New evidence for tandem chromosome fusions in the karyotypic evolution of Asian muntjacs.

abstract：:Position-effect variegation (PEV) is the mosaic expression of a euchromatic gene brought into juxtaposition with heterochromatin. Fourteen different transformed Drosophila melanogaster lines with variegating P-element inserts were used to examine the DNA levels of these transgenes. Insert sites include pericentric, te...

journal_title：Chromosoma

authors： Wallrath LL,Guntur VP,Rosman LE,Elgin SC

更新日期：1996-04-01 00:00:00

abstract：:We sought for cloned sequences of middle repetitive (MR) complexity that mark obligatory heterochromatic regions. Total genome probes were employed in a differential screening procedure to recover X-specific, Y-specific and autosomal specific heterochromatic sequences. X-and Y-linked sequences were recovered in the sa...

journal_title：Chromosoma

authors： Lifschytz E,Hareven D

更新日期：1982-01-01 00:00:00

abstract：:In Xenopus laevis eight tRNA genes are located in a 3.18 kb tandemly repeated unit. There are 150 copies of the unit at a single locus near the long arm telomere of one of the acrocentric chromosomes in the 14-17 group. Two additional classes of tRNA gene-containing repeats have been isolated (defined by clones p3.1 a...

journal_title：Chromosoma

authors： Narayanswami S,Doering JL,Fokta FJ,Rosenthal DS,Nguyen TN,Hamkalo BA

更新日期：1995-10-01 00:00:00

abstract：:Experiments were designed to test whether nonrandom segregation of sister chromatids at mitosis has a role in the production of cell diversity during embryogenesis, Segregation was examined in vivo in retinal cells from embryonic chicks. Chromatids were labelled with bromouracil and stained by the fluorescence plus Gi...

journal_title：Chromosoma

authors： Morris VB

更新日期：1977-03-16 00:00:00

abstract：:BrdU-substituted Chinese hamster chromosomes were treated with a hog Na2HPO4 solution and stained with Giemsa to produce sister chromatid differential staining (SCD). The process of SCD was examined with the Nomarski differential interference microscope and the scanning electron microscope. After the Na2HPO4 treatment...

journal_title：Chromosoma

authors： Takayama S,Utsumi KR,Sasaki Y

更新日期：1981-01-01 00:00:00

abstract：:Targeted insertion of large pieces of DNA is an important goal of genetic engineering. However, this goal has been elusive since classical methods for homology-directed repair are inefficient and often not feasible in many systems. Recent advances are described here that enable site-specific genomic insertion of relat...

journal_title：Chromosoma

authors： Yamamoto Y,Gerbi SA

更新日期：2018-12-01 00:00:00

abstract：:Chromosome replication in the last premeiotic S-phase of male mammals has been previously studied by [3H]thymidine autoradiography and by a 5-bromodeoxyuridine (BrdU)/Giemsa technique. We used a recently developed BrdU-antibody technique (BAT) in this study. The following conclusions were drawn: (1) The replication pa...

journal_title：Chromosoma

authors： Latos-Bielenska A,Vogel W

更新日期：1992-03-01 00:00:00

abstract：:Chromosome fibers isolated from lymphocyte nuclei and prepared for electron microscopy by techniques designed to preserve their native structure have a distinctly knobby appearance, suggesting that DNA and protein are not distributed evenly along the fiber axis. Individual knobs (superbeads) are arranged in tandem and...

journal_title：Chromosoma

authors： Hozier J,Renz M,Nehls P

更新日期：1977-07-18 00:00:00

abstract：:Osteosarcoma (OS) is characterized by chromosomal instability and high copy number gene amplification. The breakage-fusion-bridge (BFB) cycle is a well-established mechanism of genome instability in tumors and in vitro models used to study the origins of complex chromosomal rearrangements and cancer genome amplificati...

journal_title：Chromosoma

authors： Selvarajah S,Yoshimoto M,Park PC,Maire G,Paderova J,Bayani J,Lim G,Al-Romaih K,Squire JA,Zielenska M

更新日期：2006-12-01 00:00:00

abstract：:The polypurine/polypyrimidine (PuPy) tracts present in the human genome are known to be scattered among and within chromosomes. In PuPy tract sequences, triplex formation occurs readily under physiological conditions, leaving single-stranded DNAs capable of hybridization with complementary single-stranded DNAs and RNA...

