FISH analysis of Drosophila melanogaster heterochromatin using BACs and P elements.

abstract：:The eukaryotic genome can be roughly divided into euchromatin and heterochromatin domains that are structurally and functionally distinct. Heterochromatin is characterized by its high compaction that impedes DNA transactions such as gene transcription, replication, or recombination. Beyond its role in regulating DNA a...

journal_title：Chromosoma

authors： Nikolov I,Taddei A

更新日期：2016-06-01 00:00:00

abstract：:BrdU-33258 Hoechst techniques have been used to characterize DNA replication patterns in lymphocytes from hunam females with supernumerary or structurally abnormal X chromosomes. Fluorescence analysis permits identification of late replicating X chromosomes in a very high proportion of cells and affords a high resolut...

journal_title：Chromosoma

authors： Latt SA,Willard HF,Gerald PS

更新日期：1976-08-17 00:00:00

abstract：:Centromere protein B (CENP-B) is a centromeric DNA-binding protein that binds to alpha-satellite DNA at the 17 bp CENP-B box sequence. The binding of CENP-B, along with other proteins, to alpha-satellite DNA sequences at the centromere, is thought to package the DNA into heterochromatin subjacent to the kinetochore of...

journal_title：Chromosoma

authors： Kapoor M,Montes de Oca Luna R,Liu G,Lozano G,Cummings C,Mancini M,Ouspenski I,Brinkley BR,May GS

更新日期：1998-12-01 00:00:00

abstract：:We determined the structure of the Schizosaccharomyces pombe centromere cen3 using direct genomic mapping and cosmid walking. The repetitive region of cen3 is approximately 110 kb, much longer than that of the previously determined cen1 and cen2 regions. The approximately 30 kb long left and approximately 60 kb right ...

journal_title：Chromosoma

authors： Murakami S,Matsumoto T,Niwa O,Yanagida M

更新日期：1991-12-01 00:00:00

abstract：:Macronuclear DNA was isolated from purified macronuclei of Paramecium aurelia and the size distribution was determined with regard to growth phase and method of extraction. DNA molecules as long as 105 microns and as short as 0.2 microns were observed. It was concluded that the method of extraction affected the observ...

journal_title：Chromosoma

authors： Cummings DJ

更新日期：1975-12-10 00:00:00

abstract：:The chromosomes of lake trout (Salvelinus namaycush) contain a considerable amount of heterochromatin located at the centromeres and/or telomeres of several chromosomes, including a sex-specific block located distally on the X chromosome. In order to investigate further the repetitive DNAs of lake trout, genomic DNA f...

journal_title：Chromosoma

authors： Reed KM,Phillips RB

更新日期：1995-12-01 00:00:00

abstract：:Nuclear and cytoplasmic RNA of Stylonychia mytilus were analyzed on denaturing polyacrylamide gels. The molecular weight of rRNA precursor molecules is within a range of 2.1 x 10(6) daltons. A comparison between the electrophoretic pattern of nuclear non-ribosomal RNA and cytoplasmic mRNA indicates that a considerable...

journal_title：Chromosoma

authors： Nock A

更新日期：1981-01-01 00:00:00

abstract：:The divergence of lineages leading to extant squamate reptiles (lizards, snakes, and amphisbaenians) and birds occurred about 275 million years ago. Birds, unlike squamates, have karyotypes that are typified by the presence of a number of very small chromosomes. Hence, a number of chromosome rearrangements might be ex...

journal_title：Chromosoma

authors： Pokorná M,Giovannotti M,Kratochvíl L,Kasai F,Trifonov VA,O'Brien PC,Caputo V,Olmo E,Ferguson-Smith MA,Rens W

更新日期：2011-10-01 00:00:00

abstract：:Cytogenetic analysis indicates that Balbiani Ring 2 (BR 2) in the two sibling species Chironomus tentans and Chironomus pallidivittatus arises from identifically banded segments in the salivary gland polytene chromosomes, although chromosomal rearrangements have occurred. In situ hybridization of BR 2 RNA to the polyt...

journal_title：Chromosoma

authors： Lambert B,Beermann W

更新日期：1975-01-01 00:00:00

abstract：:A cytological procedure for in vitro chromosome delineation has been studied using human and mouse (Mus musculus) chromosomes. This method, consisting of slide incubation in KMnO4 at 0--5 degrees C for 24 h followed by a short exposure to NaHSO3 (1--3 min) and Giemsa staining, induces extraction of chromatin from huma...

