Abstract：
:The purpose of this study was to investigate the utility of various in vitro and in vivo methods to assess the stability of experimental vaccines containing tetanus toxoid (TT) within PLGA microspheres. In vitro, the breakdown of the encapsulating polymers into their acid components led to changes in the structure of TT, as determined by the physico-chemical methods, rendering it undetectable by capture ELISA and altering its structural integrity. The changes in TT were directly related to increasing acidity of the vaccine supernate. Purified toxoid (not encapsulated) exposed to low pH (2.5) underwent similar changes but re-neutralisation of buffer containing free toxoid, even after one week at pH 2.5 led to some re-folding of protein as determined by fluorescence spectroscopy and gel filtration chromatography. The microencapsulated vaccines were still able to generate an antibody response in mice even after prolonged pre-incubation at 37 degrees C and the apparent absence of detectable toxoid in the vaccine supernate. Electron microscopy demonstrated differences in the amount of degradation between different formulations of microspheres. Vaccines that had retained their spherical morphology after incubation in vitro for up to 28 days were able to induce protective antibodies response equal to that of freshly prepared vaccines, which indicates that the toxoid within intact microspheres remained immunogenic. Immunochemical and physico-chemical detection methods, performed on antigen released from PLGA vaccines in vitro, are valuable in providing information on product characteristics but may not be able to predict effectiveness and should be used with in vivo methods to evaluate the stability of such formulations.
journal_name
Vaccinejournal_title
Vaccineauthors
Sasiak AB,Bolgiano B,Crane DT,Hockley DJ,Corbel MJ,Sesardic Ddoi
10.1016/s0264-410x(00)00266-8subject
Has Abstractpub_date
2000-11-22 00:00:00pages
694-705issue
7-8eissn
0264-410Xissn
1873-2518pii
S0264-410X(00)00266-8journal_volume
19pub_type
杂志文章相关文献
VACCINE文献大全abstract：OBJECTIVE:Rotavirus is the most common cause of severe diarrhea in children. Two rotavirus vaccines (RotaTeq and Rotarix) have been licensed in Taiwan. We have investigated whether routine infant immunization with either vaccine could be cost-effective in Taiwan. METHODS:We modeled specific disease outcomes including ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2009.01.023
更新日期：2009-03-04 00:00:00
abstract：OBJECTIVES:We aimed to estimate vaccine effectiveness against severe measles based on the number of vaccine doses administered and the time since last vaccination. PATIENTS AND METHODS:We included measles cases aged at least 2 years and born since 1980 who were notified in France between 2006 and mid-2019. We consider...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2020.05.005
更新日期：2020-06-15 00:00:00
abstract：BACKGROUND:This study evaluated the immunogenicity and safety of quadrivalent meningococcal conjugate vaccine using tetanus (T) toxoid as carrier protein (MenACWY-TT) co-administered with combined diphtheria-tetanus-acellular pertussis vaccine (Tdap) versus their separate administration in adolescents and young adults....
journal_title：Vaccine
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.vaccine.2018.04.034
更新日期：2018-07-25 00:00:00
abstract：:We demonstrated earlier that immunization with recombinant Leptospira immunoglobulin like protein A (LigA) induced significant protection against virulent Leptospira interrogans serovar Pomona challenge in hamsters. However, the protective immune mechanism remains unclear. In the present study we demonstrated the prot...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2007.10.029
更新日期：2008-01-10 00:00:00
abstract：:Ricin is a plant toxin that is a CDC level B biothreat. Our recombinant ricin A chain vaccine (RiVax), which contains mutations in both known toxic sites, has no residual toxicity at doses at least 800 times the immunogenic dose. RiVax without adjuvant given intramuscularly (i.m.) protected mice against intraperitonea...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2007.08.018
更新日期：2007-10-16 00:00:00
abstract：:In order to evaluate the long-term immunity 2 and 3 years after booster vaccination against tick-borne encephalitis (TBE) following time intervals 3 years and longer since last TBE immunization, 195 (mean age 50.5+/-15.1 years) and 240 subjects (mean age 47.0+/-15.4 years), respectively, who had received a single boos...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2007.01.116
更新日期：2007-06-28 00:00:00
abstract：BACKGROUND:The compliance with influenza vaccination among health-care workers (HCWs) is known to be low. A multi-nationality survey to explore the reasons for such poor compliance has not been studied in depth. MATERIALS AND METHODS:An epidemiologic survey to evaluate the compliance rates with influenza vaccination a...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2009.06.108
更新日期：2009-09-04 00:00:00
