Abstract:
:Using indirect immunofluorescence visualization techniques we investigated the distribution of RNA polymerase B (or II) and histone H1 at heat shock puff loci in Drosophila melanogaster polytene chromosomes at different times during and after heat shock. After heat treatments of from 5 to 45 min, the heat shock puff displayed intense fluorescence when stained for RNA polymerase B, but relatively little fluorescence when stained for histone H1. Returning heat shocked larvae to room temperature resulted in the appearance of a distinctive pattern of RNA polymerase-associated fluorescence in the heat shock puff at 87C, presumably reflecting events associated with the inactivation and regression of this puff. Large differences observed in the apparent RNA polymerase B content of puffs of similar size suggest that the interaction of RNA polymerase B with chromosomal loci does not depend on simply the state of condensation or decondensation of the chromatin.
journal_name
Chromosomajournal_title
Chromosomaauthors
Greenleaf AL,Plagens U,Jamrich M,Bautz EKdoi
10.1007/BF00329465subject
Has Abstractpub_date
1978-01-16 00:00:00pages
127-36issue
2eissn
0009-5915issn
1432-0886journal_volume
65pub_type
杂志文章相关文献
CHROMOSOMA文献大全abstract::We describe a novel rearranged human Y chromosome consisting of an inverted duplication of the long arm heterochromatin and a small amount of euchromatin: rea(Y)(qter-q11.2::q11.2-qter). The normal centromere has been deleted and a neocentromere containing CENP-A, -C, -E and Mad2 but not CENP-B has formed close to the...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s004120000081
更新日期:2000-01-01 00:00:00
abstract::We have studied the diameter of chromatin fibres embedded in epoxy resins for three different materials: mouse thymus, chicken erythrocytes and sea cucumber spermatozoa. We confirm that the diameter of chromatin fibres increases with linker length, both values being influenced by the protein composition of chromatin. ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00293338
更新日期:1989-06-01 00:00:00
abstract::The study of RNA pol II-mediated transcription regulation has been dominated by molecular biological approaches. Although these methods continue to provide important insights, other approaches are required to insure against an oversimplified view of gene expression. Improvements in EM methods and the development of th...
journal_title:Chromosoma
pub_type: 杂志文章,评审
doi:10.1007/BF00355315
更新日期:1995-02-01 00:00:00
abstract::Position-effect variegation (PEV) is the mosaic expression of a euchromatic gene brought into juxtaposition with heterochromatin. Fourteen different transformed Drosophila melanogaster lines with variegating P-element inserts were used to examine the DNA levels of these transgenes. Insert sites include pericentric, te...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00352116
更新日期:1996-04-01 00:00:00
abstract::Chromosomal replication was studied by means of the BrdU-Höchst-Giemsa-technique in three minute T-cell leukemias with special regard to chromosome 15. In all the three leukemic cell lines the normally early replicating band 15 E was undetectable whereas late replication in this region is completely unaltered. Further...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00288676
更新日期:1982-01-01 00:00:00
abstract::A complete human metaphase chromosome has been reconstructed from a series of electron microscopical projections obtained by tilting the specimen stage at 3 degree intervals from -60 to +60 degrees. The reconstructed structure is about 3.0 microns long, 1.6 micron wide, and 0.8 micron thick. The mass distribution was ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00293184
更新日期:1987-01-01 00:00:00
abstract::Microspore culture stimulates immature pollen grains to develop into plants via tissue culture and is used routinely in many crop species to produce "doubled haploids": homozygous, true-breeding lines. However, microspore culture is also often used on material that does not have stable meiosis, such as interspecific h...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-019-00720-w
更新日期:2019-12-01 00:00:00
abstract::With simultaneous immunofluorescence and fluorescent in situ hybridization, we have determined the organization of native and heterologous DNA sequences relative to the cores of meiotic prophase chromosomes. The normal chromatin organization is demonstrated with probes of mouse sequences: a cosmid probe that identifie...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00362284