journal_title：Chromosoma

authors： Drets ME,Novello A

更新日期：1980-01-01 00:00:00

abstract：:The genomic DNA organisation patterns of four sauropsidian species, namely Python reticularis, Caiman crocodilus, Terrapene carolina triungius and Columba livia domestica were investigated by reassociation of short and long DNA fragments, by hyperchromicity measurements of reannealed fragments and by length estimation...

journal_title：Chromosoma

authors： Epplen JT,Diedrich U,Wagenmann M,Schmidtke J,Engel W

更新日期：1979-11-01 00:00:00

abstract：:MicroRNAs (miRNAs) were first discovered in genetic screens for regulators of developmental timing in the stem-cell-like seam cell lineage in Caenorhabditis elegans. As members of the heterochronic pathway, the lin-4 and let-7 miRNAs are required in the seam cells for the correct progression of stage-specific events a...

journal_title：Chromosoma

authors： Nimmo RA,Slack FJ

更新日期：2009-08-01 00:00:00

abstract：:Neuroblast chromosomes of 16 Drosophila melanogaster laboratory stocks (15 wild type and 1 carrying the mutant vermillion) were carefully analyzed for Q-banding patterns and morphological characteristics, in all the mitotic phases. Two forms of intraspecific heterochromatin variations, involving three types of chromos...

journal_title：Chromosoma

authors： Halfer C

更新日期：1981-01-01 00:00:00

abstract：:The hsp70, hsp83, hsromega, and the small heat shock protein genes were mapped on the polytene chromosomes of six species, representative of the geographical distribution of the Drosophila montium subgroup of the melanogaster species group. In addition, based on hybridization conditions, the putative locus of the hsp6...

journal_title：Chromosoma

authors： Drosopoulou E,Konstantopoulou I,Scouras ZG

更新日期：1996-08-01 00:00:00

abstract：:We describe a method of mapping genes or transcripts on polytene chromosomes by transmission electron microscopy. We present several applications which illustrate that, in favorable cases, the method has a resolution of ca. 10 kg, and that high resolution mapping of hybridization sites relative to bands and puffs can ...

journal_title：Chromosoma

authors： Kress H,Meyerowitz EM,Davidson N

更新日期：1985-01-01 00:00:00

abstract：:Short DNA regions, known to contain replication origins, were isolated from 2 M NaCl resistant nuclear structures of Physarum polycephalum after predigestion with DNase. Regions of 100 bp average length were cloned and sequenced. About 25% of the clones contained direct repeats of 12 to 16 bp and variable base sequenc...

journal_title：Chromosoma

authors： Opstelten RJ,Clement JM,Wanka F

更新日期：1989-12-01 00:00:00

abstract：:The preferred positions for meiotic double-strand breakage were mapped on Saccharomyces cerevisiae chromosomes I and VI, and on a number of yeast artificial chromosomes carrying human DNA inserts. Each chromosome had strong and weak double-strand break (DSB) sites. On average one DSB-prone region was detected by pulse...

journal_title：Chromosoma

authors： Klein S,Zenvirth D,Dror V,Barton AB,Kaback DB,Simchen G

更新日期：1996-12-01 00:00:00

abstract：:Ring chromosomes are of basic interest to the geneticist and cell biologist who study their behavior in meiotic and mitotic divisions. In addition, the mitotic instability associated with some ring-X chromosomes has proven useful in Drosophila as a means to produce gynandromorphs for developmental studies. We describe...

journal_title：Chromosoma

authors： Golic MM,Golic KG

更新日期：2011-04-01 00:00:00

abstract：:Diverse studies indicate that certain nonhistone proteins may be responsible for maintaining the integrity of the eukaryotic chromosomes. In view of their dispersal during nuclear division and their acknowledged role in gene expression, none of the nuclear matrix proteins can be regarded as integral proteins of the ch...

journal_title：Chromosoma

authors： De DN

更新日期：2002-07-01 00:00:00

abstract：:DNA double-strand breaks arise accidentally upon exposure of DNA to radiation and chemicals or result from faulty DNA metabolic processes. DNA breaks can also be introduced in a programmed manner, such as during the maturation of the immune system, meiosis, or cancer chemo- or radiotherapy. Cells have developed a vari...

