Abstract:
:During DNA synthesis there is a distinct stage immediately after the joining of large DNA replication intermediates (post-elongation stage). The conversion of this DNA to mature DNA was analysed in cells treated with aphidicolin to stop the movement of the replication fork. In such cells mature DNA is formed. In contrast, UV-A, which induces a wide spectrum of DNA lesions, inhibits the conversion to mature DNA. The data indicate that the maturation of the post-elongation stage can be uncoupled from the movement of the replication fork.
journal_name
Chromosomajournal_title
Chromosomaauthors
Lönn U,Lönn Sdoi
10.1007/BF00330347subject
Has Abstractpub_date
1987-01-01 00:00:00pages
171-4issue
3eissn
0009-5915issn
1432-0886journal_volume
95pub_type
杂志文章相关文献
CHROMOSOMA文献大全abstract::The antigens in HeLa and Trichosia pubescens cells, recognized by sera from patients with rheumatic diseases containing anti-Ku antibodies, were compared by means of immunoprecipitation of labeled cell extracts. The autoantibodies present in the tested sera precipitate at least two polypeptides of approximately Mr = 7...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF01735325
更新日期:1990-04-01 00:00:00
abstract::Cytogenetic analysis indicates that Balbiani Ring 2 (BR 2) in the two sibling species Chironomus tentans and Chironomus pallidivittatus arises from identifically banded segments in the salivary gland polytene chromosomes, although chromosomal rearrangements have occurred. In situ hybridization of BR 2 RNA to the polyt...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00285806
更新日期:1975-01-01 00:00:00
abstract::The heterochromatin of chromosomes 2 and 3 of Drosophila melanogaster contains about 30 essential genes defined by genetic analysis. In the last decade only a few of these genes have been molecularly characterized and found to correspond to protein-coding genes involved in important cellular functions. Moreover, sever...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-003-0241-9
更新日期:2003-07-01 00:00:00
abstract::Aedes aegypti spermatocytes were reconstructed from electron micrographs. The species has tight somatic pairing of the chromosomes, and there are therefore no classical leptotene and zygotene stages, but rather a gradual transition from somatic pairing to meiotic pairing (= pachytene). The term "prepachytene" has been...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00329808
更新日期:1985-01-01 00:00:00
abstract::Human satellite DNAs I, II and IV were transcribed to yield radioactive complementary RNAs (cRNAs). These cRNAs were hybridised to metaphase chromosomes of man, chimpanzee (Pan troglodytes), gorilla (Gorilla gorilla) and orang utan (Pongo pygmaeus). The results of this in situ hybridisation were analysed quantitativel...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00327453
更新日期:1977-09-27 00:00:00
abstract::We have studied the diameter of chromatin fibres embedded in epoxy resins for three different materials: mouse thymus, chicken erythrocytes and sea cucumber spermatozoa. We confirm that the diameter of chromatin fibres increases with linker length, both values being influenced by the protein composition of chromatin. ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00293338
更新日期:1989-06-01 00:00:00
abstract::Mutations in the MCPH1 gene result in primary microcephaly in combination with a unique cellular phenotype of defective chromosome condensation. MCPH1 patient cells display premature chromosome condensation in G2 phase of the cell cycle and delayed decondensation in early G1 phase, observable as an increased proportio...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-015-0512-2
更新日期:2015-12-01 00:00:00
abstract::Rice (Oryza sativa L.) centromeres are composed of 155-bp satellite repeats (CentO), centromere-specific retrotransposon (CRR), and a variety of other repeats. Previous studies have shown that CentO and CRR elements are both parts of the functional centromere/kinetochore complex. In this study, a naturally occurring k...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-013-0413-1
更新日期:2013-08-01 00:00:00
abstract::Nucleosome assembly involves deposition of a heterotetramer of histones H3/H4 onto DNA followed by two heterodimers of histones H2A/H2B. Cycles of nucleosome assembly and disassembly are essential to cellular events such as replication, transcription, and DNA repair. After synthesis in the cytoplasm, histones are shut...
journal_title:Chromosoma
pub_type: 杂志文章,评审
doi:10.1007/s00412-006-0087-z
更新日期:2007-04-01 00:00:00
abstract::Changes in the nuclear positioning of specific genes, depending on their expression status, have been observed in a large diversity of physiological processes. However, gene position is poorly documented for immune cells which have been subjected to activation following bacterial infection. Using a pig model, we focus...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-011-0328-7
更新日期:2011-10-01 00:00:00
abstract::Simple repeated GATA and GACA sequences which were originally isolated from sex-specific snake satellite DNA have been found subsequently in all eukaryotes studied. The organization of these sequences within the mouse genome was investigated here by using synthetic oligonucleotide probes as a novel tool in comparison ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00386791
更新日期:1986-01-01 00:00:00
abstract::Chromosome segregation in primary spermatocytes of the crane fly Nephrotoma suturalis was studied after exposure to Colcemid at doses that did not completely inhibit spindle formation. Colcemid was added either to the medium in which larvae were cultured or to Tricine buffer in which isolated testes were incubated. Pa...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00291004
更新日期:1985-01-01 00:00:00
abstract::The ability to reproduce is a major trait of living organisms. This ability is carried out by specialized reproductive cells-gametes. In mammals, gametes develop through a unique developmental pathway. Extensive changes in the epigenome of gametes occur during embryonic development. With birth, gametes continue to mat...
