Identification of contact sites between ankyrin and band 3 in the human erythrocyte membrane.


:The red cell membrane is stabilized by a spectrin/actin-based cortical cytoskeleton connected to the phospholipid bilayer via multiple protein bridges. By virtue of its interaction with ankyrin and adducin, the anion transporter, band 3 (AE1), contributes prominently to these bridges. In a previous study, we demonstrated that an exposed loop comprising residues 175-185 of the cytoplasmic domain of band 3 (cdB3) constitutes a critical docking site for ankyrin on band 3. In this paper, we demonstrate that an adjacent loop, comprising residues 63-73 of cdB3, is also essential for ankyrin binding. Data that support this hypothesis include the following. (1) Deletion or mutation of residues within the latter loop abrogates ankyrin binding without affecting cdB3 structure or its other functions. (2) Association of cdB3 with ankyrin is inhibited by competition with the loop peptide. (3) Resealing of the loop peptide into erythrocyte ghosts alters membrane morphology and stability. To characterize cdB3-ankyrin interaction further, we identified their interfacial contact sites using molecular docking software and the crystal structures of D(3)D(4)-ankyrin and cdB3. The best fit for the interaction reveals multiple salt bridges and hydrophobic contacts between the two proteins. The most important ion pair interactions are (i) cdB3 K69-ankyrin E645, (ii) cdB3 E72-ankyrin K611, and (iii) cdB3 D183-ankyrin N601 and Q634. Mutation of these four residues on ankyrin yielded an ankyrin with a native CD spectrum but little or no affinity for cdB3. These data define the docking interface between cdB3 and ankyrin in greater detail.






Grey JL,Kodippili GC,Simon K,Low PS




Has Abstract


2012-08-28 00:00:00












  • 19F-NMR spin-spin relaxation (T2) method for characterizing volatile anesthetic binding to proteins. Analysis of isoflurane binding to serum albumin.

    abstract::This paper characterizes the low-affinity ligand binding interactions of a fluorinated volatile anesthetic, isoflurane (CHF2OCHClCF3), with bovine serum albumin (BSA) using 19F-NMR transverse relaxation (T2). 19F-NMR spectra of isoflurane in aqueous BSA reveal a single isoflurane trifluoromethyl resonance, indicative ...


    pub_type: 杂志文章


    authors: Dubois BW,Evers AS

    更新日期:1992-08-11 00:00:00

  • Role of 2',5'-oligo(adenylic acid) polymerase in the degradation of ribonucleic acid linked to double-stranded ribonucleic acid by extracts of interferon-treated cells.

    abstract::RNA covalently linked to double-stranded RNA (dsRNA) is preferentially degraded in extracts of interferon-treated HeLa cells [Nilsen, T. W., & Baglioni, C. (1979) Proc. Natl. Acad. Sci. U.S.A. 76, 2600-2604]. The size of the dsRNA required for this preferential degradation has been determined by annealing poly(I) of k...


    pub_type: 杂志文章


    authors: Nilsen TW,Weissman SG,Baglioni C

    更新日期:1980-11-25 00:00:00

  • Long-range structural and dynamical changes induced by cofactor binding in DNA methyltransferase M.HhaI.

    abstract::The bacterial DNA cytosine methyltransferase M.HhaI sequence-specifically modifies DNA in an S-adenosylmethionine dependent reaction. The enzyme stabilizes the target cytosine (GCGC) into an extrahelical position, with a concomitant large movement of an active site loop involving residues 80-99. We used multidimension...


    pub_type: 杂志文章


    authors: Zhou H,Shatz W,Purdy MM,Fera N,Dahlquist FW,Reich NO

    更新日期:2007-06-19 00:00:00

  • Purification and characterization of the messenger ribonucleic acid capping enzyme GTP:RNA guanylyltransferase from wheat germ.

    abstract::A GTP:RNA guanylyltransferase or capping enzyme has been purified approximately 2000-fold from wheat germ. The enzyme catalyzes the transfer of the GMP residue from GTP to the 5' end of RNA or synthetic polyribonucleotides. Diphosphate-ended polymers were capped more efficiently than molecules with triphosphate ends, ...