journal_title：Chromosoma

authors： Ohno M,Fukagawa T,Lee JS,Ikemura T

更新日期：2002-09-01 00:00:00

abstract：:The binding of methylene blue to DNA and chromatin treated in various ways was examined by equilibrium dialysis. The maximum r value (moles of bound dye/mole of nucleotide) was 1.0 for DNA, 0.6 for unfixed chromatin, and 0.83 for chromatin fixed in methanol-acetic acid. When fixed chromatin was treated with saline-cit...

journal_title：Chromosoma

authors： Comings DE,Avelino E

更新日期：1975-08-11 00:00:00

abstract：:A crucial step during eukaryotic initiation of DNA replication is the correct loading and activation of the replicative DNA helicase, which ensures that each replication origin fires only once. Unregulated DNA helicase loading and activation, as it occurs in cancer, can cause severe DNA damage and genomic instability....

journal_title：Chromosoma

authors： Tognetti S,Riera A,Speck C

更新日期：2015-03-01 00:00:00

abstract：:Meiotic pairing has now been shown to require heterochromatic homology and to be sensitive to repeat number in both male and female Drosophila. The increased pairing ability of repetitive sequences could be one reason that most eukaryotes allow the accumulation of tandem repeated elements. This may well be a reflectio...

journal_title：Chromosoma

authors： Irick H

更新日期：1994-03-01 00:00:00

abstract：:The ribosome is responsible for the final step of decoding genetic information into proteins. Therefore, correct assembly of ribosomes is a fundamental task for all living cells. In eukaryotes, the construction of the ribosome which begins in the nucleolus requires coordinated efforts of >350 specialized factors that ...

journal_title：Chromosoma

authors： Gerhardy S,Menet AM,Peña C,Petkowski JJ,Panse VG

更新日期：2014-08-01 00:00:00

abstract：:The zebra finch (Taeniopygia guttata) has a large Z chromosome and highly condensed W chromosome. We used the random amplified polymorphic DNA (RAPD) polymerase chain reaction (PCR) technique to isolate female-specific sequences ZBM1 and ZBM2. Southern blot hybridization to male and female zebra finch genomic DNA sugg...

journal_title：Chromosoma

authors： Itoh Y,Kampf K,Arnold AP

更新日期：2008-04-01 00:00:00

abstract：:Microspore culture stimulates immature pollen grains to develop into plants via tissue culture and is used routinely in many crop species to produce "doubled haploids": homozygous, true-breeding lines. However, microspore culture is also often used on material that does not have stable meiosis, such as interspecific h...

journal_title：Chromosoma

authors： Mwathi MW,Schiessl SV,Batley J,Mason AS

更新日期：2019-12-01 00:00:00

abstract：:Ribosomal DNA nontranscribed spacers in Drosophila virilis DNA have been examined in some detail by restriction site analysis of cloned segments of rDNA, nucleic acid hybridizations involving unfractionated rDNA, and base composition estimates. The overall G + C content of the spacer is 27--28%; this compares with 39%...

journal_title：Chromosoma

authors： Rae PM,Barnett T,Murtif VL

更新日期：1981-01-01 00:00:00

abstract：:The order Monotremata, comprising the platypus and two species of echidna (Australian and Nuigini) is the only extant representative of the mammalian subclass Prototheria, which diverged from subclass Theria (marsupials and placental mammals) 150-200 million years ago. The 2n = 63 male, 64 female karyotype (newly desc...

journal_title：Chromosoma

authors： Wrigley JM,Graves JA

更新日期：1988-01-01 00:00:00

abstract：:Cytogenetic analysis indicates that Balbiani Ring 2 (BR 2) in the two sibling species Chironomus tentans and Chironomus pallidivittatus arises from identifically banded segments in the salivary gland polytene chromosomes, although chromosomal rearrangements have occurred. In situ hybridization of BR 2 RNA to the polyt...

journal_title：Chromosoma

authors： Lambert B,Beermann W

更新日期：1975-01-01 00:00:00

abstract：:An inverted duplication of chromosome 15 [inv dup(15)] is the most common supernumerary marker chromosome, comprising approximately 50% of all chromosomes in this class. Structurally, the inv dup(15) is a mirror image with the central axis defining a distal break within either the heterochromatic alpha-satellite array...

