Protein constitution of the chromosome axis.

abstract：:The cotyledon parenchyma cells of common bean (Phaseolus vulgaris L.) produce large quantities of storage proteins during embryo maturation; throughout this period, these cells also accumulate nuclear DNA (nDNA). To investigate the basis of this nDNA accumulation, we have measured storage protein mRNA pools, nDNA mass...

journal_title：Chromosoma

authors： Johnson KA,Sussex IM

更新日期：1990-07-01 00:00:00

abstract：:We have used a new approach involving in situ hybridisation and electron microscopy to establish ultrastructural homologies between polytene chromosome regions of Drosophila melanogaster and Drosophila subobscura. Twelve probes were chosen to cover all the chromosomal elements: the myospheroid gene, the collagen type ...

journal_title：Chromosoma

authors： Cuenca JB,Galindo MI,Saura AO,Sorsa V,de Frutos R

更新日期：1998-05-01 00:00:00

abstract：:The divergence of lineages leading to extant squamate reptiles (lizards, snakes, and amphisbaenians) and birds occurred about 275 million years ago. Birds, unlike squamates, have karyotypes that are typified by the presence of a number of very small chromosomes. Hence, a number of chromosome rearrangements might be ex...

journal_title：Chromosoma

authors： Pokorná M,Giovannotti M,Kratochvíl L,Kasai F,Trifonov VA,O'Brien PC,Caputo V,Olmo E,Ferguson-Smith MA,Rens W

更新日期：2011-10-01 00:00:00

abstract：:The molecular structure of chromatin during dogfish spermiogenesis was examined by electron microscopy after the dispersion of nuclei at low ionic strength. In early and late stages of differentiation (round and elongating spermatids), chromatin is globular, although basic nuclear proteins are different from those pre...

journal_title：Chromosoma

authors： Gusse M,Chevaillier P

更新日期：1980-01-01 00:00:00

abstract：:Cyclins, as regulatory partners of cyclin-dependent kinases (CDKs), control the switch-like cell cycle transitions that orchestrate orderly duplication and segregation of genomes. Compared to mitosis, relatively little is known about how cyclin-CDK complexes control meiosis, the specialized cell division that generate...

journal_title：Chromosoma

authors： Karasu ME,Keeney S

更新日期：2019-09-01 00:00:00

abstract：:Studies of gene expression in aneuploids have often made the assumption that measurements of RNA abundance from the varied chromosome will establish whether there is a dosage effect or compensation. Typical procedures of RNA isolation and use of equal amounts of RNA for quantitative estimates will not measure the tota...

journal_title：Chromosoma

authors： Birchler JA

更新日期：2014-10-01 00:00:00

abstract：:We show that methylated lysine 9 of histone H3 (Me9H3) is a marker of heterochromatin in divergent animal species. It localises to both constitutive and facultative heterochromatin and replicates late in S-phase of the cell cycle. Significantly, Me9H3 is enriched in the inactive mammalian X chromosome (Xi) in female c...

journal_title：Chromosoma

authors： Cowell IG,Aucott R,Mahadevaiah SK,Burgoyne PS,Huskisson N,Bongiorni S,Prantera G,Fanti L,Pimpinelli S,Wu R,Gilbert DM,Shi W,Fundele R,Morrison H,Jeppesen P,Singh PB

更新日期：2002-03-01 00:00:00

abstract：:The ribosome is responsible for the final step of decoding genetic information into proteins. Therefore, correct assembly of ribosomes is a fundamental task for all living cells. In eukaryotes, the construction of the ribosome which begins in the nucleolus requires coordinated efforts of >350 specialized factors that ...

journal_title：Chromosoma

authors： Gerhardy S,Menet AM,Peña C,Petkowski JJ,Panse VG

更新日期：2014-08-01 00:00:00

abstract：:The distribution of constitutive heterochromatin has been investigated in four chromosomal races of the grasshopper Caledia captiva (2n=23 male/24 female) by the C-banding technique. Each of the four races was found to have a distinctive banding pattern which is associated with the inter-racial differences in chromoso...

journal_title：Chromosoma

authors： Shaw DD,Webb GC,Wilkinson P

更新日期：1976-06-30 00:00:00

abstract：:It is reported that chromatin can be prepared from highly purified polytene nuclei from the salivary glands of third instar larvae of Drosophila hydei; such chromatin differs from that of diploid nuclei mainly by deficiencies in certain nonhistone chromosomal proteins. It is suggested that these proteins are important...