    pub_type: 杂志文章


    authors: Keith JM,Venkatesan S,Gershowitz A,Moss B

    更新日期:1982-01-19 00:00:00

  • Avian synaptopodin 2 (fesselin) stabilizes myosin filaments and actomyosin in the presence of ATP.

    abstract::Smooth muscle cells maintain filaments of actin and myosin in the presence of ATP, although dephosphorylated myosin filaments and actin-myosin interactions are unstable under those conditions in vitro. Several proteins that stabilize myosin filaments and that stabilize actin-myosin interactions have been identified. F...


    pub_type: 杂志文章


    authors: Kingsbury NL,Renegar RH,Chalovich JM

    更新日期:2013-10-29 00:00:00

  • Zn(2+) ions selectively induce antimicrobial salivary peptide histatin-5 to fuse negatively charged vesicles. Identification and characterization of a zinc-binding motif present in the functional domain.

    abstract::The salivary antimicrobial peptide histatin-5 is able to aggregate and fuse negatively charged small unilamellar vesicles, and this fusogenic activity is selectively induced by the presence of zinc ions. Circular dichroism spectroscopy shows that histatin-5, in the presence of negatively charged vesicles and zinc ions...


    pub_type: 杂志文章


    authors: Melino S,Rufini S,Sette M,Morero R,Grottesi A,Paci M,Petruzzelli R

    更新日期:1999-07-27 00:00:00

  • Reactivity of horse liver alcohol dehydrogenase with 3-methylcyclohexanols.

    abstract::The specificity of horse liver alcohol dehydrogenase for cyclohexanol and its 3-methyl derivatives was investigated by stopped-flow and initial velocity kinetic studies. The (1S,3S)-3-methylcyclohexanol was 7 times more reactive (V/Km) than cyclohexanol, whereas the (1R,3R)-3-methylcyclohexanol was at least 1000 times...


    pub_type: 杂志文章


    authors: Lee KM,Dahlhauser KF,Plapp BV

    更新日期:1988-05-03 00:00:00

  • Interaction of the fluorescence-labeled secretory component with human polymeric immunoglobulins.

    abstract::The secretory component (SC) isolated from human milk was labeled with 2 mol of the fluorescent thiol reagent N-[7-(dimethylamino)-4-methylcoumarinyl]maleimide (DACM) per mol of SC through the reactive disulfide bond of SC. The binding of the labeled SC to polymeric immunoglobulins was examined by gel filtration by me...


    pub_type: 杂志文章


    authors: Goto Y,Aki K

    更新日期:1984-12-18 00:00:00

  • Secondary electron transfer processes in membranes of Heliobacillus mobilis.

    abstract::Picosecond transient absorption difference spectroscopic experiments were performed on membranes of the antenna/reaction center complex of Heliobacillus mobilis to study the electron transfer processes. Particular emphasis was placed on the blue spectral region, where the difference spectra of iron-sulfur centers and ...


    pub_type: 杂志文章


    authors: Lin S,Chiou HC,Blankenship RE

    更新日期:1995-10-03 00:00:00

  • 3-Bromotyrosine and 3,5-dibromotyrosine are major products of protein oxidation by eosinophil peroxidase: potential markers for eosinophil-dependent tissue injury in vivo.

    abstract::Detection of specific reaction products is a powerful approach for dissecting out pathways that mediate oxidative damage in vivo. Eosinophil peroxidase (EPO), an abundant protein secreted from activated eosinophils, has been implicated in promoting oxidative tissue injury in conditions such as asthma, allergic inflamm...


    pub_type: 杂志文章


    authors: Wu W,Chen Y,d'Avignon A,Hazen SL

    更新日期:1999-03-23 00:00:00

  • Ion concentration and temperature dependence of DNA binding: comparison of PurR and LacI repressor proteins.

