Sequence of DNA replication in 277 R- and Q-bands of human chromosomes using a BrdU treatment.

abstract：:Spermatogenic cells of the mouse have been separated by gravity sedimentation using a modification of a previously published method. Details are given for the collection of purified samples of specific meiotic stages and for the collection of labelled cell fractions following injection of 3H-thymidine. Suppression of ...

journal_title：Chromosoma

authors： Chandley AC,Hotta Y,Stern H

更新日期：1977-07-08 00:00:00

abstract：:Sister chromatids are physically connected by cohesin complexes. This sister chromatid cohesion is essential for the biorientation of chromosomes on the mitotic and meiotic spindle. In many species, cohesion between chromosome arms is partly dissolved in prophase of mitosis, whereas cohesion is protected at centromere...

journal_title：Chromosoma

authors： Koch B,Kueng S,Ruckenbauer C,Wendt KS,Peters JM

更新日期：2008-04-01 00:00:00

abstract：:This paper describes the effects of 5-azacytidine on the condensation state of rye (Secale cereale L.) chromatin introduced into the wheat genome (Triticum aestivum L. cv. Beaver). The wheat cultivar Beaver carries a translocation between the short arm of rye chromosome 1R (1RS) and the long arm of wheat chromosome 1B...

journal_title：Chromosoma

authors： Glyn MC,Egertová M,Gazdova B,Kovarik A,Bezdek M,Leitch AR

更新日期：1997-12-01 00:00:00

abstract：:Nucleosome assembly involves deposition of a heterotetramer of histones H3/H4 onto DNA followed by two heterodimers of histones H2A/H2B. Cycles of nucleosome assembly and disassembly are essential to cellular events such as replication, transcription, and DNA repair. After synthesis in the cytoplasm, histones are shut...

journal_title：Chromosoma

authors： Mousson F,Ochsenbein F,Mann C

更新日期：2007-04-01 00:00:00

abstract：:The organization of interphase chromatin spans many topics, ranging in scale from the molecular level to the whole nucleus, and its study requires a concomitant range of experimental approaches. In this review, we examine these approaches, the results they have generated, and the interfaces between them. The greatest ...

journal_title：Chromosoma

authors： Horowitz-Scherer RA,Woodcock CL

更新日期：2006-02-01 00:00:00

abstract：:The large-scale primary structure of the centromeric region of rice chromosome 5 was analyzed, the first example in a cereal species. The yeast artificial chromosome (YAC) and bacterial artificial chromosome (BAC) contigs aligned on the centromere of rice chromosome 5 (CEN5) covered a distance of more than 670 kb. Str...

journal_title：Chromosoma

authors： Nonomura K,Kurata N

更新日期：2001-08-01 00:00:00

abstract：:Compelling evidence has been obtained in favour of the idea that the nuclear surface of higher plant cells is a microtubule-nucleating and/or organizing site (MTOC), in the absence of defined centrosomes. How these plant MTOC proteins are redistributed and function during the progression of the cell cycle remains enti...

journal_title：Chromosoma

authors： Schmit AC,Stoppin V,Chevrier V,Job D,Lambert AM

更新日期：1994-09-01 00:00:00

abstract：:Short DNA regions, known to contain replication origins, were isolated from 2 M NaCl resistant nuclear structures of Physarum polycephalum after predigestion with DNase. Regions of 100 bp average length were cloned and sequenced. About 25% of the clones contained direct repeats of 12 to 16 bp and variable base sequenc...

journal_title：Chromosoma

authors： Opstelten RJ,Clement JM,Wanka F

更新日期：1989-12-01 00:00:00

abstract：:In wild-type Drosophila hydei (genotype X/Y) four different primary spermatocyte nuclear glycoproteins, classified as non-Y encoded because of their occurrence in X/O genotypes, were demonstrated to possess a few epitopes that depended on formation of the Y chromosomal giant lampbrush loops threads (th; Mr 55,000 prot...

journal_title：Chromosoma

authors： Tischendorf G,Liebrich W,Trapitz P,Wood G,Schwochau M

更新日期：1989-08-01 00:00:00

abstract：:cDNA, copied from nuclear RNA isolated from heat shocked Drosophila hydei cells, has been cloned. From this collection of clones a clone, N09-15, with a 450 bp insert has been isolated that hybridizes in situ to the heat shock locus-2-48B of Drosophila hydei. The N09-15 sequence is present in two different genomic arr...

