High resolution mapping of in situ hybridized biotinylated DNA to surface-spread Drosophila polytene chromosomes.

abstract：:We present a general model for the evolution of chromosome-specific satellite DNA subfamilies. Sus scrofa domestica has a bimodal karyotype with two autosomal subsets of 12 meta-/submetacentric (Mc) and 6 acrocentric (Ac) chromosome types (Mc and Ac "subgenomes"). We show that the centromeric heterochromatin is charac...

journal_title：Chromosoma

authors： Jantsch M,Hamilton B,Mayr B,Schweizer D

更新日期：1990-09-01 00:00:00

abstract：:Osteosarcoma (OS) is characterized by chromosomal instability and high copy number gene amplification. The breakage-fusion-bridge (BFB) cycle is a well-established mechanism of genome instability in tumors and in vitro models used to study the origins of complex chromosomal rearrangements and cancer genome amplificati...

journal_title：Chromosoma

authors： Selvarajah S,Yoshimoto M,Park PC,Maire G,Paderova J,Bayani J,Lim G,Al-Romaih K,Squire JA,Zielenska M

更新日期：2006-12-01 00:00:00

abstract：:Membrane-free folded chromosomes were purified from log-phase cultures of Escherichia coli and prepared for electron microscopy by aqueous (Kleinschmidt and Zahn) spreading. The appearance of the chromosomes depended on the salt concentrations in spreading. At certain salt concentration, the chromosomes resembled rose...

journal_title：Chromosoma

authors： Kavenoff R,Bowen BC

更新日期：1976-12-16 00:00:00

abstract：:By using the optical properties of birefringence of DNA, the arrangement of these molecules has been studied in Dinoflagellate chromosomes and Dipteran polytene chromosomes. These latter are used, here, as a reference material. These observations have been made under a polarizing microscope on intact and stretched chr...

journal_title：Chromosoma

authors： Livolant F

更新日期：1978-08-21 00:00:00

abstract：:The spindle checkpoint restrains anaphase onset and mitotic exit until all chromosomes are stably attached to the mitotic spindle via their kinetochores. The Tao1 protein kinase was recently reported as a novel spindle checkpoint component. When an siRNA was used to repress Tao1, the essential spindle checkpoint compo...

journal_title：Chromosoma

authors： Westhorpe FG,Diez MA,Gurden MD,Tighe A,Taylor SS

更新日期：2010-08-01 00:00:00

abstract：:Facioscapulohumeral muscular dystrophy (FSHD) is caused by the shortening of a copy-number polymorphic array of 3.3 kb repeats (D4Z4) at one allelic 4q35.2 region. How this contraction of a subtelomeric tandem array causes FSHD is unknown but indirect evidence suggests that a short array has a cis effect on a distant ...

journal_title：Chromosoma

authors： Yang F,Shao C,Vedanarayanan V,Ehrlich M

更新日期：2004-05-01 00:00:00

abstract：:Chromosomal replication was studied by means of the BrdU-Höchst-Giemsa-technique in three minute T-cell leukemias with special regard to chromosome 15. In all the three leukemic cell lines the normally early replicating band 15 E was undetectable whereas late replication in this region is completely unaltered. Further...

journal_title：Chromosoma

authors： Somssich IE,Spira J,Hameister H,Klein G

更新日期：1982-01-01 00:00:00

abstract：:The study of RNA pol II-mediated transcription regulation has been dominated by molecular biological approaches. Although these methods continue to provide important insights, other approaches are required to insure against an oversimplified view of gene expression. Improvements in EM methods and the development of th...

journal_title：Chromosoma

authors： Hendzel MJ,Bazett-Jones DP

更新日期：1995-02-01 00:00:00

abstract：:The nuclear topography of pericentromeric DNA of chromosome 11 was analyzed in G0 (nonstimulated) and G1 [phytohemagglutinin (PHA) stimulated] human lymphocytes by confocal microscopy. In addition to the nuclear center, the centrosome was used as a second point of reference in the three-dimensional (3D) analysis. Peri...

journal_title：Chromosoma

authors： Hulspas R,Houtsmuller AB,Krijtenburg PJ,Bauman JG,Nanninga N

更新日期：1994-07-01 00:00:00

abstract：:By taking advantage of the fact that fluorescent light (FL) induces strand breaks only in bromodeoxyuridine(BrdUy-substituted DNA, and that those breaks eventually lead to the formation of sister chromatid exchanges (SCEs), the response of SCEs to FL was studied carefully in Chinese hamster chromosomes in which, out o...

