Abstract:
:Frequency-domain fluorescence resonance energy transfer and anisotropy measurements were performed to characterize conformational dynamics of an analog of the RNase S-peptide (residues 1-20). Trp was used as a donor by replacing Phe 8, and a dansyl acceptor group was introduced at position 1 or 18. The distance-distribution parameters, half width of the distribution, end-to-end diffusion coefficient, and to some extent anisotropy decays were sensitive to changes in the S-peptide conformation. The observed mean distance of about 13-14 A between residues 1 and 8 in the presence of 50% TFE and when bound to RNase S-protein is in reasonable accord with the X-ray structure of RNase. The mean distance of 9.3 A between residues 8 and 18 in the presence of 50% TFE is, however, significantly smaller than 15.3 A found for the S-protein complex. The half-width of the distance distribution increased from about 9 to 18 A for residues 1-8 and from about 6 to 14 A for segment 8-18 with the loss of helical structure. The half-widths of 9 A in the case of 1-8 segment when peptide is helical suggests the presence of considerable conformational heterogeneity. Also, the 14 A half-width for segment 8-18 when it is random-coil is smaller than that expected for a random coil 11-residue segment. The donor-to-acceptor diffusion coefficients were less than 1 x 10(-7) cm2/s at 2 degrees C for both segments and increased to 1-2 x 10(-6) cm2/s at 35 degrees C.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Biochemistryjournal_title
Biochemistryauthors
Maliwal BP,Lakowicz JR,Kupryszewski G,Rekowski Pdoi
10.1021/bi00097a009subject
Has Abstractpub_date
1993-11-23 00:00:00pages
12337-45issue
46eissn
0006-2960issn
1520-4995journal_volume
32pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract::The physiological effects of anesthetics have been ascribed to their interaction with hydrophobic sites within functionally relevant CNS proteins. Studies have shown that volatile anesthetics compete for luciferin binding to the hydrophobic substrate binding site within firefly luciferase and inhibit its activity (Fra...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi800544d
更新日期:2008-09-09 00:00:00
abstract::The correlation of the UVRR nuW3 mode with the tryptophan chi(2,1) dihedral angle [Maruyama and Takeuchi (1995) J. Raman Spectrosc. 26, 319; Miura et al. (1989) J. Raman Spectrosc. 20, 667; Takeuchi (2003) Biopolymers 72, 305] has been extended to a full, 360 degrees rotation. The 3-fold periodicity of the relationshi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi801293v
更新日期:2009-03-31 00:00:00
abstract::The Ca2+-dependent regulation of smooth muscle actomyosin involves a myosin light chain kinase (ATP: myosin light chain phosphotransferase). It has been shown (Dabrowska, R., Aromatorio, D., Sherry, J.M.F., and Hartshorne, D.J. 1977, Biochem. Biophys. Res. Commun. 78, 1263) that the kinase is composed of two proteins ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00595a010
更新日期:1978-01-24 00:00:00
abstract::We show that eight different serine proteinases--bovine chymotrypsins A and B, porcine pancreatic elastase I, proteinase K, Streptomyces griseus proteinases A and B, and subtilisins BPN' and Carlsberg--interact with turkey ovomucoid third domain at the same Leu18-Glu19 peptide bond, the reactive site of the inhibitor....
