Abstract:
:Using electron spectroscopic imaging, a new type of small granular structural constituent has been observed in the extrachromosomal zone of the polytene nucleus of the salivary gland cells of Chironomus thummi and Chironomus tentans. These granules appear isolated or in small clumps and are often seen to be connected with surrounding thin fibrils. They are stained by the EDTA procedure, which is preferential for nuclear ribonucleoprotein (RNP) constituents, and by the bismuth oxynitrate method for visualizing phosphorylated compounds. The granules are 15-23 nm in diameter and are digested by prolonged post-embedding RNAse hydrolysis. These structural elements contain the highest concentration of phosphorus in the interchromosomal space as revealed by electron energy loss spectroscopy. The small granules exhibit several morphological and cytochemical features in common with interchromatin granules, but they are not labeled with antibodies directed against extranucleolar small nuclear RNPs (snRNPs), as are interchromatin granules.
journal_name
Chromosomajournal_title
Chromosomaauthors
Vázquez-Nin GH,Echeverría OM,Rouelle-Rossier VB,Fakan Sdoi
10.1007/BF00650895subject
Has Abstractpub_date
1993-12-01 00:00:00pages
693-9issue
10eissn
0009-5915issn
1432-0886journal_volume
102pub_type
杂志文章相关文献
CHROMOSOMA文献大全abstract::During DNA synthesis there is a distinct stage immediately after the joining of large DNA replication intermediates (post-elongation stage). The conversion of this DNA to mature DNA was analysed in cells treated with aphidicolin to stop the movement of the replication fork. In such cells mature DNA is formed. In contr...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00330347
更新日期:1987-01-01 00:00:00
abstract::At meiotic prophase the chromatin becomes arranged in loops on newly formed chromosome cores. The cores of homologous chromosomes become aligned in parallel and thus form the synaptonemal complex (SC), a structure found in the meiocytes of nearly all recombinationally competent, sexually reproducing organisms. We repo...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00292383
更新日期:1989-11-01 00:00:00
abstract::Previous studies of the Robertsonian polymorphism in the Atlantic dog-whelk, Nucella lapillus (2n = 26-36), have been limited by the inability to identify unequivocally individual chromosomes in the karyotype. This species, as with many other marine invertebrates, has proven largely refractory to the standard (mammali...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00352269
更新日期:1996-03-01 00:00:00
abstract::In this study, a new chromosome fluorescence banding technique was developed in plants. The technique combined 4',6-diamidino-2-phenylindole (DAPI) staining with software analysis including three-dimensional imaging after deconvolution. Clear multiple and adjacent DAPI bands like G-bands were obtained by this techniqu...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-004-0289-1
更新日期:2004-08-01 00:00:00
abstract::A crucial step during eukaryotic initiation of DNA replication is the correct loading and activation of the replicative DNA helicase, which ensures that each replication origin fires only once. Unregulated DNA helicase loading and activation, as it occurs in cancer, can cause severe DNA damage and genomic instability....
journal_title:Chromosoma
pub_type: 杂志文章,评审
doi:10.1007/s00412-014-0489-2
更新日期:2015-03-01 00:00:00
abstract::A karyotype based on banding pattern and chromosome length is presented for the white-handed gibbon, Hylobates lar. Little homology with the banding patterns of the chromosomes of the other Hominoidea can be seen, confirming the early evolutionary separation of Hylobatidae and the other apes. Hybridization in situ wit...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00285805
更新日期:1975-01-01 00:00:00
abstract::High resolution patterns of DNA replications in human lymphocyte chromosomes during early and late S-phases were studied by means of the BrdU-Hoechst-Giemsa technique. The late replicating bands were found to be identical with highly detailed G-bands. Between early replicating bands and R-bands subtile differences wer...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00286114
更新日期:1981-01-01 00:00:00
abstract::While many studies have provided significant insight into homolog pairing during meiosis, information on non-homologous pairing is much less abundant. In the present study, fluorescence in situ hybridization (FISH) was used to investigate non-homologous pairing in haploid rice during meiosis. At pachytene, non-homolog...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-010-0288-3
更新日期:2011-02-01 00:00:00
abstract::Ki-67 protein has been widely used as a proliferation marker for human tumor cells for decades. In recent studies, multiple molecular functions of this large protein have become better understood. Ki-67 has roles in both interphase and mitotic cells, and its cellular distribution dramatically changes during cell cycle...