journal_title：Chromosoma

authors： Wandstrat AE,Schwartz S

更新日期：2000-11-01 00:00:00

abstract：:The genomic DNA organisation patterns of four sauropsidian species, namely Python reticularis, Caiman crocodilus, Terrapene carolina triungius and Columba livia domestica were investigated by reassociation of short and long DNA fragments, by hyperchromicity measurements of reannealed fragments and by length estimation...

journal_title：Chromosoma

authors： Epplen JT,Diedrich U,Wagenmann M,Schmidtke J,Engel W

更新日期：1979-11-01 00:00:00

abstract：:Chromosome segregation in primary spermatocytes of the crane fly Nephrotoma suturalis was studied after exposure to Colcemid at doses that did not completely inhibit spindle formation. Colcemid was added either to the medium in which larvae were cultured or to Tricine buffer in which isolated testes were incubated. Pa...

journal_title：Chromosoma

authors： LaFountain JR Jr

更新日期：1985-01-01 00:00:00

abstract：:Retroviruses have the ability to integrate into the genome of their host, in many cases with little apparent sequence or site specificity. However, relatively few studies have addressed more general features of chromosomal integration. In this study we directly visualized the chromosomal organization of three represen...

journal_title：Chromosoma

authors： Taruscio D,Manuelidis L

更新日期：1991-12-01 00:00:00

abstract：:In contrast to yeast, plant interphase nuclei often display incomplete alignment (cohesion) along sister chromatid arms. Sister chromatid cohesion mediated by the multi-subunit cohesin complex is essential for correct chromosome segregation during nuclear divisions and for DNA recombination repair. The cohesin complex...

journal_title：Chromosoma

authors： Schubert V,Weissleder A,Ali H,Fuchs J,Lermontova I,Meister A,Schubert I

更新日期：2009-10-01 00:00:00

abstract：:Centromere protein B (CENP-B) is a centromeric DNA-binding protein that binds to alpha-satellite DNA at the 17 bp CENP-B box sequence. The binding of CENP-B, along with other proteins, to alpha-satellite DNA sequences at the centromere, is thought to package the DNA into heterochromatin subjacent to the kinetochore of...

journal_title：Chromosoma

authors： Kapoor M,Montes de Oca Luna R,Liu G,Lozano G,Cummings C,Mancini M,Ouspenski I,Brinkley BR,May GS

更新日期：1998-12-01 00:00:00

abstract：:Among his many contributions to the field of chromosome structure and dynamics, J. Herbert Taylor showed that eukaryotic cells have many more potential replication origins than they use, which they can recruit when replication forks are slowed to complete S-phase in a timely fashion. Thirty years later, his findings r...

journal_title：Chromosoma

authors： Gilbert DM

更新日期：2007-08-01 00:00:00

abstract：:With an improved N-banding technique, the location of nucleolar organizing region was determined in 27 kinds of material including mammals, a marsupial, birds, amphibians, fishes, an insect and plants. In most cases the N-bands were clearly located on certain specific regions of chromosomes, such as the secondary cons...

journal_title：Chromosoma

authors： Funaki K,Matsui S,Sasaki M

更新日期：1975-01-01 00:00:00

abstract：:The ribosomal DNA(rDNA)-containing chromatin in eukaryotes forms a unique architecture called the "secondary constriction" or "nucleolus organiser region (NOR)" on mitotic chromosomes. To gain more insight into non-histone chromosomal proteins (NHCP), termed "N-band proteins", that are specifically associated with the...

journal_title：Chromosoma

authors： Matsui S,Fuke M,Chai L,Sandberg AA,Elassouli S

更新日期：1986-01-01 00:00:00

abstract：:Mutation of the telomeric repeat sequence has severe cellular consequences in a variety of systems. A Tetrahymena thermophila telomerase template mutant, ter1-43AA, displays an acute mitotic chromosome segregation defect. In the study described here we investigated the molecular basis for this lethality. Although clon...

journal_title：Chromosoma

authors： Petcherskaia M,McGuire JM,Pherson JM,Kirk KE

更新日期：2003-04-01 00:00:00

abstract：:We determined the structure of the Schizosaccharomyces pombe centromere cen3 using direct genomic mapping and cosmid walking. The repetitive region of cen3 is approximately 110 kb, much longer than that of the previously determined cen1 and cen2 regions. The approximately 30 kb long left and approximately 60 kb right ...

journal_title：Chromosoma

authors： Murakami S,Matsumoto T,Niwa O,Yanagida M

更新日期：1991-12-01 00:00:00