A group of non-Y encoded Drosophila hydei primary spermatocyte nuclear glycoproteins exhibits epitopes depending on formation of Y chromosomal giant lampbrush loops.

abstract：:Lack of crossing-over in meiosis can trigger an apoptotic response at metaphase I by the spindle assembly checkpoint (SAC). In contrast to females, segregation of sex chromosomes in males poses a particular challenge as recombination and chiasma formation is restricted to the pseudoautosomal region, the small region o...

journal_title：Chromosoma

authors： Faisal I,Kauppi L

更新日期：2016-06-01 00:00:00

abstract：:Recent data suggest that insulators organize chromatin architecture in the nucleus. The best characterized Drosophila insulator, found in the gypsy retrotransposon, contains 12 binding sites for the Su(Hw) protein. Enhancer blocking, along with Su(Hw), requires BTB/POZ domain proteins, Mod(mdg4)-67.2 and CP190. Inacti...

journal_title：Chromosoma

authors： Melnikova L,Shapovalov I,Kostyuchenko M,Georgiev P,Golovnin A

更新日期：2017-03-01 00:00:00

abstract：:To structurally dissect mitotic chromosomes, we aim to position along the folded chromatin fiber proteins involved in long-range order, such as topoisomerase IIalpha (topoIIalpha) and condensin. Immuno-electron microscopy (EM) of thin-sectioned chromosomes is the method of choice toward this goal. A much-improved immu...

journal_title：Chromosoma

authors： Maeshima K,Eltsov M,Laemmli UK

更新日期：2005-11-01 00:00:00

abstract：:The study of RNA pol II-mediated transcription regulation has been dominated by molecular biological approaches. Although these methods continue to provide important insights, other approaches are required to insure against an oversimplified view of gene expression. Improvements in EM methods and the development of th...

journal_title：Chromosoma

authors： Hendzel MJ,Bazett-Jones DP

更新日期：1995-02-01 00:00:00

abstract：:Homologous chromosomes initially undergo weak alignments that bring homologous sequences into register during meiosis. These alignments can be facilitated by two types of mechanisms: interstitial homology searches and telomere-telomere alignments. As prophase (and chromatin compaction) proceeds, these initial pairings...

journal_title：Chromosoma

authors： Walker MY,Hawley RS

更新日期：2000-01-01 00:00:00

abstract：:Heterochromatin in eukaryotic interphase cells frequently localizes to the nucleolar periphery (nucleolus-associated domains (NADs)) and the nuclear lamina (lamina-associated domains (LADs)). Gene expression in somatic cell NADs is generally low, but NADs have not been characterized in mammalian stem cells. Here, we g...

journal_title：Chromosoma

authors： Bizhanova A,Yan A,Yu J,Zhu LJ,Kaufman PD

更新日期：2020-06-01 00:00:00

abstract：:We determined the structure of the Schizosaccharomyces pombe centromere cen3 using direct genomic mapping and cosmid walking. The repetitive region of cen3 is approximately 110 kb, much longer than that of the previously determined cen1 and cen2 regions. The approximately 30 kb long left and approximately 60 kb right ...

journal_title：Chromosoma

authors： Murakami S,Matsumoto T,Niwa O,Yanagida M

更新日期：1991-12-01 00:00:00

abstract：:Targeted insertion of large pieces of DNA is an important goal of genetic engineering. However, this goal has been elusive since classical methods for homology-directed repair are inefficient and often not feasible in many systems. Recent advances are described here that enable site-specific genomic insertion of relat...

journal_title：Chromosoma

authors： Yamamoto Y,Gerbi SA

更新日期：2018-12-01 00:00:00

abstract：:Three new 18S.26S rRNA gene loci were identified in common wheat by sequential N-banding and in situ hybridization (ISH) analysis. Locus Nor-A7 is located at the terminal area of the long arm of 5A in both diploid and polyploid wheats. Locus Nor-B6 is located in N-band 1BL2.5 of the long arm of chromosome 1B in Tritic...

journal_title：Chromosoma

authors： Jiang J,Gill BS

更新日期：1994-06-01 00:00:00

abstract：:The cotyledon parenchyma cells of common bean (Phaseolus vulgaris L.) produce large quantities of storage proteins during embryo maturation; throughout this period, these cells also accumulate nuclear DNA (nDNA). To investigate the basis of this nDNA accumulation, we have measured storage protein mRNA pools, nDNA mass...

