Abstract：
:Lack of crossing-over in meiosis can trigger an apoptotic response at metaphase I by the spindle assembly checkpoint (SAC). In contrast to females, segregation of sex chromosomes in males poses a particular challenge as recombination and chiasma formation is restricted to the pseudoautosomal region, the small region of homology between X and Y chromosomes. Existing data indicate that low levels of crossover failure in male meiosis can be tolerated without compromising fertility, while high levels of X-Y dissociation (in ≥70 % of cells) result in widespread apoptosis and subsequent infertility, demonstrated earlier, e.g., in Spo11β-only mice. Here, we explore the threshold of X-Y recombination failure frequency that is compatible with fertility. We show that in Spo11β-only(mb) mice with a mixed genetic background, in contrast to Spo11β-only mice with a C57BL/6 background, X-Y pairing fails in ~50 % of cells but this still allows for sperm production without any overt impact on fertility. We also review data on apoptosis and fertility from other achiasmate mouse models and propose that the incidence of homolog dissociation that can be tolerated in vivo without compromising male fertility lies between 50 and 70 %.
journal_name
Chromosomajournal_title
Chromosomaauthors
Faisal I,Kauppi Ldoi
10.1007/s00412-015-0542-9subject
Has Abstractpub_date
2016-06-01 00:00:00pages
227-35issue
2eissn
0009-5915issn
1432-0886pii
10.1007/s00412-015-0542-9journal_volume
125pub_type
杂志文章相关文献
CHROMOSOMA文献大全abstract：:Neuroblast chromosomes of 16 Drosophila melanogaster laboratory stocks (15 wild type and 1 carrying the mutant vermillion) were carefully analyzed for Q-banding patterns and morphological characteristics, in all the mitotic phases. Two forms of intraspecific heterochromatin variations, involving three types of chromos...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00399131
更新日期：1981-01-01 00:00:00
abstract：:The study of RNA pol II-mediated transcription regulation has been dominated by molecular biological approaches. Although these methods continue to provide important insights, other approaches are required to insure against an oversimplified view of gene expression. Improvements in EM methods and the development of th...
journal_title：Chromosoma
pub_type： 杂志文章,评审
doi：10.1007/BF00355315
更新日期：1995-02-01 00:00:00
abstract：:During DNA synthesis there is a distinct stage immediately after the joining of large DNA replication intermediates (post-elongation stage). The conversion of this DNA to mature DNA was analysed in cells treated with aphidicolin to stop the movement of the replication fork. In such cells mature DNA is formed. In contr...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00330347
更新日期：1987-01-01 00:00:00
abstract：:Replication times for all important chromosome bands, of both types R and Q (277 structures) are analysed. The R-bands form a group of structures whose DNA replicates during the early S-phase, while the DNA situated in the Q-bands replicates during the late S-phase. There may not exist overlapping between replication ...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00293440
更新日期：1976-10-12 00:00:00
abstract：:In situ hybridization of labeled DNA of four mobile dispersed genetic elements (mdg), isolated from D. melanogaster and C. virilis genomes, with polytene chromosomes of the larvae of several Drosophila species has been carried out. The data show that the mdg elements exhibit a high degree of species specificity. The s...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00330360
更新日期：1982-01-01 00:00:00
abstract：:With simultaneous immunofluorescence and fluorescent in situ hybridization, we have determined the organization of native and heterologous DNA sequences relative to the cores of meiotic prophase chromosomes. The normal chromatin organization is demonstrated with probes of mouse sequences: a cosmid probe that identifie...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00362284
更新日期：1994-10-01 00:00:00
abstract：:The hypothesis that the genome is composed of a patchwork of structural and functional domains (units) that may be either active or repressed was proposed almost 30 years ago. Here, we examine the evolution of the domain model of eukaryotic genome organization in view of the expansion of genome-scale techniques in the...
journal_title：Chromosoma
pub_type： 杂志文章,评审
doi：10.1007/s00412-016-0604-7
更新日期：2017-02-01 00:00:00
abstract：:Genomic sequencing reveals similar but limited numbers of protein-coding genes in different genomes, which begs the question of how organismal diversities are generated. Alternative pre-mRNA splicing, a widespread phenomenon in higher eukaryotic genomes, is thought to provide a mechanism to increase the complexity of ...
