A simple and rapid method for constructing ring-X chromosomes in Drosophila melanogaster.

Abstract:

:Ring chromosomes are of basic interest to the geneticist and cell biologist who study their behavior in meiotic and mitotic divisions. In addition, the mitotic instability associated with some ring-X chromosomes has proven useful in Drosophila as a means to produce gynandromorphs for developmental studies. We describe a method to construct ring-X chromosomes in Drosophila via I-CreI-mediated exchange in rDNA, and then rapidly diagnose the recovery of ring chromosomes via FLP-mediated sister chromatid exchange within the ring. The method we describe provides a ready means to tailor the genetic content of ring-X chromosomes, making it suited to produce ring-X chromosomes for a variety of experimental purposes.

journal_name

Chromosoma

journal_title

Chromosoma

authors

Golic MM,Golic KG

doi

10.1007/s00412-010-0297-2

subject

Has Abstract

pub_date

2011-04-01 00:00:00

pages

159-64

issue

2

eissn

0009-5915

issn

1432-0886

journal_volume

120

pub_type

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