Abstract:
:Ring chromosomes are of basic interest to the geneticist and cell biologist who study their behavior in meiotic and mitotic divisions. In addition, the mitotic instability associated with some ring-X chromosomes has proven useful in Drosophila as a means to produce gynandromorphs for developmental studies. We describe a method to construct ring-X chromosomes in Drosophila via I-CreI-mediated exchange in rDNA, and then rapidly diagnose the recovery of ring chromosomes via FLP-mediated sister chromatid exchange within the ring. The method we describe provides a ready means to tailor the genetic content of ring-X chromosomes, making it suited to produce ring-X chromosomes for a variety of experimental purposes.
journal_name
Chromosomajournal_title
Chromosomaauthors
Golic MM,Golic KGdoi
10.1007/s00412-010-0297-2subject
Has Abstractpub_date
2011-04-01 00:00:00pages
159-64issue
2eissn
0009-5915issn
1432-0886journal_volume
120pub_type
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