Abstract:
:Membrane-free folded chromosomes were purified from log-phase cultures of Escherichia coli and prepared for electron microscopy by aqueous (Kleinschmidt and Zahn) spreading. The appearance of the chromosomes depended on the salt concentrations in spreading. At certain salt concentration, the chromosomes resembled rosettes, with supercoiled loops of DNA radiating from a central core containing RNA. The rosettes support previous models deduced from physical studies of folded chromosomes. Apparently, cores contain must of the visible RNA, and the organization of the core is linked to the organization of the DNA loops.
journal_name
Chromosomajournal_title
Chromosomaauthors
Kavenoff R,Bowen BCdoi
10.1007/BF00328479subject
Has Abstractpub_date
1976-12-16 00:00:00pages
89-101issue
2eissn
0009-5915issn
1432-0886journal_volume
59pub_type
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