更新日期:1994-10-01 00:00:00
abstract::PICH (Plk1-interacting checkpoint helicase) was recently identified as an essential component of the spindle assembly checkpoint and shown to localize to kinetochores, inner centromeres, and thin threads connecting separating chromosomes even during anaphase. In this paper, we have used immuno-fiber fluorescence in si...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-007-0131-7
更新日期:2008-04-01 00:00:00
abstract::DNA reassociation kinetics were studied, by means of the hydroxyapatite chromatography method, for four species of Amphibians with different nuclear DNA content: Xenopus laevis (3 pg DNA per haploid genome) and Bufo bufo (7 pg) of the Anura subclass and Triturus cristatus (23 pg) and Necturus maculosus (52 pg) of the ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00327706
更新日期:1976-12-06 00:00:00
abstract::Fluorescence in situ hybridization (FISH), especially chromosome painting, has been extensively exploited in the phylogenetic reconstruction of primate evolution. Although chromosome painting is a key method to map translocations, it is not effective in detecting chromosome inversions, which may be up to four times mo...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-015-0565-2
更新日期:2016-09-01 00:00:00
abstract::The overall nuclease sensitivity and methylation of active and inactive X chromosomes of kangaroos were examined by in situ nick translation. Cultured fibroblasts of subspecies wallaroo-euro (Macropus robustus robustus; Macropus robustus erubescens) hybrids were used, enabling the paternally and maternally derived X c...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00356024
更新日期:1993-01-01 00:00:00
abstract::The W chromosome of the codling moth, Cydia pomonella, like that of most Lepidoptera species, is heterochromatic and forms a female-specific sex chromatin body in somatic cells. We collected chromatin samples by laser microdissection from euchromatin and W-chromatin bodies. DNA from the samples was amplified by degene...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-006-0086-0
更新日期:2007-04-01 00:00:00
abstract::Minute elements detected in Megaselia scalaris (Phoridae, Diptera) lack chromosome arms but carry centromeres and possess kinetochore microtubules in mitosis as well as in meiosis. These centromere-like elements (CLEs) were present in two geographically independent strains of the fly. This indicates that their origin ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00357059
更新日期:1991-11-01 00:00:00
abstract::BrdU-substituted Chinese hamster chromosomes were treated with a hog Na2HPO4 solution and stained with Giemsa to produce sister chromatid differential staining (SCD). The process of SCD was examined with the Nomarski differential interference microscope and the scanning electron microscope. After the Na2HPO4 treatment...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00285754
更新日期:1981-01-01 00:00:00
abstract::The frequency and distribution of chiasmata and the nature of terminal "associations" was re-examined in Syrian hamster spermatocytes using the 5-bromo-2'-deoxyuridine (BrdU) antibody technique (BAT) for differential chromatid labelling. Differential chromatid substitution was achieved by BrdU incorporation at the pen...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF01731702
更新日期:1990-08-01 00:00:00
abstract::The polypurine/polypyrimidine (PuPy) tracts present in the human genome are known to be scattered among and within chromosomes. In PuPy tract sequences, triplex formation occurs readily under physiological conditions, leaving single-stranded DNAs capable of hybridization with complementary single-stranded DNAs and RNA...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-002-0198-0
更新日期:2002-09-01 00:00:00
abstract::The distribution of intermicrotubular bridges in spindles of tipulid spermatocytes (Pales ferruginea, first meiotic division) was analyzed using serial sections of pre-selected cells. Bridges were found in all spindle regions, including kinetochore microtubules and free microtubules in the chromosome fiber. The dimens...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00328643
更新日期:1986-01-01 00:00:00
abstract::Micronuclei are extra-nuclear bodies mainly derived from ana-telophase lagging chromosomes/chromatins (LCs) that are not incorporated into primary nuclei at mitotic exit. Unlike primary nuclei, most micronuclei are enclosed by nuclear envelope (NE) that is highly susceptible to spontaneous and irreparable rupture. Rup...
