Abstract:
:A complete human metaphase chromosome has been reconstructed from a series of electron microscopical projections obtained by tilting the specimen stage at 3 degree intervals from -60 to +60 degrees. The reconstructed structure is about 3.0 microns long, 1.6 micron wide, and 0.8 micron thick. The mass distribution was fairly homogeneous within the chromatids and neither a hollow nor a dense core was observed. The distribution and course of fibers observed are most consistent with a looping model of chromosome structure.
journal_name
Chromosomajournal_title
Chromosomaauthors
Harauz G,Borland L,Bahr GF,Zeitler E,van Heel Mdoi
10.1007/BF00293184subject
Has Abstractpub_date
1987-01-01 00:00:00pages
366-74issue
5eissn
0009-5915issn
1432-0886journal_volume
95pub_type
杂志文章相关文献
CHROMOSOMA文献大全abstract::Rates of movement of univalents at prometaphase and of half-bivalents at anaphase in living cricket and grasshopper spermatocytes were determined as a function of the distance from the pole toward which the movement was directed. In the artificially produced univalents of cricket cells, correlation coefficients for ra...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00329921
更新日期:1978-11-22 00:00:00
abstract::BrdU-33258 Hoechst techniques have been used to characterize DNA replication patterns in lymphocytes from hunam females with supernumerary or structurally abnormal X chromosomes. Fluorescence analysis permits identification of late replicating X chromosomes in a very high proportion of cells and affords a high resolut...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00292912
更新日期:1976-08-17 00:00:00
abstract::Nuclear and cytoplasmic RNA of Stylonychia mytilus were analyzed on denaturing polyacrylamide gels. The molecular weight of rRNA precursor molecules is within a range of 2.1 x 10(6) daltons. A comparison between the electrophoretic pattern of nuclear non-ribosomal RNA and cytoplasmic mRNA indicates that a considerable...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00286790
更新日期:1981-01-01 00:00:00
abstract::From a "shotgun" collection of DNA fragments, isolated from Drosophila melanogaster, we selected sequences that function as autonomously replicating sequences (ARS) in the yeast Saccharomyces cerevisiae. To investigate the replicative potential of such sequences in Drosophila, five of these ARS elements and also the A...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00344161
更新日期:1991-05-01 00:00:00
abstract::The W chromosome of the codling moth, Cydia pomonella, like that of most Lepidoptera species, is heterochromatic and forms a female-specific sex chromatin body in somatic cells. We collected chromatin samples by laser microdissection from euchromatin and W-chromatin bodies. DNA from the samples was amplified by degene...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-006-0086-0
更新日期:2007-04-01 00:00:00
abstract::The vertebrate pore protein Nup153 plays pivotal roles in nuclear pore function. In addition to being important to pore architecture, Nup153 is a key participant in both import and export. The scope of Nup153 function also extends beyond the canonical view of the pore as a trafficking gateway. During the transition in...
journal_title:Chromosoma
pub_type: 杂志文章,评审
doi:10.1007/s00412-005-0019-3
更新日期:2005-11-01 00:00:00
abstract::When A-banded human chromosomes mounted in water are transferred to an organic mounting medium, the chromosomes show uniform bright quinacrine fluorescence. This change is reversible. It is inferred that quinacrine is bound uniformly along the chromosomes, and that Q-banding is a consequence of a non-uniform distribut...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00294941
更新日期:1977-12-06 00:00:00
abstract::Fluorescence in situ hybridization (FISH), especially chromosome painting, has been extensively exploited in the phylogenetic reconstruction of primate evolution. Although chromosome painting is a key method to map translocations, it is not effective in detecting chromosome inversions, which may be up to four times mo...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-015-0565-2
更新日期:2016-09-01 00:00:00
abstract::In addition to genetic information, mitotic chromosomes transmit essential components for nuclear assembly and function in a new cell cycle. A specialized chromosome domain, called the perichromosomal layer, perichromosomal sheath, chromosomal coat, or chromosome surface domain, contains proteins required for a variet...
journal_title:Chromosoma
pub_type: 杂志文章,评审
doi:10.1007/s00412-005-0021-9
更新日期:2005-12-01 00:00:00
abstract::Ribosomal DNA nontranscribed spacers in Drosophila virilis DNA have been examined in some detail by restriction site analysis of cloned segments of rDNA, nucleic acid hybridizations involving unfractionated rDNA, and base composition estimates. The overall G + C content of the spacer is 27--28%; this compares with 39%...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00285773
更新日期:1981-01-01 00:00:00
abstract::Macronuclear DNA was isolated from Paramecium primaurelia, stock 168. Although the macronucleus is polyploid to the extent of 840C, in other respect the DNA appears to be simply organized, having neither satellite sequences nor substantial amounts of intermediately repetitive sequence. The sequence complexity of macro...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00328389
更新日期:1980-01-01 00:00:00
abstract::Sugar beet (Beta vulgaris) chromosomes consist of large heterochromatic blocks in pericentromeric, centromeric, and intercalary regions comprised of two different highly abundant DNA satellite families. To investigate DNA methylation at single base resolution at heterochromatic regions, we applied a method for strand-...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-011-0325-x
更新日期:2011-08-01 00:00:00
abstract::In Drosophila, the Polycomb group and trithorax group proteins play a critical role in controlling the expression states of homeotic gene complexes during development. The common view is that these two classes of proteins bind to the homeotic complexes and regulate transcription at the level of chromatin. In the prese...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-007-0123-7
更新日期:2008-02-01 00:00:00
abstract::A simple method has been described for the visualization of chromosomes cores with light microscopy in conventional chromosome preparations. The technique is relatively simple, highly reproducible and can be used effectively on fresh and aged slides. The following observations have been made: (1) a core existed in mit...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00292418
更新日期:1980-01-01 00:00:00