journal_title：Chromosoma

authors： Johnson KA,Sussex IM

更新日期：1990-07-01 00:00:00

abstract：:Sugar beet (Beta vulgaris) chromosomes consist of large heterochromatic blocks in pericentromeric, centromeric, and intercalary regions comprised of two different highly abundant DNA satellite families. To investigate DNA methylation at single base resolution at heterochromatic regions, we applied a method for strand-...

journal_title：Chromosoma

authors： Zakrzewski F,Weisshaar B,Fuchs J,Bannack E,Minoche AE,Dohm JC,Himmelbauer H,Schmidt T

更新日期：2011-08-01 00:00:00

abstract：:After selective enrichment and differential hybridisation of Cot-1 DNA fractions of plants with and without polymorphic heterochromatic segments, a repetitive sequence (called Bds1) specific to the polymorphic chromosome segments of Brachycome dichromosomatica (Brachyscome dichromosomatica) was isolated. A single repe...

journal_title：Chromosoma

authors： Houben A,Wanner G,Hanson L,Verlin D,Leach CR,Timmis JN

更新日期：2000-06-01 00:00:00

abstract：:Nuclear and cytoplasmic RNA of Stylonychia mytilus were analyzed on denaturing polyacrylamide gels. The molecular weight of rRNA precursor molecules is within a range of 2.1 x 10(6) daltons. A comparison between the electrophoretic pattern of nuclear non-ribosomal RNA and cytoplasmic mRNA indicates that a considerable...

journal_title：Chromosoma

authors： Nock A

更新日期：1981-01-01 00:00:00

abstract：:The divergence of lineages leading to extant squamate reptiles (lizards, snakes, and amphisbaenians) and birds occurred about 275 million years ago. Birds, unlike squamates, have karyotypes that are typified by the presence of a number of very small chromosomes. Hence, a number of chromosome rearrangements might be ex...

journal_title：Chromosoma

authors： Pokorná M,Giovannotti M,Kratochvíl L,Kasai F,Trifonov VA,O'Brien PC,Caputo V,Olmo E,Ferguson-Smith MA,Rens W

更新日期：2011-10-01 00:00:00

abstract：:The appearance of RNP droplets associated with specific regions of polytene chromosomes from Chironomus larvae has been studied, systematically by light microscopy using glutarladehyde fixation and lactic acid squashing of the salivary gland. During the fourth larval instar the highest frequency of nuclei which contai...

journal_title：Chromosoma

authors： Stockert JC,Díez JL

更新日期：1979-09-01 00:00:00

abstract：:Polo-like kinase 1 (Plk1) is an essential mitotic regulator and undergoes periodic phosphorylation on threonine 210, a conserved residue in the kinase's activation loop. While phosphate-mimicking alterations of T210 stimulate Plk1's kinase activity in vitro, their effects on cell cycle regulation in vivo remain contro...

journal_title：Chromosoma

authors： Paschal CR,Maciejowski J,Jallepalli PV

更新日期：2012-12-01 00:00:00

abstract：:Using indirect immunofluorescence visualization techniques we investigated the distribution of RNA polymerase B (or II) and histone H1 at heat shock puff loci in Drosophila melanogaster polytene chromosomes at different times during and after heat shock. After heat treatments of from 5 to 45 min, the heat shock puff d...

journal_title：Chromosoma

authors： Greenleaf AL,Plagens U,Jamrich M,Bautz EK

更新日期：1978-01-16 00:00:00

abstract：:The hypothesis that the genome is composed of a patchwork of structural and functional domains (units) that may be either active or repressed was proposed almost 30 years ago. Here, we examine the evolution of the domain model of eukaryotic genome organization in view of the expansion of genome-scale techniques in the...

journal_title：Chromosoma

authors： Razin SV,Vassetzky YS

更新日期：2017-02-01 00:00:00

abstract：:The ability to reproduce is a major trait of living organisms. This ability is carried out by specialized reproductive cells-gametes. In mammals, gametes develop through a unique developmental pathway. Extensive changes in the epigenome of gametes occur during embryonic development. With birth, gametes continue to mat...

journal_title：Chromosoma

authors： Wasserzug-Pash P,Klutstein M

更新日期：2019-09-01 00:00:00

abstract：:In Xenopus laevis eight tRNA genes are located in a 3.18 kb tandemly repeated unit. There are 150 copies of the unit at a single locus near the long arm telomere of one of the acrocentric chromosomes in the 14-17 group. Two additional classes of tRNA gene-containing repeats have been isolated (defined by clones p3.1 a...