journal_title：Chromosoma

authors： Elgin SC,Boyd JB

更新日期：1975-01-01 00:00:00

abstract：:Retroviruses have the ability to integrate into the genome of their host, in many cases with little apparent sequence or site specificity. However, relatively few studies have addressed more general features of chromosomal integration. In this study we directly visualized the chromosomal organization of three represen...

journal_title：Chromosoma

authors： Taruscio D,Manuelidis L

更新日期：1991-12-01 00:00:00

abstract：:Polo-like kinase 1 (Plk1) and Aurora A play key roles in centrosome maturation, spindle assembly, and chromosome segregation during cell division. Here we show that the functions of these kinases during early mitosis are coordinated through Bora, a partner of Aurora A first identified in Drosophila. Depletion of human...

journal_title：Chromosoma

authors： Chan EH,Santamaria A,Silljé HH,Nigg EA

更新日期：2008-10-01 00:00:00

abstract：:DNA reassociation kinetics were studied, by means of the hydroxyapatite chromatography method, for four species of Amphibians with different nuclear DNA content: Xenopus laevis (3 pg DNA per haploid genome) and Bufo bufo (7 pg) of the Anura subclass and Triturus cristatus (23 pg) and Necturus maculosus (52 pg) of the ...

journal_title：Chromosoma

authors： Baldari CT,Amaldi F

更新日期：1976-12-06 00:00:00

abstract：:From a "shotgun" collection of DNA fragments, isolated from Drosophila melanogaster, we selected sequences that function as autonomously replicating sequences (ARS) in the yeast Saccharomyces cerevisiae. To investigate the replicative potential of such sequences in Drosophila, five of these ARS elements and also the A...

journal_title：Chromosoma

authors： Roth GE

更新日期：1991-05-01 00:00:00

abstract：:Human neutrophil nuclei typically consist of three of four large heterochromatic lobes joined by thin, DNA-containing filaments. In addition, some lobes exhibit appendages of various sizes and shapes. Classical genetic and cytological studies suggest that some appendages contain specific chromosomes. The studies repor...

journal_title：Chromosoma

authors： Aquiles Sanchez J,Karni RJ,Wangh LJ

更新日期：1997-08-01 00:00:00

abstract：:The nuclear topography of pericentromeric DNA of chromosome 11 was analyzed in G0 (nonstimulated) and G1 [phytohemagglutinin (PHA) stimulated] human lymphocytes by confocal microscopy. In addition to the nuclear center, the centrosome was used as a second point of reference in the three-dimensional (3D) analysis. Peri...

journal_title：Chromosoma

authors： Hulspas R,Houtsmuller AB,Krijtenburg PJ,Bauman JG,Nanninga N

更新日期：1994-07-01 00:00:00

abstract：:Recent data suggest that insulators organize chromatin architecture in the nucleus. The best characterized Drosophila insulator, found in the gypsy retrotransposon, contains 12 binding sites for the Su(Hw) protein. Enhancer blocking, along with Su(Hw), requires BTB/POZ domain proteins, Mod(mdg4)-67.2 and CP190. Inacti...

journal_title：Chromosoma

authors： Melnikova L,Shapovalov I,Kostyuchenko M,Georgiev P,Golovnin A

更新日期：2017-03-01 00:00:00

abstract：:A complete human metaphase chromosome has been reconstructed from a series of electron microscopical projections obtained by tilting the specimen stage at 3 degree intervals from -60 to +60 degrees. The reconstructed structure is about 3.0 microns long, 1.6 micron wide, and 0.8 micron thick. The mass distribution was ...

journal_title：Chromosoma

authors： Harauz G,Borland L,Bahr GF,Zeitler E,van Heel M

更新日期：1987-01-01 00:00:00

abstract：:We sought for cloned sequences of middle repetitive (MR) complexity that mark obligatory heterochromatic regions. Total genome probes were employed in a differential screening procedure to recover X-specific, Y-specific and autosomal specific heterochromatic sequences. X-and Y-linked sequences were recovered in the sa...

journal_title：Chromosoma

authors： Lifschytz E,Hareven D

更新日期：1982-01-01 00:00:00

abstract：:The mean DNA content of G2 macronuclei varies during the life cycle of the ciliate Tetrahymena thermophila. Early in the life cycle the mean is about 130 C; later it is about 94 C. In hybrids between strains A and B the decrease from 130 C to 94 C usually began after 60 fissions after conjugation. In B X B clones the ...