abstract::Spermatogenic cells of the mouse have been separated by gravity sedimentation using a modification of a previously published method. Details are given for the collection of purified samples of specific meiotic stages and for the collection of labelled cell fractions following injection of 3H-thymidine. Suppression of ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00286046
更新日期:1977-07-08 00:00:00
abstract::The mean DNA content of G2 macronuclei varies during the life cycle of the ciliate Tetrahymena thermophila. Early in the life cycle the mean is about 130 C; later it is about 94 C. In hybrids between strains A and B the decrease from 130 C to 94 C usually began after 60 fissions after conjugation. In B X B clones the ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00327377
更新日期:1978-10-20 00:00:00
abstract::The putative nucleolus in Saccharomyces cerevisiae is visible in electron micrographs as a darkly stained, crescent-shaped structure associated with the nuclear envelope. The haploid yeast genome contains 100-200 tandem copies of a 9.1 kb ribosomal DNA (rDNA) repeat predicted to reside in this structure. We combined i...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00352202
更新日期:1991-09-01 00:00:00
abstract::In Drosophila gibberosa, differences between midgut and salivary gland chromosomes fall into two categories: tissue-specific band modulations which persist throughout the 90 h developmental period that we studied and tissue-specific puffs. Puffs that are common to both tissues tend to appear earlier in the midgut. Som...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00292969
更新日期:1988-11-01 00:00:00
abstract::By using the optical properties of birefringence of DNA, the arrangement of these molecules has been studied in Dinoflagellate chromosomes and Dipteran polytene chromosomes. These latter are used, here, as a reference material. These observations have been made under a polarizing microscope on intact and stretched chr...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00330371
更新日期:1978-08-21 00:00:00
abstract::The ribosomal DNA(rDNA)-containing chromatin in eukaryotes forms a unique architecture called the "secondary constriction" or "nucleolus organiser region (NOR)" on mitotic chromosomes. To gain more insight into non-histone chromosomal proteins (NHCP), termed "N-band proteins", that are specifically associated with the...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00292743
更新日期:1986-01-01 00:00:00
abstract::The antigens in HeLa and Trichosia pubescens cells, recognized by sera from patients with rheumatic diseases containing anti-Ku antibodies, were compared by means of immunoprecipitation of labeled cell extracts. The autoantibodies present in the tested sera precipitate at least two polypeptides of approximately Mr = 7...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF01735325
更新日期:1990-04-01 00:00:00
abstract::The reduction in chromosome number during meiosis is essential for the production of haploid germ cells and thereby fertility. To achieve this, homologous chromosomes are first synapsed together by a protein assembly, the synaptonemal complex (SC), which permits genetic exchange by crossing over and the subsequent acc...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-018-00688-z
更新日期:2019-09-01 00:00:00
abstract::We have used a new approach involving in situ hybridisation and electron microscopy to establish ultrastructural homologies between polytene chromosome regions of Drosophila melanogaster and Drosophila subobscura. Twelve probes were chosen to cover all the chromosomal elements: the myospheroid gene, the collagen type ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s004120050287
更新日期:1998-05-01 00:00:00
abstract::The fourth chromosome smallest in the genome of Drosophila melanogaster differs from other chromosomes in many ways. It has high repeat density in conditions of a large number of active genes. Gray bands represent a significant part of this polytene chromosome. Specific proteins including HP1a, POF, and dSETDB1 establ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-019-00703-x
更新日期:2019-06-01 00:00:00
abstract::To structurally dissect mitotic chromosomes, we aim to position along the folded chromatin fiber proteins involved in long-range order, such as topoisomerase IIalpha (topoIIalpha) and condensin. Immuno-electron microscopy (EM) of thin-sectioned chromosomes is the method of choice toward this goal. A much-improved immu...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-005-0023-7
更新日期:2005-11-01 00:00:00
abstract::The spatial distribution of parental genomes has attracted much interest because intranuclear chromosome distribution can modulate the transcriptome of cells and influence the efficacy of meiotic homologue pairing. Pairing of parental chromosomes is imperative to sexual reproduction as it translates into homologue seg...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-014-0479-4
更新日期:2014-12-01 00:00:00
abstract::Chromosome replication in the last premeiotic S-phase of male mammals has been previously studied by [3H]thymidine autoradiography and by a 5-bromodeoxyuridine (BrdU)/Giemsa technique. We used a recently developed BrdU-antibody technique (BAT) in this study. The following conclusions were drawn: (1) The replication pa...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00346006
更新日期:1992-03-01 00:00:00
abstract::The X and Y chromosomes of the musk shrew are the two largest in the complement and they regularly form a single chiasma during meiosis. This chiasma is located in the short arms of the X and Y, both of which show partial C-banding at meiosis. The in vitro incorporation of 5-bromodeoxyuridine/tritiated thymidine durin...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00327596
更新日期:1986-01-01 00:00:00
abstract::The haspins are divergent members of the eukaryotic protein kinase family that are conserved in many eukaryotic lineages including animals, fungi, and plants. Recently-solved crystal structures confirm that the kinase domain of human haspin has unusual structural features that stabilize a catalytically active conforma...
journal_title:Chromosoma
pub_type: 杂志文章,评审
doi:10.1007/s00412-009-0250-4
更新日期:2010-04-01 00:00:00
abstract::The 17 kDa human autoantigen designated CENP-A is a centromere specific histone. We show here that CENP-A is present in tissue of bovine origin, and that it is quantitatively retained in mature spermatozoa. This result is striking, as a prominent feature of spermatogenesis in mammals is the replacement of most somatic...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00337600
更新日期:1990-12-01 00:00:00