Chromosome delineation induced by a combined potassium permanganate-sodium bisulfite treatment.

abstract：:The chromatin protein Polycomb (PC) is necessary for keeping homeotic genes repressed in a permanent and heritable manner. PC is part of a large multimeric complex (PcG proteins) involved in generating silenced chromatin domains at target genes, thus preventing their inappropriate expression. In order to assess the in...

journal_title：Chromosoma

authors： Dietzel S,Niemann H,Brückner B,Maurange C,Paro R

更新日期：1999-05-01 00:00:00

abstract：:The overall nuclease sensitivity and methylation of active and inactive X chromosomes of kangaroos were examined by in situ nick translation. Cultured fibroblasts of subspecies wallaroo-euro (Macropus robustus robustus; Macropus robustus erubescens) hybrids were used, enabling the paternally and maternally derived X c...

journal_title：Chromosoma

authors： Loebel DA,Johnston PG

更新日期：1993-01-01 00:00:00

abstract：:The mean DNA content of G2 macronuclei varies during the life cycle of the ciliate Tetrahymena thermophila. Early in the life cycle the mean is about 130 C; later it is about 94 C. In hybrids between strains A and B the decrease from 130 C to 94 C usually began after 60 fissions after conjugation. In B X B clones the ...

journal_title：Chromosoma

authors： Doerder FP,DeBault LE

更新日期：1978-10-20 00:00:00

abstract：:Simple repeated GATA and GACA sequences which were originally isolated from sex-specific snake satellite DNA have been found subsequently in all eukaryotes studied. The organization of these sequences within the mouse genome was investigated here by using synthetic oligonucleotide probes as a novel tool in comparison ...

journal_title：Chromosoma

authors： Schäfer R,Ali S,Epplen JT

更新日期：1986-01-01 00:00:00

abstract：:It is reported that chromatin can be prepared from highly purified polytene nuclei from the salivary glands of third instar larvae of Drosophila hydei; such chromatin differs from that of diploid nuclei mainly by deficiencies in certain nonhistone chromosomal proteins. It is suggested that these proteins are important...

journal_title：Chromosoma

authors： Elgin SC,Boyd JB

更新日期：1975-01-01 00:00:00

abstract：:At meiotic prophase the chromatin becomes arranged in loops on newly formed chromosome cores. The cores of homologous chromosomes become aligned in parallel and thus form the synaptonemal complex (SC), a structure found in the meiocytes of nearly all recombinationally competent, sexually reproducing organisms. We repo...

journal_title：Chromosoma

authors： Moens PB,Earnshaw WC

更新日期：1989-11-01 00:00:00

abstract：:The location of DNA fragments representing mobile dispersed genes (MDG) in salivary gland and midgut polytene chromosomes was compared by means of in situ hybridization. In the Drosophila stock under study the average number of hybridization sites in the polytene chromosomes of one nucleus was 20 for MDG-1 and 10 for ...

journal_title：Chromosoma

authors： Ananiev EV,Ilyin YV

更新日期：1981-01-01 00:00:00

abstract：:Isolated nuclei of the unicellular alga Olisthodiscus luteus, the uninucleate dinoflagellate Crypthecodinium cohnii and the binucleate dinoflagellate Peridinium balticum were lysed and deposited on grids by the microcentrifugation technique. The ultrastructure of the released chromatin fibers was compared to that of m...

journal_title：Chromosoma

authors： Rizzo PJ,Burghardt RC

更新日期：1980-01-01 00:00:00

abstract：:The organization of interphase chromatin spans many topics, ranging in scale from the molecular level to the whole nucleus, and its study requires a concomitant range of experimental approaches. In this review, we examine these approaches, the results they have generated, and the interfaces between them. The greatest ...

journal_title：Chromosoma

authors： Horowitz-Scherer RA,Woodcock CL

更新日期：2006-02-01 00:00:00

abstract：:Metaphase chromosomes from the Chinese hamster cell line M3-1 were separated by means of a flow sorter. Two chromosome fractions were used for this study: A, which consisted of 95% pure chromosome no. 1, and B, which was 90% pure chromosome no. 2. The DNA of 10(6) chromosomes of each type was purified, and a 125I-cRNA...