journal_title：Chromosoma

authors： Doerder FP,DeBault LE

更新日期：1978-10-20 00:00:00

abstract：:The MRE11/RAD50/NBS1 complex (Mre11 complex) is a central player in most aspects of the cellular response to DNA double-strand breaks, including homologous recombination, non-homologous end joining, telomere maintenance and DNA damage checkpoint activation. Several of these findings are explained by the unusual enzyma...

journal_title：Chromosoma

authors： Assenmacher N,Hopfner KP

更新日期：2004-10-01 00:00:00

abstract：:Chromosome replication in the last premeiotic S-phase of male mammals has been previously studied by [3H]thymidine autoradiography and by a 5-bromodeoxyuridine (BrdU)/Giemsa technique. We used a recently developed BrdU-antibody technique (BAT) in this study. The following conclusions were drawn: (1) The replication pa...

journal_title：Chromosoma

authors： Latos-Bielenska A,Vogel W

更新日期：1992-03-01 00:00:00

abstract：:Nucleolar matrix structures were obtained under different extraction conditions from highly purified isolated nucleoli. Their ultrastructural appearance, protein composition and capacity to bind rDNA preferentially were studied in a model binding system. A region spanning approximately 25 kb in the rat ribosomal gene ...

journal_title：Chromosoma

authors： Stephanova E,Stancheva R,Avramova Z

更新日期：1993-03-01 00:00:00

abstract：:Knowledge about the composition and structure of centromeres is critical for understanding how centromeres perform their functional roles. Here, we report the sequences of one centromere-associated bacterial artificial chromosome clone from a Coix lacryma-jobi library. Two Ty3/gypsy-class retrotransposons, centromeric...

journal_title：Chromosoma

authors： Han Y,Wang G,Liu Z,Liu J,Yue W,Song R,Zhang X,Jin W

更新日期：2010-02-01 00:00:00

abstract：:Spermatogenic cells of the mouse have been separated by gravity sedimentation using a modification of a previously published method. Details are given for the collection of purified samples of specific meiotic stages and for the collection of labelled cell fractions following injection of 3H-thymidine. Suppression of ...

journal_title：Chromosoma

authors： Chandley AC,Hotta Y,Stern H

更新日期：1977-07-08 00:00:00

abstract：:Mammalian heterochromatin proteins 1 (HP1alpha, HP1beta, and HP1gamma) are nonhistone proteins that interact in vitro with a set of proteins that play a role in chromatin silencing, transcription, and chromatin remodeling. Using antibodies specific for each HP1 isoform, we showed that they segregate in distinct nuclea...

journal_title：Chromosoma

authors： Minc E,Allory Y,Worman HJ,Courvalin JC,Buendia B

更新日期：1999-08-01 00:00:00

abstract：:Changes in the nuclear positioning of specific genes, depending on their expression status, have been observed in a large diversity of physiological processes. However, gene position is poorly documented for immune cells which have been subjected to activation following bacterial infection. Using a pig model, we focus...

journal_title：Chromosoma

authors： Solinhac R,Mompart F,Martin P,Robelin D,Pinton P,Iannuccelli E,Lahbib-Mansais Y,Oswald IP,Yerle-Bouissou M

更新日期：2011-10-01 00:00:00

abstract：:Centromere protein B (CENP-B) is a centromeric DNA-binding protein that binds to alpha-satellite DNA at the 17 bp CENP-B box sequence. The binding of CENP-B, along with other proteins, to alpha-satellite DNA sequences at the centromere, is thought to package the DNA into heterochromatin subjacent to the kinetochore of...

journal_title：Chromosoma

authors： Kapoor M,Montes de Oca Luna R,Liu G,Lozano G,Cummings C,Mancini M,Ouspenski I,Brinkley BR,May GS

更新日期：1998-12-01 00:00:00

abstract：:The irradiation of metaphase spreads of human cells with ultraviolet (UV) light blocked the chromosome banding induced by Alu I, Mbo I, Dde I, Hinf I, Hae III, and Rsa I restriction endonucleases. At 13 J/m2 there was moderate inhibition of the nuclease action, which was detected as an increase in the stain intensity ...

journal_title：Chromosoma

authors： Bianchi NO,Bianchi MS,Cleaver JE

更新日期：1984-01-01 00:00:00

abstract：:The X and Y chromosomes of the musk shrew are the two largest in the complement and they regularly form a single chiasma during meiosis. This chiasma is located in the short arms of the X and Y, both of which show partial C-banding at meiosis. The in vitro incorporation of 5-bromodeoxyuridine/tritiated thymidine durin...

journal_title：Chromosoma

authors： Raman R,Nanda I

更新日期：1986-01-01 00:00:00