abstract：:To evaluate the utility of plasmid DNA vaccination against disease caused by herpes simplex virus (HSV), we compared the strength of protection against lethal challenge following natural virus infection with that following vaccination with a plasmid encoding HSV glycoprotein gD (gD-DNA). We further determined the cell...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(00)00380-7
更新日期：2001-01-08 00:00:00
abstract：:We performed a genome-wide association study (GWAS) of antibody levels in a multi-ethnic group of 1071 healthy smallpox vaccine recipients. In Caucasians, the most prominent association was found with promoter SNP rs10489759 in the LOC647132 pseudogene on chromosome 1 (p=7.77×10(-8)). In African-Americans, we identifi...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2012.04.055
更新日期：2012-06-13 00:00:00
abstract：:Mucin1 (MUC1) is expressed ubiquitously on breast cancer cells and is a potential target for the generation of cytotoxic T cells for vaccination against breast cancer. Thus far studies of the immunogenicity of MUC1 have used peptides from the variable number of tandem repeat (VNTR); mice so immunised can generate stro...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(99)00515-0
更新日期：2000-04-03 00:00:00
abstract：:Early studies using DNA immunization suggest the potential benefits of this form of immunization including: long-lived immunity, a broad spectrum of immune responses (both cell-mediated immunity and humoral responses) and the simultaneous induction of immunity to a variety of pathogens through the use of multivalent v...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(00)00476-x
更新日期：2001-03-21 00:00:00
abstract：:Potential vaccine strategies against otitis media are to prevent (1) symptomatic infections in the middle ear and/or (2) carriage of pneumococci and thereby subsequent middle ear infections. The possibility of using immunity to virulence proteins of pneumococci to elicit immunity against pneumococci has been examined....
journal_title：Vaccine
pub_type： 杂志文章,评审
doi：10.1016/s0264-410x(00)00285-1
更新日期：2000-12-08 00:00:00
abstract：:The burden of influenza in children is increasingly appreciated; some middle-income countries are considering support for influenza vaccine programs. To support decision-making, methods to estimate the potential impact of proposed programs are needed. Using Thailand as a case-study, we present a model that uses survei...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2011.03.099
更新日期：2011-06-10 00:00:00
abstract：:Recombinant replication-defective human adenovirus type 5 (Ad5) vaccines containing capsid-coding regions from foot-and-mouth disease virus (FMDV) have been demonstrated to induce effective immune responses and provide homologous protective immunity against FMDV in cattle. However, basic mechanisms of Ad5-FMDV vaccine...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2011.12.082
更新日期：2012-02-21 00:00:00
abstract：:Respiratory syncytial virus F-protein (RSV-F) is poorly expressed from DNA expression plasmids containing the wild type RSV-F open reading frame. By codon optimization, premature polyadenylation signals were deleted and a striking enhancement of RSV-F expression levels was achieved. Therefore, the immunogenicity and e...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2007.07.025
更新日期：2007-10-10 00:00:00
abstract：:Nasal administration of vaccines is an attractive approach which offers several significant advantages over traditional intramuscular vaccine delivery. These advantages include easier administration and induction of immune responses in the mucosal secretions of the body. In this study we describe a new potent nasal ad...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(01)00156-6
更新日期：2001-07-20 00:00:00
abstract：:The HA1 domain of influenza A viruses is critical for immune escape and host receptor recognition. However, the association between HA1 evolution and host species-specific adaptations remains unclear. We have identified the amino acid sites that are related to the host species-specific adaptations by systematically co...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2010.09.071
更新日期：2010-11-23 00:00:00
abstract：:Mucosal immunization with a killed whole-cell pneumococcal vaccine, given with enterotoxin-related adjuvants, has been shown to confer multi-serotype protection against colonization of the nasopharynx and middle ear in mice. However, because novel mucosal immunization strategies may be difficult to implement, here we ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2010.09.031
更新日期：2010-11-03 00:00:00
abstract：:In response to the resurgence of mumps, the Korean Armed Forces started the measles-mumps-rubella (MMR) vaccination to all new recruits regardless of prior vaccination history. We evaluated the effectiveness of the vaccination by comparing the incidence between the military and civilian populations before and after im...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2017.06.025
更新日期：2017-07-13 00:00:00
abstract：:Sequential or co-administration of vaccines has potential to alter the immune response to any of the antigens. Existing literature suggests that prior immunisation of tetanus/diphtheria-containing vaccines can either enhance or suppress immune response to conjugate pneumococcal or meningococcal vaccines. We examined t...
