Meiotic chromosome behaviour reflects levels of sequence divergence in Sus scrofa domestica satellite DNA.

Abstract:

:We present a general model for the evolution of chromosome-specific satellite DNA subfamilies. Sus scrofa domestica has a bimodal karyotype with two autosomal subsets of 12 meta-/submetacentric (Mc) and 6 acrocentric (Ac) chromosome types (Mc and Ac "subgenomes"). We show that the centromeric heterochromatin is characterised by two distinct satellite DNA families designed Mc1 and Ac2. Mc1 is a diverse satellite family of the Mc subgenome of which certain members with a 100 bp repeat unit are found to occur at the pericentromeric regions of each Mc autosome, while others are chromosome-specific, e.g. clone Mc pAv1.5, a higher order repeat variant, which hybridises specifically to chromosome 1. Ac2 is a homogeneous satellite occurring at the subterminal pericentromeric regions of all Ac autosomes. DNA sequence analyses showed that all clones investigated are built up from a 14 bp repeat unit which is highly conserved. In situ hybridisation to meiotic pachytene nuclei revealed a distinct spatial arrangement of the Ac2 centromeric satellite.

journal_name

Chromosoma

journal_title

Chromosoma

authors

Jantsch M,Hamilton B,Mayr B,Schweizer D

doi

10.1007/BF01731720

subject

Has Abstract

pub_date

1990-09-01 00:00:00

pages

330-5

issue

5

eissn

0009-5915

issn

1432-0886

journal_volume

99

pub_type

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