journal_title:Chromosoma
pub_type: 杂志文章,评审
doi:10.1007/s00412-019-00704-w
更新日期:2019-09-01 00:00:00
abstract::A new method is described which allows the purification of large quantities of nuclei (germinal vesicles) from Xenopus laevis vitellogenic oocytes of different developmental stages. From the isolated nuclei, purified nucleoli were obtained. The isolated germinal vesicles are transcriptionally active showing endogenous...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00328531
更新日期:1978-05-16 00:00:00
abstract::The salivary chromosomes of the l(3)tl mutant of D. melanogaster are considerably thicker and shorter than those of normal larvae. In most nuclei, chromosome shortening is associated with morphological changes of two types. a) The bands lose their distinctive pattern and become loose. The chromosome appears as a granu...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF01798344
更新日期:1976-04-21 00:00:00
abstract::At least two types of Robertsonian exchange are now known in the acrocentric chromosomes of man. Both types involve breakage in the arms adjacent to the centromere. Evidence is presented for a third type of exchange, one involving breakage within the centromere itself, in the grasshopper Percassa rugifrons. In this sp...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00326262
更新日期:1975-09-26 00:00:00
abstract::Three new 18S.26S rRNA gene loci were identified in common wheat by sequential N-banding and in situ hybridization (ISH) analysis. Locus Nor-A7 is located at the terminal area of the long arm of 5A in both diploid and polyploid wheats. Locus Nor-B6 is located in N-band 1BL2.5 of the long arm of chromosome 1B in Tritic...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00368010
更新日期:1994-06-01 00:00:00
abstract::Compelling evidence has been obtained in favour of the idea that the nuclear surface of higher plant cells is a microtubule-nucleating and/or organizing site (MTOC), in the absence of defined centrosomes. How these plant MTOC proteins are redistributed and function during the progression of the cell cycle remains enti...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00417882
更新日期:1994-09-01 00:00:00
abstract::To understand how microtubules interact in forming the mitotic apparatus and orienting and moving chromosomes, the precise arrangement of microtubules in kinetochore fibers in Chinese hamster ovary cells was examined. Individual microtubules were traced, using high voltage electron microscopy of serial 0.25 micron sec...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00328277
更新日期:1981-01-01 00:00:00
abstract::This investigation was conducted to determine the relationship between Y chromosome loss and increased micronucleus formation with age. We also investigated the status of kinetochore proteins in the micronuclei. Umbilical cord blood samples were obtained from 18 newborn males, and peripheral blood was obtained from 35...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00344234
更新日期:1995-07-01 00:00:00
abstract::The fourth chromosome smallest in the genome of Drosophila melanogaster differs from other chromosomes in many ways. It has high repeat density in conditions of a large number of active genes. Gray bands represent a significant part of this polytene chromosome. Specific proteins including HP1a, POF, and dSETDB1 establ...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-019-00703-x
更新日期:2019-06-01 00:00:00
abstract::By means of a cytological technique involving 5-bromodeoxyuridine, acridine orange, and fluorescence microscopy, the asynchronously replicating, hence genetically inactivated, X chromosome was identified in 6- to 8-day embryos from female mice heterozygous for Searle's translocation T(X;16)16H (abbreviated as T16H) ma...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00368155
更新日期:1980-01-01 00:00:00
abstract::A higher order organization of the centromeres in the form of clustering of these DNA loci has been observed in many organisms. While centromere clustering is biologically significant to achieve faithful chromosome segregation, the underlying molecular mechanism is yet to be fully understood. In budding yeast, a kinet...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-019-00694-9
更新日期:2019-06-01 00:00:00
abstract::The chironomid midge Pseudodiamesa branickii shows three polytene, banded elements in salivary gland and Malpighian tubule cells, none of which carry a nucleolar organizer. The present investigation revealed four chromosome pairs in mitotic metaphases, the nucleolus was localized in the tiny chromosome 4. Microphotome...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00292473
更新日期:1984-01-01 00:00:00
abstract::The members of the serine-arginine (SR) family of proteins play multiple roles in posttranscriptional gene expression. Initially considered as essential splicing factors confined to the nucleus and regulating constitutive and alternative splicing, SR proteins are now known to shuttle between the nucleus and the cytopl...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s00412-006-0073-5
更新日期:2006-12-01 00:00:00
abstract::We sought for cloned sequences of middle repetitive (MR) complexity that mark obligatory heterochromatic regions. Total genome probes were employed in a differential screening procedure to recover X-specific, Y-specific and autosomal specific heterochromatic sequences. X-and Y-linked sequences were recovered in the sa...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00330119
更新日期:1982-01-01 00:00:00
abstract::The evolutionary conservation of Y chromosomal DNA sequences of Drosophila hydei in different species of the genus Drosophila was studied by in situ hybridization and on genomic DNA blots of restriction enzyme digested DNA. We demonstrated that Y specific DNA sequences, which form major parts of lampbrush loops relate...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00328637
更新日期:1986-01-01 00:00:00
abstract::The distribution of quinacrine and protein sulphur has been compared with that of DNA in euchromatic and heterochromatic regions of mouse chromosomes stained with the fluorescent dye quinacrine, using X-ray microanalysis. Heterochromatin tends to bind relatively more quinacrine than euchromatin, and contains a greater...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00294059
更新日期:1985-01-01 00:00:00
abstract::In this work we have used the inhibitor of F-actin polymerisation cytochalasin B (Cyt B) to test the hypothesis that the contractile ring and the central spindle are mutually interdependent structures in mammalian mitotic cells. Double fluorescence staining of alpha-tubulin and F-actin was employed to analyse anaphase...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/s004120050332
更新日期:1998-12-01 00:00:00
abstract::Isolated nuclei of the unicellular alga Olisthodiscus luteus, the uninucleate dinoflagellate Crypthecodinium cohnii and the binucleate dinoflagellate Peridinium balticum were lysed and deposited on grids by the microcentrifugation technique. The ultrastructure of the released chromatin fibers was compared to that of m...
journal_title:Chromosoma
pub_type: 杂志文章
doi:10.1007/BF00292229
更新日期:1980-01-01 00:00:00