journal_title：Chromosoma

authors： Sawin VL,Rowley JD,Carrano AV

更新日期：1979-01-31 00:00:00

abstract：:By taking advantage of the fact that fluorescent light (FL) induces strand breaks only in bromodeoxyuridine(BrdUy-substituted DNA, and that those breaks eventually lead to the formation of sister chromatid exchanges (SCEs), the response of SCEs to FL was studied carefully in Chinese hamster chromosomes in which, out o...

journal_title：Chromosoma

authors： Kato H

更新日期：1977-02-03 00:00:00

abstract：:The ribosome is responsible for the final step of decoding genetic information into proteins. Therefore, correct assembly of ribosomes is a fundamental task for all living cells. In eukaryotes, the construction of the ribosome which begins in the nucleolus requires coordinated efforts of >350 specialized factors that ...

journal_title：Chromosoma

authors： Gerhardy S,Menet AM,Peña C,Petkowski JJ,Panse VG

更新日期：2014-08-01 00:00:00

abstract：:We provide evidence that centromere-specific 155 bp DNA repeats terminate one pair of telomeres at the telocentric, left end of the short fourth chromosome in Chironomus pallidivittatus. Earlier evidence indicated that all other telomeres are terminated by 340 bp telomere-specific repeats. DNA that borders the 155 bp ...

journal_title：Chromosoma

authors： Rosén M,Castillejo-López C,Edström JE

更新日期：2002-02-01 00:00:00

abstract：:Chromosome paints of the rat kangaroo ( Aepyprymnus rufuscens, 2 n=32) were used to define chromosome regions in the long nosed potoroo ( Potorous tridactylus, 2 n=12 female, 13 male) karyotype and localize these regions in three-dimensionally preserved nuclei of the potoroo to test the hypothesis that marsupial chrom...

journal_title：Chromosoma

authors： Rens W,O'Brien PC,Graves JA,Ferguson-Smith MA

更新日期：2003-08-01 00:00:00

abstract：:We determined the structure of the Schizosaccharomyces pombe centromere cen3 using direct genomic mapping and cosmid walking. The repetitive region of cen3 is approximately 110 kb, much longer than that of the previously determined cen1 and cen2 regions. The approximately 30 kb long left and approximately 60 kb right ...

journal_title：Chromosoma

authors： Murakami S,Matsumoto T,Niwa O,Yanagida M

更新日期：1991-12-01 00:00:00

abstract：:In the domestic pig (2n = 38) two types of constitutive heterochromatin can be differentiated by fluorescence counterstaining techniques. All 24 biarmed autosomes and the X chromosome have chromomycin A3-positive centromeric C-bands, whereas all 12 acrocentric chromosomes exhibit DA-DAPI-positive centromeric heterochr...

journal_title：Chromosoma

authors： Schwarzacher T,Mayr B,Schweizer D

更新日期：1984-01-01 00:00:00

abstract：:Experiments were designed to test whether nonrandom segregation of sister chromatids at mitosis has a role in the production of cell diversity during embryogenesis, Segregation was examined in vivo in retinal cells from embryonic chicks. Chromatids were labelled with bromouracil and stained by the fluorescence plus Gi...

journal_title：Chromosoma

authors： Morris VB

更新日期：1977-03-16 00:00:00

abstract：:A simple method has been described for the visualization of chromosomes cores with light microscopy in conventional chromosome preparations. The technique is relatively simple, highly reproducible and can be used effectively on fresh and aged slides. The following observations have been made: (1) a core existed in mit...

journal_title：Chromosoma

authors： Satya-Prakash KL,Hsu TC,Pathak S

更新日期：1980-01-01 00:00:00

abstract：:The method of ultrathin sections of unsquashed salivary gland polytene chromosomes of Ch. thummi was applied to their ultrastrual mapping. There was a good agreement between electron micrographs and Hägele's light microscopic map (1970) with respect to the pattern and number of bands. 94% of bands were identified in l...

journal_title：Chromosoma

authors： Kiknadze II,Perov NA,Chentsov YS

更新日期：1976-03-31 00:00:00

abstract：:To structurally dissect mitotic chromosomes, we aim to position along the folded chromatin fiber proteins involved in long-range order, such as topoisomerase IIalpha (topoIIalpha) and condensin. Immuno-electron microscopy (EM) of thin-sectioned chromosomes is the method of choice toward this goal. A much-improved immu...