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00341a007
更新日期:1985-09-24 00:00:00
abstract::The pathogenic bacteria Pseudomonas aeruginosa and Bordetella pertussis contain in their outer membranes the rare sugar 2,3-diacetamido-2,3-dideoxy-d-mannuronic acid. Five enzymes are required for the biosynthesis of this sugar starting from UDP-N-acetylglucosamine. One of these, referred to as WlbB, is an N-acetyltra...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi1005738
更新日期:2010-06-08 00:00:00
abstract::The association-dissociation kinetics of ribosomes from Escherichia coli have been studied under various conditions in a light-scattering stopped-flow apparatus. The dissociation reaction at 2 mg/ml at 25 degrees C, induced by lowering the MgCl2 concentration from 18 to 3 mM, can best be described by three independent...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00671a010
更新日期:1976-12-28 00:00:00
abstract::The cytochrome P450-dependent terminal desaturation of valproic acid (VPA) is of both toxicological and mechanistic interest because the product, 4-ene-VPA, is a more potent hepatotoxin than the parent compound and its generation represents a rather novel metabolic reaction for the cytochrome P450 system. In the prese...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00024a013
更新日期:1995-06-20 00:00:00
abstract::The cellular site of formation, Galpha-coupling preference, and agonist regulation of mu-delta opioid receptor (OR) heterooligomers were studied. Bioluminescence resonance energy transfer (BRET) showed that mu-deltaOR heterooligomers, composed of preformed mu and delta homooligomers, interacted constitutively in the e...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi701436w
更新日期:2007-11-13 00:00:00
abstract::The synthesis of 5-(2-oxalylethyl)-NADH, a reduced nicotinamide adenine dinucleotide (NADH) derivate with pyruvate covalently attached to the 5 position of the dihydronicotinamide ring over an additional methylene group has been described previously (Trommer, W.E., Blume, H., and Kapmeyer, H. (1976) Justus Liebigs Ann...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00668a012
更新日期:1976-11-16 00:00:00
abstract::The folate chromophore in native Escherichia coli DNA photolyase ([6R]-5,10-CH+-H4Pte(Glu)n=3-6) serves as an antenna, transferring light energy to the fully reduced flavin (FADH2) reaction center at high efficiency (EET = 0.92). Apophotolyase reconstituted after an overnight incubation with [6R,S]-5,10-CH+-H4folate (...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9605548
更新日期:1996-06-18 00:00:00
abstract::The transferrins (TFs) are a family of proteins that are widely distributed in vertebrates, where they serve a major role in iron binding and transport. Most TFs are composed of two homologous lobes, the N- and C-lobes, each able to bind a single iron atom. Human serum transferrin (hTF) binds iron in the blood and del...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi027071q
更新日期:2003-04-08 00:00:00
abstract::The distance between the calcium site (site I) and the zinc site (site II) in alpha-lactalbumin was estimated from Forster energy-transfer measurements between donor Eu(III) [or Tb(III)] at site I and acceptor Co(II) at site II to be 11.5 +/- 1.5 A. Intersite distances were also measured between the bis-ANS [4,4'-bis[...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00365a024
更新日期:1986-08-26 00:00:00
abstract::A systematic 220-MHz proton nuclear magnetic resonance (nmr) study has been made of all common purine and pyrimidine 2'(3')-ribonucleotides in D2O solutions at 20 plus or minus 2 degrees. Spectra for the entire series were measured under similar conditions of concentration, temperature, and ionic strength, thereby fac...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00674a013
更新日期:1975-02-11 00:00:00
abstract::In the Arc repressor dimer, the side chains of Ile37 and Val41 in alpha-helix B pack against each other and against the symmetry-related side chains of Ile37' and Val41' in alpha-helix B' to form part of the hydrophobic core and the dimer interface. Following combinatorial mutagenesis of these positions, only the wild...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00010a025
更新日期:1995-03-14 00:00:00
abstract::The crystal structure of the ternary complex citrate synthase-oxaloacetate-carboxymethyl coenzyme A has been solved to a resolution of 1.9 A and refined to a conventional crystallographic R factor of 0.185. The structure resembles a proposed transition state of the condensation reaction and suggests that the condensat...
journal_title:Biochemistry
pub_type: 杂志文章
doi:
更新日期:1990-03-06 00:00:00
abstract::The coexistence effects of multiple kinds of oxidized deoxyribonucleotides were examined using an SV40 origin-dependent in vitro replication system with a HeLa extract. Oxidized dGTP and dATP, 8-hydroxy-2'-deoxyguanosine 5'-triphosphate (8-OH-dGTP) and 2-hydroxy-2'-deoxyadenosine 5'-triphosphate (2-OH-dATP), were used...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi062117r
更新日期:2007-06-05 00:00:00
abstract::Matrix metalloproteinase-13 (MMP-13) is important in the pathology of osteoarthritis (OA). Although MMP-13 is considered a therapeutic target for OA, it is unclear how MMP-13 activity is regulated by the system that comprises various proteinases and their inhibitors. MMP-13 neutralizing antibodies could be a useful to...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi301228d