journal_title:Chromosoma
pub_type: 杂志文章,评审
doi:10.1007/s00412-020-00741-w
更新日期:2020-12-01 00:00:00
abstract::One of the prominent cell cycle-related modifications of histone proteins whose function remains unresolved is the phosphorylation of linker histone H1. In this work we have used indirect immunofluorescence on human cells with antibodies that are specific for phosphorylated histone H1 to examine the cellular distribut...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s004120050400
更新日期:2000-03-01 00:00:00
abstract::The nuclear topography of pericentromeric DNA of chromosome 11 was analyzed in G0 (nonstimulated) and G1 [phytohemagglutinin (PHA) stimulated] human lymphocytes by confocal microscopy. In addition to the nuclear center, the centrosome was used as a second point of reference in the three-dimensional (3D) analysis. Peri...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00352253
更新日期:1994-07-01 00:00:00
abstract::The salivary chromosomes of the l(3)tl mutant of D. melanogaster are considerably thicker and shorter than those of normal larvae. In most nuclei, chromosome shortening is associated with morphological changes of two types. a) The bands lose their distinctive pattern and become loose. The chromosome appears as a granu...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF01798344
更新日期:1976-04-21 00:00:00
abstract::The ribosome is responsible for the final step of decoding genetic information into proteins. Therefore, correct assembly of ribosomes is a fundamental task for all living cells. In eukaryotes, the construction of the ribosome which begins in the nucleolus requires coordinated efforts of >350 specialized factors that ...
journal_title:Chromosoma
pub_type: 杂志文章,评审
doi:10.1007/s00412-014-0463-z
更新日期:2014-08-01 00:00:00
abstract::In Drosophila, the Polycomb group and trithorax group proteins play a critical role in controlling the expression states of homeotic gene complexes during development. The common view is that these two classes of proteins bind to the homeotic complexes and regulate transcription at the level of chromatin. In the prese...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-007-0123-7
更新日期:2008-02-01 00:00:00
abstract::Saccharomyces cerevisiae cells containing null mutations in the SEP1 gene, which encodes the homologous pairing and strand exchange protein p175SEP1, enter pachytene with a delay. They arrest uniformly at this stage of meiotic prophase, probably revealing a checkpoint in the transition from pachytene to meiosis I. At ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00352322
更新日期:1994-04-01 00:00:00
abstract::We have used a new approach involving in situ hybridisation and electron microscopy to establish ultrastructural homologies between polytene chromosome regions of Drosophila melanogaster and Drosophila subobscura. Twelve probes were chosen to cover all the chromosomal elements: the myospheroid gene, the collagen type ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s004120050287
更新日期:1998-05-01 00:00:00
abstract::Very few of the middle repetitive DNA sequences found in Drosophila melanogaster are present in all of the species of the D. melanogaster subgroup. One member of the subgroup, D. erecta, lacks most of the families of repetitive elements from D. melanogaster (including copia and 412) but possesses many other families n...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00327329
更新日期:1983-01-01 00:00:00
abstract::Meiotic pairing and segregation were studied in three different heterozygous reciprocal translocation strains of the baker's yeast, Saccharomyces cerevisiae. Pachytene translocation quadrivalents were identified by a combination of immunofluorescence and fluorescence in situ hybridization and the karyotypes of meiotic...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s004120050304
更新日期:1998-09-01 00:00:00
abstract::Coilin, a molecular marker for Cajal bodies (CBs), is a phosphoprotein that contains a cryptic nucleolar localization signal and multiple interacting domains, such as the RG-box. Post-translational symmetrical dimethylation of arginines on the coilin RG-box is required for the recruitment of the survival motor neuron ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-010-0276-7
更新日期:2010-10-01 00:00:00
abstract::A karyotype based on banding pattern and chromosome length is presented for the white-handed gibbon, Hylobates lar. Little homology with the banding patterns of the chromosomes of the other Hominoidea can be seen, confirming the early evolutionary separation of Hylobatidae and the other apes. Hybridization in situ wit...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00285805
更新日期:1975-01-01 00:00:00