journal_title：Chromosoma
pub_type： 杂志文章,评审
doi：10.1007/s00412-013-0407-z
更新日期：2013-06-01 00:00:00
abstract：:Sister chromatids are physically connected by cohesin complexes. This sister chromatid cohesion is essential for the biorientation of chromosomes on the mitotic and meiotic spindle. In many species, cohesion between chromosome arms is partly dissolved in prophase of mitosis, whereas cohesion is protected at centromere...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/s00412-007-0139-z
更新日期：2008-04-01 00:00:00
abstract：:The distribution of quinacrine and protein sulphur has been compared with that of DNA in euchromatic and heterochromatic regions of mouse chromosomes stained with the fluorescent dye quinacrine, using X-ray microanalysis. Heterochromatin tends to bind relatively more quinacrine than euchromatin, and contains a greater...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00294059
更新日期：1985-01-01 00:00:00
abstract：:Saccharomyces cerevisiae cells containing null mutations in the SEP1 gene, which encodes the homologous pairing and strand exchange protein p175SEP1, enter pachytene with a delay. They arrest uniformly at this stage of meiotic prophase, probably revealing a checkpoint in the transition from pachytene to meiosis I. At ...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00352322
更新日期：1994-04-01 00:00:00
abstract：:The sex chromosomes in Sauropsida (reptiles and birds) have evolved independently many times. They show astonishing diversity in morphology ranging from cryptic to highly differentiated sex chromosomes with male (XX/XY) and female heterogamety (ZZ/ZW). Comparing such diverse sex chromosome systems thus provides unpara...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/s00412-015-0531-z
更新日期：2016-03-01 00:00:00
abstract：:We provide evidence that centromere-specific 155 bp DNA repeats terminate one pair of telomeres at the telocentric, left end of the short fourth chromosome in Chironomus pallidivittatus. Earlier evidence indicated that all other telomeres are terminated by 340 bp telomere-specific repeats. DNA that borders the 155 bp ...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/s00412-001-0176-y
更新日期：2002-02-01 00:00:00
abstract：:Formation of stable kinetochore-microtubule attachments is essential for accurate chromosome segregation in human cells and depends on the NDC80 complex. We recently showed that Chmp4c, an endosomal sorting complex required for transport protein involved in membrane remodelling, localises to prometaphase kinetochores ...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/s00412-018-0675-8
更新日期：2018-12-01 00:00:00
abstract：:The chironomid midge Pseudodiamesa branickii shows three polytene, banded elements in salivary gland and Malpighian tubule cells, none of which carry a nucleolar organizer. The present investigation revealed four chromosome pairs in mitotic metaphases, the nucleolus was localized in the tiny chromosome 4. Microphotome...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00292473
更新日期：1984-01-01 00:00:00
abstract：:Changes in the nuclear positioning of specific genes, depending on their expression status, have been observed in a large diversity of physiological processes. However, gene position is poorly documented for immune cells which have been subjected to activation following bacterial infection. Using a pig model, we focus...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/s00412-011-0328-7
更新日期：2011-10-01 00:00:00
abstract：:Macronuclear DNA was isolated from Paramecium primaurelia, stock 168. Although the macronucleus is polyploid to the extent of 840C, in other respect the DNA appears to be simply organized, having neither satellite sequences nor substantial amounts of intermediately repetitive sequence. The sequence complexity of macro...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00328389
更新日期：1980-01-01 00:00:00
abstract：:The influence of the sex-reversal factor (Sxr) on X and Y chromosome pairing was examined by comparing males with "novel" and "standard" Sxr genotypes. The novel Sxr males were exceptional in carrying Sxr on their X rather than their Y chromosome, or homozygously on both their X and Y chromosomes, or on a Y chromosome...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00292766
更新日期：1989-03-01 00:00:00
abstract：:The binding of methylene blue to DNA and chromatin treated in various ways was examined by equilibrium dialysis. The maximum r value (moles of bound dye/mole of nucleotide) was 1.0 for DNA, 0.6 for unfixed chromatin, and 0.83 for chromatin fixed in methanol-acetic acid. When fixed chromatin was treated with saline-cit...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00326323
更新日期：1975-08-11 00:00:00
abstract：:The haspins are divergent members of the eukaryotic protein kinase family that are conserved in many eukaryotic lineages including animals, fungi, and plants. Recently-solved crystal structures confirm that the kinase domain of human haspin has unusual structural features that stabilize a catalytically active conforma...
