A neocentromere in the DAZ region of the human Y chromosome.

abstract：:We describe a method of mapping genes or transcripts on polytene chromosomes by transmission electron microscopy. We present several applications which illustrate that, in favorable cases, the method has a resolution of ca. 10 kg, and that high resolution mapping of hybridization sites relative to bands and puffs can ...

journal_title：Chromosoma

authors： Kress H,Meyerowitz EM,Davidson N

更新日期：1985-01-01 00:00:00

abstract：:Lack of crossing-over in meiosis can trigger an apoptotic response at metaphase I by the spindle assembly checkpoint (SAC). In contrast to females, segregation of sex chromosomes in males poses a particular challenge as recombination and chiasma formation is restricted to the pseudoautosomal region, the small region o...

journal_title：Chromosoma

authors： Faisal I,Kauppi L

更新日期：2016-06-01 00:00:00

abstract：:In Drosophila, the Polycomb group and trithorax group proteins play a critical role in controlling the expression states of homeotic gene complexes during development. The common view is that these two classes of proteins bind to the homeotic complexes and regulate transcription at the level of chromatin. In the prese...

journal_title：Chromosoma

authors： Fanti L,Perrini B,Piacentini L,Berloco M,Marchetti E,Palumbo G,Pimpinelli S

更新日期：2008-02-01 00:00:00

abstract：:The nuclear topography of pericentromeric DNA of chromosome 11 was analyzed in G0 (nonstimulated) and G1 [phytohemagglutinin (PHA) stimulated] human lymphocytes by confocal microscopy. In addition to the nuclear center, the centrosome was used as a second point of reference in the three-dimensional (3D) analysis. Peri...

journal_title：Chromosoma

authors： Hulspas R,Houtsmuller AB,Krijtenburg PJ,Bauman JG,Nanninga N

更新日期：1994-07-01 00:00:00

abstract：:The 5S RNA gene content of polyploid cells of the ovary of Drosophila melanogaster has been compared in animals with two or three gene clusters. The amount of 5S RNA genes is exactly proportional to the number of gene clusters as determined by DNA-RNA filter hybridization. In contrast, the number of rDNA genes in endo...

journal_title：Chromosoma

authors： Renkawitz-Pohl R

更新日期：1979-01-31 00:00:00

abstract：:Nuclear and cytoplasmic RNA of Stylonychia mytilus were analyzed on denaturing polyacrylamide gels. The molecular weight of rRNA precursor molecules is within a range of 2.1 x 10(6) daltons. A comparison between the electrophoretic pattern of nuclear non-ribosomal RNA and cytoplasmic mRNA indicates that a considerable...

journal_title：Chromosoma

authors： Nock A

更新日期：1981-01-01 00:00:00

abstract：:Human lymphocytes were grown for one replication cycle in BrdU, stained with 33258 Hoechst, exposed to UV light and subsequently treated with 2 x SSC and stained with Giemsa. This technique differentially stains the constitutive heterochromatin of chromosomes 1, 9, 15, 16, and the Y. In the heterochromatin of chromoso...

journal_title：Chromosoma

authors： Angell RR,Jacobs PA

更新日期：1975-08-11 00:00:00

abstract：:Using BrdU/Hoechst 33258/Giemsa methods for detecting replicating chromosome bands, a method is described by which the DNA synthesis phase may be sub-divided on the basis of distinctive patterns displayed by certain chromosomes.--Applied to asynchronous populations successively sampled through one cell cycle, cells in...

journal_title：Chromosoma

authors： Savage JR,Bhunya SP

更新日期：1980-01-01 00:00:00

abstract：:In order to induce chromosomal rearrangements, males were exposed to x-rays and then mated to non-irradiated females. The number of each type of structural alteration was determined by examination of the polytene chromosomes of the F1 progeny. -- A comparison of the results with similar studies made on Drosophila reve...

journal_title：Chromosoma

authors： Israelewski N

更新日期：1975-12-10 00:00:00

abstract：:The DNA coding for 28 S and 18 S ribosomal RNA, including the spacer regions, has been isolated from calf (Bos taurus) thymus gland. The method used included shearing of the total DNA to a highly homogeneous size population, selective heat denaturation and S1 nuclease treatment to remove single stranded DNA. Repeated ...

