Abstract:
:Discrete chromatin domains (ChrD), containing an average of approximately 1 Mbp DNA, represent the basic structural units for the regulation of DNA organization and replication in situ. In this study, a bio-computational approach is employed to simultaneously measure the translational motion of large populations of ChrD in the cell nucleus of living cells. Both movement and configurational changes are strikingly higher in early S-phase replicating ChrD compared to those that replicate in mid and late S-phase. The chromatin dynamics was not sensitive to transcription inhibition by alpha-amanitin but was significantly reduced by actinomycin D treatment. Since a majority of active genes replicate in early S-phase, our results suggest a correlation between levels of chromatin dynamics and chromatin poised for active transcription. Analysis of ChrD colocalization with transcription sites and cDNA with ChrD and transcription sites further supports this proposal.
journal_name
Chromosomajournal_title
Chromosomaauthors
Pliss A,Malyavantham K,Bhattacharya S,Zeitz M,Berezney Rdoi
10.1007/s00412-009-0208-6subject
Has Abstractpub_date
2009-08-01 00:00:00pages
459-70issue
4eissn
0009-5915issn
1432-0886journal_volume
118pub_type
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