更新日期:2012-11-06 00:00:00
abstract::Aminoacyl-tRNA synthetases ensure the fidelity of protein synthesis by accurately selecting and activating cognate amino acids for aminoacylation of the correct tRNA. Some tRNA synthetases have evolved an editing active site that is separate from the amino acid activation site providing two steps or "sieves" for amino...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi002915w
更新日期:2001-05-08 00:00:00
abstract::The mechanism by which the heme-containing peroxidase, chloroperoxidase, is able to chlorinate substrates is poorly understood. One approach to advance our understanding of the mechanism of the enzyme is to determine those factors which contribute to its stability. In particular, under alkaline conditions, chloroperox...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi961512m
更新日期:1996-11-19 00:00:00
abstract::A 16-base pair oligo(purine)-oligo(pyrimidine) sequence present in the coding region of two HIV 1 proviral genes (pol and nef) was chosen as a target for triplex-forming oligonucleotides in in vitro transcription assays. Inhibition of transcription elongation was observed with triplex-forming oligonucleotide-acridine ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi952993x
更新日期:1996-08-13 00:00:00
abstract::HSC70 interacting protein (HIP) is an essential cytoplasmic cochaperone involved in the regulation of HSC70 chaperone activity and the maturation of progesterone receptor. To determine the quaternary structure and the gross conformation of the protein in solution, a wide array of biochemical and biophysical techniques...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi9917535
更新日期:2000-01-18 00:00:00
abstract::The interaction of poly[(G-C)] and poly[d(G-m5C)] with the antitumor antibiotic elsamicin A, which binds to alternating guanine + cytosine tracts in DNA, has been studied under the B and Z conformations. Both the rate and the extent of the B-to-Z transition are diminished by the antibiotic, as inferred by spectroscopi...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00161a051
更新日期:1992-11-24 00:00:00
abstract::Murine class alpha glutathione S-transferase subunit types A2 (mGSTA2-2) and A1 (mGSTA1-1) have high catalytic efficiency for glutathione (GSH) conjugation of the ultimate carcinogenic metabolite of benzo[a]pyrene, (+)-anti-7,8-dihydroxy-9,10-oxy-7,8,9,10-tetrahydrobenzo[a]pyrene, [(+)-anti-BPDE]. Only 10 residues dif...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi026778+
更新日期:2003-02-04 00:00:00
abstract::Comparative studies on the salt titration and the related kinetics for poly(dG-dC) X poly(dG-dC) in pH 7.0 and 3.8 solutions clearly suggest that base protonation facilitates the kinetics of B-Z interconversion although the midpoint for such a transition in acidic solution (2.0-2.1 M NaCl) is only slightly lower than ...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00320a041
更新日期:1984-12-04 00:00:00
abstract::Histidine-40 is known to participate in phosphodiester transesterification catalyzed by the enzyme ribonuclease T1. A mutant enzyme with a lysine replacing the histidine-40 (His40Lys RNase T1) retains considerable catalytic activity [Steyaert, J., Hallenga, K., Wyns, L., & Stanssens, P. (1990) Biochemistry 29, 9064-90...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00161a009
更新日期:1992-11-24 00:00:00
abstract::Glycosylases specifically recognize and flip their target base out of the DNA helix into the enzyme's active site. Our simulations show that a partially flipped state, already present in free DNA carrying a T:G mispair, becomes the more probable state compared to the closed state after binding of thymine DNA glycosyla...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/acs.biochem.8b00409
更新日期:2018-10-02 00:00:00
abstract::1,3-15N-Labeled uracil was synthesized chemically and used to prepare labeled Escherichia coli tRNA(Val) biosynthetically. 500-MHz measurements of 15N and proton chemical shift were obtained, for all uridine and uridine-related bases, by heteronuclear multiple-quantum coherence spectroscopy. All the uracil NH group re...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi00166a013
更新日期:1992-12-29 00:00:00
abstract::Temporins are short (10-13 amino acids) and linear antimicrobial peptides first isolated from the skin of the European red frog, Rana temporaria, and are effective against Gram-positive bacteria and Candida albicans. To get insight into their mechanism(s) of action, we compared the effects on model membranes exerted b...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi011929e
更新日期:2002-04-02 00:00:00
abstract::Following platelet activation, platelets undergo a dramatic shape change mediated by the actin cytoskeleton and accompanied by secretion of granule contents. While the actin cytoskeleton is thought to influence platelet granule secretion, the mechanism for this putative regulation is not known. We found that disruptio...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi100541t
更新日期:2010-06-01 00:00:00
abstract::Studies on the structure-function relationship of transporters require the availability of sufficient amounts of the protein in a functional state. In this paper, we report the functional expression, purification, and reconstitution of the human sodium/d-glucose cotransporter1 (hSGLT1) in Pichia pastoris and ligand-in...
journal_title:Biochemistry
pub_type: 杂志文章
doi:10.1021/bi051377q
更新日期:2005-11-29 00:00:00