journal_title：Chromosoma
pub_type： 杂志文章,评审
doi：10.1007/s00412-009-0250-4
更新日期：2010-04-01 00:00:00
abstract：:The spindle checkpoint restrains anaphase onset and mitotic exit until all chromosomes are stably attached to the mitotic spindle via their kinetochores. The Tao1 protein kinase was recently reported as a novel spindle checkpoint component. When an siRNA was used to repress Tao1, the essential spindle checkpoint compo...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/s00412-010-0261-1
更新日期：2010-08-01 00:00:00
abstract：:In mammals, X-inactivation process is achieved by the cis-spreading of long noncoding Xist RNA over one of the female X chromosomes. The Xist binding accumulates histones H3 methylation and H4 hypoacetylation required for X inactivation that leads to proper dosage compensation of the X-linked genes. Co-transcription o...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/s00412-014-0495-4
更新日期：2015-06-01 00:00:00
abstract：:The W chromosome of the codling moth, Cydia pomonella, like that of most Lepidoptera species, is heterochromatic and forms a female-specific sex chromatin body in somatic cells. We collected chromatin samples by laser microdissection from euchromatin and W-chromatin bodies. DNA from the samples was amplified by degene...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/s00412-006-0086-0
更新日期：2007-04-01 00:00:00
abstract：:cDNA, copied from nuclear RNA isolated from heat shocked Drosophila hydei cells, has been cloned. From this collection of clones a clone, N09-15, with a 450 bp insert has been isolated that hybridizes in situ to the heat shock locus-2-48B of Drosophila hydei. The N09-15 sequence is present in two different genomic arr...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00294968
更新日期：1982-01-01 00:00:00
abstract：:The large-scale primary structure of the centromeric region of rice chromosome 5 was analyzed, the first example in a cereal species. The yeast artificial chromosome (YAC) and bacterial artificial chromosome (BAC) contigs aligned on the centromere of rice chromosome 5 (CEN5) covered a distance of more than 670 kb. Str...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/s004120100148
更新日期：2001-08-01 00:00:00
abstract：:The Balbiani rings in the salivary gland polytene chromosomes of Chironomus tentans include the most active structural genes in this organ. Two of them (BR1 and BR2) contain repetitive sequences and are transcribed into giant RNA molecules. On Southern blots of restriction digests, we have identified fragments of geno...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00327354
更新日期：1981-01-01 00:00:00
abstract：:Isolated nuclei of the unicellular alga Olisthodiscus luteus, the uninucleate dinoflagellate Crypthecodinium cohnii and the binucleate dinoflagellate Peridinium balticum were lysed and deposited on grids by the microcentrifugation technique. The ultrastructure of the released chromatin fibers was compared to that of m...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00292229
更新日期：1980-01-01 00:00:00
abstract：:We describe a novel rearranged human Y chromosome consisting of an inverted duplication of the long arm heterochromatin and a small amount of euchromatin: rea(Y)(qter-q11.2::q11.2-qter). The normal centromere has been deleted and a neocentromere containing CENP-A, -C, -E and Mad2 but not CENP-B has formed close to the...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/s004120000081
更新日期：2000-01-01 00:00:00
abstract：:Spermatogenesis in the F1 hybrid (2n=24=12 female + 12 male) between the closely related newt species T. cristatus carnifex and T. marmoratus was apparently normal up to pachytene. Many unpaired chromosomes were present at diplotene and a typical diakinesis was lacking. Primary spermatocytes at meta- and meta-anaphase...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00331572
更新日期：1979-08-10 00:00:00
abstract：:By using the optical properties of birefringence of DNA, the arrangement of these molecules has been studied in Dinoflagellate chromosomes and Dipteran polytene chromosomes. These latter are used, here, as a reference material. These observations have been made under a polarizing microscope on intact and stretched chr...
journal_title：Chromosoma
pub_type： 杂志文章
doi：10.1007/BF00330371
更新日期：1978-08-21 00:00:00