journal_title：Vaccine
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/j.vaccine.2016.10.020
更新日期：2016-11-21 00:00:00
abstract：:We performed a randomized, controlled clinical trial to characterize the evolution of the adverse events associated with the fifth consecutive dose of an acellular pertussis vaccine, and to assess the level of discomfort associated with the injection and the attitude of parents concerning these events. A total of 505 ...
journal_title：Vaccine
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：10.1016/s0264-410x(03)00173-7
更新日期：2003-06-02 00:00:00
abstract：:A survey of aged veterans greater than or equal to 85 years old was undertaken to determine their vaccination behaviour prior to mounting a local education campaign on influenza vaccine. A questionnaire inquiring into circumstances which may have influenced their decision to be immunized against influenza was mailed t...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/0264-410x(89)90163-1
更新日期：1989-10-01 00:00:00
abstract：:HIV-1 Tat protein activates resting cells, rendering them permissive for viral replication. Replication of HIV-1 in vitro is enhanced by intercellular passage of Tat protein and inhibited by anti-Tat antibodies. Tat dependence of HIV-1 replication in vivo during acute, chronic asymptomatic and AIDS stages of infection...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(00)00085-2
更新日期：2000-06-15 00:00:00
abstract：:The hepatitis E virus (HEV) vaccine, Hecolin(®), was licensed in China for the prevention of HEV infection and HEV-related diseases with demonstrated safety and efficacy [1,2]. The vaccine is composed of a truncated HEV capsid protein, p239, as the sole antigen encoded by open reading frame 2 and produced using Escher...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2014.05.064
更新日期：2014-07-07 00:00:00
abstract：:In line with WHO objectives, the Lao Government is committed to eliminate measles by 2012. Yet from 1992 to 2007, the annual incidence of measles remained high while the vaccination coverage showed a wide diversity across provinces. A descriptive study was performed to determine factors affecting compliance with vacci...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2010.07.077
更新日期：2010-09-24 00:00:00
abstract：:The distribution of alpha-casein, bovine serum albumin (BSA), myoglobin and recombinant protective antigen (rPA) in mono-valent and combination vaccines containing aluminum hydroxide adjuvant was studied by fluorescence microscopy and flow cytometry. Green and red fluorescent probes were conjugated to the antigens. Ad...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2003.10.040
更新日期：2004-05-07 00:00:00
abstract：:Uncertainty regarding the benefits of influenza vaccination may contribute to the underutilization of this vaccine. We have conducted serial cohort studies using the administrative data bases of a Twin Cities based managed care organization to assess the impact of disease and benefits of vaccination among the elderly....
journal_title：Vaccine
pub_type： 杂志文章,多中心研究
doi：10.1016/s0264-410x(99)00105-x
更新日期：1999-07-30 00:00:00
abstract：:EBA-175 protein is used as ligand in Plasmodium falciparum binding to erythrocytes. Evidence shows that conserved peptide 1815 from this protein having high red blood cell binding ability plays an important role in the invasion process. This peptide is neither immunogenic nor protective. Residues were substituted by a...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2004.08.052
更新日期：2005-02-18 00:00:00
abstract：:Reconstituted influenza virus (A/PR/8 strain) envelopes (RIVE) and influenza virus (A/Sichuan/87 (H3N2) strain) surface antigens were entrapped in dehydration-rehydration vesicles (DRV liposomes) composed of egg phosphatidylcholine (PC) or distearoyl phosphatidylcholine (DSPC DRV) and equimolar (32 mumol) cholesterol....
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/0264-410x(92)90509-i
更新日期：1992-01-01 00:00:00
abstract：:To prevent premature dismissal of promising vaccine programs, it is critical to determine if lack of efficacy in the field is due to allele specific-efficacy, rather than to the lack of immunogenicity of the candidate antigen. Here we use samples collected during a field trial of the AMA1-based FMP2.1/AS02A malaria va...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2020.06.035
更新日期：2020-07-31 00:00:00
