:00

  • Binding of calcium to anticoagulant protein S: role of the fourth EGF module.

    abstract：:Protein S is an anticoagulant protein containing a Gla (enclosing gamma-carboxyglutamic acids) module, a TSR (thrombin sensitive region) module, four EGF (epidermal growth factor)-like modules, and a SHBG (sex hormone binding globulin)-like region. Protein S is a cofactor to activated protein C (APC) in the degradatio...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0601151

    authors： Persson KE,Stenflo J,Linse S,Stenberg Y,Preston RJ,Lane DA,Rezende SM

    更新日期：2006-09-05 00:00:00

  • Enzyme-induced strain/distortion in the ground-state ES complex in beta-lactamase catalysis revealed by FTIR.

    abstract：:Class A beta-lactamases hydrolyze penicillins and other beta-lactams via an acyl-enzyme catalytic mechanism. Ser70 is the active site nucleophile. By constructing the S70A mutant, which is unable to form the acyl-enzyme intermediate, it was possible to make stable ES complexes with various substrates. The stability of...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9928041

    authors： Hokenson MJ,Cope GA,Lewis ER,Oberg KA,Fink AL

    更新日期：2000-05-30 00:00:00

  • Acrylamide quenching of Trp phosphorescence in liver alcohol dehydrogenase: evidence of gated quencher penetration.

    abstract：:Notwithstanding the relevance of their biological function, slow motions in proteins, beyond the microsecond range, are still poorly understood and often elusive. We propose that acrylamide quenching of Trp phosphorescence of deeply buried residues, when extended over the entire accessible range of lifetime measuremen...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9009659

    authors： Strambini GB,Gonnelli M

    更新日期：2009-08-11 00:00:00

  • Recognition of ATGA sequences by the unfused aromatic dication DB293 forming stacked dimers in the DNA minor groove.

    abstract：:Furamidine and related diamidines represent a promising series of drugs active against widespread parasites, in particular the Pneumocystic carinii pathogen. In this series, the phenylfuranbenzimidazole diamidine derivative DB293 was recently identified as the first unfused aromatic dication capable of forming stacked...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi0108453

    authors： Bailly C,Tardy C,Wang L,Armitage B,Hopkins K,Kumar A,Schuster GB,Boykin DW,Wilson WD

    更新日期：2001-08-21 00:00:00

  • The complete amino acid sequence of the major component myoglobin of Amazon river dolphin (Inia geoffrensis).

    abstract：:The complete amino acid sequence of the major component myoglobin from Amazon River dolphin, Inia geoffrensis, was determined by specific cleavage of the protein to obtain large peptides which are readily degraded by the automatic sequencer. Three easily separable peptides were obtained by cleaving the protein with cy...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi00695a018

    authors： Dwulet FE,Bogardt RA,Jones BN,Lehman LD,Gurd FR

    更新日期：1975-12-02 00:00:00

  • Assembly of A beta amyloid protofibrils: an in vitro model for a possible early event in Alzheimer's disease.

    abstract：:Amyloid fibrils comprising primarily the peptides A beta 40 and A beta 42 are a defining feature of the Alzheimer's disease (AD) brain, and convergent evidence suggests that the process of their formation plays a central role in the AD pathogenic pathway. Elucidation of fibril assembly is critical for the discovery of...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/bi9904149

    authors： Harper JD,Wong SS,Lieber CM,Lansbury PT Jr

    更新日期：1999-07-13 00:00:00

  • The Interaction between the Third Type III Domain from Fibronectin and Anastellin Involves β-Strand Exchange.

    abstract：:Anastellin is a small recombinant fragment derived from the extracellular matrix protein fibronectin; it comprises the first type III (FN3) domain without the two N-terminal β-strands. It inhibits angiogenesis, tumor growth, and metastasis in mouse models and requires endogenous fibronectin for its in vivo anti-angiog...

    journal_title：Biochemistry

    pub_type： 杂志文章

    doi：10.1021/acs.biochem.7b00633

    authors： Stine JM,Ahl GJH,Schlenker C,Rusnac DV,Briknarová K

    更新日期：2017-09-05 00:00:00