journal_title：Chromosoma

authors： Blin N,Stephenson EC,Stafford DW

更新日期：1976-10-12 00:00:00

abstract：:The binding of methylene blue to DNA and chromatin treated in various ways was examined by equilibrium dialysis. The maximum r value (moles of bound dye/mole of nucleotide) was 1.0 for DNA, 0.6 for unfixed chromatin, and 0.83 for chromatin fixed in methanol-acetic acid. When fixed chromatin was treated with saline-cit...

journal_title：Chromosoma

authors： Comings DE,Avelino E

更新日期：1975-08-11 00:00:00

abstract：:When A-banded human chromosomes mounted in water are transferred to an organic mounting medium, the chromosomes show uniform bright quinacrine fluorescence. This change is reversible. It is inferred that quinacrine is bound uniformly along the chromosomes, and that Q-banding is a consequence of a non-uniform distribut...

journal_title：Chromosoma

authors： Sumner AT

更新日期：1977-12-06 00:00:00

abstract：:In a variety of organisms, euchromatic genes brought into juxtaposition with pericentric heterochromatin show position-effect variegation (PEV), a silencing of gene expression in a subset of the cells in which the gene is normally expressed. Previously, a P-element mobilization screen identified transgenic Drosophila ...

journal_title：Chromosoma

authors： Cryderman DE,Cuaycong MH,Elgin SC,Wallrath LL

更新日期：1998-11-01 00:00:00

abstract：:Meiotic pairing and segregation were studied in three different heterozygous reciprocal translocation strains of the baker's yeast, Saccharomyces cerevisiae. Pachytene translocation quadrivalents were identified by a combination of immunofluorescence and fluorescence in situ hybridization and the karyotypes of meiotic...

journal_title：Chromosoma

authors： Loidl J,Jin QW,Jantsch M

更新日期：1998-09-01 00:00:00

abstract：:Exposure of Nyctotherus ovalis to low temperatures or vinblastine caused similar reactions of "classes" of microtubules (mt) present in the mitotic micronucleus of this ciliate towards both treatments. However, differences of sensitivity between certain "classes" of mt at individual mitotic stages exist. Unlike the ki...

journal_title：Chromosoma

authors： Eichenlaub-Ritter U,Ruthmann A

更新日期：1982-01-01 00:00:00

abstract：:The order Monotremata, comprising the platypus and two species of echidna (Australian and Nuigini) is the only extant representative of the mammalian subclass Prototheria, which diverged from subclass Theria (marsupials and placental mammals) 150-200 million years ago. The 2n = 63 male, 64 female karyotype (newly desc...

journal_title：Chromosoma

authors： Wrigley JM,Graves JA

更新日期：1988-01-01 00:00:00

abstract：:The evolutionary conservation of Y chromosomal DNA sequences of Drosophila hydei in different species of the genus Drosophila was studied by in situ hybridization and on genomic DNA blots of restriction enzyme digested DNA. We demonstrated that Y specific DNA sequences, which form major parts of lampbrush loops relate...

journal_title：Chromosoma

authors： Vogt P,Hennig W,ten Hacken D,Verbost P

更新日期：1986-01-01 00:00:00

abstract：:In most multicellular eukaryotes, synapsis [synaptonemal complex (SC) formation] between pairs of homologous chromosomes during prophase I of meiosis is closely linked with crossing over. Asynaptic mutants in plants have reduced synapsis and increased univalent frequency, often resulting in genetically unbalanced game...

journal_title：Chromosoma

authors： Qiao H,Offenberg HH,Anderson LK

更新日期：2012-06-01 00:00:00

abstract：:The chironomid midge Pseudodiamesa branickii shows three polytene, banded elements in salivary gland and Malpighian tubule cells, none of which carry a nucleolar organizer. The present investigation revealed four chromosome pairs in mitotic metaphases, the nucleolus was localized in the tiny chromosome 4. Microphotome...

journal_title：Chromosoma

authors： Zacharias H

更新日期：1984-01-01 00:00:00

abstract：:Human neutrophil nuclei typically consist of three of four large heterochromatic lobes joined by thin, DNA-containing filaments. In addition, some lobes exhibit appendages of various sizes and shapes. Classical genetic and cytological studies suggest that some appendages contain specific chromosomes. The studies repor...

