Abstract：
:Fapy.dG is produced in DNA as a result of oxidative stress from a precursor that also forms OxodG. Bypass of Fapy.dG in a shuttle vector in COS-7 cells produces G --> T transversions slightly more frequently than does OxodG (Kalam, M. A., et al. (2006) Nucleic Acids Res. 34, 2305). The effect of Fapy.dG on replication in Escherichia coli was studied by transfecting M13mp7(L2) bacteriophage DNA containing the lesion within the lacZ gene in 4 local sequence contexts. For comparison, experiments were carried out side-by-side on OxodG. The efficiency of lesion bypass was determined relative to that of a genome containing native nucleotides. Fapy.dG was bypassed less efficiently than OxodG. Bypass efficiency of Fapy.dG and OxodG increased modestly in SOS-induced cells. Mutation frequencies at the site of the lesions in the originally transfected genomes were determined using the REAP assay (Delaney, J. C., Essigmann, J. M. (2006) Methods Enzymol. 408, 1). G --> T transversions were the only mutations observed above background when either Fapy.dG or OxodG was bypassed. OxodG mutation frequencies ranged from 3.1% to 9.8%, whereas the G --> T transversion frequencies observed upon Fapy.dG bypass were
journal_name
Biochemistryjournal_title
Biochemistryauthors
Patro JN,Wiederholt CJ,Jiang YL,Delaney JC,Essigmann JM,Greenberg MMdoi
10.1021/bi700628csubject
Has Abstractpub_date
2007-09-04 00:00:00pages
10202-12issue
35eissn
0006-2960issn
1520-4995journal_volume
46pub_type
杂志文章相关文献
BIOCHEMISTRY文献大全abstract：:The tyrosine kinase c-Abl is implicated in a variety of cellular processes that are tightly regulated by c-Abl kinase activity and/or by interactions between c-Abl and other signaling molecules. The interaction of c-Abl with the Abl interactor protein Abi2 is shown to be negatively regulated by phosphorylation of seri...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi701533j
更新日期：2008-01-22 00:00:00
abstract：:The membrane stabilization effect of cholesteryl hemisuccinate (CHEMS) and the sensitivity of the CHEMS-phosphatidylethanolamine membranes to protons and calcium ions were studied by differential scanning calorimetry, freeze-fracture electron microscopy, and 31P NMR. (1) At neutral pH, the addition of 8 mol % CHEMS to...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00328a013
更新日期：1985-03-26 00:00:00
abstract：:A general understanding of how cytochromes evolve within a fixed structure to optimize redox potential for specific bioenergetic processes does not exist. Toward this end, a library approach is used to investigate the range and distribution of redox potential which occurs when all sequence space available through muta...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi0001675
更新日期：2000-05-23 00:00:00
abstract：:The D/H fractionation factor (sigma) is the extent to which a hydrogen at a particular site becomes enriched in 2H over 1H relative to the solvent. A growing body of experimental evidence suggest that there is a correlation between the value of the fractionation factor and hydrogen-bond strength, with a lower sigma va...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00170a023
更新日期：1994-02-01 00:00:00
abstract：:A recently discovered bifunctional antibiotic-resistance enzyme named AAC(3)-Ib/AAC(6')-Ib', from Pseudomonas aeruginosa, catalyzes acetylation of aminoglycoside antibiotics. Since both domains are acetyltransferases, each was cloned and purified for mechanistic studies. The AAC(3)-Ib domain appears to be highly speci...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi700111z
更新日期：2007-05-01 00:00:00
abstract：:The thermal dependence of the structural stability of membrane-reconstituted yeast cytochrome c oxidase has been studied by using different techniques including high-sensitivity differential scanning calorimetry, differential detergent solubility thermal gel analysis, and enzyme activity measurements. For these studie...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00455a028
更新日期：1990-01-23 00:00:00
abstract：:Coordinate regulation of PARP-1 and -2 and PARG is required for cellular responses to genotoxic stress. While PARP-1 and -2 are regulated by DNA breaks and covalent modifications, mechanisms of PARG regulation are poorly understood. We report here discovery of a PARG regulatory segment far removed linearly from residu...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi100973m
更新日期：2010-09-07 00:00:00
abstract：:Photolyzed rhodopsin is phosphorylated at multiple serine and threonine residues during the quenching of phototransduction. Sites of phosphorylation by rhodopsin kinase have been localized to the C-terminal region of rhodopsin, but no information was available on the kinetics and identity of phosphorylated residues. T...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00072a030
