Resonance energy transfer between green fluorescent protein variants: complexities revealed with myosin fusion proteins.

Abstract:

:Green fluorescent protein and its variants are frequently used as Förster (fluorescence) resonance energy transfer (FRET) pairs to determine the proximity of protein domains. We prepared fusion proteins comprising yellow fluorescent protein-Dictyostelium myosin II motor domain-cyan fluorescent protein (YFP-myosin-CFP) and compared their FRET properties with an existing construct (GFP-myosin-BFP), containing a green fluorescent protein acceptor and blue fluorescent protein donor [Suzuki, Y., Yasunaga, T., Ohkura, R., Wakabayashi, T. and Sutoh, K. (1998) Nature 396, 380-383]. The latter construct showed an apparent 40% reduction in acceptor fluorescence on ATP addition, when excited via the donor, compared with the YFP-myosin-CFP constructs which showed a small increase (

journal_name

Biochemistry

journal_title

Biochemistry

authors

Zeng W,Seward HE,Málnási-Csizmadia A,Wakelin S,Woolley RJ,Cheema GS,Basran J,Patel TR,Rowe AJ,Bagshaw CR

doi

10.1021/bi060943u

subject

Has Abstract

pub_date

2006-09-05 00:00:00

pages

10482-91

issue

35

eissn

0006-2960

issn

1520-4995

journal_volume

45

pub_type

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