journal_title：Chromosoma

authors： Aquiles Sanchez J,Karni RJ,Wangh LJ

更新日期：1997-08-01 00:00:00

abstract：:The X and Y chromosomes of the musk shrew are the two largest in the complement and they regularly form a single chiasma during meiosis. This chiasma is located in the short arms of the X and Y, both of which show partial C-banding at meiosis. The in vitro incorporation of 5-bromodeoxyuridine/tritiated thymidine durin...

journal_title：Chromosoma

authors： Raman R,Nanda I

更新日期：1986-01-01 00:00:00

abstract：:Chromosome paints of the rat kangaroo ( Aepyprymnus rufuscens, 2 n=32) were used to define chromosome regions in the long nosed potoroo ( Potorous tridactylus, 2 n=12 female, 13 male) karyotype and localize these regions in three-dimensionally preserved nuclei of the potoroo to test the hypothesis that marsupial chrom...

journal_title：Chromosoma

authors： Rens W,O'Brien PC,Graves JA,Ferguson-Smith MA

更新日期：2003-08-01 00:00:00

abstract：:Engineered minichromosomes provide efficient platforms for stacking transgenes in crop plants. Methods for modifying these chromosomes in vivo are essential for the development of customizable systems for the removal of selection genes or other sequences and for the addition of new genes. Previous studies have demonst...

journal_title：Chromosoma

authors： Gaeta RT,Masonbrink RE,Zhao C,Sanyal A,Krishnaswamy L,Birchler JA

更新日期：2013-06-01 00:00:00

abstract：:Saccharomyces cerevisiae cells containing null mutations in the SEP1 gene, which encodes the homologous pairing and strand exchange protein p175SEP1, enter pachytene with a delay. They arrest uniformly at this stage of meiotic prophase, probably revealing a checkpoint in the transition from pachytene to meiosis I. At ...

journal_title：Chromosoma

authors： Bähler J,Hagens G,Holzinger G,Scherthan H,Heyer WD

更新日期：1994-04-01 00:00:00

abstract：:Membrane-free folded chromosomes were purified from log-phase cultures of Escherichia coli and prepared for electron microscopy by aqueous (Kleinschmidt and Zahn) spreading. The appearance of the chromosomes depended on the salt concentrations in spreading. At certain salt concentration, the chromosomes resembled rose...

journal_title：Chromosoma

authors： Kavenoff R,Bowen BC

更新日期：1976-12-16 00:00:00

abstract：:In addition to genetic information, mitotic chromosomes transmit essential components for nuclear assembly and function in a new cell cycle. A specialized chromosome domain, called the perichromosomal layer, perichromosomal sheath, chromosomal coat, or chromosome surface domain, contains proteins required for a variet...

journal_title：Chromosoma

authors： Van Hooser AA,Yuh P,Heald R

更新日期：2005-12-01 00:00:00

abstract：:Ki-67 protein has been widely used as a proliferation marker for human tumor cells for decades. In recent studies, multiple molecular functions of this large protein have become better understood. Ki-67 has roles in both interphase and mitotic cells, and its cellular distribution dramatically changes during cell cycle...

journal_title：Chromosoma

authors： Sun X,Kaufman PD

更新日期：2018-06-01 00:00:00

abstract：:The method of ultrathin sections of unsquashed salivary gland polytene chromosomes of Ch. thummi was applied to their ultrastrual mapping. There was a good agreement between electron micrographs and Hägele's light microscopic map (1970) with respect to the pattern and number of bands. 94% of bands were identified in l...

journal_title：Chromosoma

authors： Kiknadze II,Perov NA,Chentsov YS

更新日期：1976-03-31 00:00:00

abstract：:We provide evidence that centromere-specific 155 bp DNA repeats terminate one pair of telomeres at the telocentric, left end of the short fourth chromosome in Chironomus pallidivittatus. Earlier evidence indicated that all other telomeres are terminated by 340 bp telomere-specific repeats. DNA that borders the 155 bp ...

journal_title：Chromosoma

authors： Rosén M,Castillejo-López C,Edström JE

更新日期：2002-02-01 00:00:00

abstract：:The distribution of constitutive heterochromatin has been investigated in four chromosomal races of the grasshopper Caledia captiva (2n=23 male/24 female) by the C-banding technique. Each of the four races was found to have a distinctive banding pattern which is associated with the inter-racial differences in chromoso...

journal_title：Chromosoma

authors： Shaw DD,Webb GC,Wilkinson P

更新日期：1976-06-30 00:00:00