    abstract::Purine repressor (PurR) binding to specific DNA is enhanced by complexing with purines, whereas lactose repressor (LacI) binding is diminished by interaction with inducer sugars despite 30% identity in their protein sequences and highly homologous tertiary structures. Nonetheless, in switching from low- to high-affini...


    pub_type: 杂志文章


    authors: Moraitis MI,Xu H,Matthews KS

    更新日期:2001-07-10 00:00:00

  • Understanding functional divergence in proteins by studying intragenomic homologues.

    abstract::Studies of intragenomic homologues in bacterial genomes can provide valuable insights into functional divergence. Three GTP cyclohydrolase II homologues in the Streptomyces coelicolor genome have been shown to catalyze two related but distinct reactions [Spoonamore, J. E., Dahlgran, A. L., Jacobsen, N. E., and Bandari...


    pub_type: 杂志文章


    authors: Spoonamore JE,Bandarian V

    更新日期:2008-02-26 00:00:00

  • Synthesis and properties of fluorescent beta-adrenoceptor ligands.

    abstract::We describe the synthesis of bordifluoropyrromethene (BODIPY), fluorescein, and related fluorescent derivatives of the beta-adrenergic ligand CGP 12177. With these probes we screened insect (Sf9) cells stably transformed with the human beta 2-adrenoceptor gene and expressing (2-3.5) x 10(5) human beta 2-adrenoceptors ...


    pub_type: 杂志文章


    authors: Heithier H,Hallmann D,Boege F,Reiländer H,Dees C,Jaeggi KA,Arndt-Jovin D,Jovin TM,Helmreich EJ

    更新日期:1994-08-09 00:00:00

  • The transcription regulator RcoM-2 from Burkholderia xenovorans is a cysteine-ligated hemoprotein that undergoes a redox-mediated ligand switch.

    abstract::Spectroscopic characterization of the newly discovered heme-PAS domain sensor protein BxRcoM-2 reveals that this protein undergoes redox-dependent ligand switching and CO- and NO-induced ligand displacement. The aerobic bacterium Burkholderia xenovorans expresses two homologous heme-containing proteins that promote CO...


    pub_type: 杂志文章


    authors: Marvin KA,Kerby RL,Youn H,Roberts GP,Burstyn JN

    更新日期:2008-08-26 00:00:00

  • Complete amino acid sequence of the 4Fe-4S, thermostable ferredoxin from Clostridium thermoaceticum.

    abstract::The complete amino acid sequence of the 4Fe-4S ferredoxin from the thermophilic bacterium Clostridium thermoaceticum has been determined. The protein is extremely thermostable and is the only known clostridial ferredoxin to contain a single [4Fe-4S] cluster. The sequence totals 63 residues and includes the first trypt...


    pub_type: 杂志文章


    authors: Elliott JI,Yang SS,Ljungdahl LG,Travis J,Reilly CF

    更新日期:1982-07-06 00:00:00

  • Deamidation of Human γS-Crystallin Increases Attractive Protein Interactions: Implications for Cataract.

    abstract::Deamidation of proteins is one of the most prevalent post-translational modifications found upon aging, and in age-onset diseases. Specific asparagine and glutamine residues are often selectively deamidated during this process. In the human lens, deamidation has been shown to occur in many crystallins, but it is not c...


    pub_type: 杂志文章


    authors: Pande A,Mokhor N,Pande J

    更新日期:2015-08-11 00:00:00

  • Thermodynamics of the dimer-decamer transition of reduced human and plant 2-cys peroxiredoxin.

    abstract::Isothermal titration calorimetry (ITC) is a powerful technique for investigating self-association processes of protein complexes and was expected to reveal quantitative data on peroxiredoxin oligomerization by directly measuring the thermodynamic parameters of dimer-dimer interaction. Recombinant classical 2-cysteine ...


    pub_type: 杂志文章


    authors: Barranco-Medina S,Kakorin S,Lázaro JJ,Dietz KJ

    更新日期:2008-07-08 00:00:00

  • Partially folded forms of barley lipid transfer protein are more surface active.