journal_title：Chromosoma

authors： Kato H

更新日期：1977-02-03 00:00:00

abstract：:cDNA, copied from nuclear RNA isolated from heat shocked Drosophila hydei cells, has been cloned. From this collection of clones a clone, N09-15, with a 450 bp insert has been isolated that hybridizes in situ to the heat shock locus-2-48B of Drosophila hydei. The N09-15 sequence is present in two different genomic arr...

journal_title：Chromosoma

authors： Peters FP,Grond CJ,Sondermeijer PJ,Lubsen NH

更新日期：1982-01-01 00:00:00

abstract：:After conjugation in the ciliated protozoan, Oxytricha, polytene chromosomes are formed during the development of a macronucleus from a micronucleus. Here we report a microscopic study of these chromosomes and an analysis of their DNA. The polytene chromosomes of Oxytricha bear a strong morphological resemblance to th...

journal_title：Chromosoma

authors： Spear BB,Lauth MR

更新日期：1976-01-27 00:00:00

abstract：:Using BrdU/Hoechst 33258/Giemsa methods for detecting replicating chromosome bands, a method is described by which the DNA synthesis phase may be sub-divided on the basis of distinctive patterns displayed by certain chromosomes.--Applied to asynchronous populations successively sampled through one cell cycle, cells in...

journal_title：Chromosoma

authors： Savage JR,Bhunya SP

更新日期：1980-01-01 00:00:00

abstract：:The location of DNA fragments representing mobile dispersed genes (MDG) in salivary gland and midgut polytene chromosomes was compared by means of in situ hybridization. In the Drosophila stock under study the average number of hybridization sites in the polytene chromosomes of one nucleus was 20 for MDG-1 and 10 for ...

journal_title：Chromosoma

authors： Ananiev EV,Ilyin YV

更新日期：1981-01-01 00:00:00

abstract：:Meiotic pairing and segregation were studied in three different heterozygous reciprocal translocation strains of the baker's yeast, Saccharomyces cerevisiae. Pachytene translocation quadrivalents were identified by a combination of immunofluorescence and fluorescence in situ hybridization and the karyotypes of meiotic...

journal_title：Chromosoma

authors： Loidl J,Jin QW,Jantsch M

更新日期：1998-09-01 00:00:00

abstract：:The sub-division of S-phase in Syrian hamsters, on the basis of Brd/U/Hoechst 33258/Giemsa banding, has allowed a quantitative comparison of the replication of individual chromosome bands within defined subphases of S. This analysis has shown that in hamsters, as has been reported in humans, there are distinct pattern...

journal_title：Chromosoma

authors： Cawood AH

更新日期：1981-01-01 00:00:00

abstract：:In mammals, X-inactivation process is achieved by the cis-spreading of long noncoding Xist RNA over one of the female X chromosomes. The Xist binding accumulates histones H3 methylation and H4 hypoacetylation required for X inactivation that leads to proper dosage compensation of the X-linked genes. Co-transcription o...

journal_title：Chromosoma

authors： Kota SK,Roy Chowdhury D,Rao LK,Padmalatha V,Singh L,Bhadra U

更新日期：2015-06-01 00:00:00

abstract：:A simple method has been described for the visualization of chromosomes cores with light microscopy in conventional chromosome preparations. The technique is relatively simple, highly reproducible and can be used effectively on fresh and aged slides. The following observations have been made: (1) a core existed in mit...

journal_title：Chromosoma

authors： Satya-Prakash KL,Hsu TC,Pathak S

更新日期：1980-01-01 00:00:00

abstract：:Chromosome segregation in primary spermatocytes of the crane fly Nephrotoma suturalis was studied after exposure to Colcemid at doses that did not completely inhibit spindle formation. Colcemid was added either to the medium in which larvae were cultured or to Tricine buffer in which isolated testes were incubated. Pa...

journal_title：Chromosoma

authors： LaFountain JR Jr

更新日期：1985-01-01 00:00:00

abstract：:We studied the karyotype of specimens of Callithrix emiliae (Callithricidae, Primates) from Rondonia, Brazil. Comparison with the karyotype of Callithrix jacchus showed that, even though these two species show many karyotypic similarities, they differ by a Robertsonian translocation, a paracentric inversion and large-...

