Abstract:
:Typical signs of cardiomyoblasts were determined: high mitochondrial membrane potential and high number of mitochondria in these cells compared to fibroblasts. These signs can be used for identification of these cells. Energy-dependent accumulation of highly specific mitochondrial fluorescent probes applied for visual detection of energized mitochondria allows clear-cut separation of the mixed population: cardiomyocyte population is characterized by higher transmembrane potential than concomitant cells. Using flow cytometry and cell sorting we obtained a population enriched with cardiomyocytes and cardiomyoblasts. Taking into account the fact that the dye has no toxic effect on cells and is rapidly eliminated, the risk of cell damage and death during isolation is considerably reduced compared to traditional methods. These results open possibilities for the development of a new specific method for isolation of cardiomyocyte culture from fetal and embryonic sources for their further use in clinical practice.
journal_name
Bull Exp Biol Medjournal_title
Bulletin of experimental biology and medicineauthors
Khryapenkova TG,Plotnikov EY,Korotetskaya MV,Sukhikh GT,Zorov DBdoi
10.1007/s10517-009-0327-3subject
Has Abstractpub_date
2008-10-01 00:00:00pages
506-11issue
4eissn
0007-4888issn
1573-8221journal_volume
146pub_type
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