Abstract:
:The 17 kDa human autoantigen designated CENP-A is a centromere specific histone. We show here that CENP-A is present in tissue of bovine origin, and that it is quantitatively retained in mature spermatozoa. This result is striking, as a prominent feature of spermatogenesis in mammals is the replacement of most somatic and testes specific histones with protamines. Indirect immunofluorescence studies further show that CENP-A is retained in sperm nuclei in discrete foci, rather than being dispersed throughout the sperm head. These observations suggest that CENP-A is a functionally important component of centromeres, and that pre-existing CENP-A:DNA interactions are likely to be important in organizing the centromeres of the paternal genome during early embryogenesis.
journal_name
Chromosomajournal_title
Chromosomaauthors
Palmer DK,O'Day K,Margolis RLdoi
10.1007/BF00337600subject
Has Abstractpub_date
1990-12-01 00:00:00pages
32-6issue
1eissn
0009-5915issn
1432-0886journal_volume
100pub_type
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