journal_title：Chromosoma

authors： Ranjha L,Howard SM,Cejka P

更新日期：2018-06-01 00:00:00

abstract：:The ribosomal DNA(rDNA)-containing chromatin in eukaryotes forms a unique architecture called the "secondary constriction" or "nucleolus organiser region (NOR)" on mitotic chromosomes. To gain more insight into non-histone chromosomal proteins (NHCP), termed "N-band proteins", that are specifically associated with the...

journal_title：Chromosoma

authors： Matsui S,Fuke M,Chai L,Sandberg AA,Elassouli S

更新日期：1986-01-01 00:00:00

abstract：:The putative nucleolus in Saccharomyces cerevisiae is visible in electron micrographs as a darkly stained, crescent-shaped structure associated with the nuclear envelope. The haploid yeast genome contains 100-200 tandem copies of a 9.1 kb ribosomal DNA (rDNA) repeat predicted to reside in this structure. We combined i...

journal_title：Chromosoma

authors： Dvorkin N,Clark MW,Hamkalo BA

更新日期：1991-09-01 00:00:00

abstract：:The 1C DNA-content of isolated nuclei of postembryonic stages of Tetranychus urticae stained with the classic and a modified Schiff's reagent was cytophotometrically estimated as 0.1 pg, a low value in animals. For many tissues of this arrhenotokous species the ploidy ratio between males and females is 1:2, indicating...

journal_title：Chromosoma

authors： Tempelaar MJ

更新日期：1980-01-01 00:00:00

abstract：:The immunodeficiency, centromeric region instability, facial anomalies (ICF) syndrome is a rare autosomal recessive disease. Usually, it is caused by mutations in the DNA methyltransferase 3B gene, which result in decreased methylation of satellite DNA in the juxtacentromeric heterochromatin at 1qh, 16qh, and 9qh. Sat...

journal_title：Chromosoma

authors： Gisselsson D,Shao C,Tuck-Muller CM,Sogorovic S,Pålsson E,Smeets D,Ehrlich M

更新日期：2005-07-01 00:00:00

abstract：:The Indian muntjac (Muntiacus muntjak vaginalis) has a karyotype of 2n=6 in the female and 7 in the male, the karyotypic evolution of which through extensive tandem fusions and several centric fusions has been well-documented by recent molecular cytogenetic studies. In an attempt to define the fusion orientations of c...

journal_title：Chromosoma

authors： Chi JX,Huang L,Nie W,Wang J,Su B,Yang F

更新日期：2005-08-01 00:00:00

abstract：:Normal lepidopteran males produce two kinds of spermatozoa: nucleate (eupyrene) and anucleate (apyrene). Eupyrene spermatozoa have the usual type of elongate nuclei. But in apyrene spermatids, the nuclei never elongate and the chromatin remains in a telophase-like condition until enucleation occurs. The study of the d...

journal_title：Chromosoma

authors： Friedländer M,Hauschteck-Jungen E

更新日期：1982-01-01 00:00:00

abstract：:Studies using human and mouse cells have revealed some changes to non-telomeric chromatin and gene expression in response to abnormally short telomeres. To investigate this further, we studied the effect of inheriting shorter telomeres on transcription and genetic stability at non-telomeric sites in the mouse. Using m...

journal_title：Chromosoma

authors： Roberts AR,Huang E,Jones L,Daxinger L,Chong S,Whitelaw E

更新日期：2013-12-01 00:00:00

abstract：:An unstable minichromosome comprising part of the short arm of chromosome 10 of maize was recovered from an oil yellow variegated plant as a consequence of gamma irradiation of pollen. The cytological and gene dosage observations are consistent with the minichromosome being a partial isochromosome, which lags at mitot...

journal_title：Chromosoma

authors： Brock RD,Pryor AJ

更新日期：1996-06-01 00:00:00

abstract：:Targeted insertion of large pieces of DNA is an important goal of genetic engineering. However, this goal has been elusive since classical methods for homology-directed repair are inefficient and often not feasible in many systems. Recent advances are described here that enable site-specific genomic insertion of relat...

journal_title：Chromosoma

authors： Yamamoto Y,Gerbi SA

更新日期：2018-12-01 00:00:00

abstract：:In Drosophila gibberosa, differences between midgut and salivary gland chromosomes fall into two categories: tissue-specific band modulations which persist throughout the 90 h developmental period that we studied and tissue-specific puffs. Puffs that are common to both tissues tend to appear earlier in the midgut. Som...

journal_title：Chromosoma

authors： Roberts PA

更新日期：1988-11-01 00:00:00