Abstract:
:We aim to develop a method that confers rapid protection against foot-and-mouth disease (FMD) by passive immunization with recombinant llama single-domain antibody fragments (VHHs). Previously constructed genetic fusions of two VHHs (VHH2s) that either neutralizes FMDV or binds to porcine immunoglobulin to increase the serum half-life, conferred only limited protection to pigs. We therefore now generated VHH3s containing an additional FMDV binding VHH. Two VHH3s neutralized FMDV more potently than single VHHs and were highly produced by yeast cells. Injection of a mixture of these two VHH3s 24h before FMD challenge infection of pigs reduced and delayed the development of clinical disease, viraemia and viral shedding. Furthermore, it significantly (P=0.023) delayed FMD transmission. Thus, we have shown a proof of concept of passive FMD immunoprophylaxis using VHHs.
journal_name
Vaccinejournal_title
Vaccineauthors
Harmsen MM,Fijten HP,Engel B,Dekker A,Eblé PLdoi
10.1016/j.vaccine.2009.01.110subject
Has Abstractpub_date
2009-03-18 00:00:00pages
1904-11issue
13eissn
0264-410Xissn
1873-2518pii
S0264-410X(09)00173-Xjournal_volume
27pub_type
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