Distribution of interstitial telomere-like repeats and their adjacent sequences in a dioecious plant, Silene latifolia.

Abstract:

:The dioecious plant Silene latifolia has large, heteromorphic X and Y sex chromosomes that are thought to be derived from rearrangements of autosomes. To reveal the origin of the sex chromosomes in S. latifolia, we isolated and characterized telomere-homologous sequences from intra-chromosomal regions (interstitial telomere-like repeats; ITRs) and ITR-adjacent sequences (IASs). Nine genomic DNA fragments with degenerate 84- to 175-bp ITRs were isolated from a genomic library and total genome of male plants. Comparing the nucleotide sequences, the IASs of the nine ITRs were classified into seven elements (IAS-a, IAS-b, IAS-c, IAS-d, IAS-e, IAS-f, and IAS-g) by sequence similarity. The ITRs were grouped into two classes (class-I and -II ITRs) according to the classification of IASs. The class-I ITRs were sub-grouped into three subclasses (subclasses-IA, -IB, and -IC ITRs) based on the arrangement of IAS elements. By contrast, the class-II ITR was located between two different IASs (IAS-f and IAS-g). Genomic Southern analyses showed that both the male and female genomes contained six (IAS-f) to 153 (IAS-d) copies of each IAS per haploid genome. Fluorescence in situ hybridization analyses showed that one IAS element, IAS-d, was distributed in the interstitial and proximal regions of the sex chromosomes of S. latifolia. The distribution of IAS-d is important evidence for past telomere-mediated chromosome rearrangements during the evolution of the sex chromosomes of S. latifolia.

journal_name

Chromosoma

journal_title

Chromosoma

authors

Uchida W,Matsunaga S,Sugiyama R,Shibata F,Kazama Y,Miyazawa Y,Hizume M,Kawano S

doi

10.1007/s00412-002-0213-5

subject

Has Abstract

pub_date

2002-12-01 00:00:00

pages

313-20

issue

5

eissn

0009-5915

issn

1432-0886

journal_volume

111

pub_type

杂志文章
  • Spreading synaptonemal complexes from Zea mays. I. No synaptic adjustment of inversion loops during pachytene.

    abstract::Four different inversion heterozygotes of maize were examined for the occurrence of synaptic adjustment. Three substages of pachytene were identified in synaptonemal complex (SC) spreads using side-by-side comparisons of chromosome squashes with two-dimensional spreads of SCs. In SC spreads, inversion loop frequency d...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00286917

    authors: Anderson LK,Stack SM,Sherman JD

    更新日期:1988-01-01 00:00:00

  • Structure of the fission yeast centromere cen3: direct analysis of the reiterated inverted region.

    abstract::We determined the structure of the Schizosaccharomyces pombe centromere cen3 using direct genomic mapping and cosmid walking. The repetitive region of cen3 is approximately 110 kb, much longer than that of the previously determined cen1 and cen2 regions. The approximately 30 kb long left and approximately 60 kb right ...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00365153

    authors: Murakami S,Matsumoto T,Niwa O,Yanagida M

    更新日期:1991-12-01 00:00:00

  • Sex chromosome recombination failure, apoptosis, and fertility in male mice.

    abstract::Lack of crossing-over in meiosis can trigger an apoptotic response at metaphase I by the spindle assembly checkpoint (SAC). In contrast to females, segregation of sex chromosomes in males poses a particular challenge as recombination and chiasma formation is restricted to the pseudoautosomal region, the small region o...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-015-0542-9

    authors: Faisal I,Kauppi L

    更新日期:2016-06-01 00:00:00

  • Localization of tubulin in the mitotic apparatus of mammalian cells by immunofluorescence and immunoelectron microscopy.

    abstract::Antitubulin antibody was used as an immunofluorescent and immunoelectron microscopic probe to localize tubulin in components of the mitotic apparatus of rat kangaroo (strain PtK1) cells in vitro. In addition to the detection of tubulin in the spindle microtubules and centrioles, other structures were found to display ...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00329772

    authors: Pepper DA,Brinkley BR

    更新日期:1977-04-19 00:00:00

  • Heterochromatin markers: a search for heterochromatin specific middle repetitive sequences in Drosophila.

    abstract::We sought for cloned sequences of middle repetitive (MR) complexity that mark obligatory heterochromatic regions. Total genome probes were employed in a differential screening procedure to recover X-specific, Y-specific and autosomal specific heterochromatic sequences. X-and Y-linked sequences were recovered in the sa...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00330119

    authors: Lifschytz E,Hareven D

    更新日期:1982-01-01 00:00:00

  • Immunofluorescent studies of human chromosomes with antibodies against phosphorylated H1 histone.