journal_title:Chromosoma
pub_type: 杂志文章,评审
doi:10.1007/s00412-018-0659-8
更新日期:2018-06-01 00:00:00
abstract::A plant carrying a small extra chromosome was found in Landsberg erecta ecotype of Arabidopsis thaliana. Fluorescence in situ hybridization revealed that this minichromosome was derived from the short arm of chromosome 4. The size of this "mini4S" chromosome was estimated to be approximately 7.5 Mb on the basis of pre...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-005-0045-1
更新日期:2006-08-01 00:00:00
abstract::With simultaneous immunofluorescence and fluorescent in situ hybridization, we have determined the organization of native and heterologous DNA sequences relative to the cores of meiotic prophase chromosomes. The normal chromatin organization is demonstrated with probes of mouse sequences: a cosmid probe that identifie...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00362284
更新日期:1994-10-01 00:00:00
abstract::In addition to genetic information, mitotic chromosomes transmit essential components for nuclear assembly and function in a new cell cycle. A specialized chromosome domain, called the perichromosomal layer, perichromosomal sheath, chromosomal coat, or chromosome surface domain, contains proteins required for a variet...
journal_title:Chromosoma
pub_type: 杂志文章,评审
doi:10.1007/s00412-005-0021-9
更新日期:2005-12-01 00:00:00
abstract::The genus Phaseolus L. has been subject of extensive cytogenetic studies due to its global economic importance. It is considered karyotypically stable, with most of its ca. 75 species having 2n = 22 chromosomes, and only three species (Phaseolus leptostachyus, Phaseolus macvaughii, and Phaseolus micranthus), which for...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-015-0548-3
更新日期:2016-06-01 00:00:00
abstract::Using indirect immunofluorescence visualization techniques we investigated the distribution of RNA polymerase B (or II) and histone H1 at heat shock puff loci in Drosophila melanogaster polytene chromosomes at different times during and after heat shock. After heat treatments of from 5 to 45 min, the heat shock puff d...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00329465
更新日期:1978-01-16 00:00:00
abstract::The chironomid midge Pseudodiamesa branickii shows three polytene, banded elements in salivary gland and Malpighian tubule cells, none of which carry a nucleolar organizer. The present investigation revealed four chromosome pairs in mitotic metaphases, the nucleolus was localized in the tiny chromosome 4. Microphotome...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00292473
更新日期:1984-01-01 00:00:00
abstract::Ribosomal DNA (rDNA) variation in the species Triturus vulgaris meridionalis (Amphibia, Urodela) is remarkable because of unusually high intraspecific variability in the number and distribution of ribosomal loci in the karyotype; in addition, portions of the intergenic spacer (IGS) are clustered at chromosomal loci wh...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s004120050253
更新日期:1997-10-01 00:00:00
abstract::Mutation of the telomeric repeat sequence has severe cellular consequences in a variety of systems. A Tetrahymena thermophila telomerase template mutant, ter1-43AA, displays an acute mitotic chromosome segregation defect. In the study described here we investigated the molecular basis for this lethality. Although clon...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-003-0233-9
更新日期:2003-04-01 00:00:00
abstract::In 1906, the Spanish neurobiologist Santiago Ramón y Cajal was awarded the Nobel Prize in Physiology or Medicine in recognition of his work on the structure of neurons and their connections. Cajal is commonly regarded as the father of modern neuroscience. What is less well known is that Cajal also had a great interest...