journal_title：Chromosoma

authors： Narayanswami S,Doering JL,Fokta FJ,Rosenthal DS,Nguyen TN,Hamkalo BA

更新日期：1995-10-01 00:00:00

abstract：:Ribosomal DNA nontranscribed spacers in Drosophila virilis DNA have been examined in some detail by restriction site analysis of cloned segments of rDNA, nucleic acid hybridizations involving unfractionated rDNA, and base composition estimates. The overall G + C content of the spacer is 27--28%; this compares with 39%...

journal_title：Chromosoma

authors： Rae PM,Barnett T,Murtif VL

更新日期：1981-01-01 00:00:00

abstract：:Macrosatellites are large polymorphic tandem arrays. The human subtelomeric macrosatellite D4Z4 has 11-150 repeats, each containing a copy of the intronless DUX4 gene. DUX4 is linked to facioscapulohumeral muscular dystrophy, but its normal function is unknown. The DUX gene family includes DUX4, the intronless Dux mac...

journal_title：Chromosoma

authors： Leidenroth A,Clapp J,Mitchell LM,Coneyworth D,Dearden FL,Iannuzzi L,Hewitt JE

更新日期：2012-10-01 00:00:00

abstract：:The distinctive behaviors of chromosomes in mitosis and meiosis depend upon differences in kinetochore position. Kinetochore position is well established except for a critical transition between meiosis I and meiosis II. We examined kinetochore position during the transition and compared it with the position of kineto...

journal_title：Chromosoma

authors： Paliulis LV,Nicklas RB

更新日期：2005-03-01 00:00:00

abstract：:Ring chromosomes are of basic interest to the geneticist and cell biologist who study their behavior in meiotic and mitotic divisions. In addition, the mitotic instability associated with some ring-X chromosomes has proven useful in Drosophila as a means to produce gynandromorphs for developmental studies. We describe...

journal_title：Chromosoma

authors： Golic MM,Golic KG

更新日期：2011-04-01 00:00:00

abstract：:The chironomid midge Pseudodiamesa branickii shows three polytene, banded elements in salivary gland and Malpighian tubule cells, none of which carry a nucleolar organizer. The present investigation revealed four chromosome pairs in mitotic metaphases, the nucleolus was localized in the tiny chromosome 4. Microphotome...

journal_title：Chromosoma

authors： Zacharias H

更新日期：1984-01-01 00:00:00

abstract：:The method of ultrathin sections of unsquashed salivary gland polytene chromosomes of Ch. thummi was applied to their ultrastrual mapping. There was a good agreement between electron micrographs and Hägele's light microscopic map (1970) with respect to the pattern and number of bands. 94% of bands were identified in l...

journal_title：Chromosoma

authors： Kiknadze II,Perov NA,Chentsov YS

更新日期：1976-03-31 00:00:00

abstract：:Membrane-free folded chromosomes were purified from log-phase cultures of Escherichia coli and prepared for electron microscopy by aqueous (Kleinschmidt and Zahn) spreading. The appearance of the chromosomes depended on the salt concentrations in spreading. At certain salt concentration, the chromosomes resembled rose...

journal_title：Chromosoma

authors： Kavenoff R,Bowen BC

更新日期：1976-12-16 00:00:00

abstract：:Chromosomes were subjected to either prolonged hypotonic solution pretreatment or aging. Both conditions greatly loosened and dispersed the overlying epichromatin from the central chromosome core structure. This was followed by silver staining and examination with bright-field microscopy. The chromosome core selective...

journal_title：Chromosoma

authors： Howell WM,Hsu TC

更新日期：1979-06-21 00:00:00

abstract：:One of the prominent cell cycle-related modifications of histone proteins whose function remains unresolved is the phosphorylation of linker histone H1. In this work we have used indirect immunofluorescence on human cells with antibodies that are specific for phosphorylated histone H1 to examine the cellular distribut...

journal_title：Chromosoma

authors： Boggs BA,Allis CD,Chinault AC

更新日期：2000-03-01 00:00:00

abstract：:We describe a novel rearranged human Y chromosome consisting of an inverted duplication of the long arm heterochromatin and a small amount of euchromatin: rea(Y)(qter-q11.2::q11.2-qter). The normal centromere has been deleted and a neocentromere containing CENP-A, -C, -E and Mad2 but not CENP-B has formed close to the...

journal_title：Chromosoma

authors： Floridia G,Gimelli G,Zuffardi O,Earnshaw WC,Warburton PE,Tyler-Smith C

更新日期：2000-01-01 00:00:00