    abstract::One of the prominent cell cycle-related modifications of histone proteins whose function remains unresolved is the phosphorylation of linker histone H1. In this work we have used indirect immunofluorescence on human cells with antibodies that are specific for phosphorylated histone H1 to examine the cellular distribut...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s004120050400

    authors: Boggs BA,Allis CD,Chinault AC

    更新日期:2000-03-01 00:00:00

  • Nontranscribed spacers in Drosophila ribosomal DNA.

    abstract::Ribosomal DNA nontranscribed spacers in Drosophila virilis DNA have been examined in some detail by restriction site analysis of cloned segments of rDNA, nucleic acid hybridizations involving unfractionated rDNA, and base composition estimates. The overall G + C content of the spacer is 27--28%; this compares with 39%...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00285773

    authors: Rae PM,Barnett T,Murtif VL

    更新日期:1981-01-01 00:00:00

  • Interstrain heterochromatin polymorphisms in Drosophila melanogaster.

    abstract::Neuroblast chromosomes of 16 Drosophila melanogaster laboratory stocks (15 wild type and 1 carrying the mutant vermillion) were carefully analyzed for Q-banding patterns and morphological characteristics, in all the mitotic phases. Two forms of intraspecific heterochromatin variations, involving three types of chromos...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00399131

    authors: Halfer C

    更新日期:1981-01-01 00:00:00

  • A stringent requirement for Plk1 T210 phosphorylation during K-fiber assembly and chromosome congression.

    abstract::Polo-like kinase 1 (Plk1) is an essential mitotic regulator and undergoes periodic phosphorylation on threonine 210, a conserved residue in the kinase's activation loop. While phosphate-mimicking alterations of T210 stimulate Plk1's kinase activity in vitro, their effects on cell cycle regulation in vivo remain contro...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-012-0375-8

    authors: Paschal CR,Maciejowski J,Jallepalli PV

    更新日期:2012-12-01 00:00:00

  • Replication analysis of plasmid DNAs injected into Drosophila embryos.

    abstract::From a "shotgun" collection of DNA fragments, isolated from Drosophila melanogaster, we selected sequences that function as autonomously replicating sequences (ARS) in the yeast Saccharomyces cerevisiae. To investigate the replicative potential of such sequences in Drosophila, five of these ARS elements and also the A...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00344161

    authors: Roth GE

    更新日期:1991-05-01 00:00:00

  • Triplex-forming DNAs in the human interphase nucleus visualized in situ by polypurine/polypyrimidine DNA probes and antitriplex antibodies.

    abstract::The polypurine/polypyrimidine (PuPy) tracts present in the human genome are known to be scattered among and within chromosomes. In PuPy tract sequences, triplex formation occurs readily under physiological conditions, leaving single-stranded DNAs capable of hybridization with complementary single-stranded DNAs and RNA...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-002-0198-0

    authors: Ohno M,Fukagawa T,Lee JS,Ikemura T

    更新日期:2002-09-01 00:00:00

  • A simple and rapid method for constructing ring-X chromosomes in Drosophila melanogaster.

    abstract::Ring chromosomes are of basic interest to the geneticist and cell biologist who study their behavior in meiotic and mitotic divisions. In addition, the mitotic instability associated with some ring-X chromosomes has proven useful in Drosophila as a means to produce gynandromorphs for developmental studies. We describe...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-010-0297-2

    authors: Golic MM,Golic KG

    更新日期:2011-04-01 00:00:00

  • Resolving ambiguities in the karyotype of domestic sheep (Ovis aries). II. G-, Q-, and R-banded idiograms, and chromosome-specific molecular markers.

    abstract::Internally consistent G-, Q- and R-banded karyotypes and idiograms for sheep chromosomes at the 422-band level of resolution are presented. These were derived by sequential Q- to G-staining, and sequential Q- to R-staining of prometaphase spreads prepared from sheep with normal and Robertsonian chromosomes. The fused ...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF02510040

    authors: Ansari HA,Maher DW,Pearce PD,Broad TE

    更新日期:1996-07-01 00:00:00

  • Ki-67: more than a proliferation marker.

    abstract::Ki-67 protein has been widely used as a proliferation marker for human tumor cells for decades. In recent studies, multiple molecular functions of this large protein have become better understood. Ki-67 has roles in both interphase and mitotic cells, and its cellular distribution dramatically changes during cell cycle...