journal_title：Chromosoma

authors： Peters FP,Grond CJ,Sondermeijer PJ,Lubsen NH

更新日期：1982-01-01 00:00:00

abstract：:Simple repeated GATA and GACA sequences which were originally isolated from sex-specific snake satellite DNA have been found subsequently in all eukaryotes studied. The organization of these sequences within the mouse genome was investigated here by using synthetic oligonucleotide probes as a novel tool in comparison ...

journal_title：Chromosoma

authors： Schäfer R,Ali S,Epplen JT

更新日期：1986-01-01 00:00:00

abstract：:Two families of tandemly repeated satellite DNAs were isolated from the neotropical primate Callimico goeldii (Goeldi's marmoset). One satellite, CgoA, is over 70% A + T and has a monomer length of 338 bp. The other satellite, CgoB, is 50% A + T and has a monomer length of 916 bp. Both CgoA and CgoB hybridize strongly...

journal_title：Chromosoma

authors： Fanning TG,Seuánez HN,Forman L

更新日期：1989-12-01 00:00:00

abstract：:Allopolyploidy and homoeologous recombination are two important processes in reshaping genomes and generating evolutionary novelties. Newly formed allopolyploids usually display chromosomal perturbations as a result of pairing errors at meiosis. To understand mechanisms of stabilization of allopolyploid species derive...

journal_title：Chromosoma

authors： Wang Y,Zhao Q,Qin X,Yang S,Li Z,Li J,Lou Q,Chen J

更新日期：2017-12-01 00:00:00

abstract：:Macrosatellites are large polymorphic tandem arrays. The human subtelomeric macrosatellite D4Z4 has 11-150 repeats, each containing a copy of the intronless DUX4 gene. DUX4 is linked to facioscapulohumeral muscular dystrophy, but its normal function is unknown. The DUX gene family includes DUX4, the intronless Dux mac...

journal_title：Chromosoma

authors： Leidenroth A,Clapp J,Mitchell LM,Coneyworth D,Dearden FL,Iannuzzi L,Hewitt JE

更新日期：2012-10-01 00:00:00

abstract：:Human satellite DNAs I, II and IV were transcribed to yield radioactive complementary RNAs (cRNAs). These cRNAs were hybridised to metaphase chromosomes of man, chimpanzee (Pan troglodytes), gorilla (Gorilla gorilla) and orang utan (Pongo pygmaeus). The results of this in situ hybridisation were analysed quantitativel...

journal_title：Chromosoma

authors： Gosden JR,Mitchell AR,Seuanez HN,Gosden CM

更新日期：1977-09-27 00:00:00

abstract：:Lack of crossing-over in meiosis can trigger an apoptotic response at metaphase I by the spindle assembly checkpoint (SAC). In contrast to females, segregation of sex chromosomes in males poses a particular challenge as recombination and chiasma formation is restricted to the pseudoautosomal region, the small region o...

journal_title：Chromosoma

authors： Faisal I,Kauppi L

更新日期：2016-06-01 00:00:00

abstract：:The interaction of Hoechst 33258 with DNA has been examined to help clarify the mechanisms of banding. 1. In agreement with previous studies Hoechst fluorescence is enhanced to a greater degree in AT-rich compared to GC-rich DNA. 2. Hoechst causes an increase in the DNA Tm which is greater at the higher AT content of ...

journal_title：Chromosoma

authors： Comings DE

更新日期：1975-10-14 00:00:00

abstract：:With an improved N-banding technique, the location of nucleolar organizing region was determined in 27 kinds of material including mammals, a marsupial, birds, amphibians, fishes, an insect and plants. In most cases the N-bands were clearly located on certain specific regions of chromosomes, such as the secondary cons...

journal_title：Chromosoma

authors： Funaki K,Matsui S,Sasaki M

更新日期：1975-01-01 00:00:00

abstract：:Macronuclear DNA was isolated from Paramecium primaurelia, stock 168. Although the macronucleus is polyploid to the extent of 840C, in other respect the DNA appears to be simply organized, having neither satellite sequences nor substantial amounts of intermediately repetitive sequence. The sequence complexity of macro...

journal_title：Chromosoma

authors： McTavish C,Sommerville J

更新日期：1980-01-01 00:00:00

abstract：:Homologous chromosomes initially undergo weak alignments that bring homologous sequences into register during meiosis. These alignments can be facilitated by two types of mechanisms: interstitial homology searches and telomere-telomere alignments. As prophase (and chromatin compaction) proceeds, these initial pairings...