    abstract::Thermal and chemical modification of protein structures is known to affect their interfacial activity. We have looked in detail at the effect of heating on the structure and subsequently the surface properties of LTP1, a nonspecific lipid transfer protein from barley, both in the presence and in the absence of its lip...


    pub_type: 杂志文章


    authors: Clare Mills EN,Gao C,Wilde PJ,Rigby NM,Wijesinha-Bettoni R,Johnson VE,Smith LJ,Mackie AR

    更新日期:2009-12-29 00:00:00

  • Accumulation of long-chain acylcarnitine and 3-hydroxy acylcarnitine molecular species in diabetic myocardium: identification of alterations in mitochondrial fatty acid processing in diabetic myocardium by shotgun lipidomics.

    abstract::Diabetic cardiomyopathy is the result of maladaptive changes in energy homeostasis. However, the biochemical mechanisms underlying dysfunctional lipid metabolism in diabetic myocardium are incompletely understood. Herein, we exploit shotgun lipidomics to demonstrate a 4-fold increase in acylcarnitines in diabetic myoc...


    pub_type: 杂志文章


    authors: Su X,Han X,Mancuso DJ,Abendschein DR,Gross RW

    更新日期:2005-04-05 00:00:00

  • Conformational Dynamics of Specific Aβ Oligomers Govern Their Ability To Replicate and Induce Neuronal Apoptosis.

    abstract::Oligomers of amyloid-β (Aβ) have emerged as the primary toxic agents responsible for early synaptic dysfunction and neuronal death in Alzheimer's disease (AD). Characterization of oligomers is an important step in the progress toward delineating the complex molecular mechanisms involved in AD pathogenesis. In our prev...


    pub_type: 杂志文章


    authors: Dean DN,Pate KM,Moss MA,Rangachari V

    更新日期:2016-04-19 00:00:00

  • Identification of essential tyrosine and lysine residues in angiotensin converting enzyme: evidence for a single active site.

    abstract::Inactivation of rabbit lung angiotensin converting enzyme (ACE) by 1-fluoro-2,4-dinitrobenzene (Dnp-F) has been shown to be due primarily to the modification of a tyrosine residue [Bünning, P., Kleeman, S.G., & Riordan, J.F. (1990) Biochemistry (preceding paper in this issue)]. Rabbit testicular ACE is also inactivate...


    pub_type: 杂志文章


    authors: Chen YN,Riordan JF

    更新日期:1990-11-20 00:00:00

  • A conformational mimic of the MgATP-bound "on state" of the nitrogenase iron protein.

    abstract::The crystal structure of a nitrogenase Fe protein single site deletion variant reveals a distinctly new conformation of the Fe protein and indicates that, upon binding of MgATP, the Fe protein undergoes a dramatic conformational change that is largely manifested in the rigid-body reorientation of the homodimeric Fe pr...


    pub_type: 杂志文章


    authors: Sen S,Igarashi R,Smith A,Johnson MK,Seefeldt LC,Peters JW

    更新日期:2004-02-24 00:00:00

  • Mutations affecting transition-state stabilization by residues coordinating zinc at the active site of cytidine deaminase.

    abstract::Cytidine deaminase from Escherichia coli contains 1 mol of tightly bound zinc per enzyme subunit (Yang, C., Carlow, D., Wolfenden, R., & Short, S.A. (1992) Biochemistry 31, 4168-4174). When the metal liganding residues Cys-129 and Cys-132 were replaced by Ala, and His-102 was replaced by Ala, Asn, or Gln, deaminase ac...


    pub_type: 杂志文章


    authors: Smith AA,Carlow DC,Wolfenden R,Short SA

    更新日期:1994-05-31 00:00:00

  • Ligand-free open-closed transitions of periplasmic binding proteins: the case of glutamine-binding protein.