    journal_title:Chromosoma

    pub_type: 杂志文章,评审

    doi:10.1007/s00412-018-0659-8

    authors: Sun X,Kaufman PD

    更新日期:2018-06-01 00:00:00

  • The diameter of chromatin fibres depends on linker length.

    abstract::We have studied the diameter of chromatin fibres embedded in epoxy resins for three different materials: mouse thymus, chicken erythrocytes and sea cucumber spermatozoa. We confirm that the diameter of chromatin fibres increases with linker length, both values being influenced by the protein composition of chromatin. ...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00293338

    authors: Alegre C,Subirana JA

    更新日期:1989-06-01 00:00:00

  • Distinct features of nucleolus-associated domains in mouse embryonic stem cells.

    abstract::Heterochromatin in eukaryotic interphase cells frequently localizes to the nucleolar periphery (nucleolus-associated domains (NADs)) and the nuclear lamina (lamina-associated domains (LADs)). Gene expression in somatic cell NADs is generally low, but NADs have not been characterized in mammalian stem cells. Here, we g...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-020-00734-9

    authors: Bizhanova A,Yan A,Yu J,Zhu LJ,Kaufman PD

    更新日期:2020-06-01 00:00:00

  • The proteins of polytene chromosomes of Drosophila hydei.

    abstract::It is reported that chromatin can be prepared from highly purified polytene nuclei from the salivary glands of third instar larvae of Drosophila hydei; such chromatin differs from that of diploid nuclei mainly by deficiencies in certain nonhistone chromosomal proteins. It is suggested that these proteins are important...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00319831

    authors: Elgin SC,Boyd JB

    更新日期:1975-01-01 00:00:00

  • Heterochromatin, HP1 and methylation at lysine 9 of histone H3 in animals.

    abstract::We show that methylated lysine 9 of histone H3 (Me9H3) is a marker of heterochromatin in divergent animal species. It localises to both constitutive and facultative heterochromatin and replicates late in S-phase of the cell cycle. Significantly, Me9H3 is enriched in the inactive mammalian X chromosome (Xi) in female c...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-002-0182-8

    authors: Cowell IG,Aucott R,Mahadevaiah SK,Burgoyne PS,Huskisson N,Bongiorni S,Prantera G,Fanti L,Pimpinelli S,Wu R,Gilbert DM,Shi W,Fundele R,Morrison H,Jeppesen P,Singh PB

    更新日期:2002-03-01 00:00:00

  • Partitioning the C. elegans genome by nucleosome modification, occupancy, and positioning.

    abstract::We have characterized two post-translational histone modifications in Caenorhabditis elegans on a genomic scale. Micrococcal nuclease digestion and immunoprecipitation were used to obtain distinct populations of single nucleosome cores, which were analyzed using massively parallel DNA sequencing to obtain positional a...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-009-0235-3

    authors: Gu SG,Fire A

    更新日期:2010-02-01 00:00:00

  • Epigenetic changes in mammalian gametes throughout their lifetime: the four seasons metaphor.

    abstract::The ability to reproduce is a major trait of living organisms. This ability is carried out by specialized reproductive cells-gametes. In mammals, gametes develop through a unique developmental pathway. Extensive changes in the epigenome of gametes occur during embryonic development. With birth, gametes continue to mat...

    journal_title:Chromosoma

    pub_type: 杂志文章,评审

    doi:10.1007/s00412-019-00704-w

    authors: Wasserzug-Pash P,Klutstein M

    更新日期:2019-09-01 00:00:00

  • The hBUB1 and hBUBR1 kinases sequentially assemble onto kinetochores during prophase with hBUBR1 concentrating at the kinetochore plates in mitosis.

    abstract::The kinetochore binds an evolutionarily conserved set of checkpoint proteins that function to monitor whether chromosomes have aligned properly at the spindle equator. Human cells contain two related protein kinases, hBUB1 and hBUBR1, that appear to have evolved from a single ancestral BUB1 gene. We generated hBUB1- a...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s004120050322

    authors: Jablonski SA,Chan GK,Cooke CA,Earnshaw WC,Yen TJ

    更新日期:1998-12-01 00:00:00

  • A specific SR protein binds preferentially to the secretory protein gene transcripts in salivary glands of Chironomus tentans.

    abstract::The members of the serine-arginine (SR) family of proteins play multiple roles in posttranscriptional gene expression. Initially considered as essential splicing factors confined to the nucleus and regulating constitutive and alternative splicing, SR proteins are now known to shuttle between the nucleus and the cytopl...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-006-0073-5

    authors: Singh OP,Visa N,Wieslander L,Daneholt B

    更新日期:2006-12-01 00:00:00

  • Developmental changes in midgut chromosomes of Drosophila gibberosa.