更新日期：1993-06-01 00:00:00
abstract：:Platelet receptor recognition domains are located on the gamma and alpha chains of human fibrinogen. The former encompasses residues 400-411 [Kloczewiak, M., Timmons, S., Lukas, T. J., & Hawiger, J. (1984) Biochemistry 23, 1767], and the latter is present in two loci on the alpha chain (alpha 95-97 and alpha 572-574) ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00433a026
更新日期：1989-04-04 00:00:00
abstract：:Ner repressors of the transposable phages Mu and D108 play a central role in regulating the expression of the early (transposase) operon and in ensuring that phage growth proceeds along a lytic pathway. The latter function is analogous to that performed by the Cro protein of phage lambda. Unlike lambda Cro, however, t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00201a018
更新日期：1994-09-06 00:00:00
abstract：:Escherichia coli class Ib ribonucleotide reductase (RNR) converts nucleoside 5'-diphosphates to deoxynucleoside 5'-diphosphates in iron-limited and oxidative stress conditions. We have recently demonstrated in vitro that this RNR is active with both diferric-tyrosyl radical (Fe(III)(2)-Y(•)) and dimanganese(III)-Y(•) ...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi101881d
更新日期：2011-03-15 00:00:00
abstract：:An alanine racemase encoded by a gene from the thermophilic Gram-positive bacterium Bacillus stearothermophilus is overproduced to 0.3% of the soluble protein when carried on plasmid pICR4 in Escherichia coli [Inagaki, K., Tanizawa, K., Badet, B., Walsh, C. T., Tanaka, H., & Soda, K. (1986) Biochemistry (third paper o...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00359a029
更新日期：1986-06-03 00:00:00
abstract：:The thermodynamics of L-tryptophan and operator DNA binding to the tryptophan repressor of Escherichia coli were analyzed by titration microcalorimetry and van't Hoff analysis of footprinting titrations, respectively. At 25 degrees C in 10 mM sodium phosphate, pH 7.6, and 0.1 M NaCl, the binding of L-tryptophan to the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00079a029
更新日期：1993-07-20 00:00:00
abstract：:Interaction of affinity chromatographically purified antihistone H3 and antihistone H4 with isolated HeLa core particles, followed by separation of unreacted and reacted particles by sedimentation, demonstrates that every core particle contains these histones. Taken together with our previous data indicating the prese...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00643a033
更新日期：1977-11-29 00:00:00
abstract：:We describe the crystal structure of human epidermal-type fatty acid binding protein (E-FABP) that was recently found to be highly upregulated in human psoriatic keratinocytes. To characterize E-FABP with respect to ligand-binding properties and tertiary structure, we cloned the respective cDNA, overexpressed the prot...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi990305u
更新日期：1999-09-21 00:00:00
abstract：:Acetohydroxy acid synthase (AHAS) is a thiamin diphosphate (ThDP)-dependent enzyme that catalyzes the first common step in the biosynthesis of branched-chain amino acids, condensation of pyruvate with a second 2-ketoacid to form either acetolactate or acetohydroxybutyrate. AHAS isozyme II from Escherichia coli is spec...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi102051h
更新日期：2011-04-19 00:00:00
abstract：:The Haemophilus influenzae diaminopimelate epimerase was cloned, expressed, purified, and crystallized in the C2221 space group (a = 102.1 A, b = 115.4 A, c = 66.3 A, alpha = beta = gamma = 90 degrees). The three-dimensional structure was solved to 2.7 A using a single Pt derivative and the Se-Met-substituted enzyme t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi982138o
更新日期：1998-11-24 00:00:00
abstract：:Phospholipase A2 from Naja naja naja venom (Indian cobra) undergoes a concentration-dependent aggregation, and at an assay concentration of 1 microgram mL-1, it exists as a monomer. However, there is some evidence that the enzyme is actually active as a dimer or higher order aggregate. Previous attempts to determine t...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00343a018
更新日期：1985-10-22 00:00:00
abstract：:Beta2-microglobulin (beta2-m) can form dialysis-related amyloid deposits. The structure of a fragment of beta2-m (K3, Ser20-Lys41) in the oligomeric state has recently been solved. We modeled equilibrium structures of K3 oligomers with different organizations (single and double layers) and morphologies (linear-like an...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi7019194