journal_title：Chromosoma

authors： de Souza Barros RM,Nagamachi CY,Pieczarka JC

更新日期：1990-10-01 00:00:00

abstract：:Dioecious Silene latifolia evolved heteromorphic sex chromosomes within the last ten million years, making it a species of choice for studies of the early stages of sex chromosome evolution in plants. About a dozen genes have been isolated from its sex chromosomes and basic genetic and deletion maps exist for the X an...

journal_title：Chromosoma

authors： Howell EC,Armstrong SJ,Filatov DA

更新日期：2011-06-01 00:00:00

abstract：:We determined the structure of the Schizosaccharomyces pombe centromere cen3 using direct genomic mapping and cosmid walking. The repetitive region of cen3 is approximately 110 kb, much longer than that of the previously determined cen1 and cen2 regions. The approximately 30 kb long left and approximately 60 kb right ...

journal_title：Chromosoma

authors： Murakami S,Matsumoto T,Niwa O,Yanagida M

更新日期：1991-12-01 00:00:00

abstract：:The X and Y chromosomes of the musk shrew are the two largest in the complement and they regularly form a single chiasma during meiosis. This chiasma is located in the short arms of the X and Y, both of which show partial C-banding at meiosis. The in vitro incorporation of 5-bromodeoxyuridine/tritiated thymidine durin...

journal_title：Chromosoma

authors： Raman R,Nanda I

更新日期：1986-01-01 00:00:00

abstract：:The concept of the nuclear matrix, a karyoskeletal structure that serves as a support for the genome and its activities, has stimulated many studies of the association of nuclear components and functions with this structure. However, certain experimental findings are not consistent with the existence of the nuclear ma...

journal_title：Chromosoma

authors： Hancock R

更新日期：2000-07-01 00:00:00

abstract：:Simple repeated GATA and GACA sequences which were originally isolated from sex-specific snake satellite DNA have been found subsequently in all eukaryotes studied. The organization of these sequences within the mouse genome was investigated here by using synthetic oligonucleotide probes as a novel tool in comparison ...

journal_title：Chromosoma

authors： Schäfer R,Ali S,Epplen JT

更新日期：1986-01-01 00:00:00

abstract：:Using fluorescence in situ hybridization, conventional epifluorescence microscopy, and laser scanning confocal microscopy followed by three-dimensional reconstruction we describe a well-defined higher order packaging of the human genome in the sperm cell nucleus. This was determined by the spatial localization of cent...

journal_title：Chromosoma

authors： Zalensky AO,Allen MJ,Kobayashi A,Zalenskaya IA,Balhórn R,Bradbury EM

更新日期：1995-05-01 00:00:00

abstract：:We have used a new approach involving in situ hybridisation and electron microscopy to establish ultrastructural homologies between polytene chromosome regions of Drosophila melanogaster and Drosophila subobscura. Twelve probes were chosen to cover all the chromosomal elements: the myospheroid gene, the collagen type ...

journal_title：Chromosoma

authors： Cuenca JB,Galindo MI,Saura AO,Sorsa V,de Frutos R

更新日期：1998-05-01 00:00:00

abstract：:The 5S RNA gene content of polyploid cells of the ovary of Drosophila melanogaster has been compared in animals with two or three gene clusters. The amount of 5S RNA genes is exactly proportional to the number of gene clusters as determined by DNA-RNA filter hybridization. In contrast, the number of rDNA genes in endo...

journal_title：Chromosoma

authors： Renkawitz-Pohl R

更新日期：1979-01-31 00:00:00

abstract：:Mutations in the MCPH1 gene result in primary microcephaly in combination with a unique cellular phenotype of defective chromosome condensation. MCPH1 patient cells display premature chromosome condensation in G2 phase of the cell cycle and delayed decondensation in early G1 phase, observable as an increased proportio...

journal_title：Chromosoma

authors： Arroyo M,Trimborn M,Sánchez A,Hirano T,Neitzel H,Marchal JA

更新日期：2015-12-01 00:00:00

abstract：:To structurally dissect mitotic chromosomes, we aim to position along the folded chromatin fiber proteins involved in long-range order, such as topoisomerase IIalpha (topoIIalpha) and condensin. Immuno-electron microscopy (EM) of thin-sectioned chromosomes is the method of choice toward this goal. A much-improved immu...

journal_title：Chromosoma

authors： Maeshima K,Eltsov M,Laemmli UK

更新日期：2005-11-01 00:00:00