journal_title:Chromosoma
pub_type: 传,历史文章,杂志文章,评审
doi:10.1007/s00412-009-0212-x
更新日期:2009-08-01 00:00:00
abstract::The nuclear topography of pericentromeric DNA of chromosome 11 was analyzed in G0 (nonstimulated) and G1 [phytohemagglutinin (PHA) stimulated] human lymphocytes by confocal microscopy. In addition to the nuclear center, the centrosome was used as a second point of reference in the three-dimensional (3D) analysis. Peri...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00352253
更新日期:1994-07-01 00:00:00
abstract::We have used a new approach involving in situ hybridisation and electron microscopy to establish ultrastructural homologies between polytene chromosome regions of Drosophila melanogaster and Drosophila subobscura. Twelve probes were chosen to cover all the chromosomal elements: the myospheroid gene, the collagen type ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s004120050287
更新日期:1998-05-01 00:00:00
abstract::The time sequence of DNA replication in partially synchronized human amniotic fluid cells has been analysed, employing BrdU incorporation techniques.--Regardless of the interval between removal of the methotrexate/uridine block and addition of BrdU during S-phase, the treatment results in an R-type replication pattern...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00293176
更新日期:1978-06-28 00:00:00
abstract::Exposure of Nyctotherus ovalis to low temperatures or vinblastine caused similar reactions of "classes" of microtubules (mt) present in the mitotic micronucleus of this ciliate towards both treatments. However, differences of sensitivity between certain "classes" of mt at individual mitotic stages exist. Unlike the ki...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00330781
更新日期:1982-01-01 00:00:00
abstract::In Xenopus laevis eight tRNA genes are located in a 3.18 kb tandemly repeated unit. There are 150 copies of the unit at a single locus near the long arm telomere of one of the acrocentric chromosomes in the 14-17 group. Two additional classes of tRNA gene-containing repeats have been isolated (defined by clones p3.1 a...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00352227
更新日期:1995-10-01 00:00:00
abstract::Alterations in the nuclear positioning of chromosomes and specific genes during differentiation and development have suggested strongly the existence of a relationship between non-random organization of the genome and its function. In this study, we have examined the genome organization in interphase nuclei during adi...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-009-0225-5
更新日期:2009-10-01 00:00:00
abstract::Lack of crossing-over in meiosis can trigger an apoptotic response at metaphase I by the spindle assembly checkpoint (SAC). In contrast to females, segregation of sex chromosomes in males poses a particular challenge as recombination and chiasma formation is restricted to the pseudoautosomal region, the small region o...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-015-0542-9
更新日期:2016-06-01 00:00:00
abstract::Retroviruses have the ability to integrate into the genome of their host, in many cases with little apparent sequence or site specificity. However, relatively few studies have addressed more general features of chromosomal integration. In this study we directly visualized the chromosomal organization of three represen...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00355364
更新日期:1991-12-01 00:00:00
abstract::The X and Y chromosomes of the musk shrew are the two largest in the complement and they regularly form a single chiasma during meiosis. This chiasma is located in the short arms of the X and Y, both of which show partial C-banding at meiosis. The in vitro incorporation of 5-bromodeoxyuridine/tritiated thymidine durin...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00327596
更新日期:1986-01-01 00:00:00
abstract::In budding yeast, mitotic DNA replication initiates at sequence-specific replication origins, the prototype for which is ARS1. Initiation serves as the primary control point for mitotic DNA replication, and is catalyzed by the Cdc7 protein kinase. In contrast, premeiotic DNA replication apparently does not require Cdc...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00360406
更新日期:1993-06-01 00:00:00
abstract::Position-effect variegation (PEV) is the mosaic expression of a euchromatic gene brought into juxtaposition with heterochromatin. Fourteen different transformed Drosophila melanogaster lines with variegating P-element inserts were used to examine the DNA levels of these transgenes. Insert sites include pericentric, te...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00352116
更新日期:1996-04-01 00:00:00
abstract::After selective enrichment and differential hybridisation of Cot-1 DNA fractions of plants with and without polymorphic heterochromatic segments, a repetitive sequence (called Bds1) specific to the polymorphic chromosome segments of Brachycome dichromosomatica (Brachyscome dichromosomatica) was isolated. A single repe...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s004120050430
更新日期:2000-06-01 00:00:00