journal_title：Chromosoma

authors： Walker MY,Hawley RS

更新日期：2000-01-01 00:00:00

abstract：:Polo-like kinase 1 (Plk1) is an essential mitotic regulator and undergoes periodic phosphorylation on threonine 210, a conserved residue in the kinase's activation loop. While phosphate-mimicking alterations of T210 stimulate Plk1's kinase activity in vitro, their effects on cell cycle regulation in vivo remain contro...

journal_title：Chromosoma

authors： Paschal CR,Maciejowski J,Jallepalli PV

更新日期：2012-12-01 00:00:00

abstract：:A system has been developed using a microscope photometer to measure the DNA content of individual chromosomes. After correcting for the systematic and statistical errors the measured values are highly reproducible. Data are presented of measurements of human Y chromosomes from a cytogenetically normal population. Whe...

journal_title：Chromosoma

authors： Wall WJ,Butler LJ

更新日期：1989-01-01 00:00:00

abstract：:BrdU-33258 Hoechst techniques have been used to characterize DNA replication patterns in lymphocytes from hunam females with supernumerary or structurally abnormal X chromosomes. Fluorescence analysis permits identification of late replicating X chromosomes in a very high proportion of cells and affords a high resolut...

journal_title：Chromosoma

authors： Latt SA,Willard HF,Gerald PS

更新日期：1976-08-17 00:00:00

abstract：:The mean DNA content of G2 macronuclei varies during the life cycle of the ciliate Tetrahymena thermophila. Early in the life cycle the mean is about 130 C; later it is about 94 C. In hybrids between strains A and B the decrease from 130 C to 94 C usually began after 60 fissions after conjugation. In B X B clones the ...

journal_title：Chromosoma

authors： Doerder FP,DeBault LE

更新日期：1978-10-20 00:00:00

abstract：:Eukaryotic genomes are organized in large chromatin domains that maintain proper gene activity in the cell. These domains may be permissive or repressive to the transcription of underlying genes. Based on its protein makeup, chromatin in Drosophila cell culture has been recently categorized into five color-coded state...

journal_title：Chromosoma

authors： Maksimov DA,Koryakov DE,Belyakin SN

更新日期：2014-06-01 00:00:00

abstract：:We have used a new approach involving in situ hybridisation and electron microscopy to establish ultrastructural homologies between polytene chromosome regions of Drosophila melanogaster and Drosophila subobscura. Twelve probes were chosen to cover all the chromosomal elements: the myospheroid gene, the collagen type ...

journal_title：Chromosoma

authors： Cuenca JB,Galindo MI,Saura AO,Sorsa V,de Frutos R

更新日期：1998-05-01 00:00:00

abstract：:In Drosophila, the Polycomb group and trithorax group proteins play a critical role in controlling the expression states of homeotic gene complexes during development. The common view is that these two classes of proteins bind to the homeotic complexes and regulate transcription at the level of chromatin. In the prese...

journal_title：Chromosoma

authors： Fanti L,Perrini B,Piacentini L,Berloco M,Marchetti E,Palumbo G,Pimpinelli S

更新日期：2008-02-01 00:00:00

abstract：:In mammals, paternal and maternal pronuclei undergo profound chromatin reorganisation upon fertilisation. How these events are orchestrated within centromeric regions to ensure proper chromosome segregation in the following cellular divisions is unknown. In this study, we followed the dynamic unfolding of the centrome...

journal_title：Chromosoma

authors： Probst AV,Santos F,Reik W,Almouzni G,Dean W

更新日期：2007-08-01 00:00:00

abstract：:X chromosome inactivation (XCI) is an essential mechanism to compensate gene dosage in mammals. Here, we show that XCI has evolved differently in two species of the genus Tokudaia. The Amami spiny rat, Tokudaia osimensis, has a single X chromosome in males and females (XO/XO). By contrast, the Okinawa spiny rat, Tokud...

journal_title：Chromosoma

authors： Zushi H,Murata C,Mizushima S,Nishida C,Kuroiwa A

更新日期：2017-12-01 00:00:00

abstract：:Genomic sequencing reveals similar but limited numbers of protein-coding genes in different genomes, which begs the question of how organismal diversities are generated. Alternative pre-mRNA splicing, a widespread phenomenon in higher eukaryotic genomes, is thought to provide a mechanism to increase the complexity of ...

journal_title：Chromosoma

authors： Zhou Z,Fu XD

更新日期：2013-06-01 00:00:00