Abstract:
:The distribution patterns of chromosomal proteins from Drosophila can be observed by immunofluorescent staining of the polytene chromosomes from larval salivary glands. We have purified a non-histone chromosomal protein of Mr = 69,000 molecular weight which has a high affinity for DNA with little sequence specificity. Immunofluorescent staining indicates that this protein is preferentially associated with the inactive portions of the genome, including the centric heterochromatin and the condensed bands within the euchromatic arms of the chromosomes. Observation of both the heat shock loci 87A and 87C and the developmentally regulated loci 74EF and 75B shows an inverse correlation between immunofluorescent staining for the Mr = 69,000 protein and for RNA polymerase. The presence of this protein appears to be correlated with the packaging of the chromatin in an inactive form.
journal_name
Chromosomajournal_title
Chromosomaauthors
Fleischmann G,Filipski R,Elgin SCdoi
10.1007/BF00285889subject
Has Abstractpub_date
1987-01-01 00:00:00pages
83-90issue
1eissn
0009-5915issn
1432-0886journal_volume
96pub_type
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