journal_title：Chromosoma

authors： Maeshima K,Eltsov M,Laemmli UK

更新日期：2005-11-01 00:00:00

abstract：:The hsp70, hsp83, hsromega, and the small heat shock protein genes were mapped on the polytene chromosomes of six species, representative of the geographical distribution of the Drosophila montium subgroup of the melanogaster species group. In addition, based on hybridization conditions, the putative locus of the hsp6...

journal_title：Chromosoma

authors： Drosopoulou E,Konstantopoulou I,Scouras ZG

更新日期：1996-08-01 00:00:00

abstract：:The role of helix 6, which forms the major portion of the most 5'-located expansion segment of Saccharomyces cerevisiae 18S rRNA, was studied by in vivo mutational analysis. Mutations that increased the size of the helical part and/or the loop, even to a relatively small extent, abolished 18S rRNA formation almost com...

journal_title：Chromosoma

authors： van Nues RW,Venema J,Planta RJ,Raué HA

更新日期：1997-06-01 00:00:00

abstract：:Drosophila melanogaster was transformed with an 18 kb fragment of the C3 DNA puff of Rhynchosciara americana, including the C3-22 gene and the origins of replication that direct amplification. Different tissues and developmental stages of five independent transgenic lines were analyzed by quantitative Southern blot hy...

journal_title：Chromosoma

authors： Soares MA,Monesi N,Basso LR Jr,Stocker AJ,Paçó-Larson ML,Lara FJ

更新日期：2003-10-01 00:00:00

abstract：:The order Monotremata, comprising the platypus and two species of echidna (Australian and Nuigini) is the only extant representative of the mammalian subclass Prototheria, which diverged from subclass Theria (marsupials and placental mammals) 150-200 million years ago. The 2n = 63 male, 64 female karyotype (newly desc...

journal_title：Chromosoma

authors： Wrigley JM,Graves JA

更新日期：1988-01-01 00:00:00

abstract：:The frequency and distribution of chiasmata and the nature of terminal "associations" was re-examined in Syrian hamster spermatocytes using the 5-bromo-2'-deoxyuridine (BrdU) antibody technique (BAT) for differential chromatid labelling. Differential chromatid substitution was achieved by BrdU incorporation at the pen...

journal_title：Chromosoma

authors： Latos-Bielenska A,Vogel W

更新日期：1990-08-01 00:00:00

abstract：:The antigens in HeLa and Trichosia pubescens cells, recognized by sera from patients with rheumatic diseases containing anti-Ku antibodies, were compared by means of immunoprecipitation of labeled cell extracts. The autoantibodies present in the tested sera precipitate at least two polypeptides of approximately Mr = 7...

journal_title：Chromosoma

authors： Amabis JM,Amabis DC,Kaburaki J,Stollar BD

更新日期：1990-04-01 00:00:00

abstract：:The binding of methylene blue to DNA and chromatin treated in various ways was examined by equilibrium dialysis. The maximum r value (moles of bound dye/mole of nucleotide) was 1.0 for DNA, 0.6 for unfixed chromatin, and 0.83 for chromatin fixed in methanol-acetic acid. When fixed chromatin was treated with saline-cit...

journal_title：Chromosoma

authors： Comings DE,Avelino E

更新日期：1975-08-11 00:00:00

abstract：:During DNA synthesis there is a distinct stage immediately after the joining of large DNA replication intermediates (post-elongation stage). The conversion of this DNA to mature DNA was analysed in cells treated with aphidicolin to stop the movement of the replication fork. In such cells mature DNA is formed. In contr...

journal_title：Chromosoma

authors： Lönn U,Lönn S

更新日期：1987-01-01 00:00:00

abstract：:Chromosome behaviour during meiosis in male Syrian hamsters heterozygous for one of three translocations was analysed as part of a study of the transmission of these structural changes. Synapsis was studied using preparations of synaptonemal complexes, and chiasmate associations and the results of anaphase I segregati...

journal_title：Chromosoma

authors： Cawood AH,Breckon G

更新日期：1989-10-01 00:00:00

abstract：:Membrane-free folded chromosomes were purified from log-phase cultures of Escherichia coli and prepared for electron microscopy by aqueous (Kleinschmidt and Zahn) spreading. The appearance of the chromosomes depended on the salt concentrations in spreading. At certain salt concentration, the chromosomes resembled rose...

journal_title：Chromosoma

authors： Kavenoff R,Bowen BC

更新日期：1976-12-16 00:00:00