Abstract:
:The immunolocalization of apoptotic factors in rat neocortex was studied on the model of permanent occlusion of the middle cerebral artery with administration of exogenous BDNF. We revealed heterogeneous distribution of pro- and anti-apoptotic factors in the stroke area and in the surrounding penumbra, where caspase-3+ and p53+ cells were found. Their number was maximum on day 3 of ischemia. The number of neurons containing anti-apoptotic factors was relatively decreased. Injection of BDNF changed the distribution of apoptotic factors. In the penumbra area, BDNF enhanced the expression of Mdm2 primarily in the pyramid cells of layers V/VI and Bcl-2 in interneurons of layers II and III. Localization of p53 and caspase-3 varied at different terms of the ischemic period and showed an inverse dependence. Considering the selective neuroprotective effect of BDNF, various mechanisms of the formation of ischemic tolerance in neurons are proposed.
journal_name
Bull Exp Biol Medjournal_title
Bulletin of experimental biology and medicineauthors
Kalinichenko SG,Matveeva NY,Korobtsov AVdoi
10.1007/s10517-020-04959-7subject
Has Abstractpub_date
2020-09-01 00:00:00pages
701-706issue
5eissn
0007-4888issn
1573-8221pii
10.1007/s10517-020-04959-7journal_volume
169pub_type
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