更新日期：2008-02-26 00:00:00
abstract：:An allelic variant of the ouabain-insensitive rat kidney Na+,K(+)-ATPase alpha 1-isoform was identified by chance in a cDNA library. The variant differed from the wild-type rat kidney Na+,K(+)-ATPase by a single G to C base substitution in the cDNA, which on the amino acid level gave rise to a glutamine in place of th...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00211a048
更新日期：1993-12-07 00:00:00
abstract：:To characterize the roles played by Glu44 and Glu56 of cytochrome b5 in the formation of the electrostatic complex between cytochrome c and cytochrome b5, the Glu44, Glu56, or both sites were changed to alanine by site-directed mutagenesis. The influence of these two residues on the protein stability was probed by inv...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi9805036
更新日期：1998-10-06 00:00:00
abstract：:Antimicrobial peptides (AMPs) are important components of the host innate immune system. Papiliocin is a 37-residue AMP purified from larvae of the swallowtail butterfly Papilio xuthus. Magainin 2 is a 23-residue AMP purified from the skin of the African clawed frog Xenopus laevis. We designed an 18-residue hybrid pep...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.5b00392
更新日期：2015-06-30 00:00:00
abstract：:A Rhodobacter sphaeroides bchD (magnesium chelatase) mutant was studied to determine the properties of its photosystem in the absence of bacteriochlorophyll (BChl). Western blots of reaction center H, M, and L (RC H/M/L) proteins from mutant membranes showed levels of 12% RC H, 32% RC L, and 46% RC M relative to those...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi701407k
更新日期：2007-10-30 00:00:00
abstract：:Raman spectra of the parallel-stranded duplex formed from the deoxyoligonucleotides 5'-d-[(A)10TAATTTTAAATATTT]-3' (D1) and 5'-d[(T)10ATTAAAATTTATAAA]-3' (D2) in H2O and D2O have been acquired. The spectra of the parallel-stranded DNA are then compared to the spectra of the antiparallel double helix formed from the de...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00226a012
更新日期：1991-03-26 00:00:00
abstract：:Low-temperature Fourier transform infrared spectroscopy has been used to study mutants of Glu194 and Glu204, two amino acids that are involved in proton release to the extracellular side of bacteriorhodopsin. Difference spectra of films of E194Q, E204Q, E194Q/E204Q, E9Q/E194Q/E204Q, and E9Q/E74Q/E194Q/E204Q at 243, 27...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi025654u
更新日期：2002-06-25 00:00:00
abstract：:High-resolution 2D NMR spectra of the duplex CGCGAATTCGCG with deoxyribose sugars but with the normal phosphodiester linker replaced by an N3' --> P5' phosphoramidate (NP) group have been used to establish a solution structure for the duplex. Distance, angle, and base pair hydrogen-bonding constraints were used to ref...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi980711y
更新日期：1998-09-01 00:00:00
abstract：:We introduced a stop codon in place of the ATT codon encoding Ile283 (numbered from the Met initiation codon) in the petA gene from Chlamydomonas reinhardtii. The resulting protein was expected to be truncated on its carboxy-terminus end, lacking the last 35 amino acids. This region of the polypeptide sequence encompa...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00022a021
更新日期：1995-06-06 00:00:00
abstract：:Studying the evolution of catalytically promiscuous enzymes like those from the N-succinylamino acid racemase/ o-succinylbenzoate synthase (NSAR/OSBS) subfamily can reveal mechanisms by which new functions evolve. Some enzymes in this subfamily have only OSBS activity, while others catalyze OSBS and NSAR reactions. We...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/acs.biochem.8b00088
更新日期：2018-07-03 00:00:00
abstract：:The local environment of the transmembrane and C-terminal domain of M13 major coat protein was probed by site-directed ESR spin labeling when the protein was introduced into three membrane-mimicking systems, DOPC vesicles, sodium cholate micelles, and SDS micelles. For this purpose, we have inserted unique cysteine re...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi961770j
更新日期：1996-12-03 00:00:00
abstract：:The thermotropic behavior and molecular properties of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) and 1-palmitoyl-2-dihydrosterculoyl-sn-glycero-3-phosphoethanolamine (PDSPE) have been investigated by 2H NMR spectroscopy using samples selectively labeled at the 5'-, 9'-, 10'-, and 16'-positions of the...
journal_title：Biochemistry
pub_type： 杂志文章
doi：10.1021/bi00325a038
更新日期：1985-02-12 00:00:00