    abstract::In Drosophila gibberosa, differences between midgut and salivary gland chromosomes fall into two categories: tissue-specific band modulations which persist throughout the 90 h developmental period that we studied and tissue-specific puffs. Puffs that are common to both tissues tend to appear earlier in the midgut. Som...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00292969

    authors: Roberts PA

    更新日期:1988-11-01 00:00:00

  • Expermental hybridization within the genus Triturus (Urodela: Salamandridae). III. Evidence for crossing-over, true chiasmata and chomosomal homologies in the spermatogenesis of F1 species hybrids, T. cristatus carnifex female X T. marmoratus male.

    abstract::Spermatogenesis in the F1 hybrid (2n=24=12 female + 12 male) between the closely related newt species T. cristatus carnifex and T. marmoratus was apparently normal up to pachytene. Many unpaired chromosomes were present at diplotene and a typical diakinesis was lacking. Primary spermatocytes at meta- and meta-anaphase...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00331572

    authors: Mancino G,Ragghianti M,Bucci-Innocenti S

    更新日期:1979-08-10 00:00:00

  • RNA polymerase II transcription and the functional organization of the mammalian cell nucleus.

    abstract::The study of RNA pol II-mediated transcription regulation has been dominated by molecular biological approaches. Although these methods continue to provide important insights, other approaches are required to insure against an oversimplified view of gene expression. Improvements in EM methods and the development of th...

    journal_title:Chromosoma

    pub_type: 杂志文章,评审

    doi:10.1007/BF00355315

    authors: Hendzel MJ,Bazett-Jones DP

    更新日期:1995-02-01 00:00:00

  • Altered distribution of MLH1 foci is associated with changes in cohesins and chromosome axis compaction in an asynaptic mutant of tomato.

    abstract::In most multicellular eukaryotes, synapsis [synaptonemal complex (SC) formation] between pairs of homologous chromosomes during prophase I of meiosis is closely linked with crossing over. Asynaptic mutants in plants have reduced synapsis and increased univalent frequency, often resulting in genetically unbalanced game...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-012-0363-z

    authors: Qiao H,Offenberg HH,Anderson LK

    更新日期:2012-06-01 00:00:00

  • Changes in DNA topology during spermatogenesis.

    abstract::DNA topology in histone- and protamine-depleted nuclei (nucleoids) from somatic cells, sperm, and spermatogenic cells was studied to determine if the superhelical configuration of DNA looped domains is altered during spermatogenesis. The expansion and contraction of nucleoid DNA was measured with a fluorescence micros...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00288496

    authors: Risley MS,Einheber S,Bumcrot DA

    更新日期:1986-01-01 00:00:00

  • Interactions between BTB domain of CP190 and two adjacent regions in Su(Hw) are required for the insulator complex formation.

    abstract::The best-studied Drosophila insulator complex consists of two BTB-containing proteins, the Mod(mdg4)-67.2 isoform and CP190, which are recruited cooperatively to chromatin through interactions with the DNA-binding architectural protein Su(Hw). While Mod(mdg4)-67.2 interacts only with Su(Hw), CP190 interacts with many ...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/s00412-017-0645-6

    authors: Melnikova L,Kostyuchenko M,Molodina V,Parshikov A,Georgiev P,Golovnin A

    更新日期:2018-03-01 00:00:00

  • Chromosome markers in Mus musculus: differences in C-banding between the subspecies M.m. musculus and M.m. molossinus.

    abstract::Quinacrine (Q-band) and centromeric heterochromatin (C-band) patterns of metaphase chromosomes of two subspecies of Mus musculus were compared. M.m. musculus (the laboratory mouse) and M.m. molossinus (a subspecies from Southeast Asia) had similar Q-band patterns along the length of the chromosomes, but differences we...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00294081

    authors: Dev VG,Miller DA,Tantravahi R,Schreck RR,Roderick TH,Erlanger BF,Miller OJ

    更新日期:1975-12-29 00:00:00

  • Population cytogenetics of the genus Caledia (Orthoptera: Acridinae). I. Inter- and intraspecific karyotype diversity.

    abstract::The genus Caledia acontains two species. C. species nova 1 is restricted to the Oriomo Plateau of S. W. Papua and has a complement of twelve telocentric chromosomes. The second species C. captiva has a much wider distribution pattern--from S.W. Papua in the North, down the entire Eastern seaboard of Australia to South...

    journal_title:Chromosoma

    pub_type: 杂志文章

    doi:10.1007/BF00293452

    authors: Shaw DD

    更新日期:1976-02-23 00:00:00