    abstract::The ability to undergo large-scale domain rearrangements is essential for the substrate-binding function of periplasmic binding proteins (PBPs), which are indispensable for nutrient uptake in Gram-negative bacteria. Crystal structures indicate that PBPs typically adopt either an "open" unliganded configuration or a "c...


    pub_type: 杂志文章


    authors: Bermejo GA,Strub MP,Ho C,Tjandra N

    更新日期:2010-03-09 00:00:00

  • The 1.6 A crystal structure of Mycobacterium smegmatis MshC: the penultimate enzyme in the mycothiol biosynthetic pathway.

    abstract::Mycobacterium smegmatis MshC catalyzes the ATP-dependent condensation of GlcN-Ins and l-cysteine to form l-Cys-GlcN-Ins, the penultimate step in mycothiol biosynthesis. Attempts to crystallize the native, full-length MshC have been unsuccessful. However, incubation of the enzyme with the cysteinyl adenylate analogue, ...


    pub_type: 杂志文章


    authors: Tremblay LW,Fan F,Vetting MW,Blanchard JS

    更新日期:2008-12-16 00:00:00

  • Stabilities of nucleotide loops bridging the pyrimidine strands in DNA pyrimidine.purine.pyrimidine triplexes: special stability of the CTTTG loop.

    abstract::Recent studies of DNA hairpin loops have shown considerable dependence of the stability on the sequence of the loop [Senior, M., Jones, R. A., & Breslauer, K. J. (1988a) Proc. Natl. Acad. Sci. U.S.A. 85, 6242-6246; Xodo, L. E., Manzini, G., Quadrifoglio, F., van der Marel, G., & van Boom, J. H. (1989) Biochimie 71, 79...


    pub_type: 杂志文章


    authors: Wang S,Booher MA,Kool ET

    更新日期:1994-04-19 00:00:00

  • Extreme Dynamics in the BamA β-Barrel Seam.

    abstract::BamA is an essential component of the β-barrel assembly machine (BAM) that is responsible for insertion and folding of β-barrel outer membrane proteins (OMPs) in Gram-negative bacteria. BamA is an OMP itself, and its β-barrel transmembrane domain is thought to catalyze OMP insertion and folding, although the molecular...


    pub_type: 杂志文章


    authors: Doerner PA,Sousa MC

    更新日期:2017-06-20 00:00:00

  • Solution structure of the N-terminal EGF-like domain from human factor VII.

    abstract::Blood coagulation is initiated by Ca(2+)-dependent binding of coagulation factor VIIa (FVIIa) to its cofactor, tissue factor (TF). The TF:FVIIa complex activates factors IX and X, ultimately leading to the formation of thrombin and the coagulation of blood. FVII consists of an N-terminal gamma-carboxyglutamic-acid-con...


    pub_type: 杂志文章


    authors: Muranyi A,Finn BE,Gippert GP,Forsén S,Stenflo J,Drakenberg T

    更新日期:1998-07-28 00:00:00

  • Assembly of microtubules from tubulin bearing the nonhydrolyzable guanosine triphosphate analogue GMPPCP [guanylyl 5'-(beta, gamma-methylenediphosphonate)]: variability of growth rates and the hydrolysis of GTP.

    abstract::The growth and shortening of microtubules in guanosine triphosphate-(GTP-) mediated dynamic instability has previously been observed to occur at rates which are remarkably variable (Gildersleeve et al., 1992, Chrétien et al., 1995). Neighboring microtubules observed simultaneously can grow or shorten at different rate...


    pub_type: 杂志文章


    authors: Dye RB,Williams RC Jr

    更新日期:1996-11-12 00:00:00

  • Effect of structural modifications on the activity of the leadzyme.

    abstract::The structure/function properties of functional groups in the leadzyme have been studied by assaying the activity of analog ribozymes generated by the systematic substitution of modified nucleotides in the internal loop region of the ribozyme. Guanosine analogs introduced at positions 4 and 7 occupied by guanosine in ...


    pub_type: 杂志文章


    authors: Chartrand P,Usman N,Cedergren R

    